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수정에 실패한 인간 난자에 있어서의 염색체의 수의 이상 (Chromosomal Abnormalities in Human Oocytes Fail to Fertilize after Insemination In Vitro)

  • 손원영;이경아;박상희;한세열;윤태기;정형민;곽인평;차광열
    • Clinical and Experimental Reproductive Medicine
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    • 제22권2호
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    • pp.203-210
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    • 1995
  • Many oocytes fail to fertilize and cleave in vitro and many embryos transferred back to uterus fail to implant or maintain implantation. Chromosomal abnormalities in the male and female gametes may contribute to this loss. The higher incidence of meiotic chromosomal abnormalities bas been found in oocytes than in sperm. The wide range of incidence of chromosomal abnormalities in unfertilized oocytes has been reported in human IVF program (26-63%). However, factors affecting chromosomal abnormalities are not well understood. The present study has been conducted to investigate effects of the method for ovarian hyperstimulation, women's age, and the number of oocytes retrieved per patients on the incidence of numerical chromosomal abnormalities. Five hundred eighty four unfertilized metaphase II oocytes were subjected to chromosomal analysis. Included unfertilized oocytes were from 220 patients (mean $age=32.7{\pm}3.0$) and three hundred thirty oocytes were legible for analysis. Two hundred fourty five oocytes out of 330 (73.3%) were normal, while 38 (11.5%) were hyperploidy, 35 (10.6%) were hypoploidy, and 12 (3.6%) were diploidy. Significant difference in chromosomal abnormalities was not found between two patient groups stimulated by follicular stimulating hormone/human menopausal gonadotrophin (FSH/HMG) (25.9%) and gonadotrophin-releasing hormone agonist/follicular stimulating hormone/human menopausal gonadotrophin (GnRHa/FSH/HMG) (28%). There was a tendency of increasing chromosomal abnormalities in unfertilized oocytes from older patients (<30 yrs: 20.3%, 30-34yrs: 26.9%, >34 yrs: 35.3%). The number of oocytes retrieved per patient had no effect the incidence of chromosomal abnormalities (1-5: 31. 4%, 6-10: 29.8%, 11-15: 28.6%, > 15: 16.5%). These results from the present study suggest that the chromosomal abnormalities observed in the unfertilized oocytes has not affected by the stimulation methods, patient's age, and the number of oocytes retrieved per patients.

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가정관리실습관에 있어서의 학생지도에 관한 연구 - 서울 시내모여자대학교대상- (A Study on the Student Guidance at Home Management House)

  • 김복길
    • 대한가정학회지
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    • 제12권34호
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    • pp.679-697
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    • 1974
  • I. Preface A. Establishment. There are many psycological and physiological difficulties that students have made practical application to a building site of 600 pyung and a structure of 65 pyung each sixteen students in the Home Management House, but there exists problem in students apprentice and management of professors. B. Purpose of study This research aim to seek a high degree of efficiency approach in investigating, studying and analyzing factors of the problem in Home Management which have been faced in women colleges in seoul. C. Content of study. This study is aim at finding out the problems including facilites, system, guiding principle, working bottleneck and administrative problem in making practical application of Home Management. II. A plan and method of study A. This subject of statiscal research is 616 women students in senior year from september 27 1970 to October 31 1974. The rate of collection of questionnaire is 100 percent. B. The Method of study is devised through refering documents, interview with students, questionnaire and observation. III. Result of study Of the total of 29 university in seoul, 24 Universities have its Course of Home Economic among which only 12 have Home Management for making practical application. Home Management House for making practical application is located mainly in campus but some of them exceptimally are in outside university. Students practice mainly at civilzed resident, apartment, bangalow so forth, and demention of which occupies approxiamtely 50-600 pyung (land) and 20-70 pyung (Building). A member with 4 or 20 students make practical application from 10 days to 3 months. A member consists of students and assistant teacher, and practice site classifies into living school for Professors living, and another school for Professors and assistant teachers. Answer to a questionnaire. 616 students answer to a questionnaire. 49 Perfect of 616 students like to have a member with 12 students like to have a member with 12 students, 38 percent student a member with 16 students. About the period of making practical About the period of making practical application, 76 percent students like to have two or three week period. 19 percent students think it a short period and 4 percent students think it a long period. There is none who thinks 25 day period day period to be short. About making practical application, 66 percent of 616 students feel uneasy, 40 percent students feel lack of sleep and tired. Students take a pleasure in having a conversation with their friends, inviting their mother, having dinner-party and birthday party. 77 percent students want to invite their father and 67 percent students want to invite their boy-friends. It is hard to have a good personal relation about the great the result of practice, students acknowledge themselves that they have learned about Cautiousness and they are sure of good Home Manager. There are 9 difficult problems during the period. IV. Summary discussion a. Many problems come from having a number of students. b. Students feel tired with their practice if over three weeks. No Matter how good a plan is, it useless if students can't adapt themselves to it. Suggestions 1) it is good to have a member with 5 or 6 students, and it is suitable to have two or three week period, and to have an area of 20 or 30 pyung. 2) it's proposed a profess or to live together in making practical application of Home Management 3) it is proposed a professor to have a sure T.O. and to be treated better.

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공황장애 환자의 성격특성과 임상적 의의 (Personality Trait in Panic Disorder Patient and Its Clinical Significance)

  • 채영래;이정태;김보연;이성필;홍승철;김종우;계윤정
    • 정신신체의학
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    • 제3권2호
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    • pp.139-146
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    • 1995
  • 공황장애 환자에서 동반되는 인격장애의 양상을 조사하고, 인격장애 특성의 과다(過多)에 따른 공황증상의 심각도, 만성도, 전반적인 정신병리의 차이를 알아보고자 자기보고형 측정도구인 Personality Disorder Questionnaire-Revised(PDQ-R)와 간이 정신상태검사(SCL-90-R)를 이용하여 조사하였다. 대상은 전형적인 공황장애 환자 45명과 성별. 나이, 교육정도를 조합한 건강대조군 45명이었으며 이들에게 PDQ-R을 실시하여 공황장애 환자군과 대조군의 인격장애 양상을 비교 조사하였다. 또한 공황장애 환자만을 대상으로 공황증상의 심각도와 함께 SCL-90-R로 인격장애 동반여부에 따른 증상의 차이를 조사하여 다음과 같은 결과를 얻었다. 1) 공황장애 환자들은 대조군에 비하여 전체 PDQ-R점수가 유의하게 높게 나타나 차이가 있었다. 공황장애군은 대조군에 비하여 편집성, 의존성, 회피성 인격의 특징과 함께 반사회성, 경계성 경향을 나타내었다. 2) PDQ-R총점이 높은 집단과 낮은 집단 사이에 광장공포증상과 불안증상이 유의한 차이가 있었으나, 공황발작 빈도, 공황증상의 최근 심각도, 공포증상으로 인한 사회적 기능장애, 만성도 여부, 광장공포증 때 회피 및 공포증상의 정도에는 차이가 없었다. 3) SCL-90-R을 이용하여 동반증상을 알아본 결과 PDQ-R총점이 높은 군은 낮은 군에 비하여 모든 증상 항목이 의미 있게 높게 나타났다. 이러한 본 연구의 결과는 PDQ-R을 이용한 인격장애 측정시 동반증상이 영향을 미쳐 혼합되어 나타났을 가능성을 고려해야 하겠으며 앞으로 더욱 체계적인 조사방법과 함께 추적조사를 통하여 치료에 따른 인격장애의 변화를 관찰해야 하겠다.

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저소득층의 의료 이용과 욕구 미충족에 영향을 미치는 요인 (Factors Affecting the Choice of Medical Care Use by the Poor)

  • 김진구
    • 사회복지연구
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    • 제37호
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    • pp.5-33
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    • 2008
  • 본 연구는 국민건강영양조사 3기 자료를 이용하여 저소득층의 의료이용에 대해 살펴 보았으며, 분석의 초점은 충족되지 않은 의료욕구와 의료급여제도의 효과에 두어졌다. 분석 결과 전반적으로 저소득층은 건강상태가 좋지 않은 반면 가구총소득이나 직업 등 가능요인 측면에서는 매우 불리한 조건에 놓여 있어, 의료욕구는 높으나 이를 충족시킬 자원이 부족한 상황인 것으로 나타났다. 그럼에도 불구하고 실제 저소득층의 의료기관 이용여부는 입원, 외래, 약국 등 모든 측면에서 일반인들보다 높게 나타났으며, 이는 다른 요인들을 통제했을 경우에도 유의미하였다. 분산분석(ANOVA) 결과 이용횟수에 있어서도 의료급여수급자들이 많았다. 반면, 비용 측면에서는 분산분석에서 통계적인 유의도가 검증되지 않았으나, 질병관련 요인을 비롯한 다른 요인들을 통제했을 경우 의료급여제도는 분명히 의료비용을 낮추는 효과가 있다. 의료이용에 대한 분석에서 의료급여제도는 저소득층의 좋지 않은 건강상태와 빈약한 경제적 조건을 효과적으로 보완하여 긍정적으로 기능하고 있는 것으로 분석되었다. 그러나, 의료급여제도는 저소득층의 의료욕구를 충분히 충족시켜주는데는 여전히 부족한 것으로 나타났다. 저소득층의 충족되지 않은 의료욕구는 여전히 일반인들보다 크며, 그 이유 중 가장 큰 것은 경제적인 이유였다. 이에 대한 계량분석결과 의료급여 1종과 2종, 차상위계층 등 모든 빈곤계층은 의료이용 포기가 일반 건강보험 가입자에 비해 높은 것으로 제시되고 있다. 의료급여 대상자들의 높은 의료이용량에도 불구하고 여전히 저소득층의 충족되지 않은 의료욕구가 크다는 것은 의료급여가 대상자들의 자원부족을 보완하여 어느정도 의료접근성을 높여주고 있지만 대상자들이 가진 의료욕구를 완전히 해소하기에는 불충분하다는 것을 의미하는 것이다.

일본 해상자위대 전력건설 재조명을 통한 한국 해군력 건설논리 발전방향 연구: '8함(艦) 8기(機) 체제'를 중심으로 (A Study on Developing Korean Naval Forces by Examining How Japan built their Maritime Self-Defense Force: Focusing on the 'eight (艦)-eight (機) fleet system')

  • 류재학
    • 해양안보
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    • 제2권1호
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    • pp.43-70
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    • 2021
  • 이 연구는 일본은 부국(富國)이고 섬나라이기 때문에 해상자위대의 강력한 해상전력건설은 당연한 결과로 인식하고 있는 일반적인 우리의 시각에 대한 의문에서 출발하였다. 일본은 2차 대전 후 해군력이 완전히 와해되었다. 1954년 해군을 대신하여 탄생한 해상자위대 초기전력은 대부분 함정을 미국으로부터 공·대여로 획득하는 등 한국해군 창설기의 전력과 별반 차이가 없을 정도로 미미하였다. 물론, 세계 제2의 경제대국으로 성장과 도서국가의 지리적 특성은 해군력 신장에 기회 요인임에 분명하지만, 창설 초 일본해군과의 일시적 단절, 육상병력 중심의 전략적 사고, 석유파동에 따른 경제위기 등의 난관이 있기도 하였다. 결과적으로 현재 해상자위대는 세계 정상급의 해군력을 구축하였고 전수방위라는 기본방위 개념에 큰 변화를 줄 수 있는 4개 헬기호위형 항모운용 체제로 발전하려고 하고 있다. 일본 해군력의 성장 배경에는 다양한 요인이 있지만, 본 연구는 해상자위대가 해군력 건설을 위해 창출한 대표적인 건설논리인 '8함(艦) 8기(機) 체제'를 중심으로 해상자위대의 핵심전력인 호위함대 전력건설 과정을 분석하여, 한국해군력 건설논리 발전방향을 모색하고자 한다.

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미국 중산층 여성 일-가정양립 경로의 역사적 형성과정에 관한 연구 (The American Route of the Reconciliation between Work and Family)

  • 최성은;양재진
    • 한국사회정책
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    • 제23권3호
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    • pp.79-108
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    • 2016
  • '저복지 국가인 미국에서, 여성들의 일-가정양립은 어떠한 역사적 과정을 거쳐 이루어져 왔을까?' 이 질문에 답을 하고자, 미국 여성 일-가정양립경로의 형성과정에 대한 역사적 인과관계를 4단계시기로 나누어 살펴보았다. 과거 전업주부에 머물렀던 중산층 기혼여성을 대상으로 하여, 여성 일-가정 양립모델에 대한 역사적 제도주의적 접근을 시도함으로써 기존연구들과의 차별성을 두었다. 주요 연구결과는 다음과 같다. 제1단계는 여성의 고등교육이 확대되고, 산업변화로 미혼여성들의 사회진출이 시작된 시기였다. 제2단계는 중산층 여성들의 사회진출이 뉴딜, 소비자본주의, 제2차 세계대전을 통해 확대된 시기이다. 제3단계는 여성해방운동으로 정부의 적극적 조치에 대한 '적극적' 시행이 요구되어 전문화된 직업영역으로 여성의 일자리가 확대된 시기였다. 제4단계에서는 더 나은 정규직 일자리로 진입하려는 여성 근로자의 공급 측면과, 양질의 근로자를 얻고자 하는 사용자의 수요 부분이 개별고용관계를 통해 노동시장 제도 내에서 맞물렸다. 이로써 맞벌이의 규범화가 이루어졌다. 즉, 저복지국가인 미국에서는 여성의 개별적 노력(학업을 통한 능력향상과 여성운동)과 개별고용관계가 발달된 노동시장 특징으로 인하여, 고학력 중산층 여성의 '고(高)고용-고출산'이 가능하며, 정규직 일자리로의 재복귀가 비교적 용이한 여성 일-가정양립 패턴이 완성될 수 있었다.

고속철도 지방대도시 정차역의 연계교통수단 선택모형 구축에 관한 연구 - 동대구역을 사례로 - (Examining Access Mode Choice Behavior of Local Metropolitan High-Speed Rail Station - A Case Study of Dong-Daegu Station -)

  • 김상황;김갑수
    • 대한토목학회논문집
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    • 제26권4D호
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    • pp.565-571
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    • 2006
  • 경부고속철도가 2004년 4월에 개통되어 운행되고 있으며, 이로 인해 교통체계의 변화와 함께 그에 따른 광역교통 네트워크의 변화를 초래하고 있다. 하지만 고속철도가 개통되어 정착단계인 현시점에서 고속철도 이용수요가 당초 목표치의 60%정도밖에 되지 않아 경부고속철도의 도입효과에 의구심을 던지는 견해도 만만치 않다. 고속철도 이용수요가 기대치에 못 미치는 이유로 고속철도의 값비싼 운임 등을 들 수 있으나 그에 못지않게 연계교통체계의 중요성을 지적할 수 있다. 문전수송(door-to-door) 서비스를 갖춘 승용차와는 달리 고속철도와 같은 대중교통은 출발지에서 역까지의 혹은 역에서 목적지까지의 연계교통서비스에 따라 그 이용정도가 크게 달라질 수 있다. 이에 본 연구에서는 고속철도 시대에 부응하여 지방대도시 정차역, 특히 동대구역을 중심으로 연계교통시스템의 현황을 분석하고, 다항로짓모형을 이용한 고속철도 연계교통수단 선택모형을 구축하는 것이 목적이다. 고속철도 연게교통수단으로 버스, 지하철, 택시, 승용차, 도보 및 기타로 구분하였고, 모형의 추정결과는 모든 변수들의 파라메타 값이 논리적으로 합당한 부호를 나타내었다. 특히 접근통행시간, 접근통행비용 등이 모두 음(-)의 부호를 가지면서 통계적 유의성이 있는 것으로 나타나 접근통행시간과 접근통행비용이 연계교통수단 선택에 중요한 영향을 미치는 것으로 파악되었다.

광주지역 공공수역의 미생물 군집 다양성 및 항생제 내성에 관한 연구 (A Study on Microbial Community Diversity and Antibiotic Resistance in Public Waters in Gwangju)

  • 김선정;박지영;김승호;임민화;유지용;한규성;박세일;서광엽;조광운
    • 한국환경보건학회지
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    • 제50권2호
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    • pp.93-101
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    • 2024
  • Background: As pollutants caused by non-point sources flow into rivers, river water quality monitoring for fecal pollution is becoming increasingly important. Objectives: This study was conducted to investigate the distribution of microbial communities in the Yeongsangang River water system and sewage treatment plants in Gwangju and to evaluate their antibiotic resistance. Methods: In the experiment, samples were distributed to five selective media at each point and then cultured for 18 to 24 hours. When bacteria were observed, they were sub-cultured by size and shape and identified using MALDI-TOF MS equipment. When identification was completed, 17 types of antibiotic susceptibility tests were performed using VITEK II equipment, focusing on gram-negative dominant species among the identified strains. Results: During the study period, a total of 266 strains were isolated from 39 samples. Gram-positive bacteria were 37 strains in four genera, or 13.9% of the total, and Gram-negative bacteria were 229 strains in 23 genera, or 86.1% of the total. Antibiotic susceptibility testing of 23 strains, the major dominant species, showed that one strain (4.3%) was resistant to only one antibiotic, and two strains (8.7%) were 100% susceptible to the 17 antibiotics tested. The other 20 strains (87.0%) were multidrug resistant bacteria resistant to two or more antibiotics. There were various types of multidrug resistance. Among them, penicillin and cephalosporin series showed the highest resistance. Conclusions: Based on the results of this study, it was found that the bacterial community structure changed according to regional and environmental factors, and it was judged that continuous research such as genetic analysis of antibiotic-resistant bacteria present in natural rivers is necessary.

한국 청소년의 약물남용과 비행행위

  • 김성이
    • 한국인구학
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    • 제11권2호
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    • pp.54-66
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    • 1988
  • I. Introduction Since the 1970's drug abuse among young people has increasingly become a social problem in Korea. In the 1980's, drug abuse, especially glue sniffing, has become the cause of many unfortunated incidents resulting in harm to others as well as the abusers themselves. Taking into consideration of the seriousness of this problem, the Republic of Korea National Red Cross initiated a nation-wide research programme, to understand the present situation and to raise the level of public awareness. The goal of this research was to begin a nation - wide campaign against drug abuse. The research team was composed of the Advisary Committee members and the staff of the Youth Department of the Republic of Korea National Red Cross. The data were collected in February 1988 with the collaboration of the staff and volunteers in the local Chapters. The respondents were allocated nation-wide by the quota sampling method. The questionnaires were distributed to the respondents in three groups :2, 700 to junior and senior high school students, 605 to working youths, and 916 to delinquent youths. A total of 4, 221 questionnaires were collected. II. Characteristics of the Respondents The respondents in each group were selected evenly from rural and urban areas. The general characteristics of the respondents can be described as follow: in case of students, the proportions between male and female respondents, and between senior high school and junior high school students were almost evenly distributed. In case of working youths, the proportion of females (80.5%) was higher than those of the students and the delinquents groups. Delinquent youths were defined as those currently being under custody of the centers for juvenile delinquents. Of this number, 38.8% and 68.2% were junior and senior high school drop-outs respectively. The majority of them (92.6%) were male. As for the family background of the respondents, the proportion of those residing in poverty - stricken areas, and the proportion of those from broken families were higher in case of working youths and delinquent youths than those in case of students. III. Present Patterns of Drug Abuse The following summarizes the presents of drug abuse, as tabulated from the results of the survey. 1. Smoking The percentage of youths who smoke was 36% in the student group, 32% m the working youths group, and 94.4% in the delinquent youths group. 2. Alcohol 50.3% of students, 71.6% of working youths, and 93.3% of delinquent youths has experienced drinking alcohol beverages. 3. Tonic: non - alcoholic, caffeinated beverages popular in Korea and Japan The percentage of those who have used tonic at least once was over 90% in all of the three groups. 4. Sedative About 70% of each group has used sedative with the proportion of working youths use higher than those in other groups. 5. Stimulants Those who have used stimulants comprised around 15% in each group. 6. Tranquilizers Somewhat less than 5% of students and working youths, and 28% of delinquent youths, have used tranquilizers. 7. Hypnotics The users of hypnotics amounted to 0.4% of students, 2.6% of working youths and 7.1% of delinquent youths. 8. Marihuana Those who have used marihuana indicated 0.7% of students, 0.8% of working youths, and 13% of delinquent youths. 9. Glue-sniffing The percentage of glue-sniffing was 3.7%, 5% in the students group and in the youths group respectively, but the proportion was unusually high, at 40.7% in the delinquent youths group. From the results of the survey the present situation of drug abuse in Korea can be summarized as follows: 1. A high percentage of Korean youths have experienced smoking cigarettes and drinking alcoholic beverages. 2. Tonics (non - alcoholic, caffeinated beverages), antipyretic analgesics and stimulants quite regularly used. 3. Tranquilizers, hypnotics, marihuana and glue-sniffing are more widely used among delinquent youths than the other youths. From this fact, there exists a correlation between drug abuse and juvenile delinquency. IV. Time-series Analysis of the First Experience of Drug Abuse and Deviant Behaviour The respoundents were asked when they were first exposed to drugs and when they committed deviant acts. By calculating the average age of each experience, the following pattern was found (See Figure 1). Youths are first exposed to drugs by abuse of tonic(non - alcoholic, caffeinated beverages). At the age of 13, they amoke cigarettes, the use of antipyretic analgesics begins at 14 year old, while at the age of 15, they use tranquilizers, and at 16 hynotics. The period of drug abuse which starts from drinking caffeinated beverages and smoking cigarettes and ends in the use of hypnotics takes about three years. During this period, other delinquent behaviours begin to surface, that is, at the age of 13 when smoking cigarettes begins, the delinquent behaviour pattern starts with truancy. Next, they start taking money from others by using physical force. Prior to the age of 15, they are suspended from school, become hostile to adults, begin running away from home, and start using stimulants and alcohol. Soon they become involved even in glue-sniffing and in the use of marihuana. At the age of 15, they begin to see adult videos and carry weapons. Sexual promiscuity and usage of tranquilizers follows the viewing of adult videos. Consequently, by the time they reach the age of 16, they visit drinking establishments, and are picked up by police for committing delinquent acts. And finally, they come to use hypnotic - type drugs. From the above descriptions, drug abuse can be assumed to have a close correlation with delinquent behaviour. V. Social Factors Related to Drug Abuse As for the Korean youths, glue-sniffing is found to he related to aggressive delinquency, in such cases as run - aways, being picked up by the police, and taking money by force. Smoking cigarettes and drinking alcohol is found to be related to seeing adult videos and visiting drinking establishments. Hypnotics and marihuana were found to be representive of drugs which are related to degenerational delinquency, irrespective of social delinquency. The social factors connected with these drug abuse are as follows: 1. Individual factors Male students were more heavily involved in the usage of drug than females. Youths who do not attend church were more likely to be involved in drugs than those who attend. 2. Family factors The youths who were displeased with their mothers smoking and those who thought their parents did not love each other, or those whose parents had used drugs without prescription, were more likely to he drug users. 3. School factors Those youths who found school life boring, were unsuccessful in their studies, spend most of their time with friends, feel their teachers smoke too much, those who had a positive perception of their teachers smoking were likely to he drug users. To sum up, drug abusers depend on the influence of their parents, teachers and peers. IV. Reasons for Drug Abuse Korean students have mainly used drugs to release stress (42.8%), to stay awake (19.7%), and because of the easy accessibility of drugs( 16.6%). Other reasons are due to their ignorance of the side effects of the drugs (3.6%), natural curiosity (4.2%), and to increase strength(3.O%). From the above facts, the major reasons for drug abuse among Korean youths are to release stress and to stay awake in order to prepare exams. Furthermore, since drugs are readily available, we can conclude that drug abuse is caused by the school system(such as entrance exams) in Korea. VII. Conclusion Drug usage among Korean youths are relatively less common than those of western youths. In some cases, such as, glue-sniffing and use of stimulants, the pattern of drug abuse is found. Moreover, early drug abuse is evident, and it has a close connection with deviant behaviour, resulting in juvenile delinquency. Drug abuse cannot be attributed to any one social factor. Specifically, drug abuse depends on parents, peers, teachers and other members of the community, and also is influenced by social institutions such as the entrance exam system. Every person and organization concerned with youth must participate collectively in restraining drug abuse. Finally, it is suggested that social agencial working for youth welfare should make every effort to tackle this serious problem confronted by the Korean youths today.

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생쥐 배아 줄기세포의 Buforin 2 및 pEP-1에 결합된 EGFP의 세포 내 수송 (Mouse Embryonic Stem Cell Uptakes of Buforin 2 and pEP-1 Conjugated with EGFP)

  • 정수현;박성순;임현정;전용필
    • 한국발생생물학회지:발생과생식
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    • 제11권2호
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    • pp.111-119
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    • 2007
  • 배아줄기세포는 외부 기인 특정 요소를 이용한 세포 조절물질 활성을 통하여 원하는 세포형태로 분화될 수 있다. 배아줄기세포의 이용성세포침투성단백질(CPP)은 몇 개의 아미노산으로 구성된 작은 펩타이드로 유용한 물질 수송계 중의 하나로 인식되고 있다. 본 연구에서는 몇 종류의 CPP를 이용하여 특정 단백질을 생쥐배아줄기세포(R1)내로 트렌스펙션하는데 있어 그 정도를 결정할 수 있는 요소들의 영향을 분석하였다. CPP인 Buforin II, pEP-1을 강도를 강화한 녹색형광단백질(EGFP)에 부착되도록 하여 살아있는 세포에서 볼 수 있도록 하였다. 다양한 시간대별로 그리고 다양한 농도로 재조합 CPP-EGPF를 생쥐 배아줄기세포에 처리하였으며, 대조군으로 사용한 EGFP는 CPP를 갖고 있지 않았다. 재조합 CPP-EGFP들이 R1 배아줄기세포에 미치는 세포독성은 CPP 종류와 재조합 CPP-EGFP 농도에 의존적이었다. CPP-EGFP가 배아줄기세포의 세포질이나 핵질로 들어간 증거는 처리 후 30분 이내에서는 찾을 수 없었다. 1시간 후, pEP-1-EGFP-N을 처리한 일부 세포에서만 형광단백질이 세포안으로 들어간 것을 발견할 수 있었다. 처리를 24시간까지 하였으나 흥미롭게도 세포안으로 들어가는 정도와는 비례관계가 없었다. 또한, 배아줄기세포의 막을 통과할 수 있는 CPP의 경우, 처리 농도는 세포막을 침투하여 세포내로 이동하는 것과 비례적 관계는 없으나, 매우 높은 농도에서 약간 증가하는 경향을 보였다. 요약하면, CPP-EGFP의 트렌스펙션 효과는 전적으로 CPP에 의존적이며, CPP에 따라서는 His tag의 위치 또한 트렌스펙션에 영향을 줄 수 있으며, 처리시간은 CPP를 이용하여 배아줄기세포내로 단백질을 수송하는데 있어 중요 변수가 아니다. 또한, CPP-EGFP의 농도는 배아줄기세포막을 가로질러 수송되는데 큰 영향을 주지 않았다. 앞으로의 연구에서, 이들 CPP의 변형 또는 매개자 개발을 통한 배아줄기세포에서 좋은 단백질성 매개 수송자를 만드는 것이 필요하며 이를 이용한 분화 유도는 매우 유용할 것으로 사료된다.

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