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http://dx.doi.org/10.5322/JES.2002.11.1.033

Production and Characteristics of Environment-Friendly Antimicrobial Substance by Pseudomonas aeruginosa EL-KM  

이상준 (부산대학교 미생물학과)
이경민 (부산대학교 미생물학과)
이오미 (부산대학교 미생물학과)
차미선 (부산대학교 미생물학과)
박은희 (부산광역시 보건환경연구원)
박근태 (부산대학교 미생물학과)
손홍주 (밀양대학교 생물공학과)
Publication Information
Journal of Environmental Science International / v.11, no.1, 2002 , pp. 33-40 More about this Journal
Abstract
An antimicrobial substance-producing microorganism was isolated from soil samples. Based of the taxonomic characteristics of its morphological, cultural, physiological properties and 16s rRNA sequence alignment, this microorganism was identified as Pseudomonas aeruginosa, and we named Pseudomonas aeruginosa EL-KM. The optimal culture condition for production of antimicrobial substance was 1% mannitol, 0.4% yeast extract, 0.5% Nacl, 0.2% $K_2SO_4$, 100$\mu$M $MgSO_4$.$7H_2O$, 10$\mu$M $CaCl_2$.$2H_2O$, 1$\mu$M $FeSO_4$.$7H_2O$, 1$\mu$M $MnSO_4$.$4-5H_2O$, initial pH 7 and 200 rpm at 3$0^{\circ}C$. The purification of the antimicrbial substance was performed by silica gel column chromatographys, and fraction with TLC $R_f$ 0.77 value represented good antimicrobial activity. The crude antimicrbial substance was stable within a pH range of 3-10 and temperature range of 4$^{\circ}C$-121$^{\circ}C$ autoclaving. This crude antibacterial substance acted as bacteriolytic agent against Vibrio cholerae non-Ol ATCC 25872, and also exhibited excellent properties, when the substance was demonstrated against many other gram-positive, gram-negative bacteria, yeast and fungi.
Keywords
Antimicrobial substance; Pseudomonas aeruginosa EL-KM; Vibrio;
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