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Genetic assessment of BoLA-DRB3 polymorphisms by comparing Bangladesh, Ethiopian, and Korean cattle

  • Mandefro, Ayele;Sisay, Tesfaye;Edea, Zewdu;Uzzaman, Md. Rasel;Kim, Kwan-Suk;Dadi, Hailu
    • Journal of Animal Science and Technology
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    • v.63 no.2
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    • pp.248-261
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    • 2021
  • Attributable to their major function in pathogen recognition, the use of bovine leukocyte antigens (BoLA) as disease markers in immunological traits in cattle is well established. However, limited report exists on polymorphism of the BoLA gene in zebu cattle breeds by high resolution typing methods. Thus, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 100 animals (Boran, n = 13; Sheko, n = 20; Fogera, n = 16; Horro, n = 19), Hanwoo cattle (n = 18) and Bangladesh Red Chittagong zebu (n = 14). Out of the 59 detected alleles, 43 were already deposited under the Immuno Polymorphism Database for major histocompatibility complex (IPD-MHC) while 16 were unique to this study. Assessment of the level of genetic variability at the population and sequence levels with genetic distance in the breeds considered in this study showed that Zebu breeds had a gene diversity score greater than 0.752, nucleotide diversity score greater than 0.152, and mean number of pairwise differences higher than 14, being very comparable to those investigated for other cattle breeds. Regarding neutrality tests analyzed, we investigated that all the breeds except Hanwoo had an excess number of alleles and could be expected from a recent population expansion or genetic hitchhiking. Howbeit, the observed heterozygosity was not significantly (p < 0.05) higher than the expected heterozygosity. The Hardy Weinberg equilibrium (HWE) analysis revealed non-significant excess of heterozygote animals, indicative of plausible over-dominant selection. The pairwise FST values suggested a low genetic variation among all the breeds (FST = 0.056; p < 0.05), besides the rooting from the evolutionary or domestication history of the cattle. No detached clade was observed in the evolutionary divergence study of the BoLA-DRB3 gene, inferred from the phylogenetic tree based on the maximum likelihood model. The investigation herein indicated the clear differences in BoLA-DRB3 gene variability between African and Asian cattle breeds.

A Study on Value Orientation of Health of Rural Health Center Milieu (농촌지역 보건소 환경에 있어서의 건강가치관에 관한 일 조사연구)

  • 김순자
    • Journal of Korean Academy of Nursing
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    • v.5 no.1
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    • pp.17-30
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    • 1975
  • The concept and definition of nursing and her role have been changing in accordance with the socio-cultural factors of the initial society. At present, nursing is conceptualized as a health care profession assisting man to restore, maintain and promote health by providing knowledge, wilt strength and resources through various processes of interaction. Man′s behavior, of individual and group activities for health inclusive, is driven by the initial man′s value orientation. The purpose of this study is to investigate the value orientation regarding health of rural health center milieu in order to give data for; 1. the planning for the delivery of community nursing service, 2. the health education plan at all level of nursing care activities, and 3. the planning of curriculum for nursing education. A hundred opinion leaders among the labor population residing in rural areas (P-group), hundred and six professional nurses at rural health centers (N-group) were indirectly interviewed through questionaries. And ninety five of N-group were interviewed likewise of their perceptions of P-group (NP-group) from July 15, to October 15, 1974. The result is as follows: 1. Maintenance of health is revealed to be the most valued component for man′s happiness in all the three groups. (P-group: 7.30 S. D.=1.31), (N-group :7.84 S. D. =49), and (NP-group : 5.93 S. D. =2.28) 2. The average value score of the maintenance of health revealed significant difference by P〈.001 level between each of the three groups. (Between N-group and P-group : T= -4.07 P and NP ; T=-6.93, N and NP: T=-9.35) 3. Basic health maintenance activities necessary for maintenance and promotion of personal health were moderately valued by all the three groups, P-group ; 3.74 (S. D. =.43) , N-group: 3.52 (S. D. =.34), NP-group: 3.07 (S. D. :.55) Among the 8 categories of basic health maintenance activities, "food intake" was highly valued by P-group (mean value score; 4.00 S. D=.51) , "exercise and rest" and "personal cleanliness" was highly valued by both P-group (4.02, 4.08) and N-group (4.08, 4.22). 4. The mean value score of basic health maintenance activities revealed significant difference by P〈.001 level between each of the three groups (between P-group and N-group: T=-4, 07, N-and NP: T=-6.93, P and NP T=-9.35) 5. Among the 30 questionaries, dynamic activities for health maintenance were more valued in comparison to passive activities in an tile three groups. 6. In N-group, correlation between the value of basic health maintenance activities and the personal health status personal revealed moderate significance. Correlation between the value of basic health maintenance activities and the age revealed low significance. 7. In group, correlation between the value of basic health maintenance activities and; perception of personal health status revealed non significance, between the age and sex revealed low sign affiance, and between the educational status revealed moderate significance. Recommendations are as follows ; 1. The efforts towards alteration of value orientation concerning health in general in community nursing practice de focussed be that of family Planning. 2. In order to prepare professional nurses competent in understanding individual and group, social science and behavioral science be strength ended in planning nursing curriculum. 3. Milieu of nursing experience during nursing education be Planned to begin at simple nursing problem and move towards complex, f. e. home care towards health crisis situation in order to achieve dynamic role mastery.

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Effect of Postmortem Metabolic Rate on Meat Color

  • Park, B.Y.;Lee, J.M.;Hwang, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.598-604
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    • 2007
  • The current study was conducted to evaluate carcass characteristics, objectives and sensory meat qualities of Hanwoo longissimus muscle as affected by ultimate pH. Twenty-four steers and bulls ($556{\pm}53$ kg and $0.63{\pm}0.32$cm for live weight and backfat thickness, respectively) were used. As there was a linear relationship (r = 0.77) between lean meat color and ultimate pH, cattles were thus segregated into normal $pH{\leq}5.8$, Normal, n = 13) and DFD (pH>5.8, n = 11) groups. Normal pH group had significantly (p<0.05) higher carcass weight, marbling score and backfat thickness than those for high pH group, while fat color and lean meat color were inverse. In principle component analysis for co-ordinates of DFD and normal meats, fat color, lean meat color, texture, time to pH 6.2 and pH at 24 h postmortem were associated with the positive range of the first factor (67.5%) while backfat thickness marbling score and temperature at 24 h were placed in negative values. The rate constant of decline in pH (pH k) did not differ between the two groups, implying that initial pH (i.e., pH at slaughtering) differed between two groups. Contour mapping of pH k between pH at 1 and 24 h postmortem indicated that high pH k was related to lower pHs at 1 and 24 h postmortem. This suggested that the high pH cattles (i.e., DFD cattle) resulted from their own potential. Although the time to reach pH 6.2 was significantly (p<0.05) shorter for normal meat (i.e., 3.2 h) than that for DFD one (i.e., 19.8 h), there were no significantly differences in both WB-shear force and sensory attributes. Given that the experimental animals were sampled from a similar group, which implies a similar myoglobin pigment content, the current data suggested that pre- and post-slaughter animal handling likely had a significant effect on ultimate pH and consequently meat color of Hanwoo longissimus muscle, and also small animals with lower marbling score and backfat thickness had a higher risk for DFD meat.

E-learning in India and Sri Lanka: A Cross-Cultural Study

  • Simmy Kurian;Hareesh N. Ramanathan;Chamaru De Alwis
    • Asia pacific journal of information systems
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    • v.31 no.1
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    • pp.102-120
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    • 2021
  • E-learning is a planned effort towards providing interactive and experiential learning having flexibility in terms of time, place, pace, participation and accessibility. Globalization has set the stage for a social transformation of the world economy driven by technological innovation, emphasizing knowledge-based processes. While the tertiary education enrolments in wealthy nations have gone up incrementally, the same cannot be said to be right about developing economies. E-Learning can streamline enrolments to higher education, in developing nations by being a cost-effective and flexible alternative. The objective of this paper is to draw attention to the similarities in the national culture of these two countries and compare students' perception on e-learning in India and Sri Lanka along eight dimensions viz., viability, dependability, flexibility, inclusivity, power, pertinence, challenge and equitability. The results reveal that e-learning is equally popular among students from both countries, and they have a high perception score towards e-learning on each of the measured eight dimensions. Hence results are indicative of an opportunity of tapping the potential of e-learning in reaching out to a broader audience of underprivileged students and onboarding them into the knowledge economy.

Expression Profiles of Loneliness-associated Genes for Survival Prediction in Cancer Patients

  • You, Liang-Fu;Yeh, Jia-Rong;Su, Mu-Chun
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.1
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    • pp.185-190
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    • 2014
  • Influence of loneliness on human survival has been established epidemiologically, but genomic research remains undeveloped. We identified 34 loneliness-associated genes which were statistically significant for high-lonely and low-lonely individuals. With the univariate Cox proportional hazards regression model, we obtained corresponding regression coefficients for loneliness-associated genes fo individual cancer patients. Furthermore, risk scores could be generated with the combination of gene expression level multiplied by corresponding regression coefficients of loneliness-associated genes. We verified that high-risk score cancer patients had shorter mean survival time than their low-risk score counterparts. Then we validated the loneliness-associated gene signature in three independent brain cancer cohorts with Kaplan-Meier survival curves (n=77, 85 and 191), significantly separable by log-rank test with hazard ratios (HR) >1 and p-values <0.0001 (HR=2.94, 3.82, and 1.78). Moreover, we validated the loneliness-associated gene signature in bone cancer (HR=5.10, p-value=4.69e-3), lung cancer (HR=2.86, p-value=4.71e-5), ovarian cancer (HR=1.97, p-value=3.11e-5), and leukemia (HR=2.06, p-value=1.79e-4) cohorts. The last lymphoma cohort proved to have an HR=3.50, p-value=1.15e-7. Loneliness-associated genes had good survival prediction for cancer patients, especially bone cancer patients. Our study provided the first indication that expression of loneliness-associated genes are related to survival time of cancer patients.

Research on Transformer-Based Approaches for MBTI Classification Using Social Network Service Data (트랜스포머 기반 MBTI 성격 유형 분류 연구 : 소셜 네트워크 서비스 데이터를 중심으로)

  • Jae-Joon Jung;Heui-Seok Lim
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2023.07a
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    • pp.529-532
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    • 2023
  • 본 논문은 소셜 네트워크 이용자의 텍스트 데이터를 대상으로, 트랜스포머 계열의 언어모델을 전이학습해 이용자의 MBTI 성격 유형을 분류한 국내 첫 연구이다. Kaggle MBTI Dataset을 대상으로 RoBERTa Distill, DeBERTa-V3 등의 사전 학습모델로 전이학습을 해, MBTI E/I, N/S, T/F, J/P 네 유형에 대한 분류의 평균 정확도는 87.9181, 평균 F-1 Score는 87.58를 도출했다. 해외 연구의 State-of-the-art보다 네 유형에 대한 F1-Score 표준편차를 50.1% 낮춰, 유형별 더 고른 분류 성과를 보였다. 또, Twitter, Reddit과 같은 글로벌 소셜 네트워크 서비스의 텍스트 데이터를 추가로 분류, 트랜스포머 기반의 MBTI 분류 방법론을 확장했다.

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Factors Affecting Nutrition Quotient for Elderly (NQ-E) for the Elderly in Changwon City (창원시 노인의 영양지수에 영향을 미치는 요인)

  • Seo, Eun Hee
    • The Korean Journal of Food And Nutrition
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    • v.35 no.2
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    • pp.75-87
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    • 2022
  • The purpose of this study was to identify nutrition quotient for elderly (NQ-E) and to investigate factors affecting NQ-E of the elderly using welfare center and senior citizen's center in Changwon city. A self-administered questionnaire was conducted between June 2021 and early August 2021 for 320 elderly (≥65 years, male, n=52, female, n=268). As a result of the survey, the mean NQ-E score was 61.12, which was within the medium-high grade. The scores of balance, moderation, and dietary behavior factors were within the medium-high grade, while diversity factor was within the medium-low grade. According to the results of the Pearson's correlation coefficient analysis, NQ-E showed positive correlations with the 'with family' (p<0.01), 'education level' (p<0.01), 'health functional foods consumption' (p<0.01), 'monthly household income' (p<0.05), 'nutrition education experience' (p<0.05), 'reading nutrition labeling of health functional foods' (p<0.01), 'perception of the efficacy of health functional foods' (p<0.01), and 'the number of times of leisure activities per week' (p<0.01) and negative correlations with the 'gender' (p<0.05), 'age' (p<0.01), 'smoking' (p<0.05), and 'social frailty' (p<0.01). As a result of multiple regression analysis, 'gender' (p<0.05), 'perception of the efficacy of health functional foods' (p<0.05), 'the number of times of leisure activities per week' (p<0.05), and 'social frailty' (p<0.05) were found to be factors affecting NQ-E. Based on the results of this study, customized services by characteristic, nutrition education, and counseling for the elderly should be implemented and the development of various programs and continuous support of the community are necessary so that the elderly can carry out social exchange.

Quantifying L2ers' phraseological competence and text quality in L2 English writing (L2 영어 학습자들의 연어 사용 능숙도와 텍스트 질 사이의 수치화)

  • Kwon, Junhyeok;Kim, Jaejun;Kim, Yoolae;Park, Myung-Kwan;Song, Sanghoun
    • Annual Conference on Human and Language Technology
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    • 2017.10a
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    • pp.281-284
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    • 2017
  • On the basis of studies that show multi-word combinations, that is the field of phraseology, this study aims to examine relationship between the quality of text and phraseological competence in L2 English writing, following Yves Bestegen et al. (2014). Using two different association scores, t-score and Mutual Information(MI), which are opposite ways of measuring phraseological competence, in terms of scoring frequency and infrequency, bigrams from L2 writers' text scored based on a reference corpus, GloWbE (Corpus of Global Web based English). On a cross-sectional approach, we propose that the quality of the essays and the mean MI score of the bigram extracted from YELC, Yonsei English Learner Corpus, correlated to each other. The negative scores of bigrams are also correlated with the quality of the essays in the way that these bigrams are absent from the reference corpus, that is mostly ungrammatical. It indicates that increase in the proportion of the negative scored bigrams debases the quality of essays. The conclusion shows the quality of the essays scored by MI and t-score on cross-sectional approach, and application to teaching method and assessment for second language writing proficiency.

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Quantifying L2ers' phraseological competence and text quality in L2 English writing (L2 영어 학습자들의 연어 사용 능숙도와 텍스트 질 사이의 수치화)

  • Kwon, Junhyeok;Kim, Jaejun;Kim, Yoolae;Park, Myung-Kwan;Song, Sanghoun
    • 한국어정보학회:학술대회논문집
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    • 2017.10a
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    • pp.281-284
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    • 2017
  • On the basis of studies that show multi-word combinations, that is the field of phraseology, this study aims to examine relationship between the quality of text and phraseological competence in L2 English writing, following Yves Bestegen et al. (2014). Using two different association scores, t-score and Mutual Information(MI), which are opposite ways of measuring phraseological competence, in terms of scoring frequency and infrequency, bigrams from L2 writers' text scored based on a reference corpus, GloWbE (Corpus of Global Web based English). On a cross-sectional approach, we propose that the quality of the essays and the mean MI score of the bigram extracted from YELC, Yonsei English Learner Corpus, correlated to each other. The negative scores of bigrams are also correlated with the quality of the essays in the way that these bigrams are absent from the reference corpus, that is mostly ungrammatical. It indicates that increase in the proportion of the negative scored bigrams debases the quality of essays. The conclusion shows the quality of the essays scored by MI and t-score on cross-sectional approach, and application to teaching method and assessment for second language writing proficiency.

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Relationship between Personality Type, SAT score and GPA of Student Nurses (일 대학교 간호대학생의 성격유형과 입학성적 및 학업성취도의 관계)

  • 임지영;유일영;오순남
    • Journal of Korean Academy of Nursing
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    • v.31 no.5
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    • pp.835-845
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    • 2001
  • This study was to identify the relationship between personality type, college admission SAT scores and GPA scores of student nurses. Method: The data was collected from 270 student nurses enrolled in a baccaleaureate program in Seoul. MBTI was used to identify students' personality and SAT score and GPA score were collected over 4 years. The collected data was analyzed by using SPSS Win. package. Result: 1. There were slightly more extrovert (E) type (54.4%) students than the introvert (I) type; more sensing (S) type (71.1%) than the intuitive (N) type. 2. The introvert type students had significantly higher SAT scores than those of the extrovert type (p=.002). 3. The judging type students had significantly higher GPA scores throughout their college years than the perceiving type. 4. There was no statistically significant relationship between SAT and GPA scores. SAT scores did not accurately predict students' academic achievement in college in this sample. Conclusion: The distribution of the personality types in the sample was different from the general population which may suggest that college admission criteria is biased toward certain personality type. Since different personality types process information and cope with the outside world differently, effective teaching strategies need to be considered for each class.

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