• BMB Reports
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• 제37권2호
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• pp.199-205
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• 2004

The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fibrinolytic activities of the six fractions on the fibrin plates ranged from 20.8 to 207.2 unit/mg with rank orders of F6 > F2 > F5 > F3 > F1 > F4. The molecular weights of each iso-enzyme, as estimated by SDS-PAGE, were 24.6 (F1), 26.8 (F2), 28.2 (F3), 25.4 (F4), 33.1 (F5), and 33.0 kDa (F6), respectively. The plasminogen was activated into plasmin by the enzymes. The optimal temperature of the six iso-enzymes was $50^{\circ}C$, and the optimal pH ranged from pH 4-12. The four iso-enzymes (F1-F4) were completely inhibited by PMSF. The two enzymes (F5 and F6) were completely inhibited by aprotinin, TLCK, TPCK, SBTI, LBTI, and leupeptin. The N-terminal amino acid (aa) sequences of the first 20 to 22 residues of each fraction had high homology. All six isoenzymes had identical aa residues 2-3 and 13-15. The N-terminal 21-22 aa sequences of the F2, F3, and F4 isoenzymes were almost the same. The N-terminal aa sequences of F5 and F6 were identical.

• Clinical and Experimental Reproductive Medicine
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• 제20권2호
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• pp.157-160
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• 1993

The human follicular f1uids(F.F.) may be considered to contribute the maturation of the oocytes on the in vitro culture. To investigate the effects of epidermal growth factor (E.G.F.), which is present in mature and immature follicular fluids, we had experiments of mouse oocytes maturation in vitro. The endpoints assayed were rated as percentage of oocytes undergoing germinal vesicle breakdown(G.V.B.D.) and polar body(P.B.) formation at 12 hours after in vitro culture. The rates of G.B.B.D. were 87% in mature F.F. 68% in immature F.F. and 78% in Ham's F-10 medium respectively. And overall the mature F.F. seem to stimulate on in vitro oocyte maturation compared with either immature F.F. or Ham's F-10 medium. As the concentration of addition of E.G.F. in immature F.F., the rates of G.V.B.D. and P.B. formation were 82 %, 23% in addition with 2 ng/ml while 84%, 32% in addition with 4 ng/ml respectivly. And at the concentration of addition of E.G.F. in Ham's F-10 media as well, the rates of G.V.B.D. and P.B. formation were 84%, 40% and 82%, 44% in addition with each 2ng, 4ng. AccordinglY there was no influence on the oocytes maturation at the addition of E.G.F. to each immature F.F. and Ham's F-10 medium. In conclusion, the E.G.F. is not able to induce oocyte maturation independent of it's effects in immature F.F. and Ham's F-10 media.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제24권2호
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• pp.109-127
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• 2017

In this paper, we solve the following quadratic ${\rho}-functional$ inequalities ${\parallel}f({\frac{x+y+z}{2}})+f({\frac{x-y-z}{2}})+f({\frac{y-x-z}{2}})+f({\frac{z-x-y}{2}})-f(x)-f(y)f(z){\parallel}$ (0.1) ${\leq}{\parallel}{\rho}(f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z)){\parallel}$, where ${\rho}$ is a fixed non-Archimedean number with ${\mid}{\rho}{\mid}$ < ${\frac{1}{{\mid}4{\mid}}}$, and ${\parallel}f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z){\parallel}$ (0.2) ${\leq}{\parallel}{\rho}(f({\frac{x+y+z}{2}})+f({\frac{x-y-z}{2}})+f({\frac{y-x-z}{2}})+f({\frac{z-x-y}{2}})-f(x)-f(y)f(z)){\parallel}$, where ${\rho}$ is a fixed non-Archimedean number with ${\mid}{\rho}{\mid}$ < ${\mid}8{\mid}$. Using the direct method, we prove the Hyers-Ulam stability of the quadratic ${\rho}-functional$ inequalities (0.1) and (0.2) in non-Archimedean Banach spaces and prove the Hyers-Ulam stability of quadratic ${\rho}-functional$ equations associated with the quadratic ${\rho}-functional$ inequalities (0.1) and (0.2) in non-Archimedean Banach spaces.

• 식물병연구
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• 제29권2호
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• pp.118-129
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• 2023

Fusarium sambucinum 종 복합체는 식물에 심각한 질병을 일으키는 분류군이다. 국내에서는 F. sambucinum 종 복합체가 일으키는 식물병에 대한 많은 연구가 보고되었으나, 한국식물 병명목록에는 2종(F. graminearum, F. sambucinum)이 14개의 기주에 식물병을 일으키는 정보만이 등록되어 있다. F. sambucinum 종복합체의 다양성 및 병원성을 확인하기 위해 농업미생물은행(Korean Agricultural Culture Collection)에 보존된 57 균주의 F. sambucinum 종 복합체를 다중 유전자 염기서열 분석(multi-locus sequence typing)을 바탕으로 7종(F. asiaticum, F. graminearum, F. vorosii, F. meridionale, F. bootii, F. kyushuense, F. armeniacum)으로 재동정하였다. 이전의 보고와 이번 연구의 결과에 따라, F. sambucinum 종 복합체 중 5종(F. asiatum, F. graminearum, F. vorosii, F. armeniacum, F. sambucinum)이 24개의 기주에 병원성을 보였으며, 3종(F. meridionale, F. bootii, F. kyushuense)의 병원성은 명확하지 않았다.

• 호남수학학술지
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• 제1권1호
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• pp.21-25
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• 1979

Abelian군(群)의 presheaf에 관한 직적(直積)과 직화(直和)를 Category 입장에서 정의(定義)하고 presheaf $F_{\lambda}\;({\lambda}{\epsilon}{\Lambda})$들의 두 직적(直積)(또는 直和)은 서로 동형적(同型的) 관계(關係)에 있으며, 특히 ${\phi}:X{\rightarrow}Y$가 homeomorphism이라 하고 ${\phi}_*F$를 X상(上)의 presheaf F의 direct image이라 하면 (1) $({\phi}_*F, \;{\phi}_*(f_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$가 $({\phi}_*F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}$의 직적(直積)일 때 오직 그때 한하여 $(F,\;(f_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$는 $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$의 직적(直積)이다. (2) $({\phi}_*F,\;{\phi}_*(l_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$가 $({\phi}_*F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}$의 직화(直和)일 때 오직 그때 한하여 $(F,\;(l_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$는 $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$의 직화(直和)이다. Let $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$ be an indexed set of presheaves of abelian group on topological space X. We can define the cartesian product $$\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda}$$ of $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$ by $$(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(U)=\prod_{{\lambda}{\epsilon}{\Lambda}}(F_{\lambda}(U))$$ for U open in X $${\rho}_v^u:\;(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(U){\rightarrow}(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(V)((s_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}{\rightarrow}(_{\lambda}{\rho}_v^u(s_{\lambda}))_{{\lambda}{\epsilon}{\Lambda}})$$ for $V{\subseteq}U$ open in X where $_{\lambda}{\rho}^U_V$ is a restriction of $F_{\lambda}$, And we have natural presheaf morphisms ${\pi}_{\lambda}$ and ${\iota}_{\lambda}$ such that ${\pi}_{\lambda}(U):\;({\prod}_\;F_{\lambda})(U){\rightarrow}F_{\lambda}(U)((s_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}{\rightarrow}s_{\lambda})$ ${\iota}_{\lambda}(U):\;F_{\lambda}(U){\rightarrow}({\prod}\;F_{\lambda})(U)(s_{\lambda}{\rightarrow}(o,o,{\cdots}\;{\cdots}o,s_{\lambda},o,{\cdots}\;{\cdots}o)$ for $(s_{\lambda}){\epsilon}{\prod}_{\lambda}\;F_{\lambda}(U)$ and $(s_{\lambda}){\epsilon}F_{\lambda}(U)$.

• Mycobiology
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• 제30권3호
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• pp.139-145
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• 2002

Gibberella fujikuroi is species complex. This species complex includes Fusarium tabacinum, F. moniliforme(=F. verticillioides), F. nygamai, F. proliferatum as well as F. subglutinans. Our objective was to develop a technique to differentiate between isolates of F. subglutinans, F. proliferatum and F. verticillioides. Thirty-two strains of F. subglutinans, six strains from F. verticillioides and five strains of F. Proliferatum isolated from maize in Austria were studied using random amplified polymorphic DNA(RAPD). F. subglutinans strains clustered very closely, with similarity ranging from $87{\sim}100%$. On the other hand, all the amplification patterns of F. verticillioides were identical, as well as in the case of F. proliferatum. Our results indicated that these Fusaria species are distinct species and hence RAPD markers can be quick and reliable for differentiating them.

• Journal of applied mathematics & informatics
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• 제19권1_2호
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• pp.127-133
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• 2005

An f-coloring of a graph G = (V, E) is a coloring of edge set E such that each color appears at each vertex v $\in$ V at most f(v) times. The minimum number of colors needed to f-color G is called the f-chromatic index $\chi'_f(G)$ of G. Any graph G has f-chromatic index equal to ${\Delta}_f(G)\;or\;{\Delta}_f(G)+1,\;where\;{\Delta}_f(G)\;=\;max\{{\lceil}\frac{d(v)}{f(v)}{\rceil}\}$. If $\chi'_f(G)$= ${\Delta}$f(G), then G is of $C_f$ 1 ; otherwise G is of $C_f$ 2. In this paper, the classification problem of complete graphs on f-coloring is solved completely.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제23권3호
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• pp.247-263
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• 2016

Let $M_{1}f(x,y):=\frac{3}{4}f(x+y)-\frac{1}{4}f(-x-y)+\frac{1}{4}f(x-y)+\frac{1}{4}f(y-x)-f(x)-f(y)$, $M_{2}f(x,y):=2f(\frac{x+y}{2})+f(\frac{x-y}{2})+f(\frac{y-x}{2})-f(x)-f(y)$. Using the direct method, we prove the Hyers-Ulam stability of the additive-quadratic ρ-functional inequalities (0.1) $N(M_{1}f(x,y),t){\geq}N({\rho}M_{2}f(x,y),t)$ where ρ is a fixed real number with |ρ| < 1, and (0.2) $N(M_{2}f(x,y),t){\geq}N({\rho}M_{1}f(x,y),t)$ where ρ is a fixed real number with |ρ| < $\frac{1}{2}$.

• Applied Biological Chemistry
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• 제49권1호
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• pp.25-29
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• 2006

하귤(Citrus natsudaidai Hayata)의 과피를 cold-press하여 얻어진 정유성분을 column chromatography, HPLC 및 TLC로 분리 정제하여 각 분획에 대하여 EBV활성화에 대한 억제효과를 측정하였다. Column chromatography로 정유성분의 분리를 시도하여 7개 의 peak$(F-I{\sim}VII)$를 얻을 수 있었다. 이것들을 TLC로 분석한 결과, 각각 단일 반점만을 나타냈으며 $R_f$값은 0.31, 0.13, 0.13 0.78, 0.79, 0.69, 0.84이었다. 7개의 peak중에서 F-I, II 및 F-IV 등 3개의 peak에 대하여 HPLC분석을 한 결과 F-I 및 F-II는 단일 peak이며 retention time은 F-I이 3분, F-II는 2.5분이었다. 그러나 F-IV는 2개의 peak가 나타났으며 retention time은 각각 2분과 4.5분이었다. 4.5분에 나타난 peak는 극히 적은 면적으로 보아 극소량의 물질이어서 앞서의 TLC분석에서 나타나지 많았던 것으로 생각된다. $F-I{\sim}F-VII$에 대한 EBV활성화에 대한 억제효과 측정은 Raji cells을 이용하여 간접형광 항체법으로 실시했다. 억제율은 F-VI가 $82.3{\pm}1.3%$으로 가장 높았으며 다음이 F-I$(80.4{\pm}1.6%)$ > F-II$(77.2{\pm}0.9%)$ > F-III$(75.0{\pm}1.2%)$ > F-IV$(74.1{\pm}1.0.%)$ > F-V$(71.0{\pm}1.1%)$ > F-VII$(70.2{\pm}1.2%)$의 순으로 모두 70% 이상의 억제율을 나타냈다. 이상의 결과는 하귤과피에는 발암 promotion 억제활성성분이 존재한다는 것을 시사한 것이다.

• 호남수학학술지
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• 제37권4호
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• pp.473-486
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• 2015

In this paper, we investigate a stability problem for a functional equation f(-x - y - z) - f(x + y) - f(y + z) - f(x + z) + 2f(x) + 2f(y) + 2f(z) - f(-x) - f(-y) - f(-z) = 0 by applying the direct method.