• Journal of Plant Biotechnology
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• 제44권2호
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• pp.178-190
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• 2017

$Fragaria{\times}ananassa$, a strawberry evolved from hybridization between F. virginiana and F. chiloensis, is a globally cultivated and consumed fruit crop valued for its flavor and nutritional value. Flavor and quality of fruits are determined by factors such as sugars and organic acids present during fruit development. These characteristics are highly subjective in different genotypes and affected by various environmental factors. In this study, we analyzed contents of major sugar compounds including fructose, glucose and sucrose by HPLC analysis in four cultivars namely, Maehyang, Seolhyang, Festival and Sweet Charlie. We identified 55 genes related to fructose, glucose, sucrose and soluble sugar regulation whose expression were analyzed in four cultivars at three developmental stages of the fruit namely, green, white and ripened stages. Expression of these genes across these progressive fruit developmental stages varied among cultivars. Among the 55 genes, genes FaFru3, FaSuc11 and FaGlu8 revealed differential patterns of expression along developmental stages of the fruit in high and low sugar-containing genotypes, respectively and may be putative candidates for sugar content in strawberries. Expression of genes are discussed with regard to corresponding sugar content in these genotypes. Further analysis and application of these genes may be valuable in developing high sugar containing cultivars via marker-assisted breeding.

• 한국식물병리학회지
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• 제14권6호
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• pp.735-737
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• 1998

A severely wilting of strawberry caused by a Phytophthora sp. has occurred houses after planting in vinyl-houses field at Ssanglim and Anlim areas Kyungbuk in Korea from October in 1997. Phytophthora sp. isolated from diseased tissues of the crown of strawberry. Browning rot of inner crown and root resulted in wilt and eventual death of the plant. The causal fungus was identified as Phytophthora cactorum. Sporangia were ovoid, conspicuously papillate, caducous and measured 30.0~56.6$\times$23.8~35.2 (av. 39.3~29.9) ${\mu}{\textrm}{m}$. Sexuality of the fungus was homothallic. Oogonia were sperical and 23.3~32.3 (av. 29.0) ${\mu}{\textrm}{m}$ in size. Most ahteridia were paragynous and measured av. 10.2~12.2 ${\mu}{\textrm}{m}$. Cardinal temperature for growth at minimum, optium, maximum were recorded at 7, 20~25, and 32$^{\circ}C$, respectively. The fungus show strong pathogenicity to strawberry. This is the first report of strawberry caused by Phytophthora cactorum in Korea.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.37-40
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• 2004

• 식물조직배양학회지
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• 제28권3호
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• pp.171-178
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• 2001

딸기의 기내대량번식 체계를 확립하기 위하여 딸기 잎절편체로부터 부정아 형성에 미치는 2,4-D, BAP, 한천, 자당 및 미오이노시톨의 영향에 관하여 실험하였다. 부정아 형성은 MS배지에 0.1 mg/L 2,4-D와 3 mg/L BAP, 0.4% 한천 첨가배지에서 가장 촉진적이었으며, 2,4-D 단독배지에서는 부정아 형성이 유도되지 아니하였다. 0.4% 한천을 첨가한 배지에서 부정아 형성은 1%한천 첨가배지에 비하여 약 3배가 촉진되었으나 투명화 현상이 심하였다. 따라서 딸기 잎 절편을 0.05mg/L 2,4-D, 1 mg/L BAP, 0.8% 한천,30 g/L 자당 및 100mg/L의 미오이노시톨에서 배양하였을 때 건전한 유식물체가 형성되었다.

• Journal of Plant Biotechnology
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• 제45권2호
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• pp.90-101
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• 2018

Strawberry (Fragaria ${\times}$ ananassa) is a globally-cultivated and popular fruit crop, prized for its flavor and nutritional value. Sweetness, a key determinant of fruit quality, depends on the sugar composition and concentration. We selected eight strawberry cultivars based on the fruit soluble solids content to represent high and low sugar content groups. The average soluble solid content was $13.6^{\circ}Brix$ (Okmae, Geumsil, Aram, and Maehyang) and $2.9^{\circ}Brix$ (Missionary, Camino Real, Portola, and Gilgyung53), for the high and low sugar content groups, respectively. Sucrose was the main sugar in the cultivars with high sugar content, whereas fructose was the main component in the low sugar content cultivars. Fruit starch concentration ranged from $3.247{\pm}0.056$ to $3.850{\pm}0.055g/100g$, with a 12% higher concentration in the high sugar content cultivars. Additionally, we identified 41 sugar metabolism-related genes in Fragaria ${\times}$ ananassa and analyzed the relationship between their transcripts and the sugar accumulation in fruit. FaGPT1, FaTMT1, FaHXK1, FaPHS1, FaINVA-3, and FacxINV2-1 were highly expressed in the high sugar content cultivars, while FapGlcT, FaTMT2-1, FaPHS2-1, FaSUSY1-1, and FaSUSY1-2 were highly expressed in the low sugar content cultivars. In general, a greater number of genes encoding sugar transporters or involved in sugar synthesis were highly expressed in the high sugar content cultivars. Contrarily, genes involved in sugar degradation were preferentially transcribed in the low sugar content cultivars. Although gene expression was not perfectly proportional to sugar content or concentration, our analysis of the genes involved in sugar metabolism and accumulation in strawberries provides a framework for further studies and for the subsequent engineering of sugar metabolism to enhance fruit quality.

• The Plant Pathology Journal
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• 제21권1호
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• pp.83-85
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• 2005

Ditylenchus acris was isolated from diseased strawberry plants. Frequently, D. acris and Aphelenchoides fragariae occur together in a strawberry plant. Both species appeared very similar in the shape, length, swimming behavior and causing symptoms, and difficult to distinguish them by a stereomicroscope. But they were easily distinguished under a compound microscope especially by their tail shape, median bulb, vulva position, and bursa.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.436-436
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• 2005

• 생물환경조절학회지
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• 제28권4호
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• pp.302-310
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• 2019

본 연구는 딸기(Fragaria ${\times}$ ananassa Duch.) 두 품종인 대왕과 설향을 LED 조명으로 일몰 후부터 6시간 동안 2018년 11월부터 이듬해 1월까지 야간 보광 처리한 후, 각각 50%와 100% 착색되었을 때, 과실을 수확하여 딸기 과실의 경도, 식물화합물 및 항산화 활력을 측정하여 LED 광과 성숙도에 따른 과실 품질변화를 확인하고자 실시하였다. 딸기 과일의 경도에서는, 두 품종 모두 적색 LED를 보광했을 때, 50%뿐만 아니라 100% 익은 과실에서 유의하게 높았다. 당도 또한 50% 착색된 과실을 비교한 경우, 적색 LED 광이 품종 모두에서 다른 광처리 보다 유의하게 높게 나타났다. 반면에, 딸기의 산도에서는 LED 광 처리구보다는 대조구에서 50%뿐만 아니라 100% 착색된 과실에서도 높게 나타났다. 페놀화합물의 경우, LED 광을 처리한 딸기보다는 대조구 상태에서 생육한 딸기 과실의 함량이 월등히 높았다. 하지만 플라보노이드와 안토시아닌 함량에서는 LED 광의 영향은 미미하였다. 식물화합물의 경우는 LED 광보다는 익어가면서 성숙도에 따라 그 함량의 변화가 달라지는 경향이 크다. 또한, DPPH 및 ABTS 항산화능의 경우는 성숙도 및 LED 보광에 의한 차이는 없었다. 따라서 본 연구 결과로 미루어 보아, 과실의 당도와 유통 측면에서는, LED 보광이 충분히 과실 품질에 도움 될 것으로 사료된다.

• 원예과학기술지
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• 제33권5호
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• pp.625-632
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• 2015

Drought is a severe abiotic stress that affects global crop production. A drought model was created for 'Toyonoka' Fragaria ${\times}$ ananassa, and the effects of drought stress on contents of proline, sugars, and antioxidant enzyme activities were investigated. Strawberry transplants with identical growth were chosen for the experiments and the randomized design included four replications (10 plants per block). The experimental sets differed in the moisture level of the culture medium relative to the range of moisture content as follows: control, 70-85%; mild drought stress, 50-60%; moderate drought stress, 40-50%; and severe drought stress, 30-40%. Drought stress was imposed by limiting irrigation. Plants were sampled and physiological parameters w ere measured on 0, 2, 4, 6, 8, and 10 days after the commencement of droughts tress. The water potential of strawberry leaves decreased in the plants under mild, moderate, and severe stress during the course of the water stress treatment and exhibited a significant difference from the control. Strawberry leaves subjected to drought stress had higher accumulation of proline, sugars, and malondialdehyde, and higher activities of superoxide dismutase, peroxidase, and catalase than leaves of control plants. Malondialdehyde levels increased in parallel with the severity and duration of drought stress. By contrast, antioxidant enzyme activity displayed dynamic responses to drought stress, first increasing and subsequently decreasing as the severity and duration of drought stress increased. These results suggest that strawberry plants respond to drought stress by altering the activities of antioxidant enzymes and the levels of osmotically active metabolites. These biochemical response changes may confer adaptation to drought stress and improve the capacity of plants to withstand water-deficit conditions.

• 식물조직배양학회지
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• 제25권5호
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• pp.347-351
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• 1998

딸기 '수홍'의 기내배양된 잎과 탁엽 절편체로부터 기관형성을 통한 식물체 재생 방법을 확립하고자 실험을 수행하였다. 엽절편체 배양에서는 생장조절제 처리에 따라 NAA 첨가시에는 뿌리 재생, BA 첨가시에는 신초 재생이 이루어졌으며, BA 1.0㎎/L + NAA 0.2 ㎎/L 처리구에서 재생률 31.1%, 절편체당 신초수 1.7개로 가장 양호하였다. 탁엽절편체 배양에서는 생장조절제와 광조건의 처리를 실시하였는데, 명조건에서는 생장조절제 처리에 따른 뚜렷한 경향을 발견할 수 없었으나, 암조건에서는 고농도 BA와 저농도 NAA의 혼용처리가 신초 재생에 필요함을 알 수 있었다. 탁엽절편체는 BA 2.0 ㎎/L + NAA 0.1㎎/L 처리구에서 재생률 44.4%, 절편체당 신초수 4.0개로 가장 양호한 반응을 나타내었다. 재생된 신초는 NAA 0.1㎎/L 가 첨가된 MS배지에서 발근되었으며, 이어서 인공토양(vermiculite : perlite = 1 : 1)에서 성공적으로 활착을 유도할 수 있었다.