• Title/Summary/Keyword: $He^+$ ions

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Characteristics and Cancerostatic Activity of the Starfish Lectin (별불가사리 렉틴의 특성 및 암 세포 성장저해 효과)

  • Jeune, Kyung-Hee;Park, Chae-Soo;Park, Won-Hark;Choi, Soo-Jeong;So, Myung-Suk;Chung, See-Ryun
    • YAKHAK HOEJI
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    • v.41 no.4
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    • pp.421-432
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    • 1997
  • A new lectin was partially purified from starfish,Asterina pectinifera by means of physiological saline extraction, salt fractionation, ion exchange chromatography and hy droxyapatite chromatography, and it was named APL. The biochemical properties of the APL were characterized. In addition, its effects on lymphocyte mitogenicity and cancer cell agglutinability were tested. The APL agglutinated nonspecifically human erythrocytes and rabbit blood cells. Agglutinability was decreased to 30% of control activity below pH 5 and above pH 9 and was relatively unstable at increasing temperatures above 60$^{\circ}C$. The activity was reduced by addition of two kinds of metal ions, $Ba^{2+},\;Mn^{2+}$ and chelating agent, EDTA. APL was proved to be glycoproteins containing 9% sugars. For carbohydrate specificity, it was found that the activity of APL was inhibited by D(+)-glucosamine, D(+)-galactosamine, stachyose, N-acetyl-galactosamine and methyl-${\alpha}$-D-galactopyranoside among 35 sugars tested. In amino acid composition, the contents of acidic amino acids such as aspartic acid and glutamic acid were relatively high. This result suggest that the isoelectric point would be in a lower range. APL was found that it promotes the division of human lymphocytes. APL was proved to be a potent agglutinin for cancer cells such as HeLa, L929 and L1210 cells. Significant changes on the HeLa cell surfaces affected by APL were observed under the electron microscope.

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Biosorption of uranium by Bacillus sp.FB12 isolated from the vicinity of a power plant

  • Xu, Xiaoping;He, Shengbin;Wang, Zhenshou;Zhou, Yang;Lan, Jing
    • Advances in environmental research
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    • v.2 no.3
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    • pp.245-260
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    • 2013
  • Biosorption represents a technological innovation as well as a cost effective excellent remediation technology for cleaning up radionuclides from aqueous environment. In the present study, a bacteria strain FB12 with high adsorption rate of uranium ion was isolated from the vicinity of the nuclear power plant. It was tentatively identified as Bacillus sp.FB12 according to the 16S rDNA sequencing. Efforts were made to further improve the adsorption rate and genetic stability by UV irradiation and UV-LiCl cooperative mutagenesis. The improved strain named Bacillus sp.UV32 obtains excellent genetic stability and a high adsorption rate of 95.9%. The adsorption of uranium U (VI) by Bacillus sp.UV32 from aqueous solution was examined as a function of metal ion concentration, cell concentration, adsorption time, pH, temperature, and the presence of some foreign ions. The adsorption process of U (VI) was found to follow the pseudo-second-order kinetic equation. The adsorption isotherm study indicated that it preferably followed the Langmuir adsorption isotherm. The thermodynamic parameters values calculated clearly indicated that the adsorption process was feasible, spontaneous and endothermic in nature. These properties show that Bacillus sp.UV32 has potential application in the removal of uranium (VI) from the radioactive wastewater.

Measurement of secondary electron emission coefficient(${\gamma}$) with oblique low energy ion and work function ${\phi}_{\omega}$ of theMgO thin film in AC-PDPs

  • Park, W.B.;Lim, J.Y.;Oh, J.S.;Jeong, H.S.;Jung, K.B.;Jeon, W.;Cho, G.S.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.507-510
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    • 2004
  • Oblique ion-induced secondary electron emission coefficient(${\gamma}$) with low energy ..and work function ${\phi}_{\omega}$(${\theta}$ = 0 and ${\theta}$ = 20) of the MgO thin film in AC-PDPs has been measured by ${\gamma}$-FIB system. The MgO thin film has been deposited from sintered material under electron beam evaporation method. The energy of $He^+$ ions used has been ranged from 50eV to 150eV. Oblique ion beam has been chosen to be 10 degree, 20 degree and 30 degree. It is found that the higher secondary electron emission coefficient(${\gamma}$) has been achieved by the higher oblique ion beam up to inclination angle of 30 degree than the perpendicular incident ion beam.

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$Na^+$ Requirement of NADH dehydrogenase from an Extreme halophile, Halobacterium sp. EH10 Isolated from a Saltern in Korea (Halobacterium sp. EH10 NADH dehydrogenase의 $Na^+$ 요구성)

  • Bae, Moo;Lee, Jeong-Im
    • Microbiology and Biotechnology Letters
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    • v.19 no.2
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    • pp.153-157
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    • 1991
  • Intracellular enzymes of an extreme halophilic bacterium, Halobacterium sp. HE10, isolated from a saltern in Korea was investigated. The membrane-bound enzyme, NADH dehydrogenase, involved in electron transport system was stimulated by the addition of 2.0 M NaCl. The respiratory enzyme activities such as NADH oxidase and NADH dehydrogenase was decreased on removal of $Na^+$ ion and restored when replaced with cations like $K^+$, $Li^+$and $NH_{4}^{+}$ ions. Furthermore, their activities were affected by the anions such like carbonate, acetate, sulfate, chloride and nitrate at the presence of $Na^+$ion. Lactate dehydrogenase activity was highest at the asturated solution of NaCl and isocitrate dehydrogenase activity was a maximum level at 1.0 M NaCl. These results suggested that the enzyme activites of the respiratory chain in Halobacterium sp. EH10 was stimulated by the presence of $Na^+$ ion.

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High Dispersion Spectra of the Elliptical Planetary Ring Nebula NGC 6803

  • Lee, Seong-Jae;Hyung, Siek
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.80.1-80.1
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    • 2012
  • NGC 6803 is an elliptical ring shape planetary nebula. We analyzed the high dispersion spectra which had been observed with the Hamilton Echelle Spectrograph attached to the 3-m Shane telescope of Lick Observatory. We also investigated the low dispersion UV spectral data obtained with the 60-cm interstellar ultraviolet explorer. Diverse excitation lines were found from neutral to quadruply ionized ions. The temperature diagnostic lines indicate relatively low electron temperatures, i.e., $T_{\epsilon}{\leq}9500$ K for most lines except for [ClIV] - 11,500 K. In spite of its simplistic bi-laterally symmetrical elliptical shape, the nebula appears to be very complex of a hugh density range from 1300 to 80,000 $cm^3$. A comparison of the two epoch data suggests that the density increase occurred in the high excitation line zone near the inner boundary. We derived the chemical abundances of He, C, N, O, Ne, S, Ar, Cl, and K. The chemical abundances of NGC 6803 are enhanced compared with the average Galactic planetary nebula. Our self-consistent photo-ionization model study implies that the effective temperature of the central star is 90,000 K and its luminosity is 2400 $L_{\odot}$. The evolutionary track suggests that the progenitor of NGC 6803 was about 0.9 -- 1.0 $M_{\odot}$ star, which might be born from a metal-rich zone near the galactic disk, but now relocated into the present high Galactic latitude.

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A Development of the Low Energy Large Aperture Electron Beam Generator (저에너지 대면적 전자빔 발생장치 개발(II))

  • Woo, Sung-Hun;Lee, Kwang-Sik;Lee, Dong-In;Cho, Chu-Hyun;Choi, Young-Wook;Lee, Hong-Sik;Abroyan, M.
    • Proceedings of the KIEE Conference
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    • 1998.07e
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    • pp.1767-1769
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    • 1998
  • We have established a pulsed electron beam generation system with an energy of 200[keV], pulse repetition rate of 200[Hz], and several tens of [${\mu}s$] pulse width. The system is characterized by a cold cathode that is simpler than the hot cathode. Target object does not need to be scanned because of large aperture electron beam of 300[$cm^2$]. Electron source is secondary electrons that are generated when the ions from the glow discharge collide on the cathode surface. In this paper, the discharge current characteristics are investigated experimentally as a function of He gas pressure in order to obtain stable glow discharge. And computer simulations are carried out as a preliminary study for the development of low energy large aperture electron beam generator. The variation of electon beam current is investigated as a function of rising time of high voltage when 20[kV] potential is applied in 20[mTorr] pressure.

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Discharge and Luminous Characteristics of Coplanar Type Xe Plasma Flat Lamp (면방전형 Xe 플라즈마 평판 램프의 방전 및 발광 특성)

  • Kim, Hyuk-Hwan;Lee, Won-Jong
    • Korean Journal of Materials Research
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    • v.21 no.10
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    • pp.532-541
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    • 2011
  • The Xe plasma flat lamp, considered to be a new eco-friendly LCD backlight, requires a further improvement of its luminance and luminous efficiency. To improve the performance of this type of lamp, it is necessary to understand the effects of the discharge variables on the luminous characteristics of the lamp. In this study, the luminous characteristics of a coplanartype Xe plasma flat lamp with a teeth-type electrode pattern were analyzed while varying the gas composition, gas pressure and input voltage. The effects of the phosphor layer on the discharge and the luminous characteristics of the lamp were also studied. The luminous efficiency of the coplanar-type Xe plasma flat lamp improved as the Xe input ratio and gas pressure increased. Higher luminous efficiency was also obtained when helium (He) was used as a buffer gas and when a phosphor layer was fabricated on the electrode region. In contrast, the luminous efficiency was reduced with increasing the input voltage. It was found that the infrared emissions from the lamp were affected by the Xe excitation rate in the plasma, the Xe gas density, the collisional quenching of excited Xe species by gas molecules, and the recombination rate between the Xe ions and electrons.

Optical properties of Ag/$Ge_1Se_1Te_2$ material with secondary Ag layer adoption (두 번째 Ag 층을 적용한 Ag/$Ge_1Se_1Te_2$ 물질의 광학적 특성 연구)

  • Kim, Hyun-Koo;Han, Song-Lee;Kim, Jae-Hoon;Koo, Sang-Mo;Chung, Hong-Bay
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.191-192
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    • 2008
  • For phase transition method, good record sensitivity, low heat radiation, fast crystallization and hi-resolution are essential. Also, a retention time is very important part for phase-transition. In our past papers, we chose composition of $Ge_1Se_1Te_2$ material to use a Se factor which has good optical sensitivity than conventional Sb. Ge-Se-Te and Ag/$Ge_1Se_1Te_2$ samples are fabricated and irradiated with He-Ne laser and DPSS laser to investigate a reversible phase change by light. Because of Ag ions, the Ag layer inserted sample showed better performance than conventional one. We should note that this novel one showed another possibility for phase-change random access memory.

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Crystal Structures and Luminescence Properties of [Ln(NTA)2·H2O]3- Complexes (Ln = Sm3+, Eu+3, Gd3+, Tb3+, Ho3+, and NTA = Nitrilotriacetate)

  • Kang, Jun-Gill;Kang, Hee-Jung;Jung, Jae-Sun;Yun, Sock-Sung;Kim, Chong-Hyeak
    • Bulletin of the Korean Chemical Society
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    • v.25 no.6
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    • pp.852-858
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    • 2004
  • Crystal structures of lanthanide complexes with NTA (NTA = nitrilotriacetate) are reported. The complexes of $[Ln(NTA)_2{\cdot}H_2O]^{3-}$ (Ln = Sm, Eu, Gd, Tb and Ho) crystallize in the orthorhombic space group Pccn. In the structures, the trivalent lanthanide ions are completely encapsulated via coordination to the two nitrogen atoms and the six carboxylate oxygen atoms of the two NTA ligands, and one water oxygen atoms. The complexes form a slightly distorted capped-square-antiprism polyhedron. Of the complexes, $[Eu(NTA)_2{\codt}H_2O]^{3-}$,\;[Tb(NTA)_2{\cdot}H_2O]^{3-}\;and\;[Dy(NTA)_2{\cdot}H_2O]^{3-}$ excited at the 325 He-Cd line produce very characteristic luminescence features, arising mostly from the f ${\to}$ f transitions. The absolute quantum yields of these complexes are determined at room temperature. Surprisingly, the $[Dy(NTA)_2{\cdot}H_2O]^{3-}$ complex is more luminescent than the $[Eu(NTA)_2{\cdot}H_2O]^{3-}\;and\;[Tb(NTA)_2{\cdot}H_2O]^{3-}$ complexes.

Heavy Metals Immobilization in Soil with Plant-growth-promoting Rhizobacteria and Microbial Carbonate Precipitation in Support of Radish Growth

  • He, Jing;Zhang, Qiuzhuo;Achal, Varenyam
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.223-229
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    • 2020
  • The application of plant-growth-promoting rhizobacteria (PGPR) supports the growth of plants in contaminated soil while ureolytic bacteria can immobilise heavy metals by carbonate precipitation. Thus, dual treatment with such bacteria may be beneficial for plant growth and bioremediation in contaminated soil. This study aimed to determine whether the PGPR Pseudomonas fluorescens could work in synergy with ureolytic bacteria to assist with the remediation of cadmium (Cd)- and lead (Pb)-contaminated soils. Pot experiments were conducted to grow radish plants in Cd- and Pb-contaminated soils treated with PGPR P. fluorescens and the results were compared with dual inoculation of P. fluorescens combined with ureolytic Staphylococcus epidermidis HJ2. The removal rate of the metals from the soil was more than 83% for Cd and Pb by the combined treatment compared to 17% by PGPR alone. Further, the dual treatment reduced the metal accumulation in the roots by more than 80%. The translocation factors for Cd and Pb in plant tissues in both treatments remained the same, suggesting that PGPR combined with the carbonate precipitation process does not hamper the transfer of essential metal ions into plant tissues from the soil.