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Inhibitory Effect of High Temperature on the Multiplication of Flacherie Virus in the Silkworm, Bombyx mori L. (바이러스성 건화병의 고온에 의한 발병역제 효과)

  • 김권영;강석권
    • Journal of Sericultural and Entomological Science
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    • v.29 no.2
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    • pp.44-50
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    • 1987
  • The silkworms were inoculated with flacherie virus and reserved at high temperature (37$^{\circ}C$) to find out the effect of high temperature on the multiplication of the virus in silkworms. The obtained results are summarized as follows. 1. It was shown that the inhibitory effect of high temperature treatment on the virus multiplication was achieved at high level at the newly molted stages in 5th instar larvae and both in 4th and in 5th larvae. 2. Twelve hours treatment and 24 hours treatment showed high inhibitory effect on the multiplication of flacherie virus. 3. The silkworms were inoculated with flacherie virus at the newly molted stage in 2nd instar larvae and followed by have temperature (37$^{\circ}C$) treatment at the newly molted stages in 5th instar and both in 4th and in 5th instar for 12 and 24 hours respectively. The increase in the survival rate with high temperature treatment ranged from 33.8 to 39.5 percent in index number and cocoon productive was 45.8 to 54.2 percent compared to non-treatment. 5. In the inoculation of the virus to the 4th instar larvae, the high temperature treatment at the newly molted stage in 5th instar brought increases in the survial rate from 49.7 to 68.9 percent in index number and in cocoon production from 44.3 to 69.0 percent.

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A Study on the Determination of Exterior Orientation of SPOT Imagery (SPOT 위성영상(衛星映像)의 외부표정요소(外部標定要素) 결정(決定)에 관한 연구(硏究))

  • Yeu, Bock Mo;Cho, Gi Sung;Kwon, Hyon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.10 no.4
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    • pp.77-85
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    • 1990
  • The application of remote sensing in small scale mapping has recently been widened to various fields such as information analysis of landuse, environmental conservation and natural resources. SPOT imagery, in particular, offers data which can be processed for 3-dimensional point determination. This is made possible by its high resolution, appropriate swatch width/altitude ratio and stereo imaging capabilities. This study aims to develop a suitable polymonial and an algorithm in the determination of exterior orientation which is essential in the 3-dimensional point determination of SPOT imgery. An algorithm is presented in this study to determine the exterior orientation of a preprocessed level lB film of the satellite image. It was found that a polynominal of 15 parameters is the best fit polynominal for exterior orientation determination, where 1st order line function is used for positon ($X_o$, $Y_o$, $Z_o$) and 2nd order line function is used for orientation (${\kappa}_o$, ${\phi}_o$, ${\omega}_o$).

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Clonal Selection in Early Potato Breeding Program and Determination of Plant Maturity by Using a Plug Culture System (감자(Solanum tuberosum L.)의 육종 초기세대에서 플러그 육묘법을 이용한 계통선발 및 숙기 구분)

  • Kim, Kwan-Soo;Cho, Hyun-Mook;Park, Young-Eun;Lim, Hak-Tae;Song, Yoong-Narm
    • Horticultural Science & Technology
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    • v.19 no.3
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    • pp.329-333
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    • 2001
  • The study was performed to investigate the effect of a plug culture method in the early stage of a potato breeding program. The 105, 200 and 288-cell plug trays were used for raising potato seedlings. The % germination in plug trays ranged between 94 and 96%. Percent tuberization measured at 50 and 60, and 70 days after sowing was the greatest in 105-cell and 200-cell trays, respectively. However, tuberization was most delayed in 288-cell trays. Thus, it is considered that 105-cell tray is the most effective for the selection of early maturing plants. Plant maturity was determined by the morphological characteristics at the seedling stage of stolons cultured in plug trays. Most of seedlings with shorter stolons at seedling stage (1st generation) were the early-maturing, while most of seedlings with longer stolons were the late-maturing at the clonal stage (2nd generation). These results mean that the plant maturity in the early potato breeding program can be effectively grouped by observing the morphological characteristics of stolons after plug culture.

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Characterization of Membrane Fouling and It's Optimal Chemical Cleaning Method in MF Process using D dam water (D댐수를 이용한 정밀여과 공정에서 막오염 특성 및 최적 화학세정방법 조사)

  • Kim, Chung H.;Lim, Jae L.;Lee, Byung G.;Chae, Seon H.;Park, Min G.;Park, Sang H.
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.5
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    • pp.559-569
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    • 2007
  • The purposes of this study were to find the main foulant of membrane and the optimal chemical cleaning method for MF(microfiltration) drinking water treatment system using D dam water as water source. The MF pilot plant which can treat maximum $500m^3/d$ consisted of 3 racks and was operated for 10 months under various operation conditions. After 10 months operation, $1^{st}$ and $2^{nd}$ rack of membrane pilot plant system were cleaned chemically and the degree of the restoration of the fouled membrane in terms of the pure water flux was detemnined. Inorganic compounds which contained in chemical cleaning waste was analyzed by Inductively Coupled Plasma (ICP). One membrane module for 3rd rack was disjointed and membrane fouling materials, especially inorganic compounds were investigated by Electron Probe Microanlysis (EPMA) to elucidate the reason of TMP increase. And also, the various chemical reagents (1N HCl or $H_2SO_4$, oxalic acid as acid and 0.3% NaOCl as alkali) were tested by combination of acid and alkali to determine the optimal chemical cleaning method for the MF system using micro-modules manufactured using the disjointed module. It was verified that the inside and outside of membrane module was colorized with black. As a result of the quantitative and semi-qualitative analysis of membrane foulant by ICP, most of inorganic foulant was manganese which is hard to remove by inorganic acid such as HCI. Especially, it was observed by EPMA that Mn was attached more seriously in inside surface of membrane than in outside surface of that. It was supposed that Mn fouling in inside surface of membrane might be caused by the oxidation of soluble manganese (Mn(II)) to insoluble manganese ($MnO_2$) by chlorine containing in backwashing water. The optimal cleaning method for the removal of manganese fouling was consecutive cleaning with the mixture of 1N HCl and 1% of oxalic acid, 0.3% NaOCl, and 1N HCl showing 91% of the restoration of the fouled membrane.

A study on the effects of the storage molds on the biology of the rice weevil, Sitophilus oryzae L. (쌀바구미의 생육에 미치는 Aspergillus spp.의 영향)

  • Hyun Jai Sun;Yu Moon Il
    • Korean journal of applied entomology
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    • v.13 no.2 s.19
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    • pp.71-75
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    • 1974
  • A study was conducted to investigate the relationships between the rice weevil and associated storage molds. The results are as follows; 1. All of the developmental stages of the rice weevils are carrying some storage molds in their bodies, and the order of magnitute in the number was the adult, larva and pupa. 2. The molds persist in the body of the rice weevil for 10 days who.1 they were fed on the mold free wheat, and the most persistant mold species was A. candidus. 3. When the mold free weevils were reared on the pure culture of the molds on the wheat, the number of eggs laid by the Lveevil were the greatest for A. candidus. following A. ruber, and the . least number Lvere obtained with A. niger 4. The rice weevil could complete in the pure mold culture on the wheat except for A. niger where the larvae had developed by 2nd or 3rd instars. 3. The shortest developmental periods was obtained with A. candidus and the first adult emerged in 4th week. 6. The unfavorable effects of A. niger on the development of the rice weevil might be associated with the fast growth of the mold together with some unknown effects. 7. There seems to be a protocooperative interaction between these two oraganisms having been developed through the long evolutionary course in common habitat.

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Injury Aspects of the Stone Leek Leafminer, Liriomyza chinensis Kato (Diptera: Agromyzidae) on Welsh Onion (파를 가해하는 파굴파리의 충태별 피해 양상 및 행동)

  • 최인후;김정화;김길하;김철우
    • Korean journal of applied entomology
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    • v.42 no.4
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    • pp.335-343
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    • 2003
  • An experiment was conducted to investigate types of injury inflicted by the stone leek leafminer, Liriomyza chinensis Kato (Diptera: Agromyzidae) on welsh onion. A feeding scar made by an adult female was a hole round in shape, with diameter of 0.08 mm and 0.48 mm in lesion, resulting in a white spot, many of which often form vertical dotted lines on a leaf. Egg spots were oval with 0.1 ${\times}$0.14 mm in size, one or several of which often form a V-shape in group. Feeding by adults began immediately after emergence and was very active from 4th to 5th day. Oviposition was done from 2nd to 6th day after emergence. In both feeding and oviposition, they were more active in the day time. Larvae after emergence crawled up the leaf at first, and then moved up and down to feed on mesophyll. When in high density, they feed on leaf from leaf tip to bottom, and let the leaf die. Area of damage per one larva was calculated as 72.1 $\textrm{mm}^2$. The aged larvae escaped from the leaf in early morning, usually between 5 and 7 am. Most pupation sites were distributed near plants,5cm in soil depth and within 10 cm away from the plant. Pupae of L. chinensis overwintered 10cm below soil surface and emerged from early May to late June the next year Adults then moved to welsh onions near over wintering sites, nursery, transplanted, and levee.

Guided Tissue Regeneration Using Barrier Membrane and Osseous Grafts in Surgically Created Furcation Defects in Dogs (성견의 외과적 치근이개부 골결손에 차폐막과 골이식재를 이용한 조직유도재생술시 치유양상)

  • Chung, Eun-Hee;Chung, Hyun-Ju
    • Journal of Periodontal and Implant Science
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    • v.26 no.4
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    • pp.967-987
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    • 1996
  • The present study was to evaluate the healing patterns of guided tissue regeneration( GTR) using resorbable $Vicryl^{(R)}$(polyglactin 910) mesh and nonresorbable expanded polytetrafluoroethylene(ePTFE) membrane with or without bone grafting using autogeneous bone and demineralized freeze-dried bone allograft(DFDBA) in the grade II furcation defects. Mucoperiosteal flaps were reflected buccally in the mandibular 2nd, 3rd and 4th premolar areas and furcation defects were created surgically by removing $5{\times}6mm$ alveolar bone in 4 dogs. Root surfaces were thoroughly debrided of periodontal ligament and cementum, and notches were placed on root surface at the most apical bone level. In the right and left mandibular quadrant, each tooth was received $Vicryl^{(R)}$ mesh(ACE Surgical Supply Co., USA) only, $Vicryl^{(R)}$ mesh with DFDBA, $Vicryl^{(R)}$ mesh with autogeneous bone grafts, ePTFE membrane($Core-tex^{(R)}$ membrane, W.L. Gore & Associates Inc., USA) only, ePTFE membrane with DFDBA or ePTFE membrane with autogeneous bone grafts. For the fluorescent microscopic examination, fluorescent agents were injected at 2, 4 and 8 weeks after surgery. Four weeks after surgery, 2 dogs were sacrificed and ePTFE membranes were removed from remaining 2 dogs, which were sacrificed at 12 weeks after surgery. Undecalcified tissues were embedded in methylmethacrylate and $10{\mu}m$ thick sections were cut in a buccolingual direction. These sections were stained with hematoxylin-eosin stain and Masson's trichrome stain, and evaluated by descriptive histology and linear measurements. The results were as follows : 1) $Vicryl^{(R)}$ mesh group showed less connective tissue attachment than ePTFE membrane group. 2) The combination of GTR using $Vicryl^{(R)}$ mesh and osseous grafts resulted in new attachment and new bone formation more than GTR using $Vicryl^{(R)}$ mesh only. 3) GTR using ePTFE membrane, with or without osseous grafts, enhanced periodontal regeneration. 4) Root resorption and dentoalveolar ankylosis were observed in the areas treated with the combination of GTR and DFDBA. It was suggested that the effect of adjunctive bone grafting in GTR procedure depends on the materials and the physical properties of barrier membranes. $Vicryl^{(R)}$ mesh performed a barrier function and the use of adjunctive bone grafting may enhance the periodontal regeneration.

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Effect of Supplemental Lighting of Sub-Compensation Intensities on Growth of Rosa hybrida L. 'Vital' (광보상점 이하의 보광이 절화장미 'Vital'의 생육에 미치는 영향)

  • Choi, Young-Hah;Kwon, Joon-Kook;Choi, Gyeong-Lee;Kang, Nam-Jun;Chun, Hee;Cho, Myeong-Whan;Seo, Tae-Cheol;Roh, Mi-Young;Lee, Seong-Chun;Lee, Jae-Han
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.132-137
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    • 2008
  • This experiment was conducted to investigate the effect of supplemental lighting of low light intensities on growth and yield of rose 'Vital' in a forcing culture. Metal halide lamp (MH), High pressure sodium lamp (HSP), and MH+HSP were used as the light sources, and they were set up at a $310\;cm{\times}450\;cm$ interval and at 120cm above the culture beds. Light intensity at 1m point distance from supplemental lighting sources was $32{\sim}34\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Days to the 1st and 2nd harvests decreased by $5{\sim}8$ and $3{\sim}5$days, respectively in supplemental lighting treatment as compared to the control. Days to harvesting was the shortest in MH+HPS treatment, followed by BPS and MH, although there was no significant difference between HPS and MH treatments. The growth was better and incidence of blind shooting decreased by $5{\sim}7%$ in supplemental lighting treatments than the control, increasing marketable cut flowers. The incidence of blind shoot was the lowest in MH+BPS treatment, and there were no significant difference between MH and BPS treatments. In conclusion, supplemental lighting of low light intensities was effective in reducing days to flowering and reduced occurrence of blind shoots.

The Clinical Study on 77 Traffic Accident Patients (교통사고 환자 77례에 대한 임상적 고찰)

  • Hwang, Hee-Sang;Park, Jae-Yeon;Kim, Min-Seok;Jeon, Jae-Cheon;Jung, Ki-Hoon;Lee, Tae-Ho;Roh, Jeong-Du;Lee, Eun-Yong
    • The Journal of Churna Manual Medicine for Spine and Nerves
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    • v.4 no.2
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    • pp.85-98
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    • 2009
  • Objectives : The purpose of this study was to find out tendency of traffic accident patients treated in korean medicine hospital. Methods : We investigated 77 patients who were treated in Semyung University Korean Medicine Hospital from 1 st, January, 2007 to the 30st September, 2009. Results and Conclusions : 1. In distribution of gender and age, the rate of male was 36.4%, that of female was 63.6% and majority of patients were twenties, thirties and forties. 2. In the traffic accident patterns, the most was car crash from behind(49.4%) and after traffic accident, 42 patients(54.6%) visited our hospital in less than 5 days. 3. 22 patients(28.6%) choose the oriental medical treatment for primary care. And 35 patients(45.4%) visited our hospital due to preference of oriental medicine. 4. In partial pain, most was the neck(76.6%), followed by low back(61.0%). 5. In radiation test(excluded sprain and straightening), the most was HIVD of L-SPINE(14.0%). 6. In the periods of admission, less than 7 days was the 1st(36.4%), 14 days was the 2nd(27.3%). 7. The treatment was effective to 67 patients out of 77 patients who had been hospitalized.

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Evaluation of the degree of maturity of Chinese cabbage blades and midribs pretreated with dilute acetic acid solutions during Kimchi fermentation (초산 전처리 배추김치의 발효중 엽신 및 중륵별 김치숙성도 평가)

  • 정대림;이혜준;우순자
    • Korean journal of food and cookery science
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    • v.11 no.1
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    • pp.37-43
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    • 1995
  • Effect of the pretreatment with acetic acid(0.01%, 0.05%, 0.1%) on the fermentation of Chinese cabbage Kimchi, and differences in the degree of maturity between Chinese cabbage blades and midribs were observed. To estimate the degree of maturity, correlation analysis was used with several variables such as pH, titratable acidity, volatile acidity, ascorbic acid content, resazurin test value and pH/acidity ratio. Based on the pH and titratable acidity, the degree of maturity of Chinese cabbage midribs fermented at 10$^{\circ}C$ until the optimum stage of fermentation increased more rapidly than those of the blades. The stage of maturity of Kimchi pretreated with 0.01% acetic acid were slightly prolonged, compared to those pretreated with acetic acid at the higher levels. The pH of Chinese cabbage midribs and blades pretreated with acetic acid was lower than those of control between 2nd and l0th day of fermentation. The volatile acidity of Chinese cabbage midribs and blades increased proportionally with the concentration of acetic acid. The ascorbic acid contents of Chinese cabbage midribs showed higher than those of Chinese cabbage blades. During the fermentation, the higher concentration of acetic acid was used, the less ascorbic acid content was remained. The pH/acidity ratio, volatile acidity, ascorbic acid content and resazurin test value of Chinese cabbage midribs and blades during Kimchi fermentation at 10$^{\circ}C$ showed good correlations with the pH and titratable acidity.

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