• Title/Summary/Keyword: $C_{}$ max/

Search Result 1,801, Processing Time 0.026 seconds

Bacillus licheniformis KFB-C14가 생산하는 내열성 Chitinase의 정제 및 특성

  • Hong, Bum-Shik;Yoon, Ho-Geun;Shin, Dong-Hoon;Cho, Hong-Yon
    • Microbiology and Biotechnology Letters
    • /
    • v.24 no.5
    • /
    • pp.567-573
    • /
    • 1996
  • Chitinase (EC 3.2.1.14) from culture fluid of Bacillus licheniformis KFB-C14 was purified 66-folds to homogenity in overall yield of 21% by ammonium sulfate fractionation, DEAE-Toyopearl, Butyl-Toyopearl and TSK-Gel HW-55F column chromatography. The enzyme protein had a molecular weight of about 86,000 and was composed of one subunit. The enzyme was significantly stable not only at high temperature but also on treatment with organic solvents and protein denaturants such as SDS, urea and guanidine-HC1. The optimum temperature and pH for reaction was 60$\circ $C and 6.0, respectively. The enzyme activity was inhibited by only Mn$^{2+}$ ion, but not inhibited by EDTA, N- ethylmaleimide and pCMB. The enzyme had high activity with colloidal chitin (V$_{max}$: 421) and commercial chitin (V$_{max}$: 480), but not with typical substrates of exo type chitinase. The thermostable chitinase had an useful reactivity for producing functional chitooligosaccharide, showing the production of (GlcNAc)$_{1}, (GlcNAc)$_{3}$, and (GlcNAc)$_{2}$ as major product.

  • PDF

A Study on the Governing Factor of Fatigue Limit in Austempered Ductile Iron (오스템퍼링 구상흑연주철의 피로한도 지배인자에 관한 연구)

  • 정회원;김진학
    • Transactions of the Korean Society of Automotive Engineers
    • /
    • v.7 no.5
    • /
    • pp.221-229
    • /
    • 1999
  • We examined the governing factors of fatigue limit in annealed and austempered ductile iron specimens machined micro hole(dia.<0.4mm) in rotary bending fatigue test. Also, the quantitative relationship between fatigue limit and maximum defect size in specimens was investigated. Artificial defect(micro-pit type, dia.<0.4mm) on specimen surface did not bring about an obvious reduction of fatigue limit in austempered ductile iton(ADI) as compared with annealed ductile iron. According to the investigation of ${\sqrt{area}}_c$ which is the critical defect size to crack initiation at artificial defect, ${\sqrt{area}}_c$ of ADI was larger than that of annealed ductile iron. This shows that the situation of crack initiation at artificial defect in ADI is more difficult in comparison with annealed ductile iron. Maximum defect size is one of the important parameters to predict fatigue limit. And, the quantitative relationship, between the fatigue limit ${\sigma}_{\omega}$ and the maximum defect size ${\sqrt{area}}_{max}$ can be expressed to ${\sigma}_{\omega}^n{\cdot}{\sqrt{area}}_{max}=C_2$ where, $C_2$ are constant. Moreover, it is possible to explain the difference in fatigue limit between, austempered and annealed ductile iron by introducing the parameter ${\delta}(=N_{sg}/N_{total})$in a plain spectimen.

  • PDF

Magnetic Properties of Sintered Fe Cores Containing Stainless Steel-making Dusts (스테인레스 제강 분진을 함유한 순철 소결코아의 자기특성)

  • 김상원
    • Journal of the Korean Magnetics Society
    • /
    • v.11 no.3
    • /
    • pp.114-121
    • /
    • 2001
  • Effect of sintering temperature and dusts content on sintered density ($\rho$) and magnetic properties were evaluated. Cores sintered at 1350 $^{\circ}C$ for 1 hour with the Fe powders containing up to 10 wt.% dusts showed a good AC/DC properties. For example, the DC magnetic properties of magnetic induction (B$_{15}$), coercive force (H$_{c}$) and permeability ($\mu$$_{max}$) of cores containing 10 wt.% dusts were ranging over 9.8~ 10.9 kG, 2.2-2.3 Oe and 1950~2050, respectively. These magnetic properties are equivalent to those of competitor's (i.e, Ancorsteel produced by Hoganas). The cores obtained from the present work are expected to apply for high-performance soft magnetic components such as automotive and DC motor.

  • PDF

THE EFFECT OF ENVIRONMENT ON THE PHYSICAL PROPERTIES OF CORE MATERIALS (지대치 재료의 환경변화에 따른 물리적 성질에 관한 연구)

  • Hwang, Yoo-Sook;Choi, Kyoung-Kyu;Park, Sang-Jin
    • Restorative Dentistry and Endodontics
    • /
    • v.30 no.2
    • /
    • pp.86-94
    • /
    • 2005
  • The purpose of this study was to measure the flexural strength and hardness of four core materials in 4 different medias and to evaluate the relationship between the physical properties. For the flexural strength, the specimens were prepared from each of the following materials: Bisfil Core, Core Max, Fuji IX GP, Miracle Mix and randomly divided into four groups and stored at 37 degree C in the following medias: distilled water for 24 hours (DW/1), distilled water for 30 days (DW/30). $2%$ NaF for 30 days (NF/30), 0.02N lactic acid for 30 days (LA/30). After storage, the specimens were subjected to flexural strength testing and calculated to flexural modulus. For hardness testing, specimens were prepared from four materials and storaged in the uniform way. After storage, the specimens were subjected to Vicker's hardness testing. 1. The flexural strength of Core Max were the highest, and the flexural strength of Miracle Mix were the lowest. 2. The hardness of Bisfil Core were the highest. 3. The hardness of Core Max were the highest. 4. The hardness of Miracle Mix were the lowest. 5. $2\%$ NaF and 0.02N lactic acid negatively affected the flexural strength and hardness of four core materials.

The Properties of Proteolytic Enzymes in Fruits (Pear, Kiwifruit, Fig, Pineapple and Papaya) (배, 키위, 무화과, 파인애플, 파파야에 존재하는 단백질 분해효소의 특성 비교)

  • 배영희;노정해
    • Korean journal of food and cookery science
    • /
    • v.16 no.4
    • /
    • pp.363-366
    • /
    • 2000
  • This study was attempted to compare the properties of proteolytic enzymes in fruits(Pear, Kiwifruit, Fig, Pineapple and Papaya) for the application of scientific information to cooking. The results were as follows: 1. The optimum temperature of crude proteolytic enzymes in pear, fig and pineapple is 60$^{\circ}C$ and it was relatively active in 40-70$^{\circ}C$; papaya showed max. activity in 60$^{\circ}C$ and highly stable activity in 40-80$^{\circ}C$, but kiwifruit showed max. activity in 40$^{\circ}C$ and it maintained to 70$^{\circ}C$. 2.. The crude proteolytic enzymes of pear, fig, pineapple and papaya showed opt. pH at pH 7.0 and maintained at pH 5.0-8.0, but max. activity of kiwifruit observed in pH 3.0 and pH 5.0-8.0. 3. As a result of comparison of total activities of fruits per kg unit, the order of activities was pineapple > kiwifruit > papaya > fig > pear.

  • PDF

Classification of Seismic Stations Based on the Simultaneous Inversion Result of the Ground-motion Model Parameters (지진동모델 파라미터 동시역산을 이용한 지진관측소 분류)

  • Yun, Kwan-Hee;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
    • /
    • v.10 no.3
    • /
    • pp.183-190
    • /
    • 2007
  • The site effects of seismic stations were evaluated by conducting a simultaneous inversion of the stochastic point-source ground-motion model (STGM model; Boore, 2003) parameters based on the accumulated dataset of horizontal shear-wave Fourier spectra. A model parameter $K_0$ and frequency-dependent site amplification function A(f) were used to express the site effects. Once after a H/V ratio of the Fourier spectra was used as an initial estimate of A(f) for the inversion, the final A(f) which is considered to be the result of combined effect of the crustal amplification and loca lsite effects was calculated by averaging the log residuals at the site from the inversion and adding the mean log residual to the H/V ratio. The seismic stations were classified into five classes according to $logA_{1-10}^{max}$(f), the maximum level of the site amplification function in the range of 1 Hz < f < 10 Hz, i.e., A: $logA_{1-10}^{max}$(f) < 0.2, B: 0.2 $\leq$ $logA_{1-10}^{max}$(f) < 0.4, C: 0.4 $\leq$ $logA_{1-10}^{max}$(f) < 0.6, D: 0.6 $\leq$ $logA_{1-10}^{max}$(f) < 0.8, E: 0.8 $\leq$ $logA_{1-10}^{max}$(f). Implication of the classified result was supported by observing a shift of the dominant frequency of average A(f) for each classified stations as the class changes. Change of site classes after moving seismic stations to a better site condition was successfully described by the result of the station classification. In addition, the observed PGA (Peak Ground Acceleration)-values for two recent moderate earthquakes were well classified according to the proposed station classes.

Using Tabu Search for L(2,1)-coloring Problem of Graphs with Diameter 2 (Tabu Search를 이용한 지름이 2인 그래프에 대한 L(2,1)-coloring 문제 해결)

  • Kim, SoJeong;Kim, ChanSoo;Han, KeunHee
    • Journal of Digital Convergence
    • /
    • v.20 no.2
    • /
    • pp.345-351
    • /
    • 2022
  • For simple undirected graph G=(V,E), L(2,1)-coloring of G is a nonnegative real-valued function f : V → [0,1,…,k] such that whenever vertices x and y are adjacent in G then |f(x)-f(y)|≥ 2 and whenever the distance between x and y is 2, |f(x)-f(y)|≥ 1. For a given L(2,1)-coloring c of graph G, the c-span is λ(c) = max{|c(v)-c(v)||u,v∈V}. L(2,1)-coloring number λ(G) = min{λ(c)} where the minimum is taken over all L(2,1)-coloring c of graph G. In this paper, based on Harary's Theorem, we use Tabu Search to figure out the existence of Hamiltonian Path in a complementary graph and confirmed that if λ(G) is equal to n(=|V|).

PERIODIC AND CORRELATION ANALYSES BETWEEN WATER TEMPERATURE AND AIR TEMPERATURE IN THE KOREAN WATERS (韓國 沿岸 水溫 및 氣溫의 週期分析과 相關分析)

  • Kim, Bok-Kee
    • 한국해양학회지
    • /
    • v.18 no.1
    • /
    • pp.55-63
    • /
    • 1983
  • The study on the periodic and correlation analysis between water temperature and air temperature has beenconducted by oceanographic data obtained from 1923 to 1979 (For 16-51 years) in 6 ststions in the Korean Waters. The periodic and correlation analyses has been examined by method of he Schuster's and the quadratic formula of least squares method, respectively. The results pbtained from the study are as follows; 1. Periodic analysis 1) The yearly difference between max. and mini. fo surface water temperature was 12.77-17.99$^{\circ}C$ (computed value : 11.67-16.64$^{\circ}C$) in offshore waters, and was 15.72-26.33$^{\circ}C$ (computed value : 15.13-25.29$^{\circ}C$) in inshore waters, and that of air temperature was 21.71-28.60$^{\circ}C$ (computed value : 10.50-27.22$^{\circ}C$). 2) The yearly mean of water temperature by station was 11.25-18.78$^{\circ}C$, and that of air temperature was 11.39-16.16$^{\circ}C$. 3) The annual compnent amplitrde of water temperature was 5.72-12.54$^{\circ}C$, and that of air temperature was 10.04-13.49$^{\circ}C$. 4) The semi-annual component amplitude of water temperature was 0.83-1.30$^{\circ}C$, and that of air temperature was 0.72-1.26$^{\circ}C$. 5) The annual component phase of water temperature was 215-228$^{\circ}C$ (max. temperature shall be in the first and in the middle ten days of August) in inshore waters and 138-244$^{\circ}C$ (max. temperature shall be in the first and in the middle ten days of August) in offshore waters, and that of air temperarture was 212-221$^{\circ}C$ (max. temperature shall be in the first and in the middle ten days of July and in the first tin days of August). 6) The semi-annual component phase of water temperature was 87-110$^{\circ}C$ in offshore waters, and 167-212$^{\circ}C$ in inshore waters, and that of air temperature was 156-189$^{\circ}C$. 2. Correlation analyses of water temperature and air temperature before one month. 1) When the water temperature is in rising time, the quadratic constant of correlation formual was the gradual inreasing type ( constant; 0.010-0.026) in offshore waters, and the gradual decreasing or proportional type (constant; -0.020-0.001) in inshore waters. 2) when the water temperature is in descending time, the quadratic constant of correlation formula was the gradual increasing type (constant: 0.012-0.021) 3) the determination coefficient was 0.964-0.992 at rising time and 0.982-0.999 at descending time of water temperature.

  • PDF