• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.125-131
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• 2000

Protoplasts of Arabidopsis thaliana were easily isolated from the shoot-forming (SF) suspension-cultured cell clusters with 4 hours-shaking condition (40 rpm) on CPD enzyme solution containing 1% cellulase R-10, 0.25% pectolyase Y-23 and 0.5% driselase. Protoplasts were cultured on liquid KAO medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L kinetin, 200 mg/L spermidine and 68 g/L glucose. Also, protoplasts were cultured on 0.2 $\mu$M membrane filter placed onto CP solid medium containing the suspension cells as feeder cells in the dark at $25^{\circ}C$ for 4 weeks. Protoplast-derived-SF calli were cultured on MS medium containing 0.05 mg/L IAA, 7 mg/L 2 ip and 30 g/L sucrose under the continuous illumination for four weeks. The frequency of shoot formation was about 60%. The regenerants were transferred into potting soil to grow mature plants. The regenerants formed the silques with seeds after 8 weeks of cultures.

• Korean Journal of Environmental Agriculture
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• v.20 no.2
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• pp.112-115
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• 2001

Salt accumulation and movement by runoff ware studied in runoff resevior lysimeter constructed in a green house located in the area of Cheongju, Chungbuk province. Average runoff ratio of rainfall within period of this experiment was 58%. The average content of cations lost from field soil by runoff was in the order of $Ca^{2+}(27.12\;mg/L\;)$ > $K^+(9.18\;mg/L)$ > $Mg^{2+}(2.53\;mg/L)$ > $Na^+(1.89\;mg/L)$ and in the care anions $SO_4\;^{2-}(63.38\;mg\;/L)$ > $NO_3\;^-(25.40\;mg/L)$ > $Cl^-(4.19\;mg/L)$ > $PO_4\;^{3-}(3.18\;mg/L)$. Amounts of salt movement by runoff $SO_4\;^{2-}(140.2\;kg/ha)$, $Ca^{2+}(59.9\;kg/ha)$, $NO_3\;^-(56.1\;kg/ha)$, $K^+(20.3\;kg/ha)$, $Cl^-(9.3\;kg/ha)$, $PO_4\;^{3-}(7.0\;kg/ha)$, $Mg^{2+}(5.6\;kg/ha)$ and $Na^+(4.2\;kg/ha)$. The loss amount of $PO_4\;^{3-}$ was the lowest among the anions investigated in this experiment. $P_2O_5$ was accumulated on the soil surface due to strong affinity for the sorption site on the soil particle surface.

• Journal of Advanced Marine Engineering and Technology
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• v.21 no.4
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• pp.430-436
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• 1997

Time-dependent Flow characteristics of a two-dimensional lid-driven square cavity flow of six high Reynolds numbers, $10^4 2{\times}lO^4., 3{\times}l0^4, 5{\times}lO^4, 7.5{\times}lO^4$ and $10^5$ were investigated. A convection conservative difference scheme based upon SOLA to maintain the nearly 2nd-order spatial accuracy was adopted on irregular grid formation. Irregular grid number is $80{\times}80$ and its minimum size is about 1/400 of the cavity height(H) and its maximum is about 1/53 H. The result shows that at Re= $10^4$, periodic migration of small eddies appearing in corner separation region and its temporal sinusoidal fluctuation are represented. And another critical Reynolds number which shows the commencement of flow randomness emerging from the periodic fluctuation is assumed to be around Re= $1.5{\times}10$. At five higher Reynolds numbers ($2{\times}lO^4., 3{\times}l0^4, 5{\times}lO^4, 7.5{\times}lO^4$ and $10^5$), an organizing structure of four consecutive vortices similar to a Moffat vortex at two lower corners is revealed from time-mean flow patterns.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.49-55
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• 1997

This study was conducted to establish mass propagation system from the tissue culture using immature embryos in Eleutherococcus senticosus. Immature embryos from seeds were removed under the microscope and placed on MS, $MSB_5\;and\;B_5$ medium containing several plant growth regulators. While the calli were well formed on media containing 2 mg/l of 2, 4-D, 2 mg/l of 2, 4-D and 0.7 mg/l of TDZ, shoot regeneration was better on MS medium with combinations of high concentrations of TDZ and low concentrations of 2, 4-D. Treatment of 2, 4-D alone was better than treatment of TDZ alone in callus induction, but plant regeneration was reversed. The results of callus formation and shoot regeneration on $MSB_5\;and\;B_5$ media were similar to those of MS media. The rate of callus formation was nearly 100% when 2, 4-D was added to $B_5$, medium on concentration of 2 mg/l or 0.7 mg/l. TDZ showed very significant effect on the formation of multiple shoots.

• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.50-55
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• 2011

The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.6 no.2
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• pp.53-60
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• 2011

Objectives : The purpose of this study is to find out the relation between the height of intercrestal line, and HIVD(Herniated Intervertebral Disc)of Lumbar spine. Methods : We investigated 445 cases (208 male, 237 female) of patients who were diagnosed as HIVD of L-spine at either L4/5 or L5/S1 level. We analysed the relation between the height of intercrestal line and the level of HIVD. Results : 1. Among 445 cases, the level of intercrestal line with HIVD of L-spine at the specific location was different between male and female. In male, the intercrestal line is more likely to be located at L4 level, while in female more likely to be located at L5 level. 2. Among 445 cases, L5/S1 HIVD patients(272, 61.1%) were more than L4/5 HIVD patients(173, 38.9%). At L4 body area, L4/5 HIVD patients were more than L5/S1 HIVD patients, however, at L4/5 intervertebral area and L5 body area, L5/S1 HIVD patients were more than L4/5 HIVD patients. (p<0.01) 3. When the line is located at higher level, HIVD of L-spine tends to be occurred at L4/5 level. Contrastly, when the line is located at lower level, HIVD of L-spine tends to be occurred at L5/S1 level. (p<0.01) Conclusions : High intercrestal line leads to L4/5 HIVD, while low intercrestal line leads to L5/S1 HIVD. Possibly, it is caused by different length and thickness of the iliolumbar ligament.

• Horticultural Science & Technology
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• v.16 no.2
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• pp.239-241
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• 1998

The experiments were carried out to improve culture efficiency of rhizome and mericlone propagation through settlement of problems occurring during culture period of temperate Cymbidium species. Shooting efficiency from rhizome of C. forrestii 'Nokwoon' was improved, when cultured in $H_3P_4$ medium (Hyponex 3+peptone 4g/L) supplemented with 170mg/L $NaH_2PO_4{\cdot}H_2O$ and 0.4mg/L Thiamin e HCl, but the other varieties were not influenced to shooting efficiency by additives. Medium in which rhizome of C. nishiuchianum 'Hodukjiwha' was cultured became less browned in $H_3P_4$ medium added with 150mg/L PVP, but the other treatments of antioxidants was failed to prevent the medium browning. Re-formation of rhizome from young shoots of C. forrestii 'Sojub', 5.5cm in length occured in $H_3P_4$ enriched with 2.0 mg/L NAA and 1.0 mg/L BA under darkness, but axillary buds were elongated in the medium with 1.0 mg/L NAA and 3.0 mg/L BA under light condition. On the other hand, rhizomes from young shoot of C. forrestii 'Seosinmae' and 'Songmae', 5.5cm and 2.5cm in length respectively were reformed in 2.0 mg/L NAA and 5.0mg/L kinetin under darkness, but multishoot from young shoot were emerged in 2.0mg/L NAA and 3.0mg/L BA.

• Korean Journal of Poultry Science
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• v.14 no.1
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• pp.55-61
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• 1987

Four treatments were conducted to determine the effects of lighting regimes on the body weight gain and feed conversion of broiler chicks during 7 weeks: 1) 24 hours consistant lighting regime, 2) intermittent lighting regime of 1 hour lighting and 3 hours dark, 3) 20 hours lighting and 4 hours dark regime, 4) natural lighting regime. Each treatment was composed of three replications and 180 broiler male chicks were used in this experiment (45 chicks for each treatment,15 chicks for each replication). The results of this experiment were summarized as follows; 1. The body weight gains of 1 hour lighting＋3 hours dark regime were heavier than those of any other treatments during whole period, but no significant differences were found. In 1-4 weeks, the body weight gains of 1 hour lighting＋3 hours dark regime and 20 hours lighting＋4 hours dark regime were much heavier than those of natural lighting regime and 24 hours consistant lighting regime, but in 5-7 weeks, no differences were found among the 4 treatments. 2. The feed conversions of 1 hour lighting＋3 hours dark regime were improved more than those of any other treatments during whole period, but no significant differences were found. In 1-4 weeks, the feed conversions of 1 hour lighting＋3 hours dark regime and 20 hours lighting＋4 hours dark regime were much more improved than those of natural lighting regime and 24 hours consistant lightine regime, but in 5-7 weeks, no differences were found among the 4 treatments. 3. These results indicated that the intermittent lighting regimes such as 1 hour lighting＋3 hours dark and 20 hours lighting＋4 hours dark are more efficient on the body weight gain and feed conversion of broiler chicks than natural lighting regime and 24 hours consistant lighting regime.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.300-303
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• 2005

대전지역 화강암지대에 존재하는 75개 지하수를 5개 지역구에서 채취하였으며, 상ㅁ하반기 동안 2차례 시료를 채취하여 건기와 우기후의 라돈 및 우라늄 농도를 분석하였다. 5개 지역에 대한 라돈과 우라늄의 평균 농도는 유성구에서 270.9 Bq/L, $43.8{\mu}g/L$ 였으며, 동구의 경우 41.3 Bq/L, $4.9{\mu}g/L$, 대덕구는 131.8 Bq/L, $54.3{\mu}g/L$, 중구의 경우 44.0 Bq/L, $8.1{\mu}g/L$ 그리고 서구는 112.9 Bq/L, $0.4{\mu}g/L$ 이었다. 라돈과 우라늄의 함량은 건기가 우기후에 비해 대체로 높게 나타났으며 건기시의 평균값은 라돈은 $253{\pm}14\;Bq/L$ 우라늄은 $63{\mu}g/L$ 이었으며, 우기시는 $195{\pm}11\;Bq/L,\;45.4{\mu}g/L$ 이었다.

• Microbiology and Biotechnology Letters
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• v.24 no.4
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• pp.507-511
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• 1996

Medium optimization for xylitol production from xylose by Candida parapsilosis ATCC 22019 mutant was performed. Effect of various nitrogen sources on xylitol production was investigatied. Of inorganic nitrogenous compounds, ammonium sulfate was effective for xylitol production and yeast extract was the most suitable orangic nitrogen nutrient for enhancement of xylitol production. Effect of inorganic salts such as KH$_{2}$PO$_{4}$ and MgSO$_{4}$-7H$_{2}$0 on xylitol production was also studied. Optimal medium was selected as xylose of 50 g/l, yeast extract of 5 g/l, (NH4$_{4}$)$_{2}$SO$_{4}$ of 5 g/l, KH$_{2}$PO$_{4}$ of 5 g/l, MgSO$_{4}$-7H$_{2}$O of 0.2 g/l. In a fermentor by using the optimal medium, a final xylitol concentration of 37 g/l could be obtained from 50 g/l of xylose with a xylitol yield of 74% and a xylitol productivity of 0.58 g/1-hr. At 300 g/l xylose, fermentation was also carried out and then a final xylitol concentration of 242 g/l was obtained at 272 hours. It was corresponding to xylitol yield of 80.7% and xylitol productivity of 0.58 g/1-hr.