• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.8 no.1
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• pp.65-70
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• 2010

The distillation rate on LiCl-KCl eutectic salt under different vacuums from 0.5-50 mmHg was first investigated by using both a non-isothermal and a isothermal thermogravimetric (TG) analysis. Based on the non-isothermal TG data, distillation rate equations as a function of the temperature could be derived. Calculated flux by these model flux equations was in agreement with the distillation rate obtained from isothermal TG analysis. A distillation rate of $10^{-4}-10^{-5}$ mole $cm^{-2}sec^{-1}$ is obtainable at temperatures less than 1300K and vacuums of 0.5-50 mmHg. About a 99% salt distillation efficiency was obtained after an hour at a temperature above 1150 K under 50 mmHg in a small scale distillation test system. An increase in the vaporizing surface area is relatively effective for removing residual salt in the remaining particles, when compared to that for the vaporizing time. Over 99.95% of total distillation efficiency was obtained for a 1-h distillation operation by increasing the inner surface area from $4.52cm^2$ to $12.56cm^2$.

• The Journal of Natural Sciences
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• v.10 no.1
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• pp.57-61
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• 1998

Postharvest dipping of vacuum infiltration treatments of 'Chojuro' pear fruit in $CaCl_2$ resulted in increased calcium content, especially in fruit peel and outer flesh such as just below peel. As $CaCl_2$ concentrations increaced from 2% to 8% in dipping treatment, calcium content became higher. But vacuum infiltration under 200-600 mmHg did not affect and dipping and vaccum infiltration did not have any difference in fruit calcium content. Decreasing of fruit firmness determined at 4 weeks during storage was slower in fruits with $CaCl_2$ treatment than control.

• Applied Microscopy
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• v.30 no.2
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• pp.153-163
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• 2000

This study aims to demonstrate the effect of squalene (SQ), one of the natural chelator, on the ultrastructural changes in the mouse liver caused by $HgCl_2$. A total of 40 healthy ICR that weighted 30 gm $({\pm}2gm)$ was used for experiment. The experimental group was divided into two groups; group A and B. The group A administrated $HgCl_2$ (4.0mg/kg) to the intraperitoneal. The group B administrated $HgCl_2$ (4.0 mg/kg) to the intraperitoneal treated with SQ (180 mg/kg, 2 times/day). Each group was observed at 24, 48, 72, 96 hours after injected $HgCl_2$. The results were as follows: 1. Group A Nucleus showed condensation of nuclear membrane at the 24 hours. At the 48 hours, observed distinct condensation. But nuclear membrane be seen relative rounded-shape at the 96 hours. At overall the time, inner cavity of mitochondria swollen and development of cristae weakened. Also electron density of matrix was a little low. At the 72 hours, destruction of the inner and outer membrane of mitochondria observed occasionally. Swelling of inner cavity of rER and destruction of lamellae be found from 24 hours to 72 hours, but at the 96 hours, only some swelling 2. Group B Nuclear membrnae and chromatin be seen normal shape at overall the time. Mitochondria showed destruction of the inner membrane until the 48 hours, but mostly normal shapes. Electron density showed high on the all groups. RER be found swelling of inner cavity at the 24 and 48 hours, but found typical lamellae and observed a number of transfer vesicles around rER at the 72 and 96 hours. These results suggest that squalene attenuates the toxic effect of the $HgCl_2$ in the mouse liver.

• Analytical Science and Technology
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• v.11 no.4
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• pp.297-304
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• 1998

The three analysis methods, EPA method 3050, the method offered by Ministry of Environment in Korea, and modified method corrected in this laboratory, were studied to investigate the effect of matrix on the analysis of inorganic priority pollutants. 7 inorganic priority pollutants(Ni, Cr, Cu, Zn, Pb, Cd, Hg) were spiked to the plating, leather, paper, electric, and dye sludges. Mean recovery of the elements except Hg was 95.5% when the procedure of EPA method was applied. However, recovery by the two other extraction methods showed 11.1% and 27.7%, respectively. Digestions were done by MDS (microwave digestion system) and $HNO_3+HClO_4$ methods. To study organic and inorganic matrix effect, samples were made by adding triethanol amine as a organic matrix and $FeCl_3{\cdot}6H_2O$+$AlCl_3{\cdot}6H_2O$ as a inorganic matrix, respectively. The extracts were analyzed by AAS and HG-AAS. Mean recovery of the elements by the $HNO_3+HClO_4$ procedure, except Hg, gave better result than that of the MDS method. Mean recovery of elements was decreased when organic and inorganic matrices were added in the sludge samples. The procedure of MDS and $HNO_3+HClO_4$ digestion gave higher recoveries than that of direct analysis. In general, the results of the studies showed a significant matrix effect on the inorganic priority pollutants analysis in sludges.

• Microbiology and Biotechnology Letters
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• v.27 no.4
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• pp.278-285
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• 1999

In order to use heat shock promoter for the detection of toxic substances, dnaK promoter was amplified from E. coli genomic DNA by using a polymerase chain reaction(PCR) followed by sequencing and sub-cloning into the multi-cloning site of the plasmid, pUCD615. The pUCD615 is a broad-host-range vector containing promoterless lux operon originated from V.fischeri. The recombinant plasmid was transfered to E. coli DH5$\alpha$ through electroporation. The recombinant E. coli showed several patterns of bioluminescent responses to ethanol stress. The bioluminescent E. coli also showed responses to other toxic substances including FeK3(CN)6, CdCl2, p-nitrophenol and HgCl2. The increases of RLU(Relative Light Unit) were observed at 100ppm of FeK3(CN)6, 10ppm and 100ppm and 100ppm of CdCl2, 1ppm of 10ppm of p-nitrophenol and at 1ppm of HgCl2.

• Journal of the Korean Chemical Society
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• v.36 no.4
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• pp.565-578
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• 1992

It has been suggested that Hg$^{2+}$-promoted reaction of a series of cis-[Co(en)$_2$(L)Cl]$^{2+}$ (en = 1,2-diaminoethane) with L = NH$_3$, NH$_2$CH$_3$, glyOC$_2$H$_5$, glyOCH$_3$, dl-alaOC$_2$H$_5$, NH$_2$CH$_2$CONH$_2$, and NH$_2$CH$_2$CN proceeds by dissociative interchange(I$_d$) mechanism from kinetic data, circular dichroism spectra, analyses of products, and the values of m(Grunwald-Winstein plot) using Y (solvent ionizing power) in aqueous solution and in mixed aqueous-organic solvent. It has been found that chloride replacement by water (aquation) for the series with L = NH$_3$ and NH$_2$CH$_3$ and chelation of ligand L to Co(Ⅲ) for the series with L = glyOC$_2$H$_5$, glyOCH$_3$, dl-alaOC$_2$H$_5$, NH$_2$CH$_2$CONH$_2$, and NH$_2$CH$_2$CN occurs, respectively. The rate constants on Hg$^{2+}$-induced reaction of the series except cis-[Co(en)$_2$(NH$_2$CH$_2$CN)Cl]$^{2+}$ were increased with increasing the contents of ethanol in mixed water-ethanol solvents. In mixed water-30${\%}$ organic solvents, the rate constants of the series except cis-[Co(en)$_2$(NH$_2$CH$_2$CN)Cl]$^{2+}$ have also been measured in the order 30${\%}$ 2-propanol-water > 30${\%}$ ethanol-water > water. However, the rate constants of cis-[Co(en)$_2$(NH$_2$CH$_2$CN)Cl]$^{2+}$ were reversed. The rate constants of the series with L= NH$_3$ and NH$_2$CH$_3$ were related to ligand field parameter (${\Delta}$), but those of the series with L = glyOC$_2$H$_5$, glyOCH$_3$, dl-alaOC$_2$H$_5$, NH$_2$CH$_2$CONH$_2$, NH$_2$CH$_2$CN were not. The reaction between the series and Hg2+ in aqueous media containing NO$_3^-$ has been investigated. The results for the reaction do not alter the mechanism, but the rate only was altered.

• Journal of Acupuncture Research
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• v.15 no.2
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• pp.331-348
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• 1998

Purpose: In order to study the therapeutic effect of TBR(Smilacis glabrae Rhizoma) aqua-acupuncture on the $HgCI_2$(mercury bichloride) intoxicated kidneys of rats. Methods: 10% TBR extract solution was aqua-punctured into the bilateral loci of Shin-su(BL23), every other day for 18 days, and the amount of leucocytes, erythrocytes, thrombocytes, hematocrit, hemoglobin, serum BUN and creatinine levels were measured and renal mercury level and histopathological findings were studied as well. The experimental groups were into nonmaI group, control group, sample I group, sample II group and sample III group. Results: 1. There were not any significant changes of leucocytes, erythrocytes and serum creatinine level in the TBR aqua-acupuncture group, saline aqua-acupuncture group and TBR aqua-acupuncture group respectively as compared with control group. 2. It showed a significant increase of hematocrit and hemoglobin level in the TBR aqua-acupuncture group on the 8th experimental day as compared with the control group. 3. It showed a significant increase of platelet in the TBR aqua-acupuncture group on the 8th experimental day, whereas significant decreases on the 18th experimental day as compared with the control group. 4. It showed a significant decrease of serum BUN level in the TBR aqua-acupuncture group on the 8th and 18th experimental day as compared with the control group. 5. It showed a significant decrease of renal mercury level in the TBR aqua-acupuncture group on compared with the control group. 6. In the histopathological findings, TBR aqua-acupuncture group showed slight atrophy of the renal tubules, mitosis and regeneration of tubular lining cells with visible nuclei, whereas the control group showed acute tubular necrosis, especially severe necrosis of tubular epithelial cells. Conclusions: Smilacis glabrae Rhizoma aqua-acupuncture on injured kidneys Induced by HgCl2 Intoxication in Rats increases hematocrit, hemoglobin and platelet level, and decreases BUN, renal mercury level. This suggests that Smilacis glabrae Rhizoma aqua-acupuncture helps to recover kidney from HgCl2 intoxication.

• The Korean Journal of Ecology
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• v.22 no.2
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• pp.89-94
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• 1999

Thirty-day-old seedlings of tomato (Lycopersicon esculentum) were treated with different concentrations of HgCl$_2$(0. 10 and 50 $\mu$M) for up to 20 days. and the detailed distribution of Hg absorbed and its toxicity in different plant parts (roots, stems and leaves) were investigated. The accumulation of Hg in plants increased with external Hg concentrations. and Hg is strongly retained by roots. Further. Hg content in leaves was various. showing more accumulation in older leaves. Seedlings exposed to toxic levels of Hg showed not only the reduction of dry weight and length of both shoot and root. and chlorophyll levels in leaves but also the enhancement of malondialdehyde (a lipid peroxidation product) formation in all plant parts investigated. These results suggest that physiological impairment of a plant exposed to Hg may be achieved by internal distribution of Hg absorbed and Hg-induced oxidative stress in different plant parts.

• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.140-145
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• 2009

All organisms are being exposed to harmful factors present in the environmental. The combined action of various factors is a distinguishing feature of modern life. An interaction between two chemicals is considered as synergistic when the effect produced is greater than the sum of the two single responses. The biological effects due to the combined action of ionizing radiation with the other factor are hard to estimate and predict in advance. In the current study, we investigated the synergistic effects between ionizing and $HgCl_2$ using fish hepatoma cells (PLHC-1 cells). The results showed a dramatic decrease of cell viability after simultaneous treatment of PLHC-1 cells with ionizing radiation and $HgCl_2$. Neiither of the two had any cytotoxic effect when treated alone. The cytotoxicity of ionizing radiation was enhanced in the presence of $HgCl_2$. The synergistic effects were observed after exposure of the PLHC-1 cells to ionizing radiation combined with $HgCl_2$. The synergistic interaction was due to an increase of irreversibly damaged cells after the combined exposure. Analysis of the extent of synergistic interaction enables to make quantitative estimation of irreversibly damaged cells after the combined exposure. The present study suggests that PLHC-1 cells can serve as rapid screening tools for detecting the toxicity of harmful factors.

• Journal of Preventive Medicine and Public Health
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• v.27 no.1 s.45
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• pp.11-24
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• 1994

The studies reported here were undertaken to investigate the effects of mercury chloride on immune system of Balb/c mouse employing a flexible tier of in vitro and in vivo assays. Mercury chloride inhibited the proliferative responses of spleen cells to lipopolysaccharide, pokeweed mitogen, and phytohemagglutinin as a dose-dependent manner. This inhibitory effect was observed not only when $HgCl_2$ was added 2nd or 3rd day of 3 days culture period but also when spleen cells was pretreated with $HgCl_2$ for 2 hours. Mercury chloride, however, potentiated the production of IgM and IgG from spleen cells. During the $HgCl_2$ administration by drinking for 3 weeks, the weight gain of mice was significantly blunted than that o control group mice, while no overt signs related to mercury toxicity were noted in any mice of experimental group. There was no change in thymus and spleen weights, and in histological findings of kidney, bone marrow of femur, thymus, spleen, and popliteal lymph node after 3 weeks of mercury exposure. However, $HgCl_2$ induced a significant increase of total serum IgM, IgG including $IgG_1,\;IgG_{2a}\;and\;IgG_{2b}$, and IgE in Balb/c mice. Treatment in vivo with anti-IL-4 monoclonal antibody significantly abrogated the $HgCl_2$-induced increase in total serum IgG1 and IgE. Whereas $HgCl_2$ potentiated total serum IgM and IgG, there was no difference in total serum hemagglutinin to SRBC (Sheep Red Blood Cell) between experimental and control group mice when these mice were immunized with SRBC. All these findings observed in Balb/c mice suggest that mercury perturbates well-orchestrated regulation of immune responses before developing histopathological changes in lymphoid tissues.