• 대한본초학회지
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• 제23권2호
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• pp.151-157
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• 2008

Objectives : The purpose of this study is to investigate the biological Effect of multi-herbal drug 'KOCO-P1' on mouse macrophage Raw 264.7 cells. Methods : Multi-herbal drug 'KOCO-P1' was composed of Ginseng Radix, Astragali Radix, Polygonati Rhizoma, Liriopis Tuber, and Scrophulariae Radix. Cytotoxicity and cytoprotective activity of K0C0-P1 was verificated by MTT assay. And antioxidative effect of K0C0-P1 against EtOH, Nicotine was inspected by Hydroperoxide assay. Results : K0C0-P1 showed no cytotoxicity on RAW 264.7 cells for 24, 48, 72 hours. KOCO-P1 at 200, 100, and 50 ug/mL reduced the production of H202 in Raw 264.7 cells by EtOH. KOCO-P1 at 50 ug/mL reduced the production of H202 in Raw 264.7 cells by Nicotine. Conclusions : KOCO-P1 could be supposed to have antioxidative effect on macrophage with no cytotoxicity.

• Bulletin of the Korean Chemical Society
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• 제19권11호
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• pp.1244-1248
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• 1998

Ozonolysis reactions of cyclic olefins 1a-c and norbomene In in the presence of trifluoroactophenone 6 provided the corresponding cross-ozonides 7a-c and 7n. Further reactions of ozonides 7a-c and 7n with the independently prepared carbonyl oxide 11 gave diozonides of structure l0a-c and 10n. The ozonolysis of 1methylcyclopentene 12a and 1-methylcyclohexene 12b in the presence of trifluoroactophenone 6 provided exclusively ozonide 15 and 16 derived from capture of carbonyl oxide 13. All of the new ozonides have been isolated as pure substances and characterized by their 1H NMR and 13C NMR spectra.

• 한국작물학회지
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• 제59권4호
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• pp.435-444
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• 2014

본 연구는 옥수수수염에 함유된 flavonoid 계열의 물질인 maysin, luteolin 7-O-neohesperidoside, luteolin 3'-methyl ether 7-glucuronosyl-($1{\rightarrow}2$)-glucuronide 및 polyphenol성 물질인 chlorogenic acid을 추출 및 분리하는 방법에 관한 것으로, 얻어진 결과를 요약하면 다음과 같다. 1. 수정되지 않은 옥수수수염의 메이신 함량은 출사 후 3일에 함량이 최대치에 도달하고, 그 후 감소되는 것으로 나타났으며, 방임수분된 옥수수수염의 메이신 함량은 출사후 지속적으로 감소되는 것으로 나타나 화분의 수분 여부에 따라 메이신 함량에 많은 차이가 있음을 알 수 있었다. 2. 채취한 옥수수수염에 에탄올을 가하여 9일간 상온에서 추출 후 엽록소, 지질 및 당질 등을 제거시키고, $C_{18}$ column chromatography를 수행하여 분취물 I, II, III, IV를 얻었다. 3. 분취물의 흡광도를 분석한 결과 분취물 I은 327 nm 및 239 nm에서 최대 흡수 파장(${\lambda}_{max}$)을 나타내었고, 분취물 II는 339 nm 및 274 nm, 분취물 III는 345 nm 및 277 nm, 분취물 IV는 352 nm, 270 nm, 및 257 nm 에서 최대 흡수파장을 나타내었다. 4. 분취물 I은 m/z $355[M+H]^+$인 chlorogenic acid(3-(3,4-dihydroxycinnamoyl)quinic acid, $C_{16}H_{18}O_9$)이었으며, 분취물 II는 chlorogenic acid와 m/z $653[M+H]^+$인 luteolin 3'-methyl ether 7-glucuronosyl-($1{\rightarrow}2$)-glucuronide ($C_{28}H_{28}O_{18}$)를 함유한 혼합물, 분취물 III는 chlorogenic acid와 m/z $595[M+H]^+$인 luteolin 7-O-neohesperidoside ($C_{27}H_{30}O_{15}$) 및 luteolin 3'-methyl ether 7-glucuronosyl-($1{\rightarrow}2$)-glucuronide를 함유한 혼합물이었고, 분취물 IV는 m/z $577[M+H]^+$인 maysin ($C_{27}H_{28}O_{14}$, 2"-O-${\alpha}$-L-rhamnosyl-6-C-(6-deoxy-xylo-hexose-4-ulosyl)luteolin 임을 각각 확인 하였다. 5. 옥수수수염의 알코올 추출물로부터 분리된 분취물 I은 옥수수수염 생체 100 g 당 (수분함량 약 92%) 35 mg/100 g, 분취물 II는 48 mg/100 g, 분취물 III는 46 mg/100 g, 분취물 IV는 138 mg/100 g을 얻을 수 있었다.

• 미생물학회지
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• 제5권1호
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• pp.20-23
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• 1967

I studied about temperature and pH concentration having influence upon chlorophyll decrease on the Glucose culture of Chlorella variegata Beijerinck(211/10a). I cultured under $25^{\circ}C$(standard), $15^{\circ}C$ and $35^{\circ}C$ and compared with each other. The culture of $15^{\circ}C$ didn't have any large difference with the standard culture($25^{\circ}C$) but the culture of $35^{\circ}C$ had a large amount of chlorophyll decrease without carbohydrate accumulation, stimulation of cell division and nitrogen-deficiency. Chlorella variegata had optimum pH 6.5-7 and was a little weak in all phenomenon under pH 8 rather than under pH 6.5-7. Under pH 5 they had deep chlorophyll decrease without phephytin.

• 아시안잔디학회지
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• 제12권4호
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• pp.211-220
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• 1998

Cellulose-degrading fungi were idenfied as Rhizoctonia solani AG2-2(IV), T. harzianum and C. cochliodes. Rhizoctonia solani AG2-2(IV) grows better in the acidified media of pH 4 and 5 than pH 6 and 7. Mycelial growth of T. harzianum and C. cochliodes was also higher in pH 4 and 5 than in pH 6 and 7. In order to relate the above findings to nutrient utilization, mycelial growth of R. solani AG2-2(IV) are evaluated with various carbon sources. R. solani AG2-2(IV) grows well in the order of mannose, cellobiose, glucose, xylose and arabinose. However, mycelial dry weights of T. harzianum were 98.7, 78.0, 72.3, 43.7 and 32.3mg in glucose, mannose, cellobiose, xylose, and arabinose, respectively. Mycelial dry weight of C. cochilodes was 118, 65, 57, 49, and 16mg in mannose, cellobiose, xylose, glucose, and arabinose, respectively. Result of cellulase assay of R. solani AG2-2(IV) and soil fungi was reffered as, R. solani AG2-2(IV) produced more cellulase on CMC substrate than on CEL and secretes more enzyme in floated condition than in water-immersed condition. T. harzianum secreted less amount of cellulase than R. solani AG2-2 and C. cochliodes. T. harzianum produced no enzyme on CEL under water-immersed condition. C. cochliodes produced similar amounts of cellulase on either CMC or CEL under both water-immersed and floated condition.

• 한국응용과학기술학회지
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• 제26권2호
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• pp.191-198
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• 2009

We have tested the stability of paeoniflorin, a new cosmetic ingredient, extracted from the roots of Paeoniae lactiflora. The stability of aqueous paeoniflorin solution at pH 3, 5 and 7 varied by adding buffer solution was tested at $0^{\circ}C,\;25^{\circ}C,\;40^{\circ}C,\;and\;65^{\circ}C$. The test was performed with or without UV light. The solution of paeoniflorin was stable at pH 3.0, however, the recovery rate of paeoniflorin was 40% at pH 7.0. The stability of paeoniflorin solution was decreased as the pH of paeoniflorin solution was increased by pH 7.0. The effect of storage temperature of paeoniflorin solution shows that the stability of paeoniflorin solution was decreased as the temperature was increased. The stability of paeoniflorin was rather good under UV light than the condition given above $40^{\circ}C$. The stability of paeoniflorin in W/O emulsions shows similar pattern to that of aqueous solution.

• 한국자기학회지
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• 제8권5호
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• pp.295-299
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• 1998

페라이트 도금 방법은 진공이 불필요하며 저온(<10$0^{\circ}C$)에서 페라이트 박막을 제작할 수 있다. 이 방법으로 기판온도 8$0^{\circ}C$, 산화용액의 pH 7.1~8.8 영역에서 모두 스피넬 구조의 NixFe3-xO4 (x=0.162~0.138) 박막을 얻었다. 이들은 열처리를 하지 않았음에도 불구하고 결정성이 우수한 다결정의 박막을 얻었다. 박막의 성장속도는 산화용액의 pH가 7.1에서 8.8까지 변함에 따라 성장속도가 143$\AA$/min에서 255 $\AA$/min 까지 증가하였다. 산화용액의 pH가 증가할수록 입경의 크기가 증가하고, 보자력은 감소하였다.

• 한국미생물·생명공학회지
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• 제30권3호
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• pp.258-263
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• 2002

E. faecalis RKYl을 이용한 젖산발효에서 세포성장과 젖산 생산에 대한 배양온도 및 pH의 영향을 조사한 결과온도가 낮을수록 세포성장은 향상되었으나 발효시간이 지연되어 젖산생산이 저하되었으며$ 50^{\circ}C$에서는 세포성장이 거의 없었다. pH 7.0-9.0에서 거의 비슷한 세포성장 및 젖산생산을 보였으나 pH 6.0에서는 세포성장이 저하되었고 발효시간이 지연되었으며, pH 10.0에서는 세포성장이 거의 없었다. E. faecalis RKY에 의한 젖산발효는 비교적 넓은 범위의 배양온도($34- 46^{\circ}C$)및 pH(7.0-9.0)에서 안정하였으며 최적 배양온도 및 pH 는 각각 $42^{\circ}C$, 7.0이었다. 또한 일반적으로 일인산염에 비해 이인산염을 첨가해 주었을 때 젖산생산성이 향상되었으며, 효모추출물 10g·$L^{-1}$을 질소원으로 사용하여 배양하였을때 $Mg^{2+}$ 과 $Mn^{2+}$의 첨가효과는 없는 것으로 나타났다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.374-378
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• 2004

The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and $40^{\circ}C$) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present Study. The highest growth rate is achieved at pH 7, $37^{\circ}C$ and at a rotational Speed of 250 rpm which is 0.927 $h^{-1}$. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a $123\%$ different between the highest growth rate (0.927 $h^{-1}$) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and $40^{\circ}C$. The yield of protein at pH 7 is $154\%$ higher compared to the lowest yield achieved at pH 5. There is about $28\%$ different of the protein yield for the E. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at $40^{\circ}C$ is $38\%$ higher compared to the lowest yield achieved at $30^{\circ}C$.

• 대한화학회지
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• 제40권3호
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• pp.161-166
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• 1996

6-ethyl-5,-6-dihydrouracil($C_6H_10N_2O_2$)은 단사정계인 공간군 $P2_{1c}$으로 a=10.302(2), b=10.419(3), $c=7.095(1)\AA$, $\beta=106.6(0)^{\circ}$, Z=4, $V=729.7(3)\AA^3$, $D_c=1.29g/cm^3,\;{\lambda}(MoK\alpha)=0.71073\AA$, $\mu=0.010$cm^{-1}$, F(000)=304이며, F>4.0 $\sigma(F)$인 1070개의 관측된 회절점에 대한 R값은 0.054였다. 직접법에 의하여 구조를 풀었으며, 전행열-최소자승법에 의하여 정밀화하였고, C-H 결합길이는 $0.96\AA$에 고정하였다. Hydrouracil 고리는 불규칙한 puckered 각을 가진 envelope conformation으로 되어 있고, 고리에 붙어 있는 에틸기는 axial 방향으로 향하고 있으며, 단위격자속에는 100평면에 거의 평행인 2차원의 결합망을 이룬, 두 개의 분자간 수소결합, N(1)-H---O(7)(1+x, 0.5-y, -0.5+z)와 N(3)-H---O(7)(1+x, 0.5-y, 0.5+z)가 있으며, 이웃 분자와의 최단거리는 C(4)---O(8)(-x, -y, 1-z)의 $3.187\AA$이다.