Browse > Article
http://dx.doi.org/10.1080/12298093.2019.1635425

Antifungal Activities of Streptomyces blastmyceticus Strain 12-6 Against Plant Pathogenic Fungi  

Kim, Yeon Ju (Department of Microbiology, College of Natural Sciences, Dankook University)
Kim, Jae-heon (Department of Microbiology, College of Natural Sciences, Dankook University)
Rho, Jae-Young (Department of Microbiology, College of Natural Sciences, Dankook University)
Publication Information
Mycobiology / v.47, no.3, 2019 , pp. 329-334 More about this Journal
Abstract
Streptomyces blastmyceticus strain 12-6 was isolated from a forest soil sample of Cheonan area on the basis of strong antifungal activities against plant pathogenic fungi. Butanol extracts of the cultural filtrates were active against C. acutatum, C. coccodes, C. gloeosporioides, F. oxysporum, and T. roseum. Active fractions were prepared by thin layer chromatography using silica gel plate; 12-6-2 ($R_f$ 0.36), 12-6-3 ($R_f$ 0.44). Scanning electron microscopy showed that the active fractions caused a change in surface texture of fungal spores from smooth surface to wrinkled surface. The lethal effect on the spores of the active fractions varied from 56% to 100%. It was shown that the spores of C. acutatum were more sensitive to the antifungal fractions than the spores of F. oxysporum. Fluorescence staining using TOTO-1 indicated that the antifungal fractions could make the spores more sensitive to the fluorescence dye. Thus, it was suggested that antifungal agents prepared in this study exhibited the antifungal activity by damaging the plasma membrane of both fungal spores and hyphae. Identification of antifungal agents in the active fraction using GC-MS analysis revealed the presence of cyclo-(Leu-Pro) and 9-octadecenamide as major components that have already been known as antifungal substances.
Keywords
Antifungal activity; Streptomyces; plant pathogenic; spore; hyphae;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Oerke EC, Dehne HW, Schonbeck F, et al. Crop production and crop protection: estimated losses in major food and cash crops. 1st ed. Amsterdam: Elsevier; 1994.
2 Cook RJ. Biological control of plant pathogens: theory to application. Phytopathology. 1985;75:25-28.   DOI
3 Ames BN. Identifying environmental chemicals causing mutations and cancer. Science. 1979;204:587-593.   DOI
4 Staub T, Sozzi D. Fungicide resistance: a continuing challenge. Plant Dis. 1984;68:1026-1031.   DOI
5 Huang TC, Chang MC. Studies on xanthobacidin, a new antibiotic from Bacillus subtilis active against Xanthomonas. Bot Bull Acad Sinica. 1975;16:137-148.
6 Yoshikawa Y, Ikai K, Umeda Y, et al. Isolation, structures, and antifungal activities of new aureobasidins. J Antibiot. 1993;46:1347-1354.   DOI
7 Cha MS, Lim EG, Lee KH, et al. Optimal culture conditions for production of environment-friendly biosurfactant by Pseudomonas sp. EL-G527. J Environ Sci. 2002;11:177-182.
8 Jo YK, Chang SW, Boehm M, et al. Rapid development of fungicide resistance by Sclerotinia homoeocarpa on turfgrass. Phytopathology. 2008;98:1297-1304.   DOI
9 Scherwinski K, Grosch R, Berg G. Effect of bacterial antagonists on lettuce: active biocontrol of Rhizoctonia solani and negligible, short-term effects on non-target microbes. FEMS Microbiol Ecol. 2008;64:106-116.   DOI
10 Koch E, Loffler I. Partial characterization of the antimicrobial activity of Streptomyces antimycoticus FZB53. J Phytopathol. 2009;157:235-242.   DOI
11 Van Leeuwen MR, Van Doorn TM, Golovina EA, et al. Water- and air-distributed conidia differ in sterol content and cytoplasmic microviscosity. Appl Environ Microbiol. 2010;76:366-369.   DOI
12 Jenssen H, Hamill P, Hancock R. Peptide antimicrobial agents. Clin Microbiol Rev. 2006;19:491-511.   DOI
13 Selvin J, Shanmughapriya S, Gandhimathi R, et al. Optimization and production of novel antimicrobial agents from sponge associated marine actinomycetes Nocardiopsis dassonvillei Mad08. Appl Microbiol Biotechnol. 2009;83:435-445.   DOI
14 Welscher YM, Napel HH, Balague MM, et al. Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. J Biol Chem. 2008;283:6393-6401.   DOI
15 Donio MBS, Ronica FA, Thanga Viji V, et al. Halomonas sp. BS4, a biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance. Springerplus. 2013;2:149-159.   DOI
16 Rhee K-H. Purification and identification of an antifungal agent from Streptomyces sp. KH-614 antagonistic to rice blast fungus, Pyricularia oryzae. J Microbiol Biotechnol. 2003;13:984-988.
17 Rhee K-H. Cyclic dipeptides exhibit synergistic, broad spectrum antimicrobial effects and have anti-mutagenic properties. Int J Antimicrob Agents. 2004;24:423-427.   DOI
18 Li H, Liu L, Zhang S, et al. Identification of antifungal compounds produced by Lactobacillus casei AST18. Curr Microbiol. 2012;65:156-161.   DOI
19 Kwak M-K, Liu R, Kim M-K, et al. Cyclic dipeptides from lactic acid bacteria inhibit the proliferation of pathogenic fungi. J Microbiol. 2014;52:64-70.   DOI
20 Liu R, Kim AH, Kwak M-K, et al. Proline-based cyclic dipeptides from Korean fermented vegetable kimchi and from Leuconostoc mesenteroides LBPK06 have activities against multidrug-resistant bacteria. Front Microbiol. 2017;8:761.   DOI
21 Mika JT, Moiset G, Cirac AD, et al. Structural basis for the enhanced activity of cyclic antimicrobial peptides: the case of BPC194. Biochim Biophys Acta. 2011;1808:2197-2205.   DOI