Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Cloning of the Gene for Na$^{＋}$/Serine-Threonine Symporter (sstT) from Haemophilus influenzae Rd and Characteristics of the Transporter

  • Kim, Young-Mog (Institute of Agricultural Science ＆ Technology, Kyungpook National University)
  • Published : 2003.09.01
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Abstract

A protein, exhibiting a high similarity to the major serine transporter of Escherichia coli, SstT, was found in Haemophilus influenzae Rd. A Na$\^$＋/-stimulated serine transport activity was also detected in the cells. The gene (sstT) for the Na$\^$＋//serine symporter from the chromosome of H. influenzae was cloned, and the properties of the transporter investigated. The serine transport activity was stimulated by Na$\^$＋/. The uptake of Na$\^$＋/ was elicited by the addition of serine or threonine into the cells, supporting the idea that these amino acids are transported by a mechanism of Na$\^$＋//substrate symport. No uptake of H$\^$＋/ was elicited by the influx of serine. The serine transport via the SstT of H. influenzae was inhibited by excess threonine, which was used as another substrate. The $K_{m}$ and the $V_{max}$ values for the serine transport were 2.5 ${\mu}$M and 14 nmol/min/mg protein, respectively.

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