• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.432-438
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• 2013

A study was conducted to examine the effect of maturity and precooling ($60%/0^{\circ}C$ and $80%/4^{\circ}C$), and light illumination on the storage life of 'Maehyang' strawberry meant for export. Fruits at 60% and 80% ripened stage were harvested from a commercial greenhouse in Jinju on April 3, 2012. Harvested fruits were transported to the precooling system within 30 minutes. Transported fruits were precooled the $4^{\circ}C$ for 2 hours and $0^{\circ}C$ for 5 hours by a forced draft cooling system, and then stored at $6^{\circ}C$. During the storage, the fruits were examined for their changes in hardness, soluble solid content, quality grade, acidity, Hunter value, weight loss, and the incidence of gray mold (Botrytis cinerea) at an interval of two days from April 5 to April 17. Hardness was decreased until 7th days and it was changed to increase at 9th days. Treatment of 60% maturity, $0^{\circ}C$ precooling and no light illumination of strawberry were shown the highest value in freshness. The soluble solid content harvested in 80% maturity strawberry was higher than 60% maturity strawberry until the third day. Quality grade decreased rapidly in 80% maturity stage with light illumination strawberry in comparison to the 60% maturity stage of strawberry. Hunter value 'L' and 'a' showed a rapid change in 60% maturity stage of strawberry. Weight loss decreased rapidly in 80% maturity, $0^{\circ}C$ precooling, and no light illumination of strawberry treatments. Gray mold incidence was found the most at 60% maturity, $4^{\circ}C$ precooling, and light illumination of strawberry. The results from our study indicate that effectiveness for keeping the freshness of strawberry was best achieved by harvesting in low maturity, precooling at $0^{\circ}C$, and with no light illumination.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.11
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• pp.1355-1360
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• 2013

A cracked solid propellant would have failure or fracture of rocket because of excessive combustion according to increase of burning area, therefore it is important to evaluate the fracture toughness of solid propellant. A procedure is used to investigate the material under a range of test temperatures between -60 and $60^{\circ}C$, three kind of specimen thickness, 4, 12.5 and 24.5 mm to determine the effect of two parameters on the fracture toughness. A center cracked tension (CCT) specimen is used in these tests, which were conducted using INSTRON 5567 testing machine and environmental chamber to evaluate the fracture toughness. The experimental results show that the fracture toughness tends to decreases with an increase in the temperature, and the effect of thickness indicates that the fracture toughness is highest at 12.5 mm under various temperatures except $-60^{\circ}C$. It is found that the fracture toughness of solid propellant is changed due to glass transition behavior around $-60^{\circ}C$.

• Applied Biological Chemistry
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• v.24 no.3
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• pp.186-193
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• 1981

Three fractions of carboxymethyl-cellulase (F-I, F-II, and F-III) and ${\beta}-glucosidase$ form Aspergillus niger were partially purified by ammonium sulfate fractionation. Sephadex G-150 and DEAE-Sephadex column chromatography. The optimum conditions such as pH and temperature and thermal inactivation properties of the enzymes were investigated. Arrhenius plots of F-II and F-III appeared as straight lines, whereas that of F-I was biphasic. The Z-values of F-II and F-III were $8^{\circ}C$ and $10^{\circ}C$ respectively, while that of F-I was $4^{\circ}C$ over $60{\sim}70^{\circ}C$ and $383^{\circ}C$ over $70{\sim}98^{\circ}C$. Three fractions and the crude extract of carboxymethyl-cellulase exhibited a similar optimum pH 4.3 and temperature of $60^{\circ}C$, while Z-value of crude extract $(21.5^{\circ}C)$ was much higher than that of the purified enzyme. Maximum activity of both purified and crude extract of ${\beta}-glucosidase$ was shown at pH 4.7 and $60^{\circ}C$, and z-value of the enzyme was $7^{\circ}C$.

• Journal of Sericultural and Entomological Science
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• v.28 no.2
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• pp.35-37
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• 1986

To reduce the number of rearing season required for preservation of multivoltine silkworms which do not produce diapause eggs, the optimal egg stage, temperature, and period of cold storage were examinede using hatchability as an indicator of viability. Multivoltine silkworm starains MR, SPT, and HM were used in the study. 1. The hatchability of multivoltine silkworm eggs (MR and STP) preserve at 5$^{\circ}C$ for 30 days was 80% for the eggs chilled from 2 days after oviposition but less 5% for those chilled from 7 days after ovipostion. 2. When 2 day-old eggs of multivoltine silkworm (HM) were preserved between -2.5$^{\circ}C$ to 7.5$^{\circ}C$ for 15 to 60 days, $0^{\circ}C$ and 2.5$^{\circ}C$ showed the highest hatchability with 91% at 30 days and 61% at 60 days storage, respectively. 3. From these results, it can be concluded that by preserving 2 day-old eggs at 2.5$^{\circ}C$ for 50 to 60 days, rearing seasons required for preservation of the multivoltine silkworm can be reduced to half per year.

• Clinical and Experimental Pediatrics
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• v.52 no.6
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• pp.696-700
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• 2009

Purpose : We screened more than 350 compounds with an endoperoxide ring structure in search of an anti-leukemic drug and found that compound 127 (c-127) could induce significant cytotoxicity in HL-60 cells. In this study, we investigated the molecular mechanisms of compound 127-induced antitumor activity on HL-60 cells. Methods : HL-60 cells were cultured in Rosewell Park Memorial Institute 1640 and cell viability was measured by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], a tetrazole assay. Apoptosis was assessed by a DNA fragmentation test. Apoptotic machineries were determined by Western blot analysis. Results : C-127 could induce a cytotoxic effect at 24 h and apoptosis at 6 h, which was demonstrated with MTT assay and DNA fragmentation test, respectively. The apoptotic effect of this drug was caused by the activation of the intracellular caspase-8,3 activation, the cleavage of pro-apoptotic Bid, and the increase of c-Jun expression accompanied with JNK (Jun N-terminal kinases) phosphorylation. On the contrary, it increased the expression of anti-apoptotic Bcl-2 levels, leading to the induction of the induction of anti-apoptotic effect. Taken together, the present study demonstrated that c-127 was a potent inducer of cytotoxicity on HL-60 cells through apoptotic mechanisms, which included the activation of caspase family, the regulation of Bcl-2 family, and the activation of JNK signaling pathway. Conclusion : Our results suggest that c-127 has a strong antitumor activity through the regulation of various apoptotic machineries on HL-60 cells. The compound may be utilized as an effective and potentially therapeutic drug in leukemia.

• Textile Coloration and Finishing
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• v.5 no.4
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• pp.67-78
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• 1993

In one bath dyeing system of silk/PET fiber blend fabric with acid/disperse dyes, adsorption behavior of disperse dyes and acid dyes on silk and PET fabrics were examined. In the dyeing of PET with C.I.Disperse Red 19(Red 19) and C.I.Disperse Red 60(Red 60) at 10$0^{\circ}C$(carrier dyeing) and 13$0^{\circ}C$, dye uptake with Red 60 was higher than that with Red 19. When the silk/PET dyed with Red 19 and Red 60 at 10$0^{\circ}C$(carrier dyeing) and 13$0^{\circ}C$, dye uptake on PET was influenced by affinity of the dye to the silk fabric dyed together. When the silk/PET dyed with Blue 80/Red 19 and Blue 80/Red 60 at 10$0^{\circ}C$(carrier dyeing) and 13$0^{\circ}C$ for 60 minutes, color of PET dyed with Red 19 and Red 60 was little influenced by Blue 80 but silk dyed with Blue 80 was influenced. Interrelation of K/S value and Munsell value was scarcely any but showed the change tendency of K/S value.

• Applied Biological Chemistry
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• v.43 no.4
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• pp.247-252
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• 2000

As functionality investigation of Korean traditional soybean fermentation foods, an antihypertensive peptide was separated during Chunggugjang fermentation by Bacillus subtilis CH-1023 and investigated inhibitory effect against angiotensin converting enzyme. After incubation at $20^{\circ}C,\;30^{\circ}C,\;40^{\circ}C,\;50^{\circ}C,\;60^{\circ}C$ for the $0{\sim}72$ hrs, protein content, protease activity and angiotensin converting enzyme inhibitory rate were determined. The protein content and protease activity were increased and reached maximum at 60 hrs fermentation with $40^{\circ}C$ and decreased after the 60 hrs fermentation. The optimum condition for antihypertensive peptide from Chunggugjang was appeared for 60 hrs at $40^{\circ}C$. Crude extract of Chunggugjang was partially purified by Amicon YM-3 membrane filtration and Sephadex G-10, G-25 gel filtration. The purified peptide showed inhibitory rate of 94.3% with 0.5 mg peptide content. The most prominent amino acid composition of the peptide from Chunggugjang was alanine, followed by phenylalanine, histidine.

• Korean Journal of Food Science and Technology
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• v.43 no.3
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• pp.271-276
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• 2011

Volatile profiles of beverages and encapsulated powders containing Artemisia princeps Pampan extracts were analyzed by solid-phase microextraction-gas chromatography/mass spectrometry during production and storage. Beverages containing 0.32 and 0.64% extracts were stored at room temperature for 8 weeks and $60^{\circ}C$ for 8 days, respectively. Encapsulated particles were stored at room temperature and $60^{\circ}C$ for 8 days. Total volatiles in beverages decreased significantly during storage, irrespective of storage condition (p<0.05). Terpenoids, including cymene, thujone, and ${\beta}$-myrcene, were major volatiles in beverages, and some volatiles including ethylfuran, vinylfuran, and 2-fufural increased in 60oC samples only. Total volatiles in microcapsules at room temperature were not significant different for 8 days (p>0.05), whereas those at $60^{\circ}C$ increased by 16.5 times. Limonene was the most detected volatile in microcapsules, and aldehydes such as hexanal, pentanal, and octanal, and furans such as 2-butylfuran and 2-pentylfuran increased in the $60^{\circ}C$ samples, which may have originated from oxidized lipids used in the microcapsules.

• Food Science and Preservation
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• v.21 no.4
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• pp.506-511
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• 2014

In Gyeongsangbuk-do seongju area, hundreds of tons of spoiled oriental melon are harvested annually. Therefore, ways to prevent such spoilage are needed. This study was conducted to investigate the quality characteristics of spoiled oriental melon juice after enzyme treatment for the production of oriental melon concentrate. The treatment of the oriental melon juice with three kinds of enzymes with variable concentrations showed the following results. PECE(1), which was compounded pectinase and cellulase at 0.01% (v/v), gave the melon a 0.16 brown color and 0.01 turbidity, and the highest L value of 97.00. The detected free sugar contents were fructose, glucose and sucrose, with the amount of sucrose the highest at roughly 4,000 mg%. The mixture of different enzyme treatments resulted in a 0.15 brown color and 0.01 turbidity at 60 minutes, and the L value was high at 97.25. The enzyme treatment temperatures of $50^{\circ}C$ and $60^{\circ}C$ yielded a low-level brown color and low turbidity, but the L values were high at $60^{\circ}C$ and $70^{\circ}C$. These results showed that 0.01% (v/v) mixing enzyme, i.e., pectinase and cellulose compounded at $60^{\circ}C$ for 60 min, must be used for the production of oriental melon concentrate.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1617-1619
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• 2009

Lactobacillus rhamnosus PL60 was tested for whether it can produce c9,t11- and t10,c12-conjugated linoleic acids (CLAs) in human. After consumption of L. rhamnosus PL60, L. rhamnosus was detected in feces 1 week after the start of intake. Analysis by gas chromatography showed that concentrations of c9,t11- and t10,c12-CLAs in serum had increased and concentrations of serum leptin had significantly decreased. Results showed that L. rhamnosus PL60 can survive in human intestines and produce CLAs in humans. This is the first report that bacteria can produce CLAs in humans.