• Title/Summary/Keyword: $C_3F_6$

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Use of FT-IR to Identify Enhanced Biomass Production and Biochemical Pool Shifts in the Marine Microalgae, Chlorella ovalis, Cultured in Media Composed of Different Ratios of Deep Seawater and Fermented Animal Wastewater

  • Kim, Mi-Kyung;Jeune, Kyung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.19 no.10
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    • pp.1206-1212
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    • 2009
  • Growth rates, photosystem II photosynthesis, and the levels of chlorophyll $\alpha$ and secondary metabolites of Chlorella ovalis were estimated to determine if they were enhanced by the addition of swine urine (BM) or cow compost water (EP) that had been fermented by soil bacteria to deep seawater (DSW) in an attempt to develop media that enabled batch mass culture at lower costs. Growth of C. ovalis in f/2, f/2-EDTA+BM60%, DSW+BM30%, and DSW+EP60% was enhanced and maintained in the log phase of growth for 16 days. The cell densities of C. ovalis in DSW+EP60% ($4.1{\times}10^6$ Cells/ml) were higher than those of f/2 ($2.9{\times}10^6$ Cells/ml), f/2-E+BM60% ($3.7{\times}10^6$ Cells/ml), and DSW+BM30% ($2.7{\times}10^6$ Cells/ml). The growth rate was also more favorable for C. ovalis cultured in DSW+EP60% ($0.15\;day^{-1}$) than that of C. ovalis cultured in the control medium (f/2) ($0.12\;day^{-1}$). Furthermore, the chlorophyll a concentration of C. ovalis cultured in DSW+EP60% (4.56 mg/l) was more than 2-fold greater than that of C. ovalis cultured in f/2 (2.35 mg/l). Moreover, the maximal quantum yields of photo system II at 470 nm (Fv/Fm) were significantly higher in organisms cultured at f/2-E+BM60% (0.53) and DSW+EP60% (0.52) than in the other treatment groups. Finally, Fourier transformation infrared (FT-IR) spectroscopy revealed that C. ovalis grown in DSW+EP60% had more typical peaks and various biochemical pool shifts than those grown in other types of media. Taken together, the results of this study indicate that the use of DSW+EP60% to culture C. ovalis can reduce maintenance expenses and promote higher yields.

The Classification Algorithm of Users' Emotion Using Brain-Wave (뇌파를 활용한 사용자의 감정 분류 알고리즘)

  • Lee, Hyun-Ju;Shin, Dong-Il;Shin, Dong-Kyoo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39C no.2
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    • pp.122-129
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    • 2014
  • In this study, emotion-classification gathered from users was performed, classification-experiments were then conducted using SVM(Support Vector Machine) and K-means algorithm. Total 15 numbers of channels; CP6, Cz, FC2, T7. PO4, AF3, CP1, CP2, C3, F3, FC6, C4, Oz, T8 and F8 among 32 members of the channels measured were adapted in Brain signals which indicated obvious the classification of emotions in previous researches. To extract emotion, watching DVD and IAPS(International Affective Picture System) which is a way to stimulate with photos were applied and SAM(Self-Assessment Manikin) was used in emotion-classification to users' emotional conditions. The collected users' Brain-wave signals gathered had been pre-processing using FIR filter and artifacts(eye-blink) were then deleted by ICA(independence component Analysis) using. The data pre-processing were conveyed into frequency analysis for feature extraction through FFT. At last, the experiment was conducted suing classification algorithm; Although, K-means extracted 70% of results, SVM showed better accuracy which extracted 71.85% of results. Then, the results of previous researches adapted SVM were comparatively analyzed.

Antifungal Activities of Streptomyces blastmyceticus Strain 12-6 Against Plant Pathogenic Fungi

  • Kim, Yeon Ju;Kim, Jae-heon;Rho, Jae-Young
    • Mycobiology
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    • v.47 no.3
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    • pp.329-334
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    • 2019
  • Streptomyces blastmyceticus strain 12-6 was isolated from a forest soil sample of Cheonan area on the basis of strong antifungal activities against plant pathogenic fungi. Butanol extracts of the cultural filtrates were active against C. acutatum, C. coccodes, C. gloeosporioides, F. oxysporum, and T. roseum. Active fractions were prepared by thin layer chromatography using silica gel plate; 12-6-2 ($R_f$ 0.36), 12-6-3 ($R_f$ 0.44). Scanning electron microscopy showed that the active fractions caused a change in surface texture of fungal spores from smooth surface to wrinkled surface. The lethal effect on the spores of the active fractions varied from 56% to 100%. It was shown that the spores of C. acutatum were more sensitive to the antifungal fractions than the spores of F. oxysporum. Fluorescence staining using TOTO-1 indicated that the antifungal fractions could make the spores more sensitive to the fluorescence dye. Thus, it was suggested that antifungal agents prepared in this study exhibited the antifungal activity by damaging the plasma membrane of both fungal spores and hyphae. Identification of antifungal agents in the active fraction using GC-MS analysis revealed the presence of cyclo-(Leu-Pro) and 9-octadecenamide as major components that have already been known as antifungal substances.

Thermal and Mechanical Properties of Rapidly Solidified Zr-Ni-Cu-Al-Ti Alloy (급냉응고법으로 제조한 Zr-Ni-Cu-Al-Ti 합금의 열적, 기계적 성질)

  • Choe, Ik-Seok;Han, Tae-Gyo;Ji, Yong-Gwon;Im, Byeong-Mun;Kim, Yeong-Hwan;Kim, In-Bae
    • Korean Journal of Materials Research
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    • v.11 no.3
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    • pp.171-177
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    • 2001
  • The thermal and mechanical properties of amorphous Z $r_{62-x}$N $i_{10}$C $u_{20}$A $l_{8}$ $Ti_{x}$ (x=3, 6, 9at%) alloys were investigated. The crystallization process was confirmed as amorphous longrightarrow amorphous + Z $r_2$A $l_3$+ Zr + (Ni,Ti) longrightarrow Z $r_2$Cu + Al + (Ni,Ti) for 3at%Ti, amorphous longrightarrow amorphous + Al longrightarrow $Al_2$Ti + NiZr + CuTi for 6at%Ti and amorphous longrightarrow amorphous + Zr + Al longrightarrow Zr + $Al_2$Zr + Al $Ti_3$+ CuTi for 9at%Ti. lickers hardness ( $H_{v}$ ) increased with increasing volume fraction( $V_{f}$ ) of pricipitates for all concerned compositions. Tensile fracture strength ($\sigma_{f}$ ) showed a maximum value 1219MPa at $V_{f}$ = 38% for 3at%Ti, 1203MPa at $V_{f}$ = 2% for 6at%Ti and 1350MPa at $V_{f}$ = 5% for 9at%Ti. The $\sigma_{f}$ was rapidly decreased after showing the maximum value. The $V_{f}$ corresponding to rapidly decreased $\sigma_{f}$ coincided with the $V_{f}$ transited from ductile to brittle fracture surface.ace.

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Deposition of Super Hydrophobic a-C:F Films by Dielectric Barrier Discharge at Atmospheric Pressure

  • Kim, Duk-Jae;Kim, Yoon-Kee;Han, Jeon-Geon
    • Journal of Surface Science and Engineering
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    • v.44 no.2
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    • pp.50-54
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    • 2011
  • Hydrophobic a-C:F film was coated on polycarbonate film with $CF_4$, $C_2F_6$ and HFC ($C_2F_4H_2$) gas in helium discharge generated by 5~100 kHz AC power supply at atmospheric pressure and room temperature. The highest water contact angle of the a-C:F film formed with $He/C_2F_6$ mixed gas is $155^{\circ}$. X-ray photoelectron spectrum showed that there was 40% of C-$CF_3$ bond at the surface of the super hydrophobic film. The contact angle and deposition rate were decreased with increasing substrate temperature. The contact angle was generally increased with the surface roughness of the film. The contact angle was high when the surface microstructure of the film was fine and sharp at the similar roughness and chemical composition of the surface.

Effects of Adenosine and $N^6-cyclopentyladenosine$ on Superoxide Production, Degranulation and Calcium Mobilization in Activated Neutrophils (Adenosine과 $N^6-cyclopentyladenosine$이 활성화된 중성호성 백혈구에서 Superoxide 생성, 탈과립과 칼슘동원에 나타내는 영향)

  • Kim, Woo-Jung;Shin, Yong-Kyoo;Han, Eun-Sook;Lee, Chung-Soo
    • The Korean Journal of Pharmacology
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    • v.31 no.3
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    • pp.333-344
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    • 1995
  • The effects of adenosine and $N^6-cyclopentyladenosine$ (CPA) on superoxide production, myeloperoxidase release and $Ca^{2+}$ mobilization stimulated by fMLP in neutrophils were investigated. The effects were also observed on the stimulatory actions of C5a and PMA and the responses in lipopolysaccharide-primed neutrophils. In addition, the involvement of cAMP in the inhibitory action of adenosine was examined. The fMLP-stimulated neutrophil respiratory burst, degranulation and intracellular $Ca^{2+}$ mobilization may be regulated by activation of adenosine receptors. Adenosine may not affect the stimulated neutrophil responses due to activation of protein kinase C. fMLP-stimulated respiratory burst in lipopolysaccharide-primed neutrophils may be less sensitive to adenosine, compared with nonprimed cells. The inhibitory effect of theophylline in the presence of adenosine on neutrophil responses appears to be ascribed to accumulation of intracellular cAMP.

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Tourmaline Crystal Growth by FZ Method (FZ법에 의한 Tourmaline 단결정 성장)

  • 강승민;신재혁;한종원;최종건;오근호;박한수;문종수
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.3 no.1
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    • pp.31-36
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    • 1993
  • Na-Fe Tourmalene ($NaFe_3Al_6B_3Si_6O_{27}F_4$)single crystals were grown by FZ method. The Growth conditions are counter rotation 20~30rpm, growth rate 1~5mm/hr in the atmospheric environment. The transition elements as Fe(in this research), Mn, Co, etc, are exist in the theoretical fomulae so that dopants were not added. The feed rod was sintered between $1000^{\circ}C$ and $1l00^{\circ}C$, but at higher temperature, it was difficult to manufacture the feed rod because of evaporation of the elements such as F. As grown crystals had a black color and the length of 50~60mm, the diameter of 5~6mm. X-ray powder diffraction pattern indicated that the composition of the feed rod was right to the Tourmaline composition. By FT-IR spectra, the state of bonding of each element was charaterized.

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Reaction of Potassium Fluoride with Organic Halogen Compounds. (Part I) Reactions of Potassium Fluoride with Organic Halides, Acids, and Esters in presence of Dimethyl Formamide and their Pyrolytic Decaboxylation in presence of Potassium Fluoride (有機 할로겐 化合物과 弗化加里의 反應 (第1報) 有機 할라이드, 酸 및 에스테르와 弗化加里의 디메칠 호름아마이드 溶媒系反應 및 高溫-脫炭酸-熱分解反應)

  • You Sun Kim
    • Journal of the Korean Chemical Society
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    • v.7 no.3
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    • pp.189-196
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    • 1963
  • Reactions between potassium fluoride with organic halogen-containing carboxylic acids in dimethyl formamide solvent gave a decarboxylation reaction for the case of fluoro carboxylic acids of the type of $CF_3\;COOH,\;C_3F_7COOH,\;and\;C_2F_5COOH,$ whereas an additional partial fluorination together with dimerization reaction occurred for the chlorine containing acids of the type of $CH_2ClCOOH,\;CH_3CHClCOOH, \;CHCl_2COOH\;and\;o-Cl-C_6H_4-COOH.$ The phenyl halides showed no reactivity, but the halides with two electron attracting substituents on the benzene ring gave mainly dimerization reaction. The esters and alcohols gave an usual fluorination reaction. The same reactions in absence of the solvent at the elevated temperature increase the yield of the dimerized product and gave the cyclized product, fluorenone, in case of ο-chlorobenzoic acid. It was found that the fluorination usually precede the decarboxylation reaction by checking the stiochemical sequence of reaction. Catalytic influence of potassium fluoride were discussed and the mechanism of the reaction was considered.

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The Character of Electron Ionization and Attachment Coefficients in Perfluoropropane(C3F8) Molecular Gas by the Boltzmann Equation (볼츠만 방정식에 의한 C3F8분자가스의 전리 및 부착 계수에 관한 연구)

  • Song, Byoung-Doo;Jeon, Byoung-Hoon;Ha, Sung-Chul
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.18 no.4
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    • pp.375-380
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    • 2005
  • CF₄ molecular gas is used in most of semiconductor manufacture processing and SF/sub 6/ molecular gas is widely used in industrial of insulation field. but both of gases have defect in global warming. C₃F/sub 8/ gas has large attachment cross-section more than these gases, moreover GWP, life-time and price of C₃F/sub 8/ gas is lower than them, therefor it is important to calculate transport coefficients of C₃F/sub 8/ gas like electron drift velocity, ionization coefficient, attachment coefficient, effective ionization coefficient and critical E/N. The aim of this study is to get these transport coefficients for imformation of the insulation strength and efficiency of etching process. In this paper, we calculated the electron drift velocity (W) in pure C₃F/sub 8/ molecular gas over the range of E/N=0.1∼250 Td at the temperature was 300 K and gas pressure was 1 Torr by the Boltzmann equation method. The results of this paper can be important data to present characteristic of gas for plasma etching and insulation, specially critical E/N is a data to evaluate insulation strength of a gas.

A Study on analysis of contrasts and variation in SUV with the passage of uptake time in 18F-FDOPA Brain PET/CT (18F-FDOPA Brain PET/CT 검사의 영상 대조도 분석 및 섭취 시간에 따른 SUV변화 고찰)

  • Seo, Kang rok;Lee, Jeong eun;Ko, Hyun soo;Ryu, Jae kwang;Nam, Ki pyo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.23 no.1
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    • pp.69-74
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    • 2019
  • Purpose $^{18}F$-FDOPA using amino acid is particularly attractive for imaging of brain tumors because of the high uptake in tumor tissue and the low uptake in normal brain tissue. But, on the other hand, $^{18}F$-FDG is highly uptake in both tumor tissue and normal brain tissue. The purpose of study is to evaluate comparison of contrasts in $^{18}F$-FDOPA Brain PET/CT and $^{18}F$-FDG Brain PET/CT and to find out optimal scan time by analysis of variation in SUV with the passage of uptake time. Materials and Methods A region of interest of approximately $350mm^2$ at the center of the tumor and cerebellum in 12 patients ($51.4{\pm}12.8yrs$) who $^{18}F$-FDG Brain PET/CT and $^{18}F$-FDOPA Brain PET/CT were examined more than once each. The $SUV_{max}$ was measured, and the $SUV_{max}$ ratio (T/C ratio) of the tumor cerebellum was calculated. In the analysis of SUV, T/C ratio was calculated for each frame after dividing into 15 frames of 2 minutes each using List mode data in 25 patients ($49.{\pm}10.3yrs$). SPSS 21 was used to compare T/C ratio of $^{18}F$-FDOPA and T/C ratio of $^{18}F$-FDG. Results The T/C ratio of $^{18}F$-FDOPA Brain PET/CT was higher than the T/C ratio of $^{18}F$-FDG Brain, and show a significant difference according to a paired t-test(t=-5.214, p=0.000). As a result of analyzing changes in $SUV_{max}$ and T/C ratio, the peak point of $SUV_{max}$ was $5.6{\pm}2.9$ and appeared in the fourth frame (6 to 8 minutes), and the peak of T/C ratio also appeared in the fourth frame (6 to 8 minutes). Taking this into consideration and comparing the existing 10 to 30 minutes image and 6 to 26 minutes image, the $SUV_{max}$ and T/C ratio increased by 0.2 and 0.1 each, compared to the 10 to 30 minutes image for 6 to 26 minutes image. Conclusion From this study, $^{18}F$-FDOPA Brain PET/CT is effective when reading the image, because the T/C ratio of $^{18}F$-FDOPA Brain PET/CT was higher than T/C ratio of $^{18}F$-FDG Brain PET/CT. In addition, in the case of $^{18}F$-FDOPA Brain PET/CT, there was no difference between the existing 10 to 30 minutes image and 6 to 26 minutes image. Through continuous research, we can find possibility of shortening examination time in $^{18}F$-FDOPA Brain PET/CT. Also, we can help physician to accurate reading using additional scan data.