• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.474-481
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• 1996

Since the commercially available rabbit anti-cyclin D3, generated from c-terminal 16 amino acid residues which are common to human and murine cyclin D3, is highly cross-reactive with many other cellular proteins of mouse, a new rabbit polyclonal anti-cyclin D3 has been raised by using murine cyclin D3 protein expressed at a high level in Escherichia coli as the immunogen. To express murine cyclin D3 protein in E. coli, the cyclin D3 cDNA fragment encoding c-terminal 236 amino acid residues obtained by polymerase chain reaction (PCR) was inserted into the NcoI/BamHI site of protein expression vector, pET 3d. Molecular mass of the cyclin D3 overexpressed in the presence of IPTG (Isopropyl $\beta$-D-thiogalactopyranoside) was approximately 26 kDa as calculated from the reading frame on the DNA sequence, and the protein was insoluble and mainly localized in the inclusion bodies that could be easily purified from the other cellular soluble proteins. When renaturation was performed following denaturation of the insoluble cyclin D3 protein in the inclusion bodies using guanidine hydrochloride, 4.4 mg of soluble form of cyclin D3 protein was produced from the transformant cultured in 100ml of LB media under the optimum conditions. Four-hundred micrograms of the soluble form of cyclin D3 protein was used for each immunization of a rabbit. When the antiserum obtained 2 weeks after tertiary immunization was applied to Western blot analysis, it was able to detect 33 kDa cyclin D3 protein in both murine lymphoma cell line BW5147.G.1.4 and human Jurkat T cells at 3,000-fold dilution with higher specificity to murine cyclin D3, demonstrating that the new rabbit polyclonal anti-murine cyclin D3 generated against c-terminal 236 amino acid residues more specifically recognizes murine cyclin D3 protein than does the commercially available rabbit polyclonal antibody raised against c-terminal 16 amino acids residues.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.113-116
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• 2008

The active layer thickness and curing condition dependent performance of an organic thin film transistor (OTFT) with inkjetted organic semiconductor (OSC) layer is studied The best performance of the OTFT was found when the thickness of ose was ~120 nm cured at $60^{\circ}C$. The performance enhancement of the OTFT with inkjetted OSC layer was discussed by comparing the OTFT with spin-coated ose layer.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 추계학술대회 논문집 학회본부
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• pp.123-126
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• 1996

In this paper, optimal modulation controller is designed to improve the stability of A.C. and A.C.-D.C. power system, and optimal theory is applied to select optimal modulation controller input signal Optimal modulation controller for speed governor and exciter controller system is constructed in A.C. power system, while the controller is constructed to the both control systems like AC. power system, considering ACR-AVR, APR-$A{\gamma}R$ as the control method of direct current system. It is considered that the stability of A.C. power system only and A.C.-D.C. power system against load fluctuations and disturbances under case of optimal modulation control.

• 한국지반공학회논문집
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• 제23권7호
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• pp.87-98
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• 2007

본 연구에서는 DMT를 이용한 비배수 전단강도를 파악하기 위하여 부산신항 점토층에 DMT 시험, 베인전단시험, $CK_0U$ 삼축압축시험을 실시하였다. 시험결과 정규화한 비배수 전단강도비는 삼축시험의 경우 $S_{u(CKU)}/{\sigma}'_v=0.30{\sim}0.35$, 베인전단시험의 경우 ${\mu}S_{u(VST)}/{\sigma}'_v=0.20{\sim}0.22$로 나타났다. 본 연구에서는 현장 베인전단시험 및 삼축압축시험 결과와의 비교를 통해, DMT를 이용하여 비배수 전단강도를 추정하는 두 가지 방법을 제안하였다. 첫 번째는 $S_u/{\sigma}'_v$와 횡방향 응력지수의 관계를 이용하는 방법이고, 두 번째는 $N_c-I_D$ 또는 $N_c-E_D$ 관계로부터 비배수 전단강도를 추정하는 방법이다. 베인전단시험 및 삼축시험 결과와 두 가지 추정방법을 비교한 결과, $N_c-I_D$ 또는 $N_c-E_D$ 관계를 이용한 방법이 $K_D$ 이용하는 방법보다 비배수 전단강도를 정확하게 추정하는 것으로 나타났다. 이것은 $I_D$와 $E_D$가 토질상태를 반영하는 $p_1-p_0$항을 포함하고 있어, $N_c$를 이용하는 방법이 토질을 고려한 비배수 전단강도 추정을 할 수 있기 때문으로 판단된다.

• Journal of Plant Biology
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• 제12권2호
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• pp.7-14
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• 1969

Dunaliella tertiolecta did not show any increase in respiration rate when supplied with glucose, glycerol, sucrose, L-alanine, acetate, pyruvate and succinate. This was in contrast to Chlorella pyrenoidosa, which, under identical conditions, showed significant increase when supplied with glucose or acetate but not with the other compounds. Production of 14CO2 from added 14C-glucose in D. tertiolecta was lower than the other 14C-labelled substrates: L-alinine, glycerol, succinate, but higher than 14C-sucrose addition. And it was also lower than C. pyrenoidosa experiments which was added 14C-glucose as a substrate. Light reduced amounts of labelled carbon dioxide from 14C-glucose or 14C-acetate and increased incorporation of 14C from the substrates to cell materials in either D. tertiolecta or C. pyrenoidosa. The contribution of 14C from 14C-glucose to 14CO2 in cell-free system of D. tertiolecta were much higher than in whole cell suspension. It was contrast to C. pyrenoidosa which were showed reduction of 14CO2 production in cell-free systems than whole cell suspensions. When cell-free systems of D. tertiolecta and C. pyrenoidosa were supplied with ATP, NAD, NADP or/and hexokinase, it was remarkably increased production of 14CO2 from the substrates than the control. It was concluded that the low ability of D. tertiolecta to metabolize glucose were caused by the impermeability of the cell membrane to glucose and were not due to deficiencies of enzyme systems concerning glucose metabolism. In the cell-free systems, it seemed to be more active pentose phosphate pathway than glycolytic pathway in D. tertiolecta.

• 복식문화연구
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• 제28권3호
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• pp.299-312
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• 2020

The purpose of this study is to create a shirt sloper suitable for an elderly male body shape by producing virtual models using a 3D-virtualization program, making a torso prototype using the Yuka CAD system, and employing 3D simulation to virtualize and calibrate the model. First, the following three types of obese dummies are implemented through the CLO 3D program: Type 1 exhibits body fat in the lower body; Type 2 exhibits an obese abdomen; and Type 3 displays a balanced form of obesity. Second, for the design of the shirt pattern, the waist back length (measured value+1), back armhole depth (C/10+12+3+0.5~1.5), front armhole depth (back armhole depth 0~1), front interscye (2C/10-1+0.5-0.5), armscye depth (C/10+2+3.5+ 0.5), back interscye (2C/10-1+1), front chest C (C/4+2.5+1), back chest C (C/4+2.5-1), front hem C (C/4+2.5+1(+2)), back hem C (C/4+2.5-1(+2)), cap height (AH/3-5), and biceps width (Front AH-1, Back AH-1) are calculated. Third, the virtual attachment of the shirt pattern is resolved by increasing the front and back armhole depths, and the front and rear wrinkles are improved by adding a back armhole dart. The front hem lift and lateral pull caused by the protrusion of the abdomen are amended by increasing the margin of the chest, waist C, and hip C, with the appearance improved by balanced margin distribution in the front, back, and side panels. The improved retail pattern with an increase in the front armholes C was balanced on the torso plate.

• 한국CDE학회논문집
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• 제3권2호
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• pp.127-134
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• 1998

This paper presents d new algorithm to compute the offlet curve of a given planar parametric curve. We reduce the problem of computing an offset curve to that of intersecting a surface to a paraboloid. Given an input curve C(t)=(x(t), y(t))∈R², the corresponding surface D/sub c(t)/ is constructed symbolically as the envelope surface of a one-parameter family of tangent planes of the paraboloid Q:z=x²+y²along a lifted curve C(t)=(x(t), y(t), x(t)²+y(t)²∈Q. Given an offset distance d∈R, the offset curve C/sub d/(t) is obtained by the projection of the intersection curve of D/sub c(t)/ and a paraboloid Q:z=x²+y²-d² into the xy-plane.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.226-229
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• 2005

An experiment was conducted to produce eggs enriched with vitamins $D_3$, K and iron in eggs. Six hundred 97-wk-old ISA Brown force molted hens were allocated to completely randomized block arrangement of six dietary treatments: T1; control (C), T2; C+4,000 IU vitamin $D_3$+2.5 mg vitamin K+100 ppm Fe, T3; C+8,000 IU vitamin $D_3$+5.0 mg vitamin K+100 ppm Fe, T4; C+12,000 IU vitamin $D_3$+7.5 mg vitamin K+100 ppm Fe, T5; C+16,000 IU vitamin $D_3$+10.0 mg vitamin K+100 ppm Fe, T6; C+20,000 IU vitamin $D_3$+12.5 mg vitamin K+100 ppm Fe. Fe was supplemented with Fe-methionine. Each treatment consisted of five replicates of ten cages with two birds per cage. Egg production and egg weight were highest in T2 and incidence of soft and broken egg was highest in T6. Haugh unit was not different among treatments although it tended to be increased as dietary vitamins $D_3$ and K increased. Eggshell strength was not different among treatment. Concentrations of vitamin $D_3$ and K in egg yolk increased and plateaued approximately 20 days after feeding supplemented diets. The level of these vitamins peaked at 12,000 IU/kg vitamin $D_3$ and 7.5 mg/kg vitamin K supplementation and then decreased at the higher than these supplementation levels. The peak concentrations of vitamin $D_3$ and vitamin K were 4.6 times and 4.8 times greater than the control, respectively. Supplementary Fe also increased Fe content in egg yolk. It is concluded that vitamin $D_3$ and K in eggs can be effectively enriched by proper supplementation time and level of these vitamins.

• 한국안광학회지
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• 제17권2호
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• pp.165-170
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• 2012

목적: 2D와 3D 영상 시청에서 나타나는 시각반응의 변화를 측정하고 평가하고자 하였다. 방법: 시기능이 정상인만 19세 이상 25세 이하의 대학생 44명을 대상으로 하였다. CA/C비(convergence accommodation/convergence ratio), 폭주로 유도되는 PD(interpupillary distance)변화와 조절반응, 3D 영상 시청 시 자각적 인지거리를 측정하였다. 결과: 폭주와 조절은 2D 영상 시청보다 3D 영상 시청에서 더 컸으며(p<0.05), CA/C비와 조절반응은 양적 상관관계(r = 0.477, p = 0.001)를 보였다. PD가 작을수록 심도지각의 깊이는 큰 것으로 나타났으며, 3D 영상에서 폭주는 인지하고 있는 거리에서의 폭주보다 더 큰 것으로 나타났다. 결론: CA/C비가 클수록, PD가 작을수록 3D 영상 시청에서 시각적 불편함은 더 심할 것으로 예상된다.

• 생명과학회지
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• 제23권10호
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• pp.1216-1222
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• 2013

자연살상세포(NK cells)은 림프구계의 세포로서 외부 침임 병원균을 막고 체내 형질변환세포를 제거하는데 참여하고 있다. 이러한 자연살상세포의 활성은 특정한 항원이 필요 없고 활성화 신호와 억제성 신호의 균형에 의해 조절되고 있다. 자연살상세포의 중요한 활성화 신호 중의 하나는 NKG2D 수용체를 통한 것인데, 이 NKG2D 수용체를 통해 자연살상세포는 암세포에 있는 NKG2D 리간드를 인식할 수 있다. 지금까지 인간에서는 여덟개의 NKG2D 리간드가 밝혀져 있고 이러한 리간드의 발현은 다양한 기전을 엄격하게 조절되고 있다. 암세포는 암유전자(oncogenes)에 의해 세포내 다양한 유전자의 발현이 정상세포와 확연히 달라지는데, 이러한 암유전자에 의해서 NKG2D 리간드의 발현이 영향을 받을 것으로 생각되어 진다. 이 연구는 인간의 암세포에서 가장 자주 발현되는 세가지 암유전자 k-ras와 c-myc, wnt의 억제를 통해 NKG2D 리간드의 발현이 어떻게 변화되는 지를 알아보았다. k-ras와 c-myc의 억제는 NKG2D 리간드의 발현을 효과적을 증가시켰고 암세포가 자연살상세포에 더욱 잘 죽게 변화되었다. 그러나 wnt 억제는 MICA와 ULBP1의 전사를 감소시켰다. wnt 억제에 의한 NKG2D 리간드의 전사억제에도 불구하고 세포막의 단백질 발현은 변하지 않아서 암세포의 자연살상세포에 대한 감수성은 별다른 변화를 보이지 않았다. 따라서 k-ras와 c-myc, wnt 억제는 각각 다른 반응을 보였으며 최종적인 자연살상세포에 대한 감수성은 NKG2D 리간드의 세포표면단백질 발현정도에 의해 결정됨을 알 수 있었다.