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채식인과 비채식인의 섭취열량, 소비열량 및 활동량과 심혈관질환 관련인자와의 관련성에 관한 비교연구 (A Comparative Study of Relationships among Energy Intakes, Energy Expenditure, Physical Activity and Cardiovascular Disease Related Factors in Vegetarians and Non-Vegetarians)

  • 차복경
    • 한국식품영양과학회지
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    • 제30권2호
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    • pp.350-356
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    • 2001
  • 우리나라에도 심혈관질환으로 인한 사망률이 점차 증가하고 있다. 이에 본 연구에서는 채식과 심혈관질환과의 관계를 규명하기 위한 연구의 일환으로 채식을 하는 비구니스님을 대상으로 하여 연구한 결과를 요약하면 다음과 같다. 조사 대상자의 평균나이는 채식인 44.20세, 비채식인 40.52세, BMI는 각각 22.47, 21.08이었고, WHR은 0.85, 0.84였고, %BF는 28.79, 26.55였으며, 채식인의 평균채식기간은 13.16년이었다. 조사 대상자의 영양소 섭취실태는 채식인은 모든 영양소가 권장량을 상회하였고, 비채식인은 Ca, 비타민 A가 권장량을 미달하였다. 섬유소와 비타민 C 섭취량은 채식인이 유의적으로(p<0.01) 높았다. 조사 대상자의 소비에너지는 채식군 1789.84 kcal, 비채식군 1703.83 kcal로 채식군이 유의하게 많았고(p<0.05), 활동량도 채식군 507.76 kcal, 비채식군 400.03 kcal로 채식군이 유의하게 높았다(p<0.01). 조사 대상자의 total cholesterol, LDL-cholesterol, AI, 이완기혈압 및 혈당은 비채식인이 유의적으로(p<0.01) 높았고, HDL-cholesterol은 (p<0.05) 비채식인이 유의적으로 높았으며 심질환 예견지수인 HDL/total cholesterol 비는 채식인이 유의적으로(p<0.01) 높았다. 활동량은 두군 모두 중성지방, 혈청 총 콜레스테롤, LDL-cholesterol, AI와는 역상관을 보였다(p<0.05). 이상의 결과에서 채식군은 비채식군에 비해 혈중지질 수준, 혈당, 혈압에 영향을 미치는 것으로 알려진 BMI, RBW, WHR,%BF가 유의적으로 높았음(p<0.05)에도 불구하고 혈정 총콜레스테롤, LDL-choleserol, AI 및 수축기혈압이 유의적으로 낮았는데 이는 여러가지 요인이 있겠지만 채식군이 비채식군에 비해 섬유소, 비타민 C의 섭취량 및 활동량이 유의하게(p<0.01) 높았기 때문으로 생각된다. 따라서 혈중지질 수준, 혈당, 혈압에 영향을 미치는 것은 식이요인 뿐만 아니라 활동량 등 여러가지 생활양식이 영향을 미치는 것으로 볼 수 있다.

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초음파측정 활용 고급육형 한우개량을 위한 선발반응 Monte Carlo 모의실험 (Monte Carlo Simulations of Selection Responses for Improving High Meat Qualities Using Real Time Ultrasound in Korean Cattle)

  • 이득환
    • Journal of Animal Science and Technology
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    • 제45권3호
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    • pp.343-354
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    • 2003
  • 고급육형 한우개량을 위하여 현행 실시하고 있는 한우 후대검정에서 조사되는 배장근단면적, 등지방두께 및 근내지방도 등에 대한 유전적 개량량과 초음파 측정장치를 이용한 해당 형질들의 육질판정자료에 의한 간접선발시 유전적 개량량을 모의실험을 통하여 추정하고 상호 그 효율성을 비교하였다. 본 연구에 적용된 Monte Carlo 모의실험은 각 설정 모형별, 표현형 선발 및 다형질 BLUP 추정방법에 의한 육종가 추정치에 의한 선발을 적용하여 각각 10반복씩 반복 실험하였다. 각 모형별 모의실험방법은 종축집단의 크기 및 세대 수 그리고 설정모형을 동일하게 설계하였으며 수컷 100두와 암컷 2,000두의 기초집단으로부터 매 세대 수컷 20두와 암컷 1,000두를 3가지 형질에 동일한 가중치를 두어 10세대 동안 선발하였다. 최종 생성된 자료를 이용하여 유전적 개량량을 추정하였고 이를 각 모형별, 선발방법별로 비교, 분석하였다. 분석결과 현행 실시하고 있는 한우 후대검정과 유사한 모의실험에서의 각 형질별 육종가 추정치에 의한 선발시 유전적 개량량과 비교한 결과, 초음파 측정치와 도축 실측치 간의 유전상관을 0.81-0.97으로 가정하고 암, 수 모두 초음파 측정자료를 조사한다는 가정으로 초음파 측정치의 육종가에 의한 선발을 실시할 때, 1.66${\sim}$2.44 배의 개량 효율성이 있는 것으로 추정되었으며 이들 상관을 0.63${\sim}$0.68으로 가정한 모의실험에서는 1.18${\sim}$2.08 배의 개량 효율성이 있는 것으로 추정되었다. 따라서 고급육형 한우개량을 위한 초음파 측정치를 이용한 검정방법을 활용하고 이를 이용한 종축선발을 실시할 때 개량효율을 크게 향상시킬 수 있을 것으로 사료되었다. 반면에 각 형질별 개량효율성을 살펴보면 개량효율성이 가장 낮은 형질은 근내지방도로써 이는 표현형 변이가 상대적으로 낮은 형질에 선발의 가중치를 동일하게 가정한 것에 기인된 것으로 사료되며 따라서 근내지방도의 변이를 크게 할 수 있는 자료조사방법의 변경, 또는 선발의 가중치를 높이는 방법에 대한 연구가 필요할 것으로 사료되었다.

한우 FASN 유전자변이와 한우 지방형질 및 지방산조성과의 연관성 분석 (Association of genetic polymorphism in fatty acid synthase with BodyFat and fatty acid composition in Hanwoo)

  • 이승환;김형철;조원모;장선식;김범수;장길원;이준헌;연성흠;홍성구
    • 농업과학연구
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    • 제38권2호
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    • pp.257-262
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    • 2011
  • The aim of this study was to identify the polymorphism on fatty acid synthase (FASN) gene and its association with fat traits and fatty acid composition in Hanwoo. We genotyped a SNP (g.16024G${\rightarrow}$A) detected in Exon34 of FASN on 90 Hanwoo steers by PCR RFLP. A linear mixed model revealed an association of g.16024G${\rightarrow}$A with total body fat contents (P=0.006), while there is no significant effect between g.16024G${\rightarrow}$A and other traits (IMF, BF and fatty acid composition). Regression coefficient and $R^2$ of the SNP was -1.5 kg and 0.36 in this anslysis. Expecially, AA type of g.16024G${\rightarrow}$A bas a less amount of body fat (1.5 kg) than GG type of the SNP in Hanwoo. In conclusion, this study indicates an important role for FASN gene in determining body fat content in Hanwoo.

Effects of substitution of viral hemorrhagic septicemia virus genotype IVa glycoprotein with vesicular stomatitis virus (VSV) glycoprotein on cell line preference

  • Kim, Min Sun;Choi, Tae-Jin;Kim, Ki Hong
    • 한국어병학회지
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    • 제30권2호
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    • pp.71-78
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    • 2017
  • The glycoprotein of novirhabdoviruses is known to play a critical role in the determination of host specificity. Viral hemorrhagic septicemia viruses (VHSVs) in different genotypes have different glycoprotein sequences and show different preferences for specific cell lines. In this study, to know whether the glycoprotein is solely responsible for the host cell preference of VHSV, a recombinant VHSV expressing vesicular stomatitis virus (VSV) glycoprotein instead of VHSV IVa glycoprotein (rVHSV-VSV-G) was generated by reverse genetics and inoculated into several fish cell lines, then, cytopathic effect (CPE) and viral growth caused by rVHSV-VSV-G infection were compared with those caused by rVHSV-wild that was previously generated and has the same genomic sequence with wild-type VHSV except a few nucleotides. The plaque numbers of rVHSV-VSV-G were significantly higher in EPC, BF-2 and GF cells than those of rVHSV-wild. However, in HINAE cells (originated from olive flounder), rVHSV-VSV-G titer was significantly lower than rVHSV-wild titer, and both recombinant VHSVs were not grown well in CHSE-214 cells. Although statistical significances were detected in the titers between rVHSV-wild and rVHSV-VSV-G in several cell lines, the cell line-preference order of rVHSV-VSV-G was not different from that of rVHSV-wild. These results suggest that the replacement of VHSV glycoprotein may not completely change host cell preference, and other regions of VHSV might also involve in the determination of host cell preference.

Electrochemical Properties of Buckminsterfullerene ($C_{60}$) in Acetonitrile Containing Quarternary Ammonium Electrolytes

  • Kim, Il Kwang;Kim, Hyun Jin;Oh, Gi Su;Jeon, Il Chol;Ahn, Byoung Joon
    • 분석과학
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    • 제8권4호
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    • pp.675-682
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    • 1995
  • Thin films of buckminsterfullerene($C_{60}$) formed by solution drop casting on Pt foil electrode surfaces were studied by cyclic voltammetry(CV) in acetonitrile(MeCN) containing quaternary ammonium or alkali-metal salts as supporting electrolyte. The electrochemical behaviors of $C_{60}$ films are found to be strongly dependent on the nature of the supporting electrolytes, especially with tetrabutyl ammonium perchlorate (TBAP, $NBu_4ClO_4$), and tetrabutyl ammonium tetrafluoroborate ($TBABF_4$, $NBu_4BF_4$). Reasonably stable films are formed into which electrons can be injected. The interaction of $C_{60}$ film with the quaternary ammonium cation may produce the fulleride salts $(TBA^+)(C{_{60}}^-)$ and $(TBA^+)_2(C{_{60}}^{2-})$. The bulk electroreduction with a controlled potential to generate the soluble $C{_{60}}^{3-}$ anions(dark red-brown color) is followed by electrooxidative deposition to produce a neutral $C_{60}$ film on the surface. The peak currents($I_{pc}$ and $I_{pa}$) of these thin film were dramatically decreased with repetitive potential scanning. These results could be explained by the adsorption-desorption phenomena and ion pairing interaction of reduced species($C{_{60}}^-$, and $C{_{60}}^{2-}$) onto the electrode surface. The peak current changes and peak potential shifts of the thin $C_{60}$ film in cyclic voltammograms formed from solution were observed by varying scan rates.

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모유영양아와 인공영양아의 칼슘과 인의 섭취상태에 관한 종단적 연구 (A Longitudinal Study of Calcium and Phosphorus Intakes of Korean Infants from 1 to 3 Months in Breast-Fed vs Formula-Fed Infants)

  • 구재옥;최경숙
    • 대한지역사회영양학회지
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    • 제5권2호
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    • pp.273-279
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    • 2000
  • This study was carried out to evaluate the calcium and phosphorus intakes and correlation with growth performance of 25male breast and formula-fed infants from 1 to 3 months postpartum. There were four groups breast-fed(BF) and three formula-fed groups(FFM, FFN and FFP). There was no significant difference in the height and weight of infants according to feeding methods and formula brands. Milk intake and the concentration of calcium and phosphorus from human milk and the formulas were measured. The average calcium content of human milk was 26.6$\pm$4.1mg/dl and 13.5$\pm$2.0mg/dl, respectively. The average calcium intakes of the brest-fed and formula-fed infants were 205.5$\pm$29.3mg/day and the 460.5$\pm$70.6mg/day, respectively the average calcium intake of breast-fed infants was significantly lower than that of formula fed infant. The percent of RDA(41%) of breast-fed infants was lower than that(75.4%) of formula fed infant. The average phosphorus intake of the brest-fed and formula-fed infants were 105.1$\pm$20.1mg/day and 288.3$\pm$37.3mg/day, respectively. The precent of RDA(27%) for brest fed was significantly lower than that(75.4%) of formula fed. The Ca/P ratios were 2.1 in brest fed and 1.6 in formula fed infant. The average calcium and phosphorus intakes of the formula fed infants was higher than that of the brest-fed infants. This data suggests the calcium and phosphorus intakes form human milk sufficiently support the growth of infants. Therefore, the level of calcium and phosphorus recommended dietary allowances for infants should be reduced.

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실내·외 보행환경의 변화가 40대와 노인의 보행속도와 다리 근활성도에 미치는 영향 (Effects of Changes in the Indoor and Outdoor Environmental on the Walking Speed and Lower Extremity Muscle Activities in People Aged Forty and Older than Seventy Years)

  • 이준영;김택훈
    • 대한물리의학회지
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    • 제13권4호
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    • pp.139-148
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    • 2018
  • PURPOSE: This study compared the effects of indoor and outdoor environmental changes on the activity of the major lower limb muscles and walking factors in people 40-50 years and those aged older than 70 years. METHODS: Ten middle-aged people in their forties (age:$44.2{\pm}2.7$, BMI:$21.8{\pm}1.8$) and 10 elderly aged more than 70 years (age:$76.4{\pm}5.9$, BMI:$22.2{\pm}1.9$) with a normal walking ability were included. The participants walked 100 m both indoors and outdoors at their own speed. Using a 3D motion analyzer and EMG, the walking speed, angle of the ankle and activity changes of the lower limb muscles were compared. RESULTS: Significant differences in walking speed and peak-plantar flexion angle were observed between the two groups (p<.05). The muscular activity of the gastrocnemius muscle (GCM) was significantly different outdoors in the swing phase between the two groups (p<.05). In the people aged in their forties, the muscular activity of the rectus femoris (RF) was significantly higher outdoors than indoors (p<.05). In the elderly, however, the muscular activity of the RF was lower outdoors than indoors (p<.05). When compared to those in there forties, the muscular activity of the outdoor RF significantly decreased in the elderly group (P<.05). The muscular activity of the biceps femoris (BF) in the elderly decreased significantly outdoors compared to indoors (p<.05). CONCLUSION: For the elderly, increasing the exposure to the new environments or focusing on the performance of repeated movements for gradual speed control and precise movements is required to maintain normal gaits and movements that are less affected by environmental changes.

무릎외반의 균형 검사 및 보행 중에 근활성도와 발바닥압의 평가 (Evaluation of Muscle Activity and Foot Pressure during Gait, and Balance Test in Patients with Genu Valgum)

  • 윤정욱;유경태;이호성
    • 대한물리의학회지
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    • 제17권1호
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    • pp.127-137
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    • 2022
  • PURPOSE: This study sought to evaluate muscle activity and foot pressure during gait, and balance in female college students with genu valgum. METHOD: Participants were assigned based on their Q-angle to genu valgum group greater than 20° (GVG, n = 12), unilateral genu valgum group greater than 20° (UVG, n = 11), and control group (CON, n = 13). All subjects were evaluated for balance (Trace length, C90 area, C90 angle, and the Romberg test), muscle activity (gluteus medius; GM, tensor fasciae latae; TFL, vastus medialis; VM, vastus lateralis; VL, biceps femoris; BF, gastrocnemius; GCM and tibialis anterior; TA) and foot pressure (F/F ratio, R/F ratio, Hallux, 2~5 toe, 1st MT, 2~4 MT, 5th MT, Midfoot, M/heel, and L/heel) during gait. RESULTS: Romberg test showed significantly increased loss of balance in the UVG group compared with the CON. In the forward position, the imbalance was significantly increased in the UVG and GVG groups compared to the CON. Muscle activity of VL, GCM, and TA significantly increased in the GVG group compared with the CON. Static foot pressure, 1st MT significantly increased in the GVG compared to the CON group. The 5th MT significantly decreased in the CON compared with the GVG group. The R/F ratio significantly decreased in the GVG compared to the CON group. In dynamic foot pressure, the 2~5 toe significantly increased in the GVG compared with the UVG group. The left 5th MT significantly decreased in the UVG compared with the CON and GVG groups. CONCLUSION: These results indicate that genu valgum has a negative effect on balance, muscle activity, and foot pressure during gait in female college students.

남녀 대학생의 일반적 특성에 따른 모유수유 관련지식에 관한 연구 (A Study on Knowledge on Breast Feeding of College Students by Their General Characteristics)

  • 송병춘;조지현;이인열;김미경
    • 한국식생활문화학회지
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    • 제23권2호
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    • pp.268-272
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    • 2008
  • The breast feeding habits of 507 college students were evaluated between March 27 to April 26 (2006). Data were collected from self-administered questionnaires and analyzed using SPSS for Windows V.12.0. The study population consisted of 245 (48.3%) male and 262 (51.7%) female college students that answered ‘yes’ when asked whether they had been breast feed. A total of 52.7% of the study population were fed with breast milk (most frequent answer), as opposed to 11.0% that were fed with infant formula (least frequent answer) during their infancy. When asked whether they ever got educated on breast feeding, 78.1% of the college student subjects answered ‘no’. A total of 95.1% of the female college students replied ‘yes’ when asked if they intended to breast feed their child. Moreover, 62.2% of the college student subjects indicated that their parents had an influence on their decision to breast feed. A comparison between male and female college students indicated that female college students had a superior knowledge level of the general characteristics of breast feeding over the male subjects (p<0.05) . Moreover, a comparison of the different levels of college attained suggest that freshman college students had the highest knowledge level, followed by senior, sophomore and junior college students in terms of advantage, BF Tabu (p<0.05). Also, students provided with education on breast feeding had a higher knowledge level than college students with no formal education. In summary, the results suggest that the knowledge on breast feeding in college students were different by general characteristics such as gender, major and school year, and education on breast feeding in advance was appeared to be an important factor, therefore nutritional education course on breast feeding is recommended for the college students.

한국산 꽃소금과 천일염의 이화학적 특성 및 미생물 분석 (Physicochemical Properties and Microbial Analysis of Korean Solar Salt and Flower of Salt)

  • 이혜미;이우경;진중현;김인철
    • 한국식품영양과학회지
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    • 제42권7호
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    • pp.1115-1124
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    • 2013
  • 한국산 꽃소금의 특성을 알아보기 위하여 국내에서 생산된 꽃소금 3종과 천일염 1종의 이화학적 및 미생물 분석을 실시하였다. 수분함량은 $10.54{\pm}0.10{\sim}13.82{\pm}0.12%$, 나머지 조단백, 조지방, 조섬유는 거의 존재하지 않았다. NaCl은 신의도산 꽃소금이 $78.81{\pm}0.28%$, 비금산 꽃소금이 $81.67{\pm}0.34%$, 도초산 꽃소금 $84.61{\pm}0.21%$, 도초산 천일염이 $80.82{\pm}0.17%$로 나타났다. 불용분, 사분은 각각 0.01~0.05%, 0.01~0.03%로 낮게 검출되었다. 미네랄 분석은 도초산 꽃소금에서 K과 Mg 함량이 2,975.23 mg/kg, 9,886.72 mg/kg으로 비교적 낮게 나타났다. Ca은 도초산 소금 2종이 945.53 mg/kg, 942.43 mg/kg으로 낮은 함량을 보였다. 중금속 As, Cd, Pb, Hg은 모두 규격 이하로 검출되었다. 소금결정을 관찰한 결과로 천일염과 꽃소금 모두 핵이 중복되어 겹으로 적층된 것을 확인하였으며 크기는 비금산 꽃소금이 $0.067{\times}0.067mm$로 가장 작고 도초산 천일염이 $0.112{\times}0.124mm$로 꽃소금에 비해 크게 나타났다. 소금의 색도는 꽃소금의 L값이 천일염에 비해 높아 밝게 확인되었다. 관능검사 결과로 짠맛은 NaCl 함량과 유사하며, 쓴맛은 K과 Mg 함량이 적은 도초산 꽃소금이 낮게 나타났다. 천일염에 비해 꽃소금의 단맛과 기호도가 더 높은 것으로 확인되었다. 호염균 동정 결과 19종 모두 Firmicutes로 꽃소금에서 Marinibacillus, Paenibacillus, Bacillus 속이 12종으로 확인되며, 천일염은 Planococcus, Staphylococcus, Bacillus 속 3종으로 검출되었다. DGGE 실험 결과로 도초산 꽃소금에서 16개의 band와 도초산 천일염에서 15개의 band를 확인하였다. 동정 결과 도초산 꽃소금에서 Cupriavidus sp. ATHA3(14.43%), Cupriavidus sp. TSA5(10.41%), Maritimibacter sp. YCSD61-2(8.13%), uncultured bacteria(68%)로 확인되었고, 도초산 천일염에서는 Dunaliella salina(4.76%), Cupriavidus sp. ATHA3(15.80%), uncultured Mycoplasmataceae bacteria(51.72%), uncultured bacteria(27%)로 확인되었다.