• The Korean Journal of Physiology and Pharmacology
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• v.12 no.3
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• pp.101-109
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• 2008

The aim of the present study was to examine the effects of ketamine, a dissociative anesthetics, on secretion of catecholamines (CA) secretion evoked by cholinergic stimulation from the perfused model of the isolated rat adrenal gland, and to establish its mechanism of action, and to compare ketamine effect with that of thiopental sodium, which is one of intravenous barbiturate anesthetics. Ketamine ($30{\sim}300{\mu}M$), perfused into an adrenal vein for 60 min, dose- and time-dependently inhibited the CA secretory responses evoked by ACh (5.32 mM), high $K^+$ (a direct membrane-depolarizer, 56 mM), DMPP (a selective neuronal nicotinic NN receptor agonist, $100{\mu}M$) and McN-A-343 (a selective muscarinic M1 receptor agonist, $100{\mu}M$). Also, in the presence of ketamine ($100{\mu}M$), the CA secretory responses evoked by veratridine (a voltage-dependent $Na^+$ channel activator, $100{\mu}M$), Bay-K-8644 (an L-type dihydropyridine $Ca^{2+}$ channel activator, $10{\mu}M$), and cyclopiazonic acid (a cytoplasmic $Ca^{2+}$-ATPase inhibitor, $10{\mu}M$) were significantly reduced, respectively. Interestingly, thiopental sodium ($100{\mu}M$) also caused the inhibitory effects on the CA secretory responses evoked by ACh, high $K^+$, DMPP, McN-A-343, veratridine, Bay-K-8644, and cyclopiazonic acid. Collectively, these experimental results demonstrate that ketamine inhibits the CA secretion evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors and the membrane depolarization from the isolated perfused rat adrenal gland. It seems likely that the inhibitory effect of ketamine is mediated by blocking the influx of both $Ca^{2+}$ and $Na^+$ through voltage-dependent $Ca^{2+}$ and $Na^+$ channels into the rat adrenal medullary chromaffin cells as well as by inhibiting $Ca^{2+}$ release from the cytoplasmic calcium store, which are relevant to the blockade of cholinergic receptors. It is also thought that, on the basis of concentrations, ketamine causes similar inhibitory effect with thiopental in the CA secretion from the perfused rat adrenal medulla.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.1
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• pp.146-170
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• 2017

Joint channel assignment and routing is a well-known problem in multi-radio wireless mesh networks for which optimal configurations is required to optimize the overall throughput and fairness. However, other objectives need to be considered in order to provide a high quality service to network users when it deployed with high traffic dynamic. In this paper, we propose a re-configuration optimization model that optimizes the network throughput in addition to reducing the disruption to the mesh clients' traffic due to the re-configuration process. In this multi-objective optimization model, four objective functions are proposed to be minimized namely maximum link-channel utilization, network average contention, channel re-assignment cost, and re-routing cost. The latter two objectives focus on reducing the re-configuration overhead. This is to reduce the amount of disrupted traffic due to the channel switching and path re-routing resulted from applying the new configuration. In order to adapt to traffic dynamics in the network which might be caused by many factors i.e. users' mobility, a centralized heuristic re-configuration algorithm called State-Aware Joint Routing and Channel Assignment (SA-JRCA) is proposed in this research based on our re-configuration model. The proposed algorithm re-assigns channels to radios and re-configures flows' routes with aim of achieving a tradeoff between maximizing the network throughput and minimizing the re-configuration overhead. The ns-2 simulator is used as simulation tool and various metrics are evaluated. These metrics include channel-link utilization, channel re-assignment cost, re-routing cost, throughput, and delay. Simulation results show the good performance of SA-JRCA in term of packet delivery ratio, aggregated throughput and re-configuration overhead. It also shows higher stability to the traffic variation in comparison with other compared algorithms which suffer from performance degradation when high traffic dynamics is applied.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.6
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• pp.630-635
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• 2014

The purpose of this study was to investigate the effects of Naeso-san in interstitial cells of Cajal (ICCs) in murine small intestine. First, we isolated ICCs from murine small intestine. After that, we cultured these cells for 1 days. The patch-clamp technique was applied on ICCs that formed network-like structures in culture (1 days). Spontaneous rhythms were routinely recorded from cultured ICCs under current-clamp conditions, and the ICCs within networks displayed more robust electrical rhythms (pacemaker potentials). To understand the relationship between Naeso-san and pacemaker activity in ICCs, we examined the effects of Naeso-san on pacemaker potentials of ICCs. In current clamp mode (I = 0), the addition of Naeso-san (10 mg/ml - 50 mg/ml) decreased the amplitude and frequency of the pacemaker potentials of ICCs in a dose dependent manner. However, these effects were blocked by intracellular $GDP{\beta}S$, a G-protein inhibitor, and glibenclamide, a specific ATP-sensitive K+ channels blocker. Pretreatment with SQ-22536, an adenylate cyclase inhibitor, did not block the Naeso-san induced effects, whereas pretreatment with ODQ, a guanylate cyclase inhibitor, or L-NAME, an inhibitor of nitric oxide (NO) synthase blocked the Naeso-san induced effects. Our findings provide insight into unraveling the modulation of Naeso-san in pacemaker potentials of ICCs and developing therapeutic agents against gastrointestinal motility disorders.

• Membrane Journal
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• v.30 no.3
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• pp.181-189
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• 2020

In the present study, a biomimetic alumina membrane was fabricated by using selenic acid as an electrolyte to overcome the asymmetry limit of the square pulse anodization process. The prepared membrane has conical channels with a minimum diameter of 10 nm, a maximum diameter of 50 nm, and a length of 5 ㎛. The rectification property was higher than membranes fabricated by sulfuric acid. It showed 2.9 times larger current at +1 V than -1 V. Also, the membrane, which sulfonic acid group was introduced by surface modification, showed 4.2 times larger rectification property at -1 V than +1 V. Theoretical verifications were supported by the numerical analyses of 2D models. The results of the present study present a convenient method to fabricate two type membranes with different rectification properties and are expected to be used to control ion transport.

• Korean Journal of Digital Imaging in Medicine
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• v.16 no.1
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• pp.43-47
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• 2014

Sens of insecutity of the public and professionals about harmful effects of radiation is increasing in an accident at the Chernobyl and Fkushima nuclear power plant.Anxiety was amplified to lack of information about radiation majority of people. To target the middle and high school in the region of Daegu and Gyeongsangbuk-do, to investigate the radiation recognition of the next generation, it is intended to present a model of education for the safe use of radiation. The High School of the six metropolitan cities, city, town through the questionnaire and needs to be educational experience of radiation and use knowledge level of radiation, experience in daily life, understanding of man-made radiation and natural radiation, information channel on radiation, the radiation Distribute the total 800 parts of, to recover the 629 unit, was analyzed for 155 females 474 males. Many people 75.36% of the people, to 24.64% female subjects of this investigation, was constant, respectively from 13 to 18 years age. It is a large number and 30.37% of the respondents as "normal" level of knowledge of radiation, for the type of radiation, most knew. You have answer for risk experience of the medical radiation was higher, touching a lot of information via the broadcast medium in general, and the accuracy is low. I thought we wanted to be educated three or more twice a year, as an educator,about 71.37% and radiation-related understanding of knowledge and background in accordance with the diversification of information channels, the regional differences between urban and rural areas. But I considered the difference age (grade) for each is displayed, intended for junior high school students, the target surface and use the occurrence of radiation, high school students, the need for education about risk and application of radiation through this study.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.5
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• pp.516-523
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• 2005

This study was performed to investigate dietary behaviors and consumption of health food in cancer patients. The subjects were 163 cancer patients recruited from the general hospital in Seoul, Korea. The data were obtained by the structured self administered questionnaire. The mean age of subjects was 51 years. Most of subjects didn't eat the visual fat of meat and chicken skin. The mealtime of the subjects was generally regular and most of subjects had breakfast. The subjects of this survey liked to eat foods with sweet taste but disliked to eat hot, salty and sour taste ones. The preference for fish, marine products and vegetables was high but that for instant foods and frying foods was very low. Most of subjects consumed the foods that is generally known as anticancer foods such as vegetables and fruits. On the other hand, most of subjects didn't intake butter, margarine, and frying foods that is known as risk factors of cancer. The majority of subjects$(84.7\%)$ consumed the health food The main reasons for taking health foods by subjects were to cure disease$(58.0\%)$, to prevent disease$(45.3\%)$, to supply nutrients$(39.3\%)$, to maintain the mental state$(12.7\%)$ and to recover fatigue$(10.7\%)$. Majority of subjects$(66.0\%)$ spent money more than 200,000 won/month to buy health foods. The purchasing channels of health foods by subjects were recommendation by family or friend$(64.0\%)$, by physician/pharmacist$(18.0\%)$ and by nutritionist or dietician(6.0), and advertisement through TV or radio$(12.0\%)$. The types of taking health foods of the subjects were vegetable extracted food$(60.0\%)$, mushrooms$(51.3\%)$, Lactobacillus food$(25.3\%)$, enzyme food$(22.6\%)$ calcium containing food$(20.0\%)$ and so on. The results of the current study show that although many cancer patients already practice healthy dietary behaviors, there is a substantial proportion who do not and most of cancer patients consume health foods.' Further intervention is needed to explore the effect of health foods in cancer patients

• The Korean Journal of Physiology and Pharmacology
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• v.4 no.1
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• pp.33-40
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• 2000

This study was designed to clarify the mechanism of the inhibitory action of a nitric oxide (NO) donor, 3-morpholino-sydnonimine (SIN-1), on contraction, cytosolic $Ca^{2+}$ level $([Ca^{2+}]_i)$ and ionic currents in guinea-pig ileum. SIN-1 $(0.01{\sim}100\;{\mu}M)$ inhibited 25 mM KCl- or histamine $(10\;{\mu}M)-induced$ contraction in a concentration-dependent manner. SIN-1 reduced both the 25 mM KCl- and the histamine-stimulated increases in muscle tension in parallel with decreased $[Ca^{2+}]_i.$ Using the patch clamp technique with a holding potential of -60 mV, SIN-1 $(10\;{\mu}M)$ decreased peak Ba currents $(I_{Ba})$ by $30.9{\pm}5.4%$ (n=6) when voltage was stepped from -60 mV to +10 mV and this effect was blocked by ODQ $(1\;{\mu}M),$ a soluble guanylyl cyclase inhibitor. Cu/Zn SOD (100 U/ml), the free radical scavenger, had little effect on basal $I_{Ba},$ and SIN-1 $(10\;{\mu}M)$ inhibited peak $I_{Ba}$ by $32.4{\pm}5.8%$ (n=5) in the presence of Cu/Zn SOD. In a cell clamped at a holding-potential of -40 mV, application of $10\;{\mu}M$ histamine induced an inward current. The histamine-induced inward current was markedly and reversibly inhibited by $10\;{\mu}M$ SIN-1, and this effect was abolished by ODQ $(1\;{\mu}M).$ In addition, SIN-1 markedly increased the depolarization-activated outward $K^+$ currents in the all potential ranges. We concluded that SIN-1 inhibits smooth muscle contraction mainly by decreasing $[Ca^{2+}]_i$ resulted from the inhibition of L-type $Ca^{2+}$ channels and the inhibition of nonselective cation currents and/or by the activation of $K^+$ currents via a cGMP-dependent pathway.

• Journal of Korean Society of Rural Planning
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• v.26 no.2
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• pp.107-116
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• 2020

Up to date, the majority research on the major landscape elements in organic farming has been mainly focused on the practice of seeking efficiency. The problem is that this type of study contributes to polluting the agricultural environment and damaging the ecological circulation system. As an alternative, there is a growing body of research on organic farming, but it is not widely applied that research on how to manage the landscape considering the scenic characteristics of farming villages practicing organic farming. Therefore, this study was carried out in the conservative aspects of rural landscapes in order to effectively manage the landscape of organic agriculture and, intended to be used to maintain and preserve natural and ecologically harmonious landscapes by deriving management methods suitable for landscape elements targeting the major landscape elements of the organic farming complex. To carry out, this study performed the experts survey which is composed of 13 major landscape elements, including rice paddies and fields, monoculture and diverse crops, dirt roads, windbreak trees, accent planting, dum-bung(small pond), natural small river, natural waterways, plastic film houses, one-storied houses, and pavilion. As a result, Farm land was formed in a square shape, concentrated in an independent space, planted companion plants around the crop, and covered with plants to manage the borders. As for the surrounding environment, it was analyzed that the aspart road system circulating through the village, the evergreen broad-leaved windbreak forest around the cultivated land, and the accent plant located at the entrance of the village were suitable. The hydrological environment consists of Round small pond made of stone in an open space, natural rivers around the village, and natural channels around the farmland, and The Major facilities are suitable for greenhouses that are shielded by plants in independent regions, and wooden duck houses located inside the cultivation area are suitable and The settlement facilities were analyzed to be suitable for single-story brick houses located in independent residential areas, pavilion located with greenery in the center of the village, and educational spaces shielded with wood from arable land. If supplementary evaluation criteria suitable for the management of organic farming landscape are additionally supplemented based on the results derived from this study, It is expected to enhance the landscape value of ecologically superior organic farming.

• Molecules and Cells
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• v.43 no.1
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• pp.66-75
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• 2020

Saturated fatty acids contribute to β-cell dysfunction in the onset of type 2 diabetes mellitus. Cellular responses to lipotoxicity include oxidative stress, endoplasmic reticulum (ER) stress, and blockage of autophagy. Palmitate induces ER Ca²⁺ depletion followed by notable store-operated Ca²⁺ entry. Subsequent elevation of cytosolic Ca²⁺ can activate undesirable signaling pathways culminating in cell death. Mitochondrial Ca²⁺ uniporter (MCU) is the major route for Ca²⁺ uptake into the matrix and couples metabolism with insulin secretion. However, it has been unclear whether mitochondrial Ca²⁺ uptake plays a protective role or contributes to lipotoxicity. Here, we observed palmitate upregulated MCU protein expression in a mouse clonal β-cell, MIN6, under normal glucose, but not high glucose medium. Palmitate elevated baseline cytosolic Ca²⁺ concentration ([Ca²⁺]_i) and reduced depolarization-triggered Ca²⁺ influx likely due to the inactivation of voltage-gated Ca²⁺ channels (VGCCs). Targeted reduction of MCU expression using RNA interference abolished mitochondrial superoxide production but exacerbated palmitate-induced [Ca²⁺]_i overload. Consequently, MCU knockdown aggravated blockage of autophagic degradation. In contrast, co-treatment with verapamil, a VGCC inhibitor, prevented palmitate-induced basal [Ca²⁺]_i elevation and defective [Ca²⁺]_i transients. Extracellular Ca²⁺ chelation as well as VGCC inhibitors effectively rescued autophagy defects and cytotoxicity. These observations suggest enhanced mitochondrial Ca²⁺ uptake via MCU upregulation is a mechanism by which pancreatic β-cells are able to alleviate cytosolic Ca²⁺ overload and its detrimental consequences.

• Korean Journal of Ecology and Environment
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• v.47 no.spc
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• pp.66-73
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• 2014

In order to determine whether vegetation would be one of the factors for the selection of otter home range, vegetation structure and other potential factors were studied in Hwacheon, Korea. Thirteen sites, otter's activity found and not found, were investigated in North Han River and connected tributary streams of Hwacheon-gun. Three types of vegetation were classified by cluster analysis, which is short grass, tall grass and shrub type. Vegetation zone of each channel is composed of either one type, or mosaic of tall grass and shrub type. Short grass type is common in Lake Paro and upper North Han-river where water level is highly variable throughout a year. Therefore, annual species such as Persicaria nodosa, Fimbristylis dichotomam and Chenopodium ficifolium are the most dominant. Shrub type is common at the downstream sites of Jichon stream and along mainstream of North Han River down Lake Paro. A shrub species, Salix koreensis, is the most common. Tall grass type is dominant occupying the most vegetation zone of the tributary channels. Phragmites japonica is absolutely dominant. Due to its dense cover, a few plant species are co-existed. Otter activity was found in all three vegetation types and no marked activity was found at some sites of tall grass type. There is no difference in species composition and physiognomy between tall grass sites with and without otter activity, while it shows significant difference in fish availability between two groups. Overall we found that home range of otters in the region is along the mainstream and downstream of tributary streams with high fish availability in all vegetation types and in various human activity levels.