• Journal of Environmental Health Sciences
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• v.26 no.4
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• pp.88-96
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• 2000

The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors ere used to test used to test which potential transport system might be involoved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4',4-diisothiocyano-2',2-stilbenedisulfonic acid(DIDS) the Cl- ion transport system and verapamil for the $Ca^{2+}$ transprot system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in ENT-6 cells. In the condition of ouabain containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but ather inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of MeHg deoended on the concentration of added ouabain to the culture medium for MET-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.x.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.435-441
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• 1998

Optimal conditions for the production of natural color, betacyanin were investigated by varying light intensity, C/N ratio, concentrations of phosphate and kinds of elicitors. Batch cultivation was employed to characterize cell growth and betacyanin production of 32 days. The maximum specific growth rate, ${\mu}$$\sub$max/, was 0.3 (1/day) for batch cultivation. The maximum specific production rate, q$\^$max/$\sub$p/, was enhanced 0.11 (mg/g-cell/day) at 3 klux. A light intensity of 3 klux was shown to the best for both cell growth and betacyanin production. The maximum specific production rate was 0.125 (mg/g-cell/day) at 0.242 (1/day), the maximum specific growth rate. The dependence of specific growth rate on the light lintensity is fit to the photoinhibition model. The correlation between ${\mu}$ and q$\sub$p/ showed that the product formation parameters, ${\alpha}$ and ${\beta}$$\sub$p/ were 0.3756 (mg/cell) and 0.001 (mg/g-cell/day), respectively. The betacyanin production was partially cell growth related process, which is different from the production of a typical product in plant cell cultures. In C/N ratio experiment, high carbon concentration, 42.1 (w/w) improved cell growth rate while lower concentration, 31.6 (w/w) increased the betacyanin production rate. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.26 (1/day) and 0.075 (mg/g-cell/day), respectively. Beta vulgaris L. cells under 1.25 mM phosphate concentration produced 10.15 mg/L betacyanin with 13.46 (g-dry wt./L) of maximum cell density. The production of betacyanin was elongated by adding 0.1 ${\mu}$M of kinetin. This also increased the cell growth. Optimum culture conditions of light intensity, C/N, phosphate concentration were obtained as 5.5 klux, 27 (w/w), 1.25 mM, respectively by the response surface methodology. The maximum cell density, X$\sub$max/, and maximum production, P$\sub$max/, in optimized conditions were 16 (g-dry wt./L), 12.5 (mg/L) which were higher than 8 (g-dry wt./L), 4.48 (mg/L) in normal conditions. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.376 (1/day) and 0.134 (mg/g-cell/day) at the optimal condition. The overall results may be useful in scaling up hairy root cell culture system for commercial production of betacyanin.

• Korean Journal of Heritage: History & Science
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• v.46 no.3
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• pp.172-193
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• 2013

This study examines the production techniques and raw materials shown in the Korean dried-lacquer statues of Buddha through a careful observation of the Seated Dried-lacquer Bodhisattva Statue from the late Goryeo Dynasty which is currently possessed by Okura Museum of Art in Tokyo. As a method of study, the X-ray data and the results from a field survey were combined to analyze the production techniques and the characteristics of raw materials. Based on this analysis, a hypothesis was established on the production process and verified through a reenactment of the actual production process. Then, the characteristics of the techniques applied to each process and the raw materials were recorded in detail. Specifically, the dried lacquer techniques and the raw materials were estimated based on the results of naked-eye observation in comparison with the literature, especially the records of "Xiu Shi Lu" written by Huang Cheng of the Ming Dynasty which is considered as 'the textbook of lacquer techniques.' The raw materials used in the production of the traditional Korean lacquerware inlaid with mother-of-pearl were also referenced. As a result, it was found that the features of production techniques and the raw materials found in the Statue at Okura Museum of Art have many similarities with those of the Seated Dried-lacquer Statue of Lohan (Arhat) from Yuanfu 2 Nian Ming (1098) of the Song Dynasty which is currently at the Honolulu Museum of Art. In particular, the similarities include that the interior of the statue being vacant because the clay and the wood core were not replaced after being removed from the prototype, that the complete form was made in the clay forming stage to apply the lacquer with baste fiber fabric, that the clay and the wood core were removed through the bottom of the statue, and that the modeling stage was omitted and the final coat over the statue is very thin. Additionally, decorating with ornaments like Bobal and Youngrak made of plastic material was a technique widely popular in the Song Dynasty, suggesting that the Seated Dried-lacquer Bodhisattva Statue in Okura Museum of Art was greatly affected by the production techniques of the Dried-lacquer Buddha Statue from the Song Dynasty. There is no precise record on the origin and history of the Korean Dried-lacquer Buddha Statues and the number of existing works is also very limited. Even the records in "Xuanhe Fengshi Gaoli Tujing" that tells us about the origin of the Dried-lacquer Buddha Statue from the Yuan Feng Period (1078~1085) do not indicate the time of transmission. It is also difficult to trace the clear route of transmission of production techniques through existing Dried-lacquer Buddha Statues. Fortunately, this study could at least reveal that the existing Dried-lacquer Buddha Statues of Korea, including the one at Okura Museum of Art, have applied the production techniques rather differently from those used in the production of Japanese Datsukatsu Dried-lacquer Buddha Statues that have been known as the standard rule in making dried-lacquer statues of Buddha for a long time.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.1048-1053
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• 2009

The present study was conducted to determine the effects of chitosan on nitric oxide (NO) content and inducible nitric oxide synthase (iNOS) activity in serum, and relative expression of iNOS mRNA in the duodenum, jejunum, and ileum of broiler chickens. A total of 240 one-day-old Arbor Acre mixed-sex broiler chickens were randomly allotted to six dietary treatments with five replicates in each treatment and eight chickens in each replicate. The broiler chickens in the six treatments were fed the basal diet supplemented with 0 (control), 0.05, 0.2, 0.5, 1.0 or 2.0 g/kg chitosan. The trial lasted for 42 days. The results showed that dietary chitosan enhanced NO content and iNOS activity in serum as well as iNOS mRNA expression in the duodenum and ileum of broiler chickens in a quadratic dose-dependent manner (p<0.05), and improved jejunum iNOS mRNA expression in a quadratic dose-dependent manner (p<0.10) with increasing addition of chitosan. Chicks fed a diet containing 0.5-1.0 g/kg chitosan had higher NO content and iNOS activity in serum as well as small-intestinal iNOS mRNA expression compared with birds given the control diet, but positive effects of chitosan tended to be suppressed when addition of chitosan in the diet was increased to 2.0 g/kg. These results implied that there was a threshold level of chitosan inclusion beyond which progressive reductions in serum NO content and small intestinal iNOS expression occured, and the regulation of chitosan on immune functions in chickens is probably associated with activated expression of iNOS and NO secretion.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.1
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• pp.46-52
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• 2000

Two experiments were conducted to determine ileal digestibilities for the amino acids contained in rapeseed meal using the regression technique and then applying the values obtained, in a growth trial, using growing-finishing pigs. For the digestibility trial, four 20 kg crossbred $(Yorkshire{\times}Landrace{\times}Beijing\;Black)$ barrows were fitted with simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75% rapeseed meal. For the growth trial, 80 crossbred $(Yorkshire{\times}Landrace{\times}Beijing\;Black)$ growing pigs $(20{\pm}2.4kg)$ were fed corn-soybean meal diets supplemented with 0, 3, 6, 9 or 12% rapeseed meal. Four pens (2 gilts and 2 castrates) were assigned to each treatment. With the exception of isoleucine and methionine, the digestibility coefficients for the indispensible amino acids declined as the level of rapeseed meal in the diet increased. There was little agreement between the amino acid digestibilities determined with the regression technique and values previously published for rapeseed meal. During the growing (22-42 kg) period, the addition of rapeseed meal had no significant effects on gain, feed intake or feed conversion. During the finishing period (58-91 kg), daily gain was not affected by rapeseed meal inclusion but feed conversion declined (p<0.04) as the level of rapeseed meal in the diet increased.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.635-643
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• 2016

Wine consumers desire to drink a high quality wine. For producing high quality wine, high quality soil is required. Conventionally, soil quality is assessed qualitatively. Using traditional laboratory methods, quantitative data can be obtained for management purpose, but it is time consuming and expensive. Therefore, new technology aims to address these limitations, namely portable X-Ray fluorescence spectrometers (pXRF). This instrument can be used directly in the field, requires no soil sample preparations, and can simultaneously measure a wide range of elements qualitatively that are useful for pedological studies. The chemical composition (Ca, Fe, Ti and Zr) of soils at Tallavera Grove vineyard in New South Wales, Australia, was studied using a pXRF. The analysis of the soil's elemental concentration (i.e. Ca and Fe) using pXRF supports management decisions. Measuring the soil's Ca concentration can be used to identify Ca-rich parent materials (limestone). The limestone indicates good soil conditions for vine production. Fe content was used to identify areas of texture-contrast soils or soil with accumulation of clays in the B horizon. In addition, a soil weathering index was calculated using elemental concentrations (i.e. Ti and Zr) to explore the history of soil formation for making decision of management. This index showed that the soil in the vineyard was affected by two processes: the deposition of materials from elsewhere (Aeolian transport or soil erosion) and mixing of materials from upslope.

• Korean Journal of Digital Imaging in Medicine
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• v.9 no.2
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• pp.5-9
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• 2007

As developing the medical treatment image portion with the change of these times, PACS, which is able to digitalize image portion data, has a lot of data-based image data. Applying this PACS, we would like to settle down RNSXI(real-name shooting X -ray of inspector) system. We interviewed with P ACS's operators of university hospitals which is using PACS in Seoul about the present conditions whether using of RNSXI or not. And we inquired the RNSXI equipments, applying PACS database, and Interface conditions undertook to do in our hospital. All university hospitals in Seoul are set up the P ACS system. But no hospital use the RNSXI. In our hospital, we can check inspector' name or initials who exposure x-ray with the PACS Viewer by looking over equipments(CR, DR, US, MG, MR, CT) and Interface of the DICOM Header data. However, some equipments like RF and Angio can not check inspector' name or initials. Under the Film/System environment, RNSXI system has been used frequently like that inspector's signature or initial added to a patient data. Though the digital medical treatment was developed, RNSXI system was declined. It is necessary to using RNSXI system in order to improving radiologists' rights, even if it is not under the application of the medical treatment image laws. If RNSXI system use, radiologists should specialize in their major and the Repeat rate should be reduced. In environment of PACS, RNSXI system can be used by linking both the equipments and the Interface with a production enterprise of P ACS. Therefore RNSXI system applying the P ACS datebase should settle down in our medical system for being provided lots of data.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.12
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• pp.1791-1797
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• 2007

A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.171-180
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• 2001

Compared to other gene transfer system, the advantages of retrovirus-mediated gene transfer are technical ease, efficient expression and genetic stability. Despite the high potency of the retrovirus vector system in gene transfer, one of the drawbacks is a difficulty in concentration of virus stock. To overcome this problem, we tested a new retrovirus vector system producing the progeny retrovirus particles encapsidated with VSV-G (vesicular stomatitis virus G glycoprotein). The infectivity of this virus was not sacrificed by ultracentrifugal concentration and the host cell range extended from all mammalian to fish embryos. Virus titer after 1,000 x concentration was more than 10$^{8}$ CFU/ $m\ell$ on most of the target cell lines. We applied this pantropic viruses in transgenic chicken production by injecting the concentrated (100$\times$) stock into subgerminal cavity of stage X chicken embryos. The survival rate of chicken embryos after injection was about 20% and gene integration rate in surviving embryos was scored almost 100%. Analyses of RT-PCR and fluorescence microscopy, however, showed no evidence of the transgene expression.

• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.497-506
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• 2019

The aim of this study was to estimate the factors that can be used as a predictive indicator of antler productivity in elk. Body weight and antler data from 115 stags raised at the Animal Genetic Resources Research Center in Korea were used in this study. The function for the average antler weight was derived by age, which was $y=-3.4693+5.3747x-0.8x^2+0.0424x^3$, and $R^2$ was 0.991. The analysis of the relationship between body weight at birth and the 1st and 2nd year and the antler weight after the 2nd year showed a correlation between them. There was a correlation between the antler weight of 1 and 2 year old stags and the weight of 2 year old stags as well; especially, both antler weights for the 1st and 2nd year were highly correlated with the antler weight of the 4th year. However, there was no relationship between the antler main beam length for the 1st and 2nd year and the antler weight after the 3rd year. Therefore, it is expected that a large amount of antler will be obtained in the future if proper management is carried out for young deer from birth to 2 years old. Additionally, the results of this study are expected to be useful for the development of a deer breeding program and the deer industry.