• Research in Plant Disease
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• v.25 no.4
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• pp.157-163
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• 2019

This study aimed to assess the incidence and distribution of toxigenic fungi in Korean oat. Toxigenic fungi were isolated from oat samples collected from 12 oat fields from heading to harvest in 2017 and 2018. A total of 745 fungal colonies were isolated based on morphology and identified using marker genes. About 92% of the fungal isolates were Fusarium spp. and others were Penicillium (5.9%) and Aspergillus (2.1%). Fusarium isolates comprised mostly of F. asiaticum (83.1%), followed by F. incarnatum (5.4%), F. proliferatum (3.5%), F. fujikuroi (2.8%), F. tricinctum species complex (FTSC) 11 (1.5%) and F. graminearum (1.0%). About 97% of F. asiaticum was nivalenol type, and 3-acetyl deoxynivalenol (3.2%) and 15-acetyl deoxynivalenol (0.4%) types also were found. Pathogenicity test of the selected Fusarium isolates revealed that F. asiaticum isolates have a wide range of virulence depending on the tested plants. F. graminearum and FTSC 11 isolates from blighted spikelets were the most virulent in naked oat. All Fusarium isolates (n=18) except one (FTSC 11) produced nivalenol (0.2-7.6 ㎍/g), deoxynivalenol (0.03-6.1 ㎍/g), and zearalenone (0.1-27.0 ㎍/g) on rice medium. This study is first report that F. asiaticum causes Fusarium head blight disease of oat in Korea. These findings demonstrate the dominance of F. asiaticum in oat agroecosystems as in rice, wheat and barley in Korea.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.119-125
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• 2015

Interspecific crosses between rapeseed (Brassica napus L.) and chinese cabbage (B. campestris var. pekinensis Makino) and turnip (B. rapa L.) were made in order to examine the cross possibility and morphological phenotype of $F_1$ hybrid. The growth of pollen tube of cross between rapeseed and chinese cabbage was more rapid than cross between rapeseed and turnip. Silique formation and seed setting in silique were relatively high in the cross between rapeseed and chinese cabbage. The percentage of silique set from interspecific cross between rapeseed and chinese cabbage was 90.6% and higher 23.3% than the percentage of silique from interspecific cross rapeseed and turnip. The average number of seed per silique obtained from the cross rapeseed and chinese cabbage, and rapeseed and turnip reached 15.5 and 11.6, respectively. The morphological phenotypes of $F_1$ hybrid plants obtained from seeds in the cross between rapeseed and chinese cabbage resembled rapeseed mainly, but leaf length and leaf width were increased. The size, shape and lobation of leaves of $F_1$ hybrid plants from interspecific cross between rapeseed and turnip were intermediated between their parent species, but color of leaves was dark-green.

• Korean Journal of Plant Resources
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• v.22 no.3
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• pp.266-272
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• 2009

Morphological characteristics of leaves, trichomes and acorns were investigated in 6-year-old artificial hybrids of Q. aliena ${\times}$ Q. mongolica var. crispula and Q. serrata ${\times}$ Q. mongolica var. crispula. Leaf shapes of Q. aliena ${\times}$ Q. mongolica var. crispula $F_{1}$ were obovate and resembled to that of Q. aliena. But several characters including the size of leaf and petiole and the shape of leaf base resembled to those of Q. mongolica var. crispula. In F1 hybrids, small stellate hairs distributed sparsely on the abaxial surface and their lay length was intermediate between both parents. There were no big differences on characters of nuts and cupules between both parents and $F_{1}$ hybrids. Leaf shapes of Q. serrata ${\times}$ Q. mongolica var. crispula $F_{1}$ were obovate-elliptic, and the leaf shape and leaf base and the length of petiole resembled to those of Q. mongolica var. crispula, but leaf size and serration resembled to those of Q. serrata. The number of serration in a leaf was intermediate between both parents. Small stellate hairs distributed sparsely and large single hairs were mixed on the reverse side of leaves. there were no big differences on the number and size of stellate hairs between $F_{1}$ hybrid and Q. serrata. It is able to distinguish $F_{1}$ hybrids from both parents by the size leaf size and shapes, leaf base and serration, petiole length and trichome type in the leaf.

• Korean Journal of Plant Resources
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• v.5 no.1
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• pp.49-56
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• 1992

In the preliminary antitumor screening tests of Crude Drugs and collected plants, the methanol ic extract of Forsythia Fruits in Korean marcket showed signif icant cytotoxic activity against Chinese hamster V-79 cells, but that in Japanese market did not. The former was identifid as Forsythia viridissima Lindley and latter as F.suspensa Vahl on the basis of the morphological observation. When an aqueous solution of the extract prepared from the fruits of F. viridissima (Oleaceae) was partitioned successively with n-hexane, methylene chloride, n-butanol, the cytoyoxic activitywas concetrated in the methylene chloride extract. Fractionation of the extract was made with the guidance of bioassay against V-79 cells to give cytotoxic lignans, matariresiol (1) and arctigenin (2). Their ICso values of compounds 1 and 2 were respectively $7.8{\;}\mu\textrm{g}/ml$ 1 and $1.65{\;}\mu\textrm{g}/ml$. Also, their structures were confirmed by comparison of physical and spectral data in the literature.

• Korean Journal Plant Pathology
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• v.11 no.2
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• pp.158-164
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• 1995

토마토의 엽권에서 분리한 Fusariym graminearum이 분비하는 물질은 벼 도열병균(Pyricularia oryzae)의 여러 종의 식물병원 진균에 대한 항균활성을 나타내었으며, 이러한 활성물질을 PDA에서 본 균을 배양 한 후 chloroform으로 추출하여 분리정제 하였다. HPLC에 의하여 5종류의 활성 물질을 분획하였으며, 그중 1번(F402) 화합물을 벼 도열병균(P. oryzae)을 포함한 22개 식물 병원 진균에 대하여 살균 활성범위를 조사한 결과, 이 화합물은 50$\mu\textrm{g}$/ml 농도에서 Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum은 전혀 억제하지 못하였으며, Phytophthora spp., Cladosporium fulvum, Fusarium spp., Corynespora cassicola에는 어느 정도의 활성이 있었지만 낮게 나타났고, P. oryzae, Cochliobolus miyabeanus, Alternaria solani는 100% 억제하여 활성이 높게 나타났다. 또한 장내 세균에 대한 활성을 MIC로 비교할 때 Streptococcus pyogenes, Streptococcus faecium에 대하여는 각각 12.5, 25 $\mu\textrm{g}$/ml였고 Staphylococcus aureus는 25-50$\mu\textrm{g}$/ml으로 나타났으며, Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella aerogenes, Enterobacter cloacae에서는 100$\mu\textrm{g}$/ml 이상으로 활성이 나타나지 않았다. F402를 200$\mu\textrm{g}$/ml의 농도로 직접 살포한 식물체에서의 방제효과는 벼도열병, 벼 깨씨무늬병, 보리 흰 가루병에 대하여는 80%이상이었으나, 벼 잎집무늬마름병, 오이 잿빛곰팡이병, 토마토 역병, 밀 녹병에서는 낮았다.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.73-77
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• 2020

This study was conducted to examine the vitality of Bletilla striata Rchb.f. seed treated with different concentrations sodium hypochlorite (NaOCl) for different lengths of time. This study also examined the effect of NaOCl treatment times and concentrations on swelling formation and seed germination of B. striata seed. The non-treated B. striata seed had the highest survival rate (82.7%) Treatment with more than 1.5% NaOCl negatively affected the seed survival rate, as compared to concentrations of less than 1%. The swelling formation and seed germination percentages were highest (90.1% and 94.1%, respectively) when seeds were treated with 0.5% NaOCl for 20 min. These results can be used as important basic data for the growth and restoration of B. striata and further suggest the possibility of individual restoration in habitats.

• Korean Journal of Plant Taxonomy
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• v.41 no.1
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• pp.58-65
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• 2011

Commelina benghalensis L. and Commelina diffusa Burm. f. belonging to Commelinaceae, two species previously unrecorded as Korean flora, were collected in the lowlands of Jeju Island in Korea. Commelina benghalensis of the two species is different from others of Commelinaceae in Korea by having funnelform spathes fused at the proximal margin of involucral bracts and cleistogamous flowers. In addition, Commelina diffusa differ from others by having a three-valve capsule and lanceolate and a base cordate or rounded spathelike involucral bracts. The somatic chromosome number of Commelina benghalensis was 2n = 2x = 22 and the sizes of chromosomes were very small, ranging from 1.25 to 2.70 ${\mu}m$. However, the Commelina diffusa chromosome number exceeded 2n = ca. 100, and a precise count could not be obtained. These species are known to be distributed in tropical and subtropical regions of Asia and Africa below the latitude of Jeju Island of Korea.

• Korean journal of applied entomology
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• v.61 no.2
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• pp.319-330
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• 2022

Andong is a place to culture the great amount of hot pepper (Capsicum annuum L.) in Korea. This study reports a yearly occurrence (March 31~October 25, 2021) of thrips infesting the hot pepper in Andong. Thrips caught to yellow sticky traps were diagnosed by morphological characters and showed two dominant species: Frankliniella occidentalis and F. intonsa. During this period, a total of 107,874 thrips were caught and included F. occidentalis at about 82%, F. intonsa at about 17%, and the other thrips at about 0.3%. There were two main peaks at May~June and at September~October, respectively, in which the total number of thrips was higher in the second peak and most were F. occidentalis. Interestinly, a low level of thrips occurred during July~August was observed and explained by their susceptibility to high temperatures. A laboratory experiment by exposing thrips to high temperatures showed that thrips were susceptible to temperatures higher than 35℃ and not tolerant to 45℃ for 1 h. Indeed, high temperatures higher than 45℃ were recorded in the greenhouses in Andong during July~August. F. occidentalis was more tolerant to the high temperatures than F. intonsa. On the other hand, the thrips showed the highest occurrence peak at July~August in hot pepper-culturing greenhouse in Kangwon, where the average temperatures were mostly lower than those of Andong and no high temperatures higher than 45℃ were recorded during July~August. A viral disease caused by tomato spotted wilt virus (TSWV) was observed in the hot peppers cultured in Andong greenhouses. Multiplex PCR was used to detect the virus along with identification of thrips. With a high record of about 30%, the virus-infected thrips were detected during all the monitoring period. The virulent thrips were identified to be only F. intonsa. These results suggest that F. occidentalis gives a direct damage especially during harvesting period with their high populations while F. intonsa gives indirect damage by transmitting TSWV.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.239-244
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• 2015

Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance - POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter ($205.8{\mu}m$), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter $14.6{\mu}m$, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.77-87
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• 1994

The isolation and structure elucidation Of five new oligo-nicotinated sesquiterpene polyesters with a dihydroagarofuran core from Maytenus ilicifolia, named cangorins F, G,H,1 and J [1-5], is described. The strurtures of these compounds, which possessed nicotinyl,benroyl, and areryl groups as esterifying moieties, were elucidaied by $^IH- and ^{13}C$-nmrspectroscopic studies, inrlusive of hereronuclear correlation, long-range correlation, and nOespectra, along with mass and cd spectral dara, and the X-ray crystallographic analysis of 1.