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Cloning of the Gene for Na$^{+}$/Serine-Threonine Symporter (sstT) from Haemophilus influenzae Rd and Characteristics of the Transporter  

Kim, Young-Mog (Institute of Agricultural Science & Technology, Kyungpook National University)
Publication Information
Journal of Microbiology / v.41, no.3, 2003 , pp. 202-206 More about this Journal
Abstract
A protein, exhibiting a high similarity to the major serine transporter of Escherichia coli, SstT, was found in Haemophilus influenzae Rd. A Na$\^$+/-stimulated serine transport activity was also detected in the cells. The gene (sstT) for the Na$\^$+//serine symporter from the chromosome of H. influenzae was cloned, and the properties of the transporter investigated. The serine transport activity was stimulated by Na$\^$+/. The uptake of Na$\^$+/ was elicited by the addition of serine or threonine into the cells, supporting the idea that these amino acids are transported by a mechanism of Na$\^$+//substrate symport. No uptake of H$\^$+/ was elicited by the influx of serine. The serine transport via the SstT of H. influenzae was inhibited by excess threonine, which was used as another substrate. The $K_{m}$ and the $V_{max}$ values for the serine transport were 2.5 ${\mu}$M and 14 nmol/min/mg protein, respectively.
Keywords
Haemophilus influenzae Rd; Na$^{+}$/serine-threonine symporter;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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