• Title/Summary/Keyword: $L_1-B_4$

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Plant Regeneration from Leaf and Root Cultures of Lycoris chejuensis via Bulblet Formation (제주상사화 (Lycoris chejuensis K. Tae et S. Ko) 잎 및 뿌리 절편으로부터 소자구 형성을 통한 식물체 재생안)

  • Oh, Myung-Jin;Park, Jong-Mi;Tae, Kyoung-Hwan;Liu, Jang-Ryol;Kim, Suk-Weon
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.223-227
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    • 2007
  • Plant regeneration system from leaf and root segments of Lycoris chejuensis via bulblet formation was established. Surface-sterilized leaf and root segments were cultured on the B5 medium containing 2,4-D. After 12 weeks of culture onto B5 medium containing 2,4-D, white globular structures and white calluses were formed on the cut surface of the explants. The highest frequency of globular structures and calluses formation from leaf explants was 32.1% when leaf explants were cultured onto B5 medium supplemented with 1 mg/L of 2,4-D. However, the higher concentration of 2,4-D (over than 3 mg/L) resulted in decrease of the frequency. In comparison to leaf explants, root segments showed the highest frequency at a rate of 36.1% when root explants were cultured onto B5 medium supplemented with 3 mg/L of 2,4-D. These structures and calluses were sub-cultured and proliferated onto the same culture medium. Upon transfer to B5 basal medium, white globular structures were developed into bulblets and normal plantlets. After 4 weeks of incubation in the light, plantlets were successfully rooted over the frequency of approximately 90%. Rooted plantlets were successfully transferred to potting soil and acclimatized in the growth chamber. The plant regeneration system of Lycoris chejuensis established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.

Development of Vaccinium uliginosum L. extracts for whitening & anti-wrinkle functional food

  • Choung Se-Young
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2005.04a
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    • pp.131-148
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    • 2005
  • This study is peformed to investigate the effect of water extract from Vaccinium uliginosum L., on melanin production in B 16 melanoma cells, procollagen production and matrix metalloproteinase-1(MMP-1) inhibition in human fibroblast cells. One hundred grams of the Vaccinium uliginosum L. was extracted with 2000 mL of water($90^{\circ}C$, 16h, 2times). The water extracts were lyophilized and stored at $4^{\circ}C$ until used. Dry weight yields of extracts of Vaccinium uliginosum L. were $3\%$(w/w). Extracts from Vaccinium uliginosum L. showed scavenger activities on DPPH radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide and singlet oxygen radical. And these substances inhibited release of cyiokines from human keratinocyte after UV B exposure. Therefore we confirmed that extracts from Vaccinium uliginosum L. had antioxidative effect. These substances inhibited purified tyrosinase activity and melanogenesis in B 16 melanoma cells treated/untreated IL-$1{\alpha}$. Moreover this extract stimulated procollagen production and inhibited MMP-1 production in human fibroblast cells treated/untreated IL-$1{\beta}$. Therefore we confirmed that extracts from Vaccinium uliginosum L. had whitening effect. And these substances decreased degree of wrinkle in hairless mouse skin that induced by UV B irradiation. Therefore we confirmed that extracts from Vaccinium uliginosum L. had anti-wrinkle effect. From the above results, it is possible that Vaccinium uliginosum L. may be developed to be an anti-melanogenesis agent and anti-wrinkle agent.

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The Effectiveness of Overflow Improvement of Broad-Crested Side Weirs according to Installing a Hydraulic Structure (보 설치에 따른 광정횡월류위어의 월류량 개선 효과)

  • Kang, Ho Seon;Cho, Hong Je
    • Journal of Korea Water Resources Association
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    • v.48 no.7
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    • pp.523-533
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    • 2015
  • In this study, the effectiveness of overflow improvement of weir was tested by conducting hydraulic experiments at the designated spot for installment of side weir under the condition of installment of hydraulic structures such as small reservoir in mainstream. The height of the reservoir was set up as a third of that of the weir, accordingly the rate of the height of the weir and the distance of the reservoir from the weir($B_h/L_b$) were 0.05, 0.025, 0.0167 each. As a result, overflows per unit width increased by 8.1%, 5.4% and 3.9% perspectively. A new discharge coefficient that adds $B_h/L_b$ as parameter to the existing discharge coefficient of trapezoidal broad crested side weir was suggested and the application of the new formula of discharge coefficient by comparing measured overflow with calculated overflow was identified.

The Relationship between the Progression of Chronic Kidney Disease and Beta Cell Function in Non-Diabetic Korean Adults (대한민국 비당뇨 성인에서 만성신장질환과 인슐린저항성 및 베타세포기능의 관련성)

  • Kim, Hyung Rag
    • Korean Journal of Clinical Laboratory Science
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    • v.52 no.3
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    • pp.165-171
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    • 2020
  • This study examined the relationship between chronic kidney disease (CKD) and the homeostasis model assessment of insulin resistance (HOMA-IR) and beta-cell function (HOMA-B) in non-diabetic Korean adults. This study included 4,380 adults aged 20 or older (50.32±16.14) using the 2015 Korea National Health and Nutrition Examination Survey (KNHANES) data, which represents the national data in Korea. The present study had several key findings. First, in terms of HOMA-IR, after adjusting for the related variables (Model 4), the HOMA-IR (M±SE, 95% confidence interval [CI]) in group 1 (G1; estimated glomerular filtration rate [eGFR], ≥90 mL/min/1.73 ㎡), group 2 (G2; eGFR, 60~89 mL/min/1.73 ㎡), group 3a (G3a; eGFR, 30~59 mL/min/1.73 ㎡), and ≥group 3b (≥G3b; eGFR, <30 mL/min/1.73 ㎡) were 1.78±0.03 (1.73~1.83), 1.87±0.03 (1.81~1.93), 2.16±0.13 (1.91~2.42), and 2.59±0.24 (2.12~3.06), respectively. The HOMA-IR was positively associated with the progression of CKD (P<0.001). Second, in terms of the HOMA-B, after adjusting for the related variables (Model 4), the HOMA-B (M±SE, 95% CI) in G1, G2, G3a, and ≥G3b were 87.46±1.21 (85.08~89.84), 89.11±1.38 (86.40~91.81), 104.82±5.91 (93.23~116.42), and 123.97±10.87 (102.66~145.29), respectively. HOMA-B was positively associated with the progression of CKD (P<0.001). Both insulin resistance and the beta-cell function were positively associated with CKD in non-diabetic Korean adults.

Design and Fabrication of Quadruple Band Antenna with DGS (DGS를 적용한 4중대역 안테나의 설계 및 제작)

  • Kim, Min-Jae;Choi, Tea-Il;Choi, Young-Kyu;Yoon, Joong-Han
    • The Journal of the Korea institute of electronic communication sciences
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    • v.15 no.1
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    • pp.31-38
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    • 2020
  • In this paper, we propose a quadruple band antenna for GPS/WLAN/WiMAX application. The proposed antenna has quadruple band characteristics by considering the interconnection of four strip lines and DGS on the ground place. The total substrate size is 20.0 mm (W1) ⨯27.0 mm (L1), thickness (h) 1.0 mm, and the dielectric constant is 4.4, which is made of 20.0 mm (W2)⨯ 27.0 mm (L8 + L6+ L10) antenna size on the FR-4 substrate. From the fabrication and measurement results, bandwidths of 60 MHz (1.525 to 1.585 GHz) bandwidth for GPS band, 825 MHz (3.31 to 4.135 GHz) bandwidth for WiMAX band and 480 MHz (2.395 to 2.975 GHz) and 385 MHz (5.10 to 5.485 GHz) bandwidth for WLAN band were obtained on the basis of -10 dB. Also, gain and radiation pattern characteristics are measured and shown in the frequency of triple band as required.

A Study on the Drinking Water Treatment by Precoat Filtration and Activated Carbon Adsorption Process (규조토여과 및 활성탄흡착 공정을 이용한 용수처리에 관한 연구)

  • Shin Dae-Yewn;Kim Ji-Yeoul;Ji Sung-Nam
    • Journal of Environmental Health Sciences
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    • v.30 no.5 s.81
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    • pp.402-409
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    • 2004
  • I performed the research about the drinking water treatment by precoat filtration and activated carbon adsorption process in the D water treatment plant at Gwangju. D water treatment plant inlet water is supplied from Juam lake in Jeollanamdo. The results are as follows; 1. Element disk used in this experiment are R(pore size $10{\mu}m$), B(pore size $20{\mu}m$). And diatomaceous earth are A(cake pore size $3.5{\mu}m$), B(cake pore size $7{\mu}m$) and C(cake pore size $17{\mu}m$) 2. Filtrate of precoat filter during 30 min are B-C 10.2 > BB 5.7 > R-A 5.4 ($m^3/m^2$). 3. The water quality through B-C+AC and R-A+AC are DOC 1.76 mg/1, 1.288 m/l respectively. 4. total THMs produced by chlorination are $84.2{\mu}g/l$(B-C+AC), $66.11{\mu}g/l$ (R-A+AC), $97{\mu}g/l$ (rapid sand filtration water) respectively. 5. The R-A+AC and B-C+AC process can be substitute of CWTS.

Production of a Phospholipase C by Bacillus cereus and Its Characterization (Bacillus cereus에 의한 C형 인지질 분해효소의 생산과 특성 고찰)

  • 서국화;임용식;이종일
    • KSBB Journal
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    • v.19 no.4
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    • pp.250-256
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    • 2004
  • In this work we have cultivated several B. cereus strains in a complex LB medium in order to study the production of phospholipase C (PLC), and among them B. cereus 318 showed the highest productivity of PLC. Some components, i.e., 5 g/L glucose, 5 g/L yeast extract, 5 g/L peptone, 0.5∼1.0 g/L K$_2$HPO$_4$, 0.02∼0.04 g/L ZnSO$_4$$.$7H$_2$O and 3 g/L NaHCO$_3$ were found to be optimal for the high production of PLC by B. cereus 318. Optimal culture temperature and pH were found to be 30$^{\circ}C$ and pH 7.5 for the PLC production, respectively. Optimum reaction temperature and pH of the PLC produced by B. cereus 11 and 318 were 45$^{\circ}C$ and pH 4.0, while they were 50$^{\circ}C$ and pH 7.0 for the PLC by B. cereus 559. The PLC produced by B. cereus was activated by Mn$\^$2+/, Co$\^$2+/ and dimethyl sulfoxide (DMSO), but its activity was inhibited by Cu$\^$2+/ and partially by glycerol, isopropanol and sodium dodecyl sulfate (SDS).

Subcloning and Sequencing of Maize rbcL Promoter Region

  • Woong-Seop Sim
    • Journal of Plant Biology
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    • v.38 no.1
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    • pp.107-113
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    • 1995
  • pRLYS1 containing intact rbcL gene of maize (Zea mays L. cv Golden X Bantam T-51; Zm-A) was digested with several restriction enzymes to construct subcones carrying promoter region of rbcL. The DNA fragments of 0.20, 0.19, 0.92 and 1.55 kb among the EcoRI digests, the EcoRI-DdeI digests, the AvaI digests and the EcoRI-BamHI digests of pRLYS1 were subcloned into pBluscriptSK+and named pRLPS2, pRLPS3, pRLPS14 and pRLPS35, respectively. Four subclones contain the 1.92 kb portion from 136 nucleotide downstream to 1780 nucleotide upstream from the ATG initiation codon of rbcL gene. pRLPS2 (-29 to -229) and pRLPS3 (-239 to -420 from the ATG) were sequenced. When nucleotide sequence of Zm-A was compared with sequence of rbcL promoter region of a different cultivar of maize (Zea mays L. cv WFG TMS X BS7; Zm-B), the difference rate between two cultivars was 4.3%. The mean of sequence divergence between Zm-A and three grass species in the same tribe, Andropogoneae, in the upstream region from 29 to 420 of ATG was 1.8%, whereas between Zm-B and above-mentioned three species was 5.4%. Therefore, Zm-A seems to evolutionarily closer to three other species in Andropogoneae tribe than Zm-B is.

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Evaluation and Prediction of Color Changes of Sugar-Glycine Mixtures by Maillard Reaction (마이야르 반응에 의한 당과 글리신 혼합물의 색변화의 평가와 예측)

  • Kim, Woo-Jung;Chun, Young-Hye;Sung, Hyun-Soon
    • Korean Journal of Food Science and Technology
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    • v.18 no.4
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    • pp.306-312
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    • 1986
  • Color changes of a mixture of 0.8 M glucose, fructose or sucrose and 0.8 M glycine were evaluated by Hunter 'L', 'a', 'b' values during Maillard reaction at the temperature range of $70^{\circ}-100^{\circ}C$. The results suggested the reaction to be devided into three stages. In initial reaction the 'L' and 'a' values decreased slowely, and 'b' rapidly increased to maximum, which were followed by a rapid decrease in 'L' and 'b' and rapid increase in 'a' values at intermediate stage. At final reaction period the both 'L' and 'b' were slowed down in their reduction rate while 'a' decreased rapidly from its maximum point. All of the Hunter values were reached to nea. zero. Linear equations, which can be used for prediction of cole. change in Maillared reaction, of $1/\sqrt{(L_t-L_{\circ})^2\;+\;(a_t-a_{\circ})^2\;+\;(b_t-b_{\circ})^2}\;=\;a(1/t)\;+\;b$ for Hunter values and $1/\sqrt{X\;+\;Y\;+Z}\;=\;a(1/t)\;+\;b$ for CIE tristimulus values were found to have a very high correlation coefficient. Relationships between 'L' and 'a', 'L' and 'b' and 'a' and 'b' were also graphcally compared.

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CHARACTERIZATION OF FUNCTIONS VIA COMMUTATORS OF BILINEAR FRACTIONAL INTEGRALS ON MORREY SPACES

  • Mao, Suzhen;Wu, Huoxiong
    • Bulletin of the Korean Mathematical Society
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    • v.53 no.4
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    • pp.1071-1085
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    • 2016
  • For $b{\in}L^1_{loc}({\mathbb{R}}^n)$, let ${\mathcal{I}}_{\alpha}$ be the bilinear fractional integral operator, and $[b,{\mathcal{I}}_{\alpha}]_i$ be the commutator of ${\mathcal{I}}_{\alpha}$ with pointwise multiplication b (i = 1, 2). This paper shows that if the commutator $[b,{\mathcal{I}}_{\alpha}]_i$ for i = 1 or 2 is bounded from the product Morrey spaces $L^{p_1,{\lambda}_1}({\mathbb{R}}^n){\times}L^{p_2,{\lambda}_2}({\mathbb{R}}^n)$ to the Morrey space $L^{q,{\lambda}}({\mathbb{R}}^n)$ for some suitable indexes ${\lambda}$, ${\lambda}_1$, ${\lambda}_2$ and $p_1$, $p_2$, q, then $b{\in}BMO({\mathbb{R}}^n)$, as well as that the compactness of $[b,{\mathcal{I}}_{\alpha}]_i$ for i = 1 or 2 from $L^{p_1,{\lambda}_1}({\mathbb{R}}^n){\times}L^{p_2,{\lambda}_2}({\mathbb{R}}^n)$ to $L^{q,{\lambda}}({\mathbb{R}}^n)$ implies that $b{\in}CMO({\mathbb{R}}^n)$ (the closure in $BMO({\mathbb{R}}^n)$of the space of $C^{\infty}({\mathbb{R}}^n)$ functions with compact support). These results together with some previous ones give a new characterization of $BMO({\mathbb{R}}^n)$ functions or $CMO({\mathbb{R}}^n)$ functions in essential ways.