• Title/Summary/Keyword: $H_2$ uptake

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A Study on Cochineal Dyeing by Various Mordants and pH Conditions II -Treatment on Wool Fabric- (염욕의 pH와 매염제의 변화에 따른 코치닐의 염색성 연구 II -양모직물을 중심으로-)

  • Kim Kyung-Sun;Kim Jong-Jun;Jeon Dong-Won
    • The Research Journal of the Costume Culture
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    • v.13 no.2 s.55
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    • pp.248-254
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    • 2005
  • In this study, wool fabric specimens were pre-mordanted using Sn, Al, Cu, Cr, and Fe, and subsequently dyed using cochineal, maintaining the pH of the dye bath constant using pH buffer solutions of 4, 5, 6, 7, and 8. In the case of wool fabric specimen, regardless of the type of mordanting agents, peak dye-uptake amount was obtained at the acidic region, pH 4, and above pH 6, the dye-uptake amount decreased remarkably. Differing from the cotton fabric case, the dyed wool fabric specimen exhibited red shade even in the case of non-mordanting, at the region of pH values of 4 and 5. It is presumed that in the acidic dye bath the effect of cationic amine group present in the structure of wool fiber molecules took place. The amount of color difference, among the mordanting agents, due to the increase of pH value, was highest for the Fe mordanting case. It seems, therefore, that the Fe mordanting is affected most by the pH value.

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The Recovery of Heavy Metals Using Encapsulated Microbial Cells

  • Park, Joong-Kon;Jin, Yong-Biao;Park, Hyung-Woo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.2 no.2
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    • pp.132-135
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    • 1997
  • We prepared capsules containing Saccharomyces cerevisiae and Zoogloea ramigera cells for the removal of lead(II) and cadmium ions. Microbial cells were encapsulated and cultured in the growth medium. The S.cerevisiae cells grown in the capule did not leak through the capsule membrane. The dried cell density reached to 250 g/l on the basis of the inner volume of the 2.0 mm diameter capsule after 36 hour cultivation. The dry whole cell expolymer density of encapsulated Z.ramigera reached to 200 g/L. The capsule was crosslinked with triethylene tetramine and glutaric dialdehyde solutions. The cadmium uptake of encapsulated whole cell expolymer of Z.ramigera was 55mg Cd/g biosorbent. The adsorption line followed well Langmuir isotherm. The lead uptake of the encapsulated S. cerevisiae was about 30 mg Pb/g biomass. The optimum pH of the lead uptake using encapsulated S. cerevisiae was found to be 6. Freundlich model showed a little better fit to the adsorption data than Langmuir model 95 percent of the lead adsorbed on the encapsulated biosorbents was desorbed by the 1 M HCl solution. The capsule was reused 50 batches without loosing the metal uptake capacity. And the mechanical strength of the crosslinked capsule was retained after 50 trials.

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Study on Adsorption Features of Arsenic onto Lepidocrocite (레피도크로사이트(lepidocrocite) 표면의 비소 흡착 특성 규명)

  • Lee, Woo-Chun;Jeong, Hyeon-Su;Kim, Ju-Yong;Kim, Soon-Oh
    • Economic and Environmental Geology
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    • v.42 no.2
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    • pp.95-105
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    • 2009
  • Systematic studies are performed for arsenic adsorption on synthesized lepidocrocite. The synthesized lepidocrocite with high surface area of $94.8\;g/m^2$ has shown that the point of zero charge(PZC) is 6.57 determined by potentiometric titration, suggestive of high capacity of arsenic removal. Results show that arsenite[As(III)] uptake by synthesized lepidocrocite is greater than that of arsenate[As(V)] at pH $2{\sim}12$, indicating that the lepidocrocite has high affinity toward arsenite rather than arsenate. Adsorption of arsenate decreases with increasing pH from 2 to 12, whereas arsenite sorption increases until pH 8.0, and then decreases dramatically with increasing pH, suggesting that changes in surface charge of the lepidocrocite as a function of pH playa important role in aresinc uptake by the lepidocrocite. Upon kinetic experiments, our results demonstrate that both arsenite and arsenate sorption on the lepidocrocite increases rapidly for the first 4 h followed by little changes during the duration of the experiment, showing that adsorption plays a key role in aresenic uptake by the lepidocrocite. Our results also show that power function and elovich models are the best fit for the adsorption kinetics of arsenite and aesenate on the lepidocrocite.

Response of N. Sources to Nutrient Uptake of Tobacco Plant (질소원(窒素源)에 따른 담배식물의 양분흡수반응(養分吸收反應))

  • Lee, Yun-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.4
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    • pp.413-418
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    • 1985
  • Tobacco plant(8-leaf seedlings) were grown on water culture fertilized with different N sources ($NO_3-N$, $NH_4-N$, $NO_3+NH_4-N$) during 15 days. Daily uptake of nutrients and inorganic constituents in plants were investigated in relation to growth responses of them. 1. Nitrate-fed plant showed higher daily uptake of inorganic cations than those in other treatments, and reached about two times higher uptake of nitrogen and three times more uptake of cations (K, Ca, Mg). Potassium was preferentially uptaken at a very fast rate from the beginning after treatment. Also $NO_3-N$ tended to be taken up selectively by the plant from the mixture of nitrate and $NH_4-N$. 2. The initial pH (pH 6.0) of culture medium drastically changed into acid (pH 4.0) in the $NH_4-N$ medium, but into slightly higher (pH 6.4) in the nitrate when measured after exposure of 24 hours. The mixture also tended to show an acidity but much weaker than $NH_4-N$ solution. 3. Nitrate-fed plant had a normal growth pattern but $NH_4-N$ fed plant almost stopped growing. Those plants containing both nitrate and ammonium N were also showed very poor growth.

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Brain Uptake and the Analgesic Effect of Oxytocin - its Usefulness as an Analgesic Agent

  • Kang, Young-Sook;Park, Ji-Hyun
    • Archives of Pharmacal Research
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    • v.23 no.4
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    • pp.391-395
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    • 2000
  • To establish the usefulness of oxytocin (OT) as an analgesic for women in delivery, the pharmacokinetic parameters and blood-brain barrier (BBB) permeability of [$^3H$] OT were obtained using an intravenous injection technique or the internal carotid artery perfusion/capillary depletion (ICAP/CDM) method. Brain uptake of OT was similar to that of sucrose, plasma space marker, indicating that OT has a poor BBB permeability. Moreover, the analgesic effects of OT injected through the jugular vein on nociception were evaluated by the tail-flick method. The antinociceptive effects of OT injected at a dose of 0.2 ${m}g/kg$or 2 ${m}g/kg$ were dose-dependent. In addition, the analgesic effects of OT on the CNS were unaffected by naloxone, a m-receptor antagonist. In a similar manner to the opioid system, OT may play a modulatory role in antinociception.

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Covalent Organic Frameworks for Extremely High Reversible $CO_2$ and $H_2$ Uptake Capacity : A Multiscale Simulation Approach (우수한 가역적 이산화탄소 및 수소 저장성능을 가지는 공유결합성 유기적 골격구조체에 관한 다중스케일 접근법을 이용한 연구)

  • Choi, Yoon Jeong;Choi, Jung Hoon;Choi, Kyung Min;Kang, Jeung Ku
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.113.2-113.2
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    • 2010
  • We report that the novel covalent organic frameworks (COFs) are capable of reversibly providing an extremely high uptake capacity of carbon dioxide and hydrogen at room temperature. These COFs are designed based on the multiscale simulations approach via the combination of ab initio calculations and force-field calculations. For this goal, we explore the adsorption sites of carbon dioxide and hydrogen on COFs, their porosity, as well as carbon dioxide adsorption isotherms. We identify the binding sites and energies of $CO_2$ on COFs using ab initio calculations and obtain the carbon dioxide adsorption isotherms using grand canonical ensemble Monte Carlo calculations. Moreover, the calculated adsorption isotherms are compared with the experimental values in order to build the reference model in describing the interactions between the $CO_2/H_2$ and the COFs and in predicting the $CO_2$ and $H_2$ adsorption isotherms of COFs. Finally, we design three new COFs, 2D COF-05, 3D COF-05 (ctn), and 3D COF-05 (bor), for the high capacity $CO_2/H_2$ and $H_2$ storage.

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Cloning of the Gene for Na$^{+}$/Serine-Threonine Symporter (sstT) from Haemophilus influenzae Rd and Characteristics of the Transporter

  • Kim, Young-Mog
    • Journal of Microbiology
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    • v.41 no.3
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    • pp.202-206
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    • 2003
  • A protein, exhibiting a high similarity to the major serine transporter of Escherichia coli, SstT, was found in Haemophilus influenzae Rd. A Na$\^$+/-stimulated serine transport activity was also detected in the cells. The gene (sstT) for the Na$\^$+//serine symporter from the chromosome of H. influenzae was cloned, and the properties of the transporter investigated. The serine transport activity was stimulated by Na$\^$+/. The uptake of Na$\^$+/ was elicited by the addition of serine or threonine into the cells, supporting the idea that these amino acids are transported by a mechanism of Na$\^$+//substrate symport. No uptake of H$\^$+/ was elicited by the influx of serine. The serine transport via the SstT of H. influenzae was inhibited by excess threonine, which was used as another substrate. The $K_{m}$ and the $V_{max}$ values for the serine transport were 2.5 ${\mu}$M and 14 nmol/min/mg protein, respectively.

Sugar Transoprt in Conidia of Neurospora crassa (붉은 빵곰팡이 세포의 당운반)

  • 이호자
    • Korean Journal of Microbiology
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    • v.13 no.2
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    • pp.45-50
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    • 1975
  • Uptake of $^{14}C$-sorbose and $^{14}C$-3-O-methylglucose by ungerminated conidia of Neurospora crassa was measured by means of the millipore filter technique. Initial rates of jptake of both sorbose and 3-O-methylglucose show a marked dependence optimal pH for uptake of both sugars is close to 4.75. When ungerminated conidia are "starved" with buffer for a prolonged period of time prior to assaying their transport capacity and mycelia, no de-repression of the glucose-repressible sugar transport system is effectuated in contrast to the findings for germinated conidia.d conidia.

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AUTORADIOGRAPHIC STUDY OF THE COLLAGENASE - INFLUENCE ON THE RAT PERIODONTIUM AFTER EXPERIMENTAL TOOTH MOVEMENT (실험적(實驗的) 치아이동후(齒牙移動後) 교원효소(膠原酵素) 투여(投與)가 치근막(齒根膜) 섬유(纖維)의 변화(變化)에 미치는 영향(影響)에 관(關)한 자기방사법적(自己放射法的) 연구(硏究))

  • Hong, Sung Joon;Suhr, Cheong Hoon
    • The korean journal of orthodontics
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    • v.20 no.2
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    • pp.227-245
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    • 1990
  • The purpose of this study was to analyze the reorganization of periodontal ligament after collagenase treatment with autoradiography. The author compared the collagenase-treated experimental group and no-treated experimental group with control group. Fourty eight Sprague-Dawley rats were divided into nine groups, including normal control and immediate group. Closed coil springs were used between the upper incisors and the first molars with 100 grams. Collagenase and $^3H-proline$ were adminstered and the samples were sacrificed and sectioned. After being dipped into the NTB-3 emulsion the samples were analyzed with light microscope under H/E stain. Data were analyzed by t-test and ANOVA. The results were as follows: 1) Generally collagenase-treated groups got more $^3H-proline$ uptake than no-treated groups. 2) Compared with normal control group, collagenase-treated group had the same $^3H-proline$ uptake in amount at 21th day. 3) Among cemento-enamel junction, middle, apex areas, cementa-enamel junction area of collagenase-treated group arrived at normal control level earlier than no-treated group. 4) Cemento-enamel junction area had the most $^3H-proline$ incorporation amount in no-treated group, but apex area had the most in collagenase group.

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Effects of D-Fructose on the Uptake of Iron by the Intestinal Brush-Border Membrane Vesicles from Rats.

  • Kim, Ok-Seon;Lee, Yong-Bok;Oh, In-Joon;Koh, Ik-Bae;Lee, Yeong-Woo
    • Journal of Pharmaceutical Investigation
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    • v.24 no.3
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    • pp.11-18
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    • 1994
  • We have studied the iron uptake by the purified brush-border membrane vesicles (BBMVs) to determine the effect of fructose on the absorption of iron. BBMVs were prepared by the modified calcium precipitation method, The degree of purification was routinely assessed by the marker enzyme, alkaline phosphatase, and the functional integrity was tested by $D-[1-^3H]glucose$ uptake. The appearance of membrane vesicles was shown by transmission electron microscopy (TEM). The uptakes of complexes of labeled iron $[^{59}Fe]$ with fructose and ascorbate were measured with a rapid filtration technique, The uptake rate and pattern of the two iron-complexes, Fe(III)-fructose and Fe(III)-ascorbate, were also observed. A typical overshooting uptake of D-glucose was observed with peak value of $2{\sim}3$ times higher concentration than that at equilibrium. This result was similar to other studies with BBMVs. TEM showed that the size of BBMVs was uniform and we can hardly find any contaminants, Fe(III)-fructose has the higher value of $V_{max}$ and the lower value of Km than those of Fe(III)-ascorbate, respectively. It may be concluded that D-fructose is more effective in promoting the iron absorption than ascorbate.

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