• Childhood Kidney Diseases
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• v.5 no.2
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• pp.87-99
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• 2001

Purpose: We studied to find out apo-E genotype polymorphism in minimal change nephrotic syndrome(MCNS) and IgA nephropathy(IgAN) and to determine the relationship between apo-E genotype and clinical course of MCNS. Materials and Method: 43 MCNS patients and 15 IgAN patients were examined for apo-E polymorphism. 50 healthy blood donors were examined for apo-E genotype as control. Genomic DNA was prepared front peripheral blood leukocytes according to standard procedures. Results: As compared with control group, e4 allele frequency was significantly increased in MCNS (p<0.01). However, in IgAN e2 allele frequency, however, was 2.6 times higher than normal control (P<0.01). The frequency of e4 allele of frequent relapser group was 4.6 times higher than normal control and was 2 times higher than infrequent relapser group. Conclusion: We think that apo-E typing might be one of the parameters, which should be considered to predict the course of MCNS in children. MCNS with risky HLA profile and E4/4 genotype could indicate the need for a longer steroid administration. And apo-E genotype needs to be considered for the evaluation of therapeutic responses to other drugs. (J, Korean Soc Pediatr Nephrol 2001 ; 5 : 87-99)

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.420-424
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• 1997

By human intestinal bacteria, saikosaponin c was transformed to four metabolites, prosaikogenin E1 (E1) prosaikogenin E2 (E2), prosaikogenin E3 (E3) and saikogenin E. Metabolic time course of saikosaponin c was as follows; in early time, saikosaponin c was converted to E1 and E2, and then these were transformed to saikogenin E via E3. Also, this metabolic pathway was similar to the metabolism of saikosaponin c by rat intestinal bacteria. Bacteroides JY-6 and Bacteroides YK-4, the bacteria isolated from human intestinal bacteria, could transform saikosaponin c to E via E1 (or E2) and E3. However, these bacteria were not able to directly transform El and E2 to saikogenin E. Naringin was mainly transformed to naringenin by human intestinal bacteria. The minor metabolic pathway transformed naringin to naringenin via prunin. By JY-6 or YK-4, naringin was metabolized to naringenin only via prunin.

• Korean journal of applied entomology
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• v.44 no.2
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• pp.131-137
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• 2005

Seasonal occurrence of the egg parasitoid of bugs, Ooencyrtus nezarae (Hymenoptera: Encyrtidae), was monitored at soybean fields and university campus using traps baited with aggregation pheromone of bean bug, Riptortus clavatus, in Jinju, Gyeongnam province, Korea. The female O. nezarae captured at university campus in 2003 and 2004 was 317.5 and 103.4 times as many as males, respectively, and it was 12.6 times at soybean field in 2004. The female began to occur from late August, showing its peak around September 20 at soybean field in 2004. At university campus in 2003 and 2004, the female began to occur from mid or late August, and showed its peak early or mid September. At the two monitoring sites the catches of female sharply declined after October The E2HZ3H only showed attractiveness to O. nezarae, among three components of the aggregation pheromone of bean bug, (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), (E)-2-hexenyl (E)-2-hexenoate (E2HE2H), and myristyl isobutyrate (MI). A mixture in a ratio of 20:20:10 or 16.7:16.7:16.7 of E2HZ3H:E2HE2H:MI attracted significantly more females than the mixture of 7:36:7 ratio did. This higher attractiveness of the former two blends may be attributed to the higher amounts of E2HZ3H in the blends.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.246-251
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• 1999

This study was designed to evaluate the mutagenicity and the primary mutagenic mechanism of chloropropanols by using various genotypes of E. coli WP2, E. coli TK and E. coli GW series strains. Chloropropanols showed the low mutagenic activities in E. coli WP2s and WP2 establishing the following order; 2,3-DCP> 3-MCPD>1,3-DCP. As compared with E. coli WP2s, the decrease of mutagenic activity and the increase of survival rate in E. coli WP2 $(WP2s\;uvrA^+)$ suggest that DNA lesions produced by chloropropanols could be easily removed by excision-repair system. From the diminution of mutagenic activity and survival rate in E. coli CM611 (WP2s lexA), it was confirmed that the mutagenesis by chloropropanols was dependent on the SOS-repair system. This fact could be also confirmed from the result that both the mutagenic activity and survival rate in E. coli TK610 (umuC) were much lower than those in E. coli TK603 $(umuC^+)$. In the experiment to examine the possibility that chloropropanols might have effects on the LexA of SOS response negative regulator, there was no variation in ${\beta}-galactosidase$ activities of E. coli GW1105 $[lexA3\;(Ind^-)]$ and GW1107 [lexA51 (Def)] by addition of the compounds, indicating that chloropropanols do not have any effects on the LexA, itself.

• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1535-1538
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• 2009

We present calculations for $S_N$2/E2 reactions in protic solvents (t-butyl alcohol, ethylene glycol). We focus on the role of the hydroxyl (-OH) groups in determining the $S_N$2/E2 rate constants. We predict that the ion pair E2 mechanism is more favorable than the naked ion E2 reaction in ethylene glycol. E2 barriers are calculated to be much larger (~ 9 kcal/mol) than $S_N$2 reaction barriers in protic solvents, in agreement with the experimental observation [Kim, D. W. et al. J. Am. Chem. Soc. 2006, 128, 16394] of no E2 products in the reaction of CsF in t-butyl alcohol.

• Clinical and Experimental Pediatrics
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• v.51 no.1
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• pp.42-46
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• 2008

Purpose : Apolipoprotein E (Apo E) plays a major role in lipoprotein metabolism and lipid transport. Many investigators have described that Apo E polymorphisms is one of the most important genetic determinants for cardiovascular disease. The purpose of this study was to evaluate the association between Apo E polymorphisms and serum lipid profiles in obese adolescent. Methods : We measured the serum concentrations of glucose, apolipoprotein (Apo) A1, Apo B, total cholesterol (TC), triglyceride (TG), HDL and LDL-cholesterol after overnight fasting in obese adolescent. Apo E polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results : 86 obese adolescents participated in this study. The body mass index (BMI) of participants were excess of 95 percentile by age and sex. Male to female ratio was 1.7 and mean age of study group was $16.2{\pm}1.8\;years$. Mean BMI was $27.4{\pm}2.5kg/m^2$. The frequency of ${\varepsilon}2$, ${\varepsilon}3$ and ${\varepsilon}4$ allele were 8.1%, 87.2% and 4.7% respectively. Study populations were classified into the following three genotypes 1) Apo E2 group (n=13, 15.1%) carrying either the ${\varepsilon}2/{\varepsilon}2$ or ${\varepsilon}2/{\varepsilon}3$ 2) Apo E3 group (n=65, 75.6%) carrying the most frequent ${\varepsilon}3/{\varepsilon}3$ 3) Apo E4 group (n=8, 9.3%) carrying either the ${\varepsilon}3/{\varepsilon}4$ or ${\varepsilon}4/{\varepsilon}4$. No differences were found among Apo E genotypes concerning age, sex, weight, height and BMI. Apo B and LDL-cholesterol concentrations were significantly higher in the Apo E4 group (P<0.05). No association were found between Apo E genotypes and glucose, Apo A1, TC, TG and HDL. Conclusions : We confirmed that serum concentrations Apo B and LDL-cholesterol were influenced by Apo E genotypes. Apo E polymorphisms seems to influence some alteration of lipid metabolism associated with obesity in adolescent.

• YAKHAK HOEJI
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• v.48 no.4
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• pp.254-260
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• 2004

Structure-activity relationship studies of allylamine type of antimycotics were carried out to evaluate the effect of naphthyl and methyl portion of naftifine. Compounds with 2,4-difluorophenyl( 2a-5a), 2,5-difluorophenyl(2b-5b), 4-ethylphenyl(2c-5c), 2-hydroxyphenyl(2d-5d) and 2-methylnaphthyl(2e-5e) instead of naphthyl group with hydrogen(3a-3e), methyl(4a-4e), and ethyl(5a-5e) in the place of methyl in naftifine were synthesized and tested their in vitro anti-fungal activity against five different fungi. Eight compounds( 3a, 4a, 5a, 3d, 4d, 4d, 5d, 3e, and 4e) showed significant anti-fungal activity against T. mentagroPhytes. (E)-N-(3-Phenyl-2-propenyl)-2- hydroxy-benzenemethaneamine( 3d) displayed moderate antifungal activity against all five different fungi.

• Journal of Life Science
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• v.8 no.2
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• pp.189-196
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• 1998

A family of glycosylphosphatidylinositol-linked ADP-ribosyltransferases, of which cDNAs were cloned from various animal cells, possess a common Glu-rich motif (EEEVLIP) near their carboxyl termini. A similar notif was observed in the sequence of the mouse lymphocyte ADP-ribosyltransferase (Yac-s). Yac-2 has significant NAD glycohydrolase activity as well as ADP-ribosyltransferase activity. To verify the role of the Glu-rich motif in Yac-2, site-directed mutagenesis was performed. Mutants E220Q, E220A, E222A were inactive for ADP-ribosyltransferase activity. For NAD glycohydrolase activity, E220A, E222Q, and E222A were inactive. In contrast, E220Q was active as wild-type. Thus, Glu-220 and Glu-222 in Yac-2 are critical for ADP-ribosyltransferase and NAD glycohydrolase activity, indicating that the Glu-rich motif near the carboxy terminus plays an important role in the Yac-2 enzyme activity.

• Korean Management Science Review
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• v.28 no.1
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• pp.75-90
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• 2011

In B2B e-Commerce market, many companies used in B2B e-Commerce Guarantee Service. Guarantee service raised the stability of collect debts for seller, and made purchase fund for buyer. In 2001, The first year of implementation, B2B e-Commerce Guarantee Cost was just a 19 billion, but in 2008 was significantly increased a 3 trillion 982 billion. In previous studies, however, there was no research on performance management. This study focus on design of KPI weighting for measuring performance of B2B e-Commerce Guarantee Intermediary Service. Through analysis for 'B2B e-Commerce Guarantee Intermediary Service', the study selected KPI and made the KPI weighing. the process of designing a KPI weighting is 5 phase. This study suggests the standard of measuring performance and guidelines on the focus management of KPI in B2B e-Commerce Guarantee Service.

• Journal of Sasang Constitutional Medicine
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• v.9 no.2
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• pp.245-261
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• 1997

The investigation were carried out the 37 patient with cerebrovascular accidents who had been treated in Oriental Medical Hospital in Kyung Hee University and in KangNam Uy Lim, and the 10 examinees who had been worked in Medical Hospital in Kyung Hee University. The consitution which had discriminated by survey, which had discriminated by study on the morphological diagrammings, which had discriminated by the result of the impatient of hospital on a dosage of constitutial prescription. The Prostaglandin E2 in blood for 47 persons (37 patients & 10 examinees) was measured. The following results were obtained. 1.In the constitution which had discriminated by survey, in the value of Prostaglandin E2 in bood of ammong four constitutiens didn,t showed significant differance. 2.In the constitution which had discriminated by study on the morphological diagrammings, in the value of Prostaglandin E2 in bood of ammong four constitutiens didn,t showed significant differance. 3.In the constitution which had discriminated by the result of patient of hospital on a dosage of constitutial prescription, the value of Prostaglandin E2 in blood of among four constitutions didn,nt showed signicant differance. 4.In the constitution which had discriminated by colligated three methods(survey, morphological diagrammings,the result of patient of hospital on a dosage of constitutial prescription), the value of Prostaglandin E2 in blood of ammong four constituion didn,t showed significant differnce. 5.In the constitution which had discriminated by survey, a test of significance between the value of Prostaglandin E2 in blood of each constitution and the value of Prostaglandin E2 in blood of the others was conducted. Only the value of Prostaglandin E2 in blood between in the TAE-EUM-IN(太陰人) and the others of female showed significant differance. 6.In the constitution which had discriminated by study on the morphological diagrammings,a test of significance between the value of Prostaglandin E2 in blood of each constitution and the value of Prostaglandin E2 in blood of the others was conducted. Only the value of Prostaglandin E2 in blood between in the SO-EUM-IN(少陰人) and the others of male showed significant differance. 7.In the constitution whichhad discriminated by the result of patient of hospital on a dosage of constitutial prescription,a test of significance between the value of Prostaglandin E2 in blood of each constitution and the value of Prostaglandin E2 in blood of the others was conducted. Only the value of Prostaglandin E2 in blood between in the TAE-EUM-IN(太陰人) and the others of male showed significant differance. 8.In the constitution which had discriminated was the colligated three methods(survey,morphological diagrammings,the result of patient of hospital on a dosage of constitutial prescription),a test of significance between the value of Prostaglandin E2 in blood of each constitution and the value of Prostaglandin E2 in blood of the others didn,t showed significant differnce. In this connected study is inadequete at present. But if the study is done persistently,the analysis of various ingredient in the body enables objectification in differentiation of four types of physical constitution as the supplemental method.