• Title/Summary/Keyword: $C_2Cl_F_2$

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Protoplast Fusion Between Aspergillus oryzae and Aspergillus shirousamii (Aspergillus oryzae와 Aspergillus shirousamii간의 원형질체의 융합)

  • Shin, Dong-Bun;Ryu, Beung-Ho;Jin, Seung-Heun
    • Korean Journal of Food Science and Technology
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    • v.25 no.4
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    • pp.366-372
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    • 1993
  • This study mainly designed to high quality of mirin production by using protopast fusion. In order to enhance the acid carboxypeptidase (ACPase) activity by the method of protoplast fusion. In order to enhance the acid carboxypeptidase (ACPase) activity by the method of protopalst fusion, the mutants, Aspergillus oryzae 9-12 and Aspergillus shirosamii IFO 6082-60 were selected by mutation among various mutants. Protoplast of Aspergillus oryzae 9-12 and Aspergillus shirousamii IFO 6082-60 were formed effectively by incubation of the mixtures of chitinase (10mg/ml), cellulase (10mg/ml) and zymolase 20T (5mg/ml). For protopalst fusion, the mixture of two mutant were fused to effective under the optimum conditions by solutions containing 30% PEG 6,000, 0.01M $CaCl_2\;2H_2O$, 0.6M KCl and 0.05M glycine. Fusion frequency was 0.71% and fusant, F-50 appeared ACPase activity of 20,800 unit/g which has 1.5 times higher than that of each mutants.

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Photoelectrochemical and Hydrogen Production Characteristics of CdS-TiO2 Nanocomposite Photocatalysts Synthesized in Organic Solvent (유기용매상에서 제조된 수소제조용 CdS-TiO2 나노복합 광촉매의 특성 연구)

  • Jang, Jum-Suk;So, Won-Wook;Kim, Kwang-Je;Moon, Sang-Jin
    • Journal of Hydrogen and New Energy
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    • v.13 no.3
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    • pp.224-232
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    • 2002
  • CdS-$TiO_2$ nano-composite sol was prepared by the sol-gel method in organic solvents at room temperature and further hydrothermal treatment at various temperatures to control the physical properties of the primary particles. Again, CdS-$TiO_2$ composite particulate films were made by casting CdS-$TiO_2$ sols onto $F:SnO_2$ conducting glass and then heat-treatment at $400^{\circ}C$. Physical properties of these 61ms were further controlled by the surface treatment with $TiCl_4$, aqueous solution. The photo currents and hydrogen production rates measured under the experimental conditions varied according to the $CdS/[CdS+TiO_2]$ mole ratio and the mixed-sol preparation method. For $CdS-TiO_2$ composite sols prepared in IPA, CdS particles were homogeneously surrounded by $TiO_2$ particles. Also, the surface treatment with $TiCl_4$ aqueous solution caused a considerable improvement in the photocatalytic activity, probably as a result of close contacts between the primary particles by the etching effect of $TiCl_4$. It was found that the photoelectrochemical performance of these particulate films could be effectively enhanced by this approach.

Electrochemical Studies on the Reaction of Superoxide Ion with Halocarbons in Aprotic Media

  • Jeon, Seungwon;Choi, Yong-Kook
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.649-654
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    • 1995
  • The reactivity of superoxide ion($O{_2}^{-.}$) with halogenated substrates is investigated by cyclic voltammetry and rotated ring-disk electrode method in aprotic solvents. The more positive the reduction potential of the substituted nitrile, the more facile is nucleophilic displacement by $O{_2}^{-.}$. The reaction rates of halogenonitriles with $O{_2}^{-.}$ vary according to the leaving-group propensity of halide (Br>Cl>F). The relative reaction rates of other substituted nitriles are in the order of electron-withdrawing propensity of the substituent group (CN> $C(O)NH_2$ >Ph, $CH_2CN$). The reaction of $O{_2}^{-.}$ with dihalocarbons indicates that five-membered rings can be rapidly formed by the cyclization of substrate and $O{_2}^{-.}$, and the relative rates of cyclization depend on the number of methylenic carbons {$Br(CH_2)_nBr$, [n=1<2<3>4>5]}. Mechanisms are proposed for the reaction of $O{_2}^{-.}$ with halogenated substrates.

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Reactivity of Superoxide Ion with Halogenonitriles and Dihalocarbons in Aprotic Media

  • 전승원;최용국
    • Bulletin of the Korean Chemical Society
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    • v.16 no.11
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    • pp.1060-1064
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    • 1995
  • The reactivity of superoxide ion (O2-.) with halogenated substrates is investigated by cyclic voltammetry and rotated ring-disk electrode method in aprotic solvents. The more positive the reduction potential of the substituted nitrile, the more facile is nucleophilic displacement by O2-.. The reaction rates of halogenonitriles with O2-. vary according to the leaving-group propensity of halide (Br > Cl > F). The relative reaction rates of other substituted nitriles are in the order of electron-withdrawing propensity of the substituent group (CN > C(O)NH2 > Ph ≒ CH2CN). The reaction of O2-. with dihalocarbons indicates that five-membered rings can be rapidly formed by the cyclization of substrate and O2-., and the relative rates of cyclization depend on the number of methylenic carbons {Br(CH2)nBr, [n=1 < 2 < 3 > 4 > 5]}. Mechanisms are proposed for the reaction of O2-. with halogenated substrates.

Common Fixed Point Theorems of Commuting Mappinggs

  • Park, Wee-Tae
    • The Mathematical Education
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    • v.26 no.1
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    • pp.41-45
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    • 1987
  • In this paper, we give several fixed point theorems in a complete metric space for two multi-valued mappings commuting with two single-valued mappings. In fact, our main theorems show the existence of solutions of functional equations f($\chi$)=g($\chi$)$\in$S$\chi$∩T$\chi$ and $\chi$=f($\chi$)=g($\chi$)$\in$S$\chi$∩T$\chi$ under certain conditions. We also answer an open question proposed by Rhoades-Singh-Kulsherestha. Throughout this paper, let (X, d) be a complete metric space. We shall follow the following notations : CL(X) = {A; A is a nonempty closed subset of X}, CB(X)={A; A is a nonempty closed and founded subset of X}, C(X)={A; A is a nonempty compact subset of X}, For each A, B$\in$CL(X) and $\varepsilon$>0, N($\varepsilon$, A) = {$\chi$$\in$X; d($\chi$, ${\alpha}$) < $\varepsilon$ for some ${\alpha}$$\in$A}, E$\sub$A, B/={$\varepsilon$ > 0; A⊂N($\varepsilon$ B) and B⊂N($\varepsilon$, A)}, and (equation omitted). Then H is called the generalized Hausdorff distance function fot CL(X) induced by a metric d and H defined CB(X) is said to be the Hausdorff metric induced by d. D($\chi$, A) will denote the ordinary distance between $\chi$$\in$X and a nonempty subset A of X. Let R$\^$+/ and II$\^$+/ denote the sets of nonnegative real numbers and positive integers, respectively, and G the family of functions ${\Phi}$ from (R$\^$+/)$\^$s/ into R$\^$+/ satisfying the following conditions: (1) ${\Phi}$ is nondecreasing and upper semicontinuous in each coordinate variable, and (2) for each t>0, $\psi$(t)=max{$\psi$(t, 0, 0, t, t), ${\Phi}$(t, t, t, 2t, 0), ${\Phi}$(0, t, 0, 0, t)} $\psi$: R$\^$+/ \longrightarrow R$\^$+/ is a nondecreasing upper semicontinuous function from the right. Before sating and proving our main theorems, we give the following lemmas:

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Production and Characteristics of Environment-Friendly Antimicrobial Substance by Pseudomonas aeruginosa EL-KM (Pseudomonas aeruginosa EL-KM에 의한 환경친화적 항균물질의 생산과 특성)

  • 이상준;이경민;이오미;차미선;박은희;박근태;손홍주
    • Journal of Environmental Science International
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    • v.11 no.1
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    • pp.33-40
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    • 2002
  • An antimicrobial substance-producing microorganism was isolated from soil samples. Based of the taxonomic characteristics of its morphological, cultural, physiological properties and 16s rRNA sequence alignment, this microorganism was identified as Pseudomonas aeruginosa, and we named Pseudomonas aeruginosa EL-KM. The optimal culture condition for production of antimicrobial substance was 1% mannitol, 0.4% yeast extract, 0.5% Nacl, 0.2% $K_2SO_4$, 100$\mu$M $MgSO_4$.$7H_2O$, 10$\mu$M $CaCl_2$.$2H_2O$, 1$\mu$M $FeSO_4$.$7H_2O$, 1$\mu$M $MnSO_4$.$4-5H_2O$, initial pH 7 and 200 rpm at 3$0^{\circ}C$. The purification of the antimicrbial substance was performed by silica gel column chromatographys, and fraction with TLC $R_f$ 0.77 value represented good antimicrobial activity. The crude antimicrbial substance was stable within a pH range of 3-10 and temperature range of 4$^{\circ}C$-121$^{\circ}C$ autoclaving. This crude antibacterial substance acted as bacteriolytic agent against Vibrio cholerae non-Ol ATCC 25872, and also exhibited excellent properties, when the substance was demonstrated against many other gram-positive, gram-negative bacteria, yeast and fungi.

Optimal Conditions for Antimicrobial Metabolites Production from a New Streptomyces sp. RUPA-08PR Isolated from Bangladeshi Soil

  • Ripa, F.A.;Nikkon, F.;Zaman, S.;Khondkar, P.
    • Mycobiology
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    • v.37 no.3
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    • pp.211-214
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    • 2009
  • An actinomycete strain was isolated from northern part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. Attempts were made to optimize the culture conditions for the production of antimicrobial metabolites by this strain. Antimicrobial metabolites production was started after 7 days of incubation of culture broth and reached its maximum levels after 10 days and thereafter gradually decreased. The maximum production of antimicrobial metabolites was obtained when the culture medium pH was adjusted to 8. The optimum temperature for antimicrobial metabolites production was $39^{\circ}C$, indicated the new strain as mesophilic organism. Basel medium supplemented with glucose and yeast extract as carbon and nitrogen sources, respectively, was proved to be the best for the production of bioactive metabolites. Maximum production of bioactive metabolites was when NaCl concentration was 1% and among different minerals tested, $K_2HPO_4$ and NaCl showed positive influence on antibiotic production by the strain.

Studies on Fluid Inclusion and Stable Isotope of the Namsan Gold-Silver Mine (남산 금-은광산의 유체포유물 및 안정동위원소 연구)

  • Kim, Se-Hyun;Choi, Sang-Hoon
    • Economic and Environmental Geology
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    • v.29 no.2
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    • pp.119-127
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    • 1996
  • Electrum-sulfide minerals of the Namsan Au-Ag mine were deposited in two stages of quartz and calcite veins that fill fault planes in Mesozoic granitic rocks (230~155 Ma). The K/Ar radiometric dating of hydrothermal sericite indicates that mineralization is early Cretaceous age ($127{\pm}3.0Ma$). Mineralogic, fluid inclusion and sulfur isotopic data show that ore minerals were deposited at temperatures between $340^{\circ}C$ and $200^{\circ}C$ from fluid with salinities of 3 to 6 equiv. wt % NaCl. Evidence of fluid boiling (and $CO_2$ effervescence) indicates a maximum pressures of 100 bars. The formation temperature and $fs_2$, of Au-mineralization from the Namsan mines are mainly $280{\sim}230^{\circ}C$ and $10^{-11}{\sim}10^{-13}$ atm, respectively. Au deposition was likely a result of boiling caused to chemical change (pH, $f_{O2}$, ${\Sigma}_{H_2S{\cdot}{\cdot}}$) of ore-fluids. Sulfur isotope composition of sulfide minerals (${\delta}^{34}S=5.1$ to 8.2‰) are consistent with ${\delta}^{34}S_{{\Sigma}{H2S}}$ value of +6 to +7‰, suggesting an igneous source of sulfur partially mixed with wall-rock sulfur.

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Lamellar Structured TaN Thin Films by UHV UBM Sputtering (초고진공 UBM 스퍼터링으로 제조된 라멜라 구조 TaN 박막의 연구)

  • Lee G. R.;Shin C. S.;Petrov I.;Greene J, E.;Lee J. J.
    • Journal of Surface Science and Engineering
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    • v.38 no.2
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    • pp.65-68
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    • 2005
  • The effect of crystal orientation and microstructure on the mechanical properties of $TaN_x$ was investigated. $TaN_x$ films were grown on $SiO_2$ substrates by ultrahigh vacuum unbalanced magnetron sputter deposition in mixed $Ar/N_2$ discharges at 20 mTorr (2.67 Pa) and at $350^{\circ}C$. Unlike the Ti-N system, in which TiN is the terminal phase, a large number of N-rich phases in the Ta-N system could lead to layers which had nano-sized lamella structure of coherent cubic and hexagonal phases, with a correct choice of nitrogen fraction in the sputtering mixture and ion irradiation energy during growth. The preferred orientations and the micro-structure of $TaN_x$ layers were controlled by varing incident ion energy $E_i\;(=30eV\~50eV)$ and nitrogen fractions $f_{N2}\;(=0.1\~0.15)$. $TaN_x$ layers were grown on (0002)-Ti underlayer as a crystallographic template in order to relieve the stress on the films. The structure of the $TaN_x$ film transformed from Bl-NaCl $\delta-TaN_x$ to lamellar structured Bl-NaCl $\delta-TaN_x$ + hexagonal $\varepsilon-TaN_x$ or Bl-NaCl $\delta-TaN_x$ + hexagonal $\gamma-TaN_x$ with increasing the ion energy at the same nitrogen fraction $f_{N2}$. The hardness of the films also increased by the structural change. At the nitrogen fraction of $0.1\~0.125$, the structure of the $TaN_x$ films was changed from $\delta-TaN_x\;+\;\varepsilon-TaN_x\;to\;\delta-TaN_x\;+\;\gamma-TaN_x$ with increasing the ion energy. However, at the nitrogen fraction of 0.15 the film structure did not change from $\delta-TaN_x\;+\;\varepsilon-TaN_x$ over the whole range of the applied ion energy. The hardness increased significantly from 21.1 GPa to 45.5 GPa with increasing the ion energy.

The Effect of Substituent, Pressure and Temperature on the Dissociation Constants of Organic Acids. (2) Dissociation Constants of Some Substituted Naphthols in Aqueous Solution (유기산의 해리평형에 미치는 치환기 효과와 그의 온도 및 압력의 영향. (2) 수용액중에서 몇가지 치환나프톨류의 해리상수)

  • Jung-Ui Hwang;Zun-Ung Bae;Jong-Jae Chung;Jae-Won Jung;Kyung-Hee Chang
    • Journal of the Korean Chemical Society
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    • v.30 no.2
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    • pp.152-158
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    • 1986
  • The dissociation constants of 4-Cl-1-naphthol, 6-Br-2-naphthol and $8-NH_2-2-naphthol$ in aqueous solution were measured by spectroscopic method in the temperature range from 25 to 40${\circ}C$ and pressure up to 2000bar. The dissociation constants were decreased as the substituents were inserted in naphthol f rom $4.4{\times}10^{-10}\;to\;5.82{\times}10^{-11}$ as chloride compound and $2.5{\times}10^{-10}\;to\;3.44{\times}10^{-11}\;or\;4.21{\times}10^{-11}$ as bromine or amino compounds, respectively. This decrease can be explained with the I-or R-effects of substituents. From the dissociation constants various thermodynamic properties were calculated and discussed the characteristics of the dissociation reaction.

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