• 대한수의학회지
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• 제26권1호
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• pp.103-108
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• 1986

The oil emulsion and alhydrogel pilli vaccines were prepared from a strain(O9: K35, K99, F41) of enterotoxigenic Escherichia coli isolated from calves with diarrhea and their immunogenicity was tested in guinea-pigs, pregnant goats and cows. Serum antibody responses to K99 and F41 antigens in guinea-pigs given experimental oil and gel vaccines peaked at 4 and 6 weeks after vaccinations. At that time, the mean hemagglutination inhibition titers to K99 and F41 antigens in guinea-pigs given oil vaccine were 1:25 and 1:1, 218 and those given gel vaccine were 1:54 and 1:724 respectively. Agglutinin titers in pregnant goats given the oil vaccine were significantly higher(mean 1:2,347) compared to those of control group(mean 1:160). Less than 12.5% of goatlings from vaccinated goats developed scours compared to nearly 100% in control group after oral challenge with enterotoxigenic Escherichia coil within 24 hours after birth. The highest agglutinin titers of cow serum and colostrum and of the serum of calves 48 hours after birth from cows given oil vaccine were 1:256, 1:512 and 1:64 respectively. On the other hand, those titers of serum and colostrum and of the serum of nursing calves from nonvaccinated cows were 1:8, 1:16 and 1:20 respectively. The protective efficacy of the oil emulsion vaccine was 72.1% under field conditions. These results strongly indicated that the vaccine could be applied for protection of diarrhea caused by enterotoxigenic Escherichia coli in calves.

• 한국작물학회지
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• 제36권3호
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• pp.226-235
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• 1991

트리티케일 품종육성의 기초자료를 제공하기위해, 6배체 트리티케일인 신기호밀(TC)과 6배체 보통밀 5개 품종을 교잡한 잡종 초기세대의 교잡친화성, 발아율, 및 화분의 활력등을 검토한 시험결과를 요약하면 다응과 같다 1. 신기호밀(TC)과 밀의 교배에서 교잡율은 조합에 따라 28.8～41.8%로, 평균 34.1%로 나타났다. 그러나 이들의 역교배에서는(밀X신기호밀) 교잡율이 극히 낮았다. F$_1$에 신기호밀을 환분친으로 여교잡(F$_1$/P$_1$)했을 때는 평균 3.99%, 밀을 환분친으로 여교잡(F$_1$,P$_2$) 했을 때는 평균 21.9%의 교잡율을 보였고, F$_1$을 자식시킨 에서는 평균 3.61%의 임실율을 보였다. 2. 트리티케일과 밀의 F$_1$에 양친을 여교접할 때 F$_1$/밀이 /F$_1$보다. 트리리케일/F$_1$이 F$_1$/트리티케일보다 교잡율이 높았다. 3. 모든 단교배 F$_1$ 종자의 발아율은 80% 이상이었으며 트리티케일/밀에서 F$_1$/P$_2$세대는 평균81%, F$_1$/P$_1$은 62%, F$_2$는 66.3%로 세대별로 차이가 있었다. 4. 트리티케일 품종 신기호밀을 화분친으로 사용하여 밀과 교배할 경우 배란형성이 안되었으며, 이때의 배란게놈 조성은 AAABBBDDR 이었다. 5. 트리티케일과 밀을 교잡한 F$_1$의 화분 임성은 평균 30.2%로 나타났다. 6. 트리티케일과 밀의 F$_1$의 화분 임성과 교잡율(F$_1$, F$_2$, F$_1$/P$_2$)간에는 정의 상관관계가 있었다

• 한국연초학회지
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• 제20권1호
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• pp.33-39
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• 1998

Survival, growth and reproduction of the cigarette beetle, Lasiodema sewicome F., were studied on flue-cured (NC82), air-cured (Br.21), aromatic (Izmir) tobacco and artificial diet (wheat feed 95% + yeast 5%). Cigarette beetle adults occurred from first generation in the flue-cured and aromatic tobacco, but no adults emerged in air-cured tobacco after the 2nd generations. The head capsule widths of larvae of the cigarette beetles L sewicome F.1 reared on different diets at 2$0^{\circ}C$, $25^{\circ}C$ and 3$0^{\circ}C$ were measured. The head capsule development was slower at 2$0^{\circ}C$ on the same diets than $25^{\circ}C$ and 3$0^{\circ}C$, and slower in the flue-cured and aromatic tobacco than the artificial diet at 2$0^{\circ}C$ and 2S$^{\circ}C$, At $25^{\circ}C$ the larval head capsule developed more rapidly in the aromatic tobacco than the flue-cured tobacco. However, no larval head development was noticed in the air-cured tobacco. The correlation between population densities of cigarette beetle,1 sewicome F., and sugar content of tobacco leaves was positive, but negative in nicotine contents. The regression between densities and sugar content was Y : 22.79 X + 57.29 (r=0.891**), and between nicotine content was Y : -141.31X + 321.40(r=-0.917**).

• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.287-294
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• 2014

The transcription factor E2F1 is active during G1 to S transition and is involved in the cell cycle and progression. A recent study reported that increased E2F1 is associated with DNA damage and tumor development in several tissues using transgenic models. Here, we show that E2F1 expression is regulated by tristetraprolin (TTP) in prostate cancer. Overexpression of TTP decreased the stability of E2F1 mRNA and the expression level of E2F1. In contrast, inhibition of TTP using siRNA increased the E2F1 expression. E2F1 mRNA contains three AREs within the 3'UTR, and TTP destabilized a luciferase mRNA that contained the E2F1 mRNA 3'UTR. Analyses of point mutants of the E2F1 mRNA 3'UTR demonstrated that ARE2 was mostly responsible for the TTP-mediated destabilization of E2F1 mRNA. RNA EMSA revealed that TTP binds directly to the E2F1 mRNA 3'UTR of ARE2. Moreover, treatment with siRNA against TTP increased the proliferation of PC3 human prostate cancer cells. Taken together, these results demonstrate that E2F1 mRNA is a physiological target of TTP and suggests that TTP controls proliferation as well as migration and invasion through the regulation of E2F1 mRNA stability.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.641-645
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• 2015

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.

• 한국세라믹학회지
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• 제55권5호
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• pp.498-503
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• 2018

Using the thermal ablation method, the oxidation behavior of $SiC_f/SiC$ composites was investigated in air and in the temperature range of $1,300^{\circ}C$ to $2,000^{\circ}C$. At the relatively low temperature of $1,300^{\circ}C$, passive oxidation, which formed amorphous phase, predominantly occurred in the thermal ablation test. When the oxidation temperature increased, SiO (g) and CO (g) were formed by active oxidation and the dense oxide layer changed to a porous one by vaporization of gas phases. In the higher temperature oxidation test, both active oxidation due to $SiO_2$ decomposition on the surface of the oxide layer and active/passive oxidation transition due to interfacial reaction between oxide and base materials such as SiC fiber and matrix phase simultaneously occurred. This was another cause of high temperature degradation of $SiC_f/SiC$ composites.

• 말소리와 음성과학
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• 제8권1호
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• pp.25-36
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• 2016

Previous studies showed that the voice onset time (VOT) of aspirated and lenis stops has been merged, and post-stop fundamental frequency (F0) has emerged as a primary cue to distinguish the two stops in the younger generation and female speech. The purpose of this study is to demonstrate that VOT merger in aspirated and lenis stops occurs after an F0 difference between the two stops becomes stabilized. In other words, unless post-stop F0, which is a redundant feature, is fully developed, it is hard for VOT merger to happen. Females have got a stable F0 difference in stops earlier than males. Therefore, VOT merger could happen, and as a result, females could take the lead in changing from VOT to F0 in initial stops. This study also shows that speakers who acquired F0 as a primary cue use F0 to the full to distinguish lenis stops from two other stops (aspirated and fortis).

• 복식
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• 제24권
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• pp.235-251
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• 1995

The purpose of this study is to analyze the charateristics of foot according to foot type and analyze the change of foot shape by movements for the sake of making a comfort-able footwears. On the 6 foot type classified by F.I. and F. P. A, this study analyze the surface areas and circumference in the surface area development figure. There are selected eighteen college girls and four movements. The results are as followes : 1. In the 6 foot types classified by F.I. and F. P. A., the circumferences were differed sig-nificantly. 2. In the 6 foot types classified by F.I. and F. P. A., the surface areas were not differed siginificantly. 3. By movements, circumferences and surfaces were not significantly changed according foot type. 4. By movements, the circumference IV, V were changed variously. 5. By movements, surface area of toes, M.P. joint and ankle were changed variously.

• 한국축산식품학회지
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• 제30권6호
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• pp.923-929
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• 2010

Colostral whey prepared from colostrum (pooled from first six post-partum milkings) was heated for 10 min at $100^{\circ}C$ Heated colostral whey was incubated with 1% enzymes (protein equivalent basis) for 15, 30, 60, 90, and 120 min at $50^{\circ}C$. Papain, pepsin, trypsin, and alcalase produced different degrees of hydrolysis (DH), 10.66%, 12.42%, 10.83%, and 25.31%, respectively, at an incubation time of 120 min. The SDS-PAGE reveals that significant amounts of bovine serum albumin (BSA), ${\beta}$-lactoglobulin (${\beta}$-LG), and ${\alpha}$-lactalbumin (${\alpha}$-LA) survived papain digestion. In contrast, pepsin completely removed BSA but not ${\beta}$-LG present in heated colostral whey. Alcalase completely eliminated BSA, ${\beta}$-LG, and ${\alpha}$-LA. This differential hydrolysis was confirmed by reversed-phase HPLC analysis. Using ion-exchange chromatography, fraction-1 (F-1) was obtained from alcalase hydrolysate at a NaCl gradient concentration of 0.25 M. Reversed-phase HPLC chromatograms of alcalase F-1 showed numerous small peaks, which probably indicate that a variety of new peptides were produced. Iron content of alcalase F-1 was 28.94 ppm, which was the highest among all enzyme fractions, whereas iron content of colostral whey was 36.56 ppm. Main amino acids contained in alcalase F-1 were Thr (15.45%), Glu (14.12%), and Ser (10.39%). Therefore, alcalase can be used to generate good iron-binding peptides in heated colostral whey, and the resulting iron-binding peptides could be suitable as a value-added food ingredient for food supplements.

• 대한방사성의약품학회지
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• 제1권2호
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• pp.75-79
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• 2015

[$^{18}F$]Fluorobenzaldehyde, which is a versatile radioactive prosthetic group, can undergo reduction, reductive amination, or oxidation to be used for synthesis of diverse radiotracers. This review covers synthesis of [$^{18}F$]fluorobenzaldehyde and its conversion to secondary prosthetic groups, and also highlights its application to the development of radiotracers.