• Korean Journal of Ecology and Environment
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• v.36 no.4 s.105
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• pp.403-412
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• 2003

Temporal and spatial variations of size-structured phytoplankton (chlorophyll a) were investigated over an annual cycle (February-October, 2003) to elucidate phytoplankton dynamics in the Juam Reservoir, Chonnam. Physical properties were also measured to investigate the relationship between the properties and temporal and spatial variations of size structured phytoplankton using simple linear regression. Phytoplankton (chlorophyll a) were grouped into three size classes: micro-size(> 20 ${\mu}m$), nano-size (3-20 ${\mu}m$) and pico-size (< 3 ${\mu}m$) in this study. Physical properties included water temperature, light attenuation coefficients, PAR (photosynthetically active radiation) and turbidity. Maximum chlorophyll a was observed in April, 2003 in the lower region whereas a peak of chlorophyll a developed in October, 2003 in the upper region. Large cell-sized phytoplankton (micro-size class)were dominant in the events of the chlorophyll a peaks. Potential mechanisms in the physical properties affecting the size-structured phytoplankton dynamics in the Juam Reservoir were discussed.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.659-664
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• 2013

The aim of this study is to determine the drought stress index through photochemical analysis in red pepper (Capsiumannuum L.). The photochemical interpretation was performed in the basis of the relation between Kautsky effect and Photosystem II (PSII) following the measurement of chlorophyll, pheophytin contents, and $CO_2$ assimilation in drought stressed 5-week-old red pepper plants. The $CO_2$ assimilation rate was severely lowered with almost 77% reduction of chlorophyll and pheophytin contents at four days after non-irrigation. It was clearly observed that the chlorophyll fluorescence intensity rose from a minimum level (the O level), in less than one second, to a maximum level (the P-level) via two intermediate steps labeled J and I (OJIP process). Drought factor index (DFI) was also calculated using measured OJIP parameters. The DFI was -0.22, meaning not only the initial inhibition of PSII but also sequential inhibition of PSI. In real, most of all photochemical parameters such as quantum yield of the electron transport flux from Quinone A ($Q_A$) to Quinone B ($Q_B$), quantum yield of the electron transport flux until the PSI electron acceptors, quantum yield of the electron transport flux until the PSI electron acceptors, average absorbed photon flux per PSII reaction center, and electron transport flux until PSI acceptors per cross section were profoundly reduced except number of QA reducing reaction centers (RCs) per PSII antenna chlorophyll (RC/ABS). It was illuminated that at least 6 parameters related with quantum yield/efficiency and specific energy fluxes (per active PSII RC) could be applied to be used as the drought stress index. Furthermore, in the combination of parameters, driving forces (DF) for photochemical activity could be deduced from the performance index (PI) for energy conservation from photons absorbed by PSII antenna until the reduction of PSI acceptors. In conclusion, photochemical responses and their related parameters can be used as physiological DFI.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.479-483
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• 2003

Knowledge of N accumulation during the vegetative growth stage of rice (Oryza sativa L.) is useful for determining the need for topdressing fertilizer N at panicle initiation and booting stage. The chlorophyll content showing SPAD threshold values generated by a simple and portable diagnostic instrument, chlorophyll meter, enables implement the measures of greenness and/or relative chlorophyll contents of leaves. Two-year field experiment was conducted to evaluate the possible application of the chlorophyll meter for N diagnosis in transplanted paddy rice. The chlorophyll meter threshold values (SPAD reading) were taken from uppermost fully expanded leaves at 10days interval from 10 days after transplanting. SPAD readings and N contents of rice leaf blade were closely related at the maximum tillering stage. SPAD readings during growth period showed M-shaped distribution, sharply finished to drop at 20 days after heading date. As N fertilizer increased, the SPAD readings increased. The SPAD readings showed more critical and clear difference in immature paddy field than in ordinary paddy field, where the former soil showed higher response to N fertilizer topdressed. Based on the PAD readings for obtaining an optimum sink size bearing the maximum grain yield, N fertilizer to be dressed at the panicle initiation stage is to be adjusted within the SPA$\times$tillers value range 888 in ordinary paddy, and 800 in immature paddy vice. Among the cultivars tested, Huckhyangbyeo, Jinpumbyeo, Ansungbyeo, Sobibyeo, Manpungbyeo, Sangmibyeo, Jinbongbyeo, showed high SPAD values, whereas the cultivars, Nonghobyeo, Saechuchungbyeo, Hwabongbyeo, Mananbyeo, did low values and others intermediate SPAD threshhold values.

• Korean Journal of Environmental Biology
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• v.31 no.2
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• pp.113-120
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• 2013

This study aimed to investigate the effects of ultrasonic wave on colonial changes of Microcystis sp. causing water-bloom. The effects of frequency change and exposure time of ultrasonic wave on Microcystis sp. were also perceived by measuring the concentration of chlorophyll-a. The concentrations of chlorophyll-a showed a decreasing tendency in all experimental groups exposed to ultrasonic waves, especially the highest decrease at 70 kHz. Before exposed to ultrasonic wave, single cells of Microcystis sp. were observed in a form of colonies which were densely accumulated onto their mucilage layer. However, after one hour exposure to 70 kHz ultrasonic wave, the bonds of colonies were weakened and single cells were separated from the colonies. Moreover, the single cells of the colony were destroyed with the 5 hour exposure time at 70 kHz. As a result, we proposed that the frequency strength and the exposure time of ultrasonic wave might have effects in decrease of biomass of blue-green algae by reducing the concentration of chlorophyll-a, thereby changing and destroying forms of Microcystis sp. colonies.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.23 no.4
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• pp.393-399
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• 2005

Since vegetations are near the wavelength range in 700nm and have absorbent as well as reflective wavelength ranges, there is a much difference in terms of its reflection rate. There are currently many researches on vegetation index being conducted in order to apply the remote-sensing technology to vegetations rising their characteristics of absorbent and reflective wavelength ranges. Normalized Difference Vegetation Index (NDVI) and Perpendicular Vegetation Index (PVI) have been most commonly used. It is usually the evaporation, carbon-dioxide consumption, and chlorophyll density that represent the activity of vegetation, but chlorophyll density is the most commonly used among them. Since the red wavelength range used to obtain the NDVI and PVI has a strong extinction of chlorophyll, it is also useful to test chlorophyll density. The NDVI, in particular, is used to identify the vegetation conditions summarily, and thus, is suitable for initiative researches. Nevertheless, since these vegetation index produce mixed information of the Vegetation vigor and vegetation cover, it is essential to monitor a wavelength range that is independent from redundancy of the Vegetation vigor and vegetation cover. Although many vegetation indices have evaluated both the vegetation vigor and Vegetation cover simultaneously, this research intends to emphasize the utility of separable evaluations of the Vegetation vigor and Vegetation Cover rate through an experiment with grasses. As a result of evaluating vegetation index using spectral reflectance, a separable evaluation of the vegetation vigor and cover has been found more useful.

• Journal of Biosystems Engineering
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• v.24 no.6
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• pp.539-546
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• 1999

Some stress for a plant could be detected to a certain degree by plant physiological measuring technique of the state of the art. The capability of early detection of my measuring system depends on kind of plant and kind and level of stress. The objectives of this study were to evaluate the capability of several fast and intact type plant stress detection systems to detect nitrogen deficiency of cucumber in the field. A series of experiment was carried out with four kinds of intact type measuring devices - a chlorophyll content meter, a chlorophyll fluorescence measurement system, an infrared thermometer and an optical spectrometer. The experiments resulted that the chlorophyll content meter could detect the stress of N deficiency at a confidence level higher than 95% on 3rd day for the earliest case and the detection of high precision was possible from 7th day after the stress was applied. The chlorophyll fluorescence measurement system detected the stress at a confidence level higher than 95% on 3rd day for the earliest case but the detection was not as much precise as the chlorophyll content meter. Leaf temperature measurement noted very poor results to detect the stress. Using the spectrometer, sensitive wavelength regions to detect the stress were searched and found out as 562∼564 nm, 700∼724 nm and 1,886∼1,894 nm. With the spectrometer using any of wavelength within the sensitive wavelength region, detection of the stress at a confidence level higher than 95% was possible from 3rd or 4th day after the stress was applied.

• Korean Journal of Weed Science
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• v.11 no.1
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• pp.68-73
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• 1991

This study was conducted to determine the appropriate culture conditions for developing photoautotrophic cells from tobacco using plant growth regulators, sucrose and light condition in LS medium. The greatest callus induction was observed in the LS medium supplemented with $10^{-5}$ M NAA and $10^{-4}$ M BA, showing 3.08 g of callus fresh weight determined at 30 days after incubation. The highest chlorophyll content of callus was obtained in the LS medium supplemented with $10^{-5}$M NAA and $10^{-6}$ M BA, showing 28.42${\mu}g/g$. In both light and dark conditions, callus induction increased as the concentration of sucrose increased from 0.5%to 3.0%. In particular, in the light condition, the greatest callus induction was made in the LS medium supplemented with 2% sucrose. On the other hand, the heighest chlorophyll content was observed at 0.5% sucrose and chlorophyll wasn't induced in dark condition. The chlorophyll content of callus cultured in LS medium containing 0.75% sucrose was similar to 1% sucrose, as far as the chlorophyll content of callus was concerned.

• Korean Journal of Weed Science
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• v.8 no.2
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• pp.176-181
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• 1988

This experiment was conducted to investigate the effect of plant hormones on the time-dependent changes of photosynthesis by oxygen electrode system (Rank Brothers Inc. England) and chlorophyll contents in rice(Oryza sativa L. cv. Nihonbare) leaf discs. IAA showed no significant effect when treateds at lower concentration, however IAA 20ppm inhibited slightly the photosynthesis of leaf discs. BA increased strongly the photosynthesis with time. As the BA concentration increased, photosynthesis was significantly stimulated. $GA_3$ showed with little effect at lower concentration, however $GA_3$ 20ppm inhibited the photosynthesis. ABA inhibited strongly the photosynthesis at all the treatments. IAA, BA, and $GA_3$ stimulated chlorophyll contents, but ABA inhibited the chlorophyll contents. It can be concluded that among the plant hormones tested, BA was the most effective in stimulating photosynthesis with time and maintaining the chlorophyll contents.

• Korean Journal of Weed Science
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• v.3 no.1
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• pp.100-104
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• 1983

Responses of barley cultivars including 8 covered barley cultivars and 8 naked barley cultivars to butachlor, terbutryn, and methabenzthiazuron were evaluated by determinating crop injury, dry weight and chlorophyll content of barley seedlings. Most barley cultivars were tolerant to butachlor at 1478 ai/10a (recommended rate) but Olbori, Dongbori, and Gangbori were slightly sensitive to butachlor at 294g ai/10a. Sensitivity to terbutryn was generally greater in naked barley cultivars than hulled barley cultivars. Dongbori, Buhobori, and Bunong of hulled barley and Bangsa #6, Kwangseung, Nonsangwa # 1-6, and Iri #4 of naked barley were highly sensitive to terbutryn at 350g ai/10a (double dosage). Most barley cultivars were relatively tolerant to methabenzthiazuron except Iri #4. Dry weight of barley seedlings was not reduced by butachlor, terbutryn, and methabenzthiazuron at recommended rate. However, terbutryn at 350g ai/l0a greatly reduced dry weight of all barley cultivars, especially naked barley cultivars. Chlorophyll content of Owealbori, Dongbori #1, Jogangbori, Bangsa #6, Backdong, and Kwangseung was slightly reduced by butachlor. Terbutryn at 175g ai/10a reduced chlorophyll content of Owealbori, Olbori, and Nonsangwa #1-6 and all cultivars were greatly reduced by terbutryn at 350g ai/10a. Methabenzthiazuron slightly reduced chlorophyll content of Jogangbori, Dongbori #1, Owealbori, Backdong, Kwangseung, and Nonsangwa # 1-6.

• Ocean and Polar Research
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• v.27 no.3
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• pp.251-263
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• 2005

In order to study changes in the marine ecosystem of the East China Sea derived by the global warming and construction of the Three Gorges Dam in the middle of the Changjiang, temperature, salinity, nutrients, and chlorophyll-a were studied intensively in the northern part of the East China Sea during the summer of 2003 and spring of 2004. According to the previous studies, the upwelling of the Kuroshio Current and the Changjiang resulted in a major inputs of nutrients in the East China Sea, but these two inputs may not contribute gently to a build up of nutrients in the northern East China Sea. In spring, relatively high concentrations of nitrates and phosphates were observed in the western part of the study area, which resulted from the supply of high concentrations of nutrients showing up in the surface waters as a result of vertical mixing from the ocean bottom. The concentrations of nitrates and phosphates observed in summer were lower than those in spring, since the surface waters were well stratified by the larger discharge of fresh water from the Changjiang in summer. The surface nitrate/phosphate ratios ranged from 1.3 to 16 in spring and from 1.1 to 15 in summer and were lower than the Redfield ratio of 16, indicating that the growth of phytoplankton is limited by nitrogen. This results are contrary to the previous results, in which the growth of phytoplankton was limited by phosphate in the East China Sea. The reason for this contrary result is that most nutrients in the surface waters are supplied by vertical mixing from the bottom waters with low nitrate/phosphate ratios, not directly influenced by the Changjiang with high nitrate/phosphate ratios. The depth-integrated chlorophyll observed in summer was similar to the previous results, but those measured in spring were almost twice as high as those found in previous results. The depth-integrated chlorophyll in spring was higher than that of summer, which results from high concentrations of nitrates and phosphates in the surface waters in spring due to active vertical mixing.