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http://dx.doi.org/10.17208/kjopp.2014.12.28.6.630

Inhibitory Effects of Naeso-san on Pacemaker Potentials in Interstitial Cells of Cajal of Murine Small Intestine  

Hong, Noo Ri (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Ahn, Tae Seok (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Park, Hyun Soo (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Chae, Han (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Kwon, Young Kyu (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Kim, Byung Joo (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.6, 2014 , pp. 630-635 More about this Journal
Abstract
The purpose of this study was to investigate the effects of Naeso-san in interstitial cells of Cajal (ICCs) in murine small intestine. First, we isolated ICCs from murine small intestine. After that, we cultured these cells for 1 days. The patch-clamp technique was applied on ICCs that formed network-like structures in culture (1 days). Spontaneous rhythms were routinely recorded from cultured ICCs under current-clamp conditions, and the ICCs within networks displayed more robust electrical rhythms (pacemaker potentials). To understand the relationship between Naeso-san and pacemaker activity in ICCs, we examined the effects of Naeso-san on pacemaker potentials of ICCs. In current clamp mode (I = 0), the addition of Naeso-san (10 mg/ml - 50 mg/ml) decreased the amplitude and frequency of the pacemaker potentials of ICCs in a dose dependent manner. However, these effects were blocked by intracellular $GDP{\beta}S$, a G-protein inhibitor, and glibenclamide, a specific ATP-sensitive K+ channels blocker. Pretreatment with SQ-22536, an adenylate cyclase inhibitor, did not block the Naeso-san induced effects, whereas pretreatment with ODQ, a guanylate cyclase inhibitor, or L-NAME, an inhibitor of nitric oxide (NO) synthase blocked the Naeso-san induced effects. Our findings provide insight into unraveling the modulation of Naeso-san in pacemaker potentials of ICCs and developing therapeutic agents against gastrointestinal motility disorders.
Keywords
Naeso-san; interstitial cells of Cajal; patch-clamp technique; gastrointestinal motility;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Jun, J.Y. Interstitial Cells of Cajal. Korean J Gastrointest Motil. 7: 70-72, 2001.
2 Park, K.J. Interstitial Cells of Cajal and GI Motility. J Neurogastroenterol Motil. 10: 93-99, 2009.
3 Huizinga, J.D., Thuneberg, L., Kluppel, M., Malysz, H.B. The W/kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity. Nature 373: 347-349, 1995.   DOI   ScienceOn
4 Sanders, K.M., Tamas, O., Kho, S.D., Torihashi, S., Ward, S.M. Deveolpment of interstitial cells of cajal. Neurogastrointestinal motility 11: 3-11, 1999.
5 Kim, J., Yoon, S. Effect of Naesosan on Gastric Motility between Normal Intact and Antral Dilatated Rats. Korean J. Orient. Int. Med. 29: 117-129, 2008.
6 Jain, D., Moussa, K., Tandon, M., Culpepper-Morgan, J., Proctor, D.D. Role of interstitial cells of Cajal in motility disorders of the bowel. Am J Gastroenterol 98: 618-624, 2003.   DOI   ScienceOn
7 Kenny, S.E., Connell, M.G., Rintala, R.J., Vaillant, C., Edgar, D.H., Lloyd, D.A. Abnormal colonic interstitial cells of Cajal in children with anorectal malformations. J Pediatr Surg 33: 130-132, 1998.   DOI   ScienceOn
8 Hong, K.C., Ryu, B.H., Jang, I.K., Park, D.W., Ryu, K.W. A Study on the Effects Naesosan and Gaminaesosan on Secretion of Gastric Juice and Gastric Ulcer Rats. The Journal of Kyung Hee University Medical Center 6(3):313-320, 1990.
9 Park, S., Choi, J. Experimental study of Naesosan on the Effects of Anti-cancer. The Journal of Oriental Medical Surgery, Ophthalomology & Otolaryngology 14(1):154-166, 2001.
10 Kim, B.J., Lim, H.H., Yang, D.K., Jun, J.Y., Chang, I.Y., Park, C.S., So, I., Stanfield, P.R., Kim, K.W. Melastatin-type transient receptor potential channel 7 is required for intestinal pacemaking activity. Gastroenterology 129(5):1504-1517, 2005.   DOI   ScienceOn
11 Sanders, K.M. A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract. Gastroenterolgy 111: 492-515, 1996.   DOI   ScienceOn
12 Lee, H.K., Sanders, K.M. Comparison of the electric currents from interstitial cells and smooth muscle cells of canine proximal colon. J Physiol 460: 135-152, 1993.   DOI
13 Kho, S.D., Sanders, K.M., Ward, S.M. Spontaneous electrical rhythmicity in cultured interstitial cells of Cajal from the murine small intestine. J Physiol 513: 203-213, 1998.   DOI   ScienceOn
14 Ward, S.M., Ordog, T., Koh, S.D., Baker, S.A., Jun, J.Y., Amberg, G., Monaghan, K., Sanders, K.M. Pacemaking in interstitial cells of Cajal depends upon calcium handling by endoplasmic reticulum and mitochondria. J Physiol 525: 355-361, 2000.   DOI   ScienceOn
15 Flynn, E.R., McManus, C.A., Bradley, K.K., Koh, S.D., Hegarty, T.M., Horowitz, B., Sanders, K.M. Inward rectifier potassium conductance regulates membrane potential of canine colonic smooth muscle. J Physiol 518: 247-256, 1999.   DOI   ScienceOn
16 Zhu, M.H., Kim, T.W., Ro, S., Yan, W., Ward, S.M., Koh, S.D., Sanders, K.M. A Ca2+-activated Cl-conductance in interstitial cells of Cajal linked to slow wave currents and pacemaker activity. J Physiol 587: 4905-4918, 2009.   DOI   ScienceOn
17 Huizinga, J.D., Thuneberg, L., Kluppel, M., Malysz, J., Mikkelsen, H.B., Bernstein, A. W/kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity. Nature 373: 347-349, 1995.   DOI   ScienceOn
18 Sanders, K.M., Zhu, M.H., Britton, F., Koh, S.D., Ward, S.M. Anoctamins and gastrointestinal smooth muscle excitability. Exp Physiol 97: 200-206, 2012.   DOI
19 Kim, J.N., Kwon, Y.K., Kim, B.J. Effects of Samchulkunbi-tang in Cultured Interstitial Cells of Cajal of Murine Small Intestine. Korean J Oriental Physiology & Pathology 27: 112-117, 2013.
20 Hwang, M.W., Kim, J.N., Song, H.J., Lim, B., Kwon, Y.K., Kim, B.J. Effects of Lizhong Tang on cultured mouse small intestine interstitial cells of Cajal. World J Gastroenterol 19: 2249-2255, 2013.   DOI
21 Hwang, M.W., Lee, H.J., Song, H.J., Kim, B.J. Involvement of MAPKs and PLC pathways in modulation of pacemaking activity by So-Cheong-Ryong-Tang in interstitial cells of Cajal from murine small intestine. Scientific World Journal 2013: 536350, 2013.
22 Kim, B.J., Kim, H., Lee, G.S., So, I., Kim, S.J. Effects of San-Huang-Xie-Xin-tang, a traditional Chinese prescription for clearing away heat and toxin, on the pacemaker activities of interstitial cells of Cajal from the murine small intestine. J Ethnopharmacol 155: 744-752, 2014.   DOI
23 Nakahara, M., Isozaki, K., Hirota, S., Vanderwinden, J.M., Takakura, R., Kinoshita, K., Miyagawa, J., Chen, H., Miyazaki, Y., Kiyohara, T., Shinomura, Y., Matsuzawa, Y. Deficiency of KIT-positive cells in the colon of patients with diabetes mellitus. J Gastroenterol Hepatol 17: 666-670, 2002.   DOI   ScienceOn
24 Kim, J., Hwang, C., Lim, K. A Study on the Anti-inflammatory, Analgesic and Anti-bacterial Activity of Hwanhonsan Water extract and 20% Ethanol extract. The Journal of Wonkwang Oriental medicine 4(1):361-378, 1994.
25 Malysz, J., Huizinga, J.D. Searching for intrinsic properties and functions of interstitial cells of Cajal. Current Opinion in Gastroenterology 15: 26-32, 1999.   DOI   ScienceOn
26 Nakayama, S., Ohya, S., Liu, H.N., Watanabe, T., Furuzono, S., Wang, J., Nishizawa, Y., Aoyama, M., Murase, N., Matsubara, T., Ito, Y., Imaizumi, Y., Kajioka, S. Sulphonylurea receptors differently modulate ICC pacemaker Ca2+ activity and smooth muscle contractility. J Cell Sci 118: 4163-4173, 2005.   DOI   ScienceOn