• 임상간호연구
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• 제26권3호
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• pp.327-336
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• 2020

Purpose: The purpose of this study was to describe changes in positive psychological capital, organizational commitment and burnout according to work periods for new nurses. Methods: A longitudinal study was done using surveys of 91 nurses from a tertiary hospital located in Seoul, South Korea. Data were collected from these newly graduated nurses during the period from July 2018 to September 2019, three times (2 months, 4 months and 6 months after starting to work). Results: Hope of positive psychological capital decreased after 6 months of work (F=7.11, p=.001) and organizational commitment decreased after 4 months of work (F=15.30, p<.001). Factors influencing participants' burnout included the optimism of positive psychological capital and continuance of organizational commitment. These factors explained 51.3% of the variance in burnout (F=48.47, p<.001). Conclusion: Organizational support for new nurses is urgently needed. It is necessary to develop programs to strengthen the positive psychological capital of newly graduated nurses to reduce their burnout and to help their job adaptation.

• 한국동물위생학회지
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• 제46권4호
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• pp.303-314
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• 2023

Protective efficacy of trivalent Salmonella inactivated vaccine containing Chlorhexidine-inactivated S. Enterltidis (SE), S. Typhimurium (ST), and S. Gallinarum (SG) strains, was evaluated in this study. A total of 70 brown nick layers were divided into 7 groups, A to G, containing 10 hens per group. All hens in groups B to D were intramuscularly immunized with approximately 7×10⁸ cells (3×10⁸ cells of SE+1×10⁸ SE+1×10⁸ cells of ST+3×10⁸ cells of SG) of the trivalent vaccine in 0.5 mL of PBS. All chickens in groups E to G were injected with sterile PBS. All hens of groups B and E, groups C and F, and groups D and G were orally challenged with approximately 2 ×10⁹ CFU of wild-type SE, ST, and SG, respectively. Serum IgG titers and CD3⁺CD4⁺ T-cells, and CD3⁺CD8⁺ T-cells levels of groups B to D significantly higher than those of group A. In addition, all animals in groups A to C, E and F showed no clinical symptoms and survived after the virulent challenges, whereas one chicken in group D died and all chickens in group G died following the challenge. The protection against wild-type SE and ST in liver, spleen, cecum, and cloaca of groups B and C chickens was significant effective as compared with those in groups E and F. These indicate that the trivalent inactivated vaccine can be an effective tool for prevention of Salmonella infections by inducing robustly protective immune responses and cellular immune response in chickens.

• 농업생명과학연구
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• 제45권2호
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• pp.43-50
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• 2011

본 시험은 무궁화과에 속하는 초본 식물인 Kenaf (Hibiscus cannabinus L.)를 경상대학교 시험농장에서 2010년 6월 1일 파종하여 같은 해 11월 18일 수확하여 건조 한 후, 꽃 (HCME-F)과 잎 (HCME-L)에서 추출한 메탄올 추출물을 이용하여, 세포 독성 시험 및 주요 병원균에 대한 항균효과를 규명하였다. 항균효과에 사용된 균주는 가축에서 피부 질환, 유방염 및 소화기질병을 유발하는 그람양성균인 St. aureus 와 Str. epidermidis, 그람음성균인 S. typhimurium 과 E. coli 균을 공시균주로 사용하였다. 추출물의 안전성을 평가하기 위해 두 추출물 HCME-F과 HCME-L을 0, 25, 50, 100 및 $200{\mu}g/ml$ 농도로 첨가한 배지에서 RAW 264.7 세포와 24시간 반응 후 세포 독성을 측정해 본 결과, 세포 독성이 인정되지 않았으며, 항균효과 시험결과 그람양성균인 St. aureus와 Str. epidermidis 균에 대하여 1, 50 및 $100{\mu}g/ml$의 추출물 농도 및 반응시간 경과에 따라 항균 효과가 증가되었으나, 그람음성균인 S. typhimurium 과 E. coli 에서는 항균효과가 인정되지 않았다. 종합적으로 Kenaf의 꽃과 잎에서 추출한 메탄올 추출물이 세포에 대한 안전성이 입증되었고, 가축과 사람의 피부 질환 및 유방염의 대표적 그람양성균인 St. aureus와 Str. epidermidis 균에 대한 항균효과를 보여, Kenaf의 꽃과 잎을 이용한 선택적 그람양성균 치료제 및 사료첨가제 개발이 가능할 것으로 판단된다.

• 천문학회보
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• 제29권2호
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• pp.41.1-41.1
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• 2004

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 1994년도 교수 연수회(역학)
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• pp.196-200
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• 1994

• 한국도로학회:학술대회논문집
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• 한국도로학회 1999년도 학술발표회 논문집
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• pp.3-28
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• 1999

• Animal cells and systems
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• 제15권4호
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• pp.259-267
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• 2011

This study was undertaken to produce a $F_{ab}$ fragment of a human monoclonal antibody reactive to oxidized and carbamylated low-density lipoprotein (oxLDL and cLDL) using phage display technology. An analysis of DNA sequences of this $F_{ab}$, termed plaque 15,16-46 $F_{ab}$, revealed that the rearranged $V_H$ was highly mutated. Complementarity-determining regions of the $V_H$ showed a very high R/S ratio and contained many positively charged amino acids. In direct binding and competitive ELISA, the $F_{ab}$ reacted strongly with both MDA-LDL and Cu-oxLDL forms of oxLDL, and also showed high affinity for cLDL. Immunofluorescence and immunohistochemical analyses showed that this $F_{ab}$ positively stained atherosclerotic aortic plaques in $ApoE^{-/-}$ mice as well as those in patients with atherosclerosis. The $F_{ab}$ also showed positive staining in placental decidua from patients with preeclampsia. It is suggested that the plaque 15,16-46 $F_{ab}$ against oxLDL and cLDL might possibly be applicable for developing a diagnostic reagent for both human and rodent animal research to detect and characterize atherosclerotic disease progression in atherosclerotic lesions as well as exploring the pathogenesis of atherogenic diseases such as preeclampsia.

• Journal of Microbiology
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• 제39권3호
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• pp.236-239
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• 2001

Group A Streptococcus strain ST4529 is a provisional new ems type which has been recently reported in Malaysia (Jomal, et al. 1999. Energ. Infect . Dis. 5,10-14). This strain was found to be opacity factor (OF) negative with a Tl phenotype. Usually, OF negative strains with T1 phenotypes are associated with acute rheumatic fever. However, strain ST4529 was isolated from the blood of a patient with septicemia. Comparison of the deduced amino acid sequence of the mature hypervariable N-terminus of ST4529 showed only 43% identity with that of M5, the closest matched OF negative strain with a T1 phenotype. Thus, ST4529 most probably encodes a new serospecifically unique M protein which is associated with septicemia rather than pharyngitis infections. The strains with these phenotypes are very important because their sequences should be considered for developing any anti-streptococcal vaccines.

• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2011년도 제44차 학술발표회
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• pp.94-94
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• 2011

스포츠웨어 바람막이용도에 사용되는 대부분의 소재는 폴리아미드계 소재이며, 국내에서는 대부분 나일론6 소재를 사용하고 있다. 최근 소비자들의 요구는 점점 경량화되고 있고 야외활동 증가와 더불어 착용감이 우수한 소재를 요구하고 있다. 이러한 시즘에 국내에서 많이 사용되는 나일론6 소재를 이용하여 직물단계에서 경량감과 스트레치성이 발현될수 있는 나일론6 세섬 권축사 개발에 대한 연구를 진행하고자 함이다. 본 연구에서는 직물단계에서 경량감과 스트레치성이 발현될수 있는 나일론6 세섬 권축사 개발을 위해 공중합 나일론6 폴리머와 일반 나일론6 폴리머를 복합방사설비를 이용하여 SIDE BY SIDE POY 26d/6f 원사를 제조하였다. 원사물성은 섬도 25.8d, 절단신도 71%, 절단강도 4.3g/d의 물성을 가졌으며, Nip Belt 가연설비로 연신비, 가연 1st 히터온도, 벨트각도 조건에 따라서 제조된 나일론6 가연사 DTY 20d/6f의 물성을 평가하였고, 비교사로서 T사에서 생산되고 있는 나일론6 가연사 DTY 20d/6f와 비교평가하였다. 비수탄성율은 가연 연신비가 높고, 1st 히터온도가 높은 조건에서 높게 나타남을 알 수 있었으며, 염색 가공 공정에서의 전처리단계에서 $100^{\circ}C$정도의 열을 받았을 때의 수축에 따른 권축효과 발현에 따른 신축성 변화에 대한 상대평가를 사단계에서 유추할 수 있다. 권축효과가 높게 나타난다 하더라도, 사의 수축힘이 적으면 권축효과 발현특성에 큰 차이가 없으므로 열응력을 측정을 한 결과, 가연 연신비가 높고 가연 1st 히터온도가 높은 조건에서 열응력이 높게 나타남을 알 수 있었으며, 비교사에 비해 약간 높은 열응력 값을 보였다. 절단강도는 가연조건에 따라서 큰 변화가 없었고, 절단신도는 연신비가 증가함에 따라 점차 감소하였으며 비교사와도 큰 차이를 보이지 않았음. 따라서, POY 26d/6f 원사에 적합하고, 권축효과가 우수한 가연조건을 도출하였고, 가연현장 작업시 문제가 없는 가연 최적조건을 도출하였다. 향후, 가연조건에 따른 제조된 가연사를 이용하여 제직 및 염색가공 공정을 거친후, 직물신축성 평가를 실시할 계획이다.

• Radiation Oncology Journal
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• 제35권4호
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• pp.306-316
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• 2017

Purpose: To investigate the predictive role of maximum standardized uptake value ($SUV_{max}$) of 2-[$^{18}F$]fluoro-2-deoxy-D-glucose($^{18}F-FDG$) positron emission tomography/computed tomography (PET/CT) in nasopharyngeal cancer patients treated with intensity-modulated radiotherapy (IMRT). Materials and Methods: Between October 2006 and April 2016, 53 patients were treated with IMRT in two institutions and their PET/CT at the time of diagnosis was reviewed. The $SUV_{max}$ of their nasopharyngeal lesions and metastatic lymph nodes (LN) was recorded. IMRT was delivered using helical tomotherapy. All patients except for one were treated with concurrent chemoradiation therapy (CCRT). Correlations between $SUV_{max}$ and patients' survival and recurrence were analyzed. Results: At a median follow-up time of 31.5 months (range, 3.4 to 98.7 months), the 3-year overall survival (OS) and disease-free survival (DFS) rates were 83.2% and 77.5%, respectively. In univariate analysis, patients with a higher nodal pre-treatment $SUV_{max}$ (${\geq}13.4$) demonstrated significantly lower 3-year OS (93.1% vs. 55.5%; p = 0.003), DFS (92.7% vs. 38.5%; p < 0.001), locoregional recurrence-free survival (100% vs. 50.5%; p < 0.001), and distant metastasis-free survival (100% vs. 69.2%; p = 0.004), respectively. In multivariate analysis, high pre-treatment nodal $SUV_{max}$ (${\geq}13.4$) was a negative prognostic factor for OS (hazard ratio [HR], 7.799; 95% confidence interval [CI], 1.506-40.397; p = 0.014) and DFS (HR, 9.392; 95% CI, 1.989-44.339; p = 0.005). Conclusions: High pre-treatment nodal $SUV_{max}$ was an independent prognosticator of survival and disease progression in nasopharyngeal carcinoma patients treated with IMRT in our cohort. Therefore, nodal $SUV_{max}$ may provide important information for identifying patients who require more aggressive treatment.