• 한국약용작물학회지
• /
• 제17권1호
• /
• pp.1-7
• /
• 2009

Several parts of Lythrum salicaria were used for this study. Scavenging activities on radicals, inhibitory activity on linoleic acid peroxidation and total phenol contents of extracts from root, flower, and aerial part were evaluated. Flower and root selected from in vitro assay were subjected to in vivo assay on $CCL_4$-induced liver injury rat model for two weeks. Carbon tetrachloride intoxication on rats produced large amounts of hepatic lipid peroxidation product, thiobarbituric acid reactive substance (TBARS) compared with normal rats. Treatment with root extract of L. salicaria (LSR) showed effective inhibitory activity on lipid peroxidation product. Administration with LSR extract significantly alleviated $CCL_4$-induced increase in GPT activity which were more effective than silymarin. The results of this study suggest that root and flower of L. salicaria have antioxidant and liver protecting activities, and root part is the most effective candidate to develop a new functional material.

• 한국식품과학회지
• /
• 제34권4호
• /
• pp.684-687
• /
• 2002

귀뚜라미의 곤충산업의 발달로 대량체계화 사육된 귀뚜라미의 새로운 용도 개발의 일환으로 귀뚜라미의 간보호 효과를 조사하였다. 사염화탄소로 6주령의 ICR 응성 생쥐에 급성간염을 유발시킨 후 귀뚜라미 물 추출물(20 mg/kg) 및 메탄올 추출물(20 mg/kg)을 각각 투여하여 GOT, GPT, LDH 간혈청 효소 활성을 조사한 결과 귀뚜라미 메탄올 추출물은 대조구에 비해 GOT, GPT, LDH치가 74, 50, 101% 감소하였다. 지질과산화 억제정도는 귀뚜라미 물추출물 및 메탄올추출물이 정상군 대비 40%이상의 억제 효과를 나타내었다. 또한 강제수영법에 의한 귀뚜라미 추출물의 항피로 효과를 측정시 대조군에 비해 메탄올 200 mg/kg에서 현저한 수영시간 증가를 보여 양성대조군인 토코페롤 100 mg/kg 수준의 유의성 있는 항피로 효과를 나타낸 것을 알 수 있었다. 이상의 결과로 보아 귀뚜라미의 수용성 및 알콜추출물은 간보호 효과를 가지는 건강보조식품으로서 개발 가능성을 가짐을 알 수 있다.

• 생약학회지
• /
• 제31권1호
• /
• pp.45-50
• /
• 2000

The root of Pseudostellaria heterophylla Pax et Hoffm. (Caryophyllaceae) is a herbal medicine called Taejasam(太子蔘) and used as a good tonic in China that strengthens the functions of the lung and the stomach like Ginseng in Korea. It has been known to have the antitussive activity for tuberculosis, appetizing effect and antifatigue activity, and so on. Especially it has been known to generate body fluids for the severe thirst and the shortage of water in body after a febrile disease for a long time. Recently some components of cyclic peptides, pseudostellarins A,B,C,D,E,F,G, that inhibit the activity of tyrosinase, were separated from this root. In this report several biological activities including acute toxicity, analgesic activity, antifatigue activity, bile juice increasing activity, hepatoprotective activity and antiinflammatory activity were investigated. These results showed as such; $LD_{50}$ of methanol extracts was above 10,000 mg/kg. The analgesic effect was revealed in the writhing test using rats. The BuOH fractions showed a prominent antifatigue effect against immobilized stress, significant hepatoprotective activities against $CCl_4$ intoxication and inhibitory effect on carrageenin-induced edema in rat's paw.

• 한국약용작물학회지
• /
• 제18권5호
• /
• pp.323-328
• /
• 2010

Root extract of Lythrum salicaria reported a hepato-protective effect on $CCl_4$-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), n-butanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and $CuSO_4$-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at $50\;{\mu}g/m{\ell}$) on linoleic acid autoxidation at $40^{\circ}C$, which was more effective than $\alpha$-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 mg/kg/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride ($1\;m{\ell}/kg$ in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-$\alpha$), a proinflammatory cytokine, elevated with $CCl_4$-intoxication in negative control group ($83\;pg/m{\ell}$) was significantly decreased in E fraction-supplemented group ($18\;pg/m{\ell}$). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/mg protein) was decreased in E fraction (0.07 U/mg protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.

• 생약학회지
• /
• 제16권2호
• /
• pp.81-92
• /
• 1985

The Polygoni Radix and Cynanchi Radix have been used to potentiate the liver functions in clinic of Oriental Medicine. The water extracts of Polygoni Radix and Cynanchi Radix were administered orally to rats intoxicated with carbon tetrachloride and then this experiment have been performed by observing liver fatty degeneration and activities of enzymes such as cytochrome oxidase (CYO), adenosine triphosphatase (ATP), acid phosphatase (ACP), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP). By oral administration of water extracts of the radices between 1 and 10 days, the following results were obtained. 1. The group given Cynanchi Radix extract showed recovery of the fat liver in 4 days, whereas that given Polygoni Radix extract did the recovery in 8 days. 2. In cytochrome oxidase activity, the group given Cynanchi extract showed normal activity in 6 days, whereas that given Polygoni Radix extract did the activity in 8 days. 3. In adenosine triphosphatase activity, the groups given Cynanchi Radix and Polygoni Radix extracts showed normal activities in 2 and 8 days, respectively. 4. In acid phosphatase activity, the groups given Cynanchi Radix and Polygoni Radix extracts showed recovery of the activities in 2 and 4 days, respectively. 5. In lactate dehydrogenase activity, the group given Cynanchi Radix and Polygoni Radix extracts showed recovery of the activities in 6 and 10 days, respectively. 6. In alkaline phosphatase activity, the group given Cynanchi Radix extract showed normal activity in 2 days, whereas that given Polygoni Radix extract showed slight recovery between 4 and 6 days followed by decrease of the activity in 8 and 10 days. From the above-mentioned results, it was found that both of the water extracts of Polygoni and Cynanchi Radix possessed the recovery action of liver function as intoxicated with carbon tetrachloride in rats. It is also noted that the extract of Cynanchi Radix showed more potent activity than that of Polygoni Radix.