• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
• /
• pp.137-137
• /
• 1998

Stability, efficacy, absorption and toxicity of a new nasal spray formulation including salmon calcitonin were studied in the laboratory animals. After the effects of many excipients on the stability of salmon calcitonin were evaluated using HPLC system, we selected taurine. Our experimental composition of salmon calcitonin contains taurine as a stabilizer and HPMC (hydroxypropylmethyl cellulose) as an adhesive polymer. After intranasal administration of salmon calcitonin formulations, Mia$\^$(R)/, Men$\^$(R)/ and experimental composition, 22 IU to rats, the reduction percentages of calcium concentration in plasma (ΔD%) were 16.3%, 12.9% and 20.8%, respectively. After intranasal administration of Mia$\^$(R)/, Men$\^$(R)/ and experimental composition to rats, C$\sub$MAX/ (205${\pm}$161, 244${\pm}$117, and 330${\pm}$202 pg/$m\ell$, respectively) and AUC (41585${\pm}$22070, 41191${\pm}$19125, and 63357${\pm}$43126 pg. min/$m\ell$, respectively) were calculated. The permeation coefficients 10$\^$-7/,cm/sec) of salmon calcitonin in Mia$\^$(R)/, Men$\^$(R)/ and experimental composition using Ussing chamber with rabbit nasal mucosa were 4.7${\pm}$1.5, 0.75${\pm}$0.4 and 5.3${\pm}$1.1, respectively. The experimental composition with taurine and HPMC was proved to be excellent because it improved the stability of salmon calcitonin and inhanced the absorption of salmon calcitonin and was not irritative to the nasal mucosa.

• Plant Biotechnology Reports
• /
• 제4권1호
• /
• pp.23-27
• /
• 2010

Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

• 한국잡초학회지
• /
• 제15권4호
• /
• pp.313-320
• /
• 1995

논을 밭으로 전환(轉換)하여 콩을 파종 재배하였을 때 잡초발생동태 및 효과적인 방제체계를 확립하기 위하여 수행한 일련의 시험결과를 요약하면 다음과 같다. 1. 밭으로 전환시(轉換時) 주요 발생 잡초는 (좀)바랭이, 피, 좀명아주, 속속이풀, 벼룩나물 등이었으며 첫해보다는 2년째에 광엽잡초 특히, 좀명아주, 속속이풀의 발생량이 크게 증가하였으며, 4월 26일 파종구에 비하여 5월 20일에 파종했을 때 잡초의 발생량이 84.8%(건물중 기준) 감소되었다. 2. 무방제(無除草) 방임(放任) 상태(狀態)에서는 콩의 분지수, 주경의 지름, 꼬투리수에 크게 영향을 미치고, 콩의 수량이 70% 감소되었다. 3. 답전윤환 콩재배지에서는 pendimethalin, metolachlor, metolachlor+prometryn, alachlor 처리로 효과적인 잡초방제가 가능하였고 그 중에 metolachlor+prometryn가 가장 우수하였다.

• Journal of Pharmaceutical Investigation
• /
• 제30권1호
• /
• pp.21-26
• /
• 2000

Bioavailability of ipriflavone (3-phenyl-7-isopropoxy-4H-I-benzopyran-4-one, IP), an antiosteoporotic drug with poor water-solubility, was studied for various types of pharmaceutical preparation in SD rats. The IP preparation types included (1) intact IP, (2) freezer milled IP (FIP), (3) freezer milled IP physically mixed with freezer milled poly-N-vinylpyrrolidone (PVP) (FIP+FPVP) and (4) spray-dried IP with PVP (SIP). Upon oral administration, SIP showed significantly higher absorption and elimination half-lives and the lag time $(t_{lag})$ than those of FIP+FPVP (approximately 2-fold). These results may be due to a sustained releasing effect of IP in the gastrointestinal tract by spray-drying with PVP. The $C_{max}$ of SIP was about 2 and 10 times higher than those of FIP+FPVP and FIP, respectively. The AUC of SIP was about 6 times higher than that of FIP+FPVP and 60 times that of FIP. Scanning electron microscopy (SEM) showed that SIP consisted of the finest particle size and minimal aggregation than other IP preparations. It is concluded that the IP formula prepared by the spray-drying method with PVP is the most effective approach to the improvement of bioavailability of IP.

• Journal of Microbiology and Biotechnology
• /
• 제15권2호
• /
• pp.274-280
• /
• 2005

A chitinase was purified from the culture supernatant of Paenibacillus illinoisensis KJA-424 by protein precipitation, DEAE-Sephadex anion-exchange chromatography, and Sephadex G-150 gel filtration. The molecular weight of the purified chitinase was 54 kDa on SDS-PAGE and activity staining. Optimal pH and temperature were pH 5.0 and 60$^{circ}$C, the presence of 10 ruM Ag$^{+}$ and Hg$^{2+}$ inhibited the activity by $92.1/%$ and $97.7/%$, and the K$_{m}$ and V$_{max}$ values were 1.12 mg chitin mrl and 1.48$\mu$mol GlcNAc min$^{-1}$, respectively. The enzyme hydrolyzed tetramer to dimer, pentamer to dimer and trimer, and hexamer to dimer, trimer and tetramer, indicating an endo-splitting mechanism. The chitinase had no hydrolytic activity toward dimer and trimer. The chitinase inhibited the mycelial growth of Rhizoctonia solani, suggesting an antifungal property.

• 한국작물학회지
• /
• 제58권3호
• /
• pp.260-266
• /
• 2013

The study was carried out to determine the gel pasting properties of barley (Hordeum vulgare L. cv. Geoncheonheugbori) as affected by different proton beam irradiation. The ${\lambda}max$, blue value, and amylose content were significantly associated with increasing proton beam irradiation. The pasting time in barley flour irradiated with proton beam ranged 0.09 to 0.16 min shorter than nonirradiated barley flour. Gel pasting temperature ranged 57.4 to $60.5^{\circ}C$. Gel pasting temperature in barley flour decreased with increasing proton beam irradiation. Proton beam irradiation caused a significant decrease in the onset temperature (To), peak temperature (Tp), conclusion temperature (Tc) and enthalpy change (${\Delta}H$). Gelatinization range (R) in barley starch was more broaden than that of non-irradiated barley starch. Barley starches gave the strong diffraction peak at around $2{\Theta}$ values$15^{\circ}$, $18^{\circ}$, $20^{\circ}$, and $23^{\circ}$ $2{\Theta}$. Peak intensity tended to increase with increased proton beam irradiation. The granule crystallinity is closely associated with decreased amylose and increased amylopectin component. The crystallinity degree of barley starch irradiated with proton beam was significantly increased and it ranged from 24.9 to 32.9% compared to the non-irradiated barley starches. It might be deduced that proton beam irradiation causes significant changes of properties of starch viscosity in rice, especially at high irradiation of proton beam.

• 한국염색가공학회지
• /
• 제18권2호
• /
• pp.8-14
• /
• 2006

The pigment extraction of Opuntia ficus-indica has been conducted to develop useful natural dyes in place of synthetic dyes which are suspected to bring serious environmental pollutions. The dyeing ability on wool fabric by addition of ascorbic acid and several mordants were investigated by means of color measurement. In addition, the fastness of washing, perspiration, rubbing, light, dry cleaning, effect on bacterial reduction and UV-B protection were also investigated. From these investigation, it is suggested that the pigment extracted from Opuntia ficus-indica can be used as a source of natural dyes and the obtained result are as follows. 1. Maximum absorption band (${\lambda}max$) of Opuntia ficus-indica extract is 533nm. 2. The wool fabric dyed with Opuntia ficus-indica extract has stable color by the addition of ascorbic acid and is achieved with addition of 0.1% ascorbic acid, 0.5% several mordant, and three repeated dying at $50^{\circ}C$ for 1.5hr. 3. The wash fastness of the dyed wool fabric when it is washed with neutral detergent is more effective than alkaline detergent. The dry cleaning fastness of the dyed wool fabric is more excellent. In addition, the perspiration fastness of the dyed wool fabric is increased by mordanting method. And than the rubbing fastness of the dyed wool fabric is showed excellent under dryness and wetness. Light fastness of the dyed wool fabric, however is showed inferiority. 4. The wool fabric dyed with Opuntia ficus-indica extract is showed effective bacterial reduction and UV-B protection is increased remarkably.

• 한국식품조리과학회지
• /
• 제18권2호
• /
• pp.200-205
• /
• 2002

본 연구는 옥수수 전분의 기능 특성을 개선시키고자, 다양한 sodium hypochlorite(NaOCl)농도에서 산화시킨 산화 전분의 분자 구조적 변화에 대하여 시험하였다. 전분의 분자량 분포에서 산화 전분은 고분자량의 피크가 점차 작아지고 저분자량 부분의 피크가 높아져 전체적 분자량 분포가 낮은 쪽으로 이동하였고, 요오드 최대 흡수 파장이 감소되면서 색깔이 변화하였다. 무처리 전분은 온도가 증가함에 따라 분자량이 큰 부분의 용출이 증가하였다. 산화전분의 경우 아밀로펙틴이 산화에 의해 분해되어 전분 입자내에 큰 분자량을 가진 분자가 감소되면서 작은 분자량을 가지는 분자가 증가되기 때문에 가용성 탄수화물의 양이 증가하였다. DSC 특성에서는 상전이 온도 범위와 엔탈피의 차이는 없었으나 산화 전분이 무처리 전분보다 노화경향이 더 크게 나타났다. 산화전분용액의 광투과도는 증가하였다.

• 한국식품과학회지
• /
• 제22권7호
• /
• pp.732-737
• /
• 1990

HPLC를 이용하여 메밀종자 및 메밀식품에서의 rutin 함량을 분석하였다. 메밀종자의 rutin 함량은 재래종과 신농1호 사이에 상당한 차이가 있었으나. 신농1호 품종간 즉, 여름형과 가을형간에는 차이가 없었다. 가식부분인 메밀쌀의 rutin 함량은 여름형 신농1호에서 가장 높았다. 건조메밀국수와 막국수의 rutin 함량은 시료에 따른 차이가 상당히 컸었으며, 이와 같은 차이는 메밀국수 제조시 밀가루를 $10{\sim}50%$ 혼합하는데 기인하는 것으로 생각되었다. 메밀전병은 제품의 표시에 명기된 메밀함량으로부터 계산된 값보다 높은 rutin 함량을 나타내었다.

• 한국통신학회논문지
• /
• 제30권10C호
• /
• pp.1007-1016
• /
• 2005

본 논문은 3차원 모델의 모양 기반 검색을 하기 위한 모델의 특징을 추출하는 방법을 제시한다. 3차원 모델의 특징 기술자는 모델에 대한 위치, 회전, 크기 변환에서 그 특징이 불변해야 하기 때문에, 모델을 정규 좌표계로 표시하기 위한 선(先)처리 작업이 필요하다. 우리는 선처리 작업을 위해서 주성분 분석 방법을 사용하였으며, 이 방법은 최소 경계 상자와 외접구의 생성을 위해서도 이용되었다. 제안한 알고리즘은 다음과 같다. 반지름 1인 외접구를 만들고, 구의 중심에 3차원 모델을 위치시킨 후, 반지름이 다른 동심구($r_i=i/n,\;i=1,2,{\ldots},n$)를 생성하고, 이 동심구들과 모델이 접하는 면을 구한 다음 그 면에 대한 곡률을 계산한다. 여기서 구한 곡률을 3차원 모델의 특징 기술자로 사용하게 된다. 실험 결과는 타 알고리즘에 비해 제안하는 방법이 상대적으로 적은 빈(bin) 수를 가졌음에도 불구하고 ANMRR 평가 함수에 의해 최소 0.1에서 0.6 이상의 성능 개선 효과가 나타나고 있음을 보여 준다. 본 논문은 색인 기법으로 $R{^*}-tree$를 사용하였다.