• 미생물학회지
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• 제10권3호
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• pp.97-104
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• 1972

The extent of water pollution was investigated at 4 stations in Kyonggi Bay during the summer seasons in 1970 and 1971. The concentrations of dissolved oxygen, total hardness, ammonia, nitrate, nitrite, phosphate, chemical oxygen demand, salinity, biochemical oxygen demand, coliform bacteria and facel coli were examined together with the measurement of pH and transparency. The relationship between the extent of pollution and the distance from the Inchon Bay also was examined. The concentrations of biochemical oxygen demand, chemical oxygen demand, ammonia, nitrate, nitrite, phosphate, coliform bacteria nad fecal coli were all highest at station 1, and lowest at station 4. Values were somewhat higher at low tide level in general. On the contrary, dissolved oxygen concentration was highest at station 4 and lowest at station 1. The highest and lowest values of biochemical oxygen demand were 10.88 ppm at station 1 and 0.27 ppm at station 4. The chemical oxygen demand concentrations at station 1 and 4 were 1.90 ppm and 0.63 ppm. Ammonia concnetration at station 1 was 0.43 pp, and was nearly 5 times as much as that at station 4. The values were $2.45{\times}10^{-4}$ ppm at station 1, and $6{\times}10^{-4}$ ppm at station 4. Nitrite concnetration at station 1 was $3{\times}10^{-4}$ppm and was the highest, while the lowest was $9.45{\times}10^{-5}$ ppm at station 4. Phosphate value at station 1 too was the highest and was about 4 times as much as that at station 4. Coliform bacteria were most abundant at station 1, and were counted to be 1.$1.7{\times}10^{-5}$cells/ml. At station 4, the number greatly reduced to 8 * 10$^{2}$ cells/ml. The number of fecal coli at station 1 was $2{\times}10^{-4}$ cells/ml. But at station 4, no fecal coli was detected at high tide level. At low tide level, 3 cells/ml were counted at station 4. In all of these, the highest data were obtained at low tide level, while most of the lowest value, at high tide level. Generally, values at statio 1 were 3-5 times as much as those at station 4. Concentration of dissolved oxygen at station 1 was 0.366 mg-atoms/1 and was the lowest. The highest value was 0.420 mg-atoms/1 and was recorded at station 4. The highest at station 4, which certainly were believed to be the result of the dilution by the fresh water of the Han river flowing into the Inchon Bay. As we can see from the data above, the extent of pollution was highest at station 1, the nearest from Inchon harbor, and lowest at station 4, where is the farthest, Station 1 and 2 were proved to be much polluted, but station 3 and 4, not.

• 대한예방한의학회지
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• 제3권1호
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• pp.125-145
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• 1999

The effects of Wang-ttum, Magnetic Water, Magnetic field and Sibjeondaebotang on hemodynamics in sleep deprivation were studied. The results as follows; 1. In case of Wang-ttum operated group, significant changes were observed at 12 p.m., 2 a.m., 4 a.m. in maximum blood pressure for the first and second overnight stay and at 2 a.m. for the third and, respectively, average blood pressure at 12 p.m., 2 a.m. for the 1st and 2nd overnight stay, minimum blood pressure at 10 p.m.. 12 p.m.. 2 a.m. for the 1st overnight stay and at 10 p.m., 12 p.m. for the 2nd and at 12 p.m. for the 3rd, pulse rate at 12 p.m., 2 a.m., 4 a.m., 6 a.m., for 1st and 2nd and at 2 a.m., 4 a.m. for the 3rd and 4th, TP-KS at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and 2nd and at 2 a.m., 4 a.m., 6 a.m. for the 3rd, PRP at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and 2nd and at 12 p.m., 2 a.m., 4 a.m. for the 3rd and at 2 a.m., 4 a.m. for the 4th, TPR at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. from 1st to 4th overnight stay. 2. In case of taking magnetic water group, significant changes were observed at 2 a.m., 4 a.m. in pulse rate for the 1st overnight stay and, respectively, PRP at 2 a.m. for the 1st, TRP at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and 4th. 3. In case of attaching magnet group, TPR was significantly observed at 10 p.m. for the 1st overnight stay. 4. In case of medicating Sibjeondaebotang group, significant changes were observed at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. in maximum blood pressure for the 1st and 2nd overnight stay and at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 3rd and at 2 a.m., 4 a.m., 6 a.m. for the 4th and, respectively, average blood pressure at 10 p.m., 12 p.m. for the 1st and 2nd and at 10 p.m. for the 3rd and 4th, minimum blood pressure at 10 p.m., 12 p.m. from 1st to 4th, pulse rate at 2 a.m., 4 a.m., 6 a.m. from 1st to 3rd and at 2 a.m., 4 a.m. for the 4th, TP-KS at 10 p.m., 12 p.m., 2 a.m., 4a.m., 6 a.m. for the 1st and at 10 p.m., 2 a.m., 4 a.m., 6 a.m. for the 2nd and at 2 a.m., 4 a.m., 6 a.m. for the 3rd and at 6 a.m. for the 4th, PRP at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 2nd and 3rd and at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 4th, TPR at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. from 1st to 4th. As mentioned obove, the effects of Wangttum and Sibjeondaebotang on hemodynamics in sleep deprivation were observed both the impulse of SIM-YANG and mutual function of QI-HYOL. The effects of Magnetic water and Magnetic field were observed the side of mutual function of QI-HYOL.

• 한국운동역학회지
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• 제17권1호
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• pp.145-153
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• 2007

The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

• 미생물학회지
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• 제13권3호
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• pp.101-108
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• 1975

Physiological effects of 2,4-D on the growth of Chlorella ellipsoidea were investigated culturing the alage in the MN4 media containing 0. $10^{-4}/M$ and $4<\times}10^{-4}M$ 2,4-D. During 6 days culture were taken to analysis with respect to overall growth, photosynthesis, respiration and chemical composition. Results obtained from the experiment were as follows : 1) The growth of chlorella was increased at $10^{-4}M$ and decreased at $4{\times}10^{-4}M$ of 2,4-D concentrations 2) At $10^{-4}M$ pf 2,4-D cpncentration, the activity of photosynthesis enhanced relative to contro. while at $4{\times}10^{-4}M$ it was not changed. In both concentrations, however, the rate of respiration was down from the control. 3) At $10^{-4}M$ 2,4-D, the concentration of carbondrate metabolites was not changed relative to control, while significant increase in the concentrations of proteins and nucleic acids was observed. On the other hand at $4{\times}10^{-4}M$ of 2,4-D concentrations, all the metabolites including carbohydrates, proteins and nucleic acids were descreased. 4) It is concluded that 2,4-D at $10^{-4}M$ concentration accelerates the growth of chlorella by promoting the activities of photosynthesis and biosynthesis of proteins and nucleic acids.

• 동아시아식생활학회지
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• 제14권6호
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• pp.608-617
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• 2004

This study is conducted to find out the appropriate preparing method and fermenting condition through analysis of physicochemical, microbiological, sensory evaluation of Myungtae sikhae, Korean fermented fishery food which was prepared in simplified and conventional methods. During the fermentation of sikhae at 4℃, pH was decreased gradually, but acidity was increased and salinity was steady state. In this aspect, no significant difference was revealed between sikhae prepared in simplified and conventional methods. Under fermentation at 20℃ and kept at 4℃, pH was decreased to 4.6～4.9 for 15～20 days fermentation with steady state of salinity. However, acidity was changed in conversely. The content of reducing sugar with rapidly decreasing in the beginning of fermentation was continually decreased. The propagation of microorganism in th sikhae depends on the fermentation temperature rather than the preparing methods. Generally low propagation of microorganism began to increase gradually in the sikhae fermented at 4℃ comparing to that fermented at 20℃. In the sikhae fermented at 20℃ and kept at 4℃, the total count including lactic acid bacteria, was rapidly increased after 5 days' fermentation of sikhae is dominated by Lactobacillus sp. and the unique flavor and organic acid were contributed by this flora with Leuconostoc. sp. By the sensory evaluation, the best flavor was revealed in the sikhae, prepared in simplified method fermented for 30 days at 4℃. Until 20 days, sikhae fermented at 20℃ revealed higher score than fermented at 4℃. General preference for the sikhae fermented at 4℃ for 15 days prepared by simplified method and those fermented at 20℃ for 10 days. The sikhae, fermented at 4℃, showed less difference between preparing methods and preferable acceptability after being 25 days. The best acceptability of sikhae fermented at 20℃ showed after 15～25 days. In the final conclusion, the appropriate preparing method of sikhae is to use frozen Alaska pollack with simplified method and fermented at 20℃ for 5 days and then kept at 4℃.

• 한국식품조리과학회지
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• 제9권1호
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• pp.43-51
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• 1993

Buckwheat protein isolate was tested for the effects of pH, addition of sodium chloride and heat treatment on solubility, emulsion capacities, emulsion stability, surface hydrophobicity, foam capacities and foam stability. The solubility of buckwheat protein isolate was affected by pH and showed the lowest value at pH 4.5, the isoelectric point of buckwheat protein isolate. The solubility significantly as the pH value reached closer to either ends of the pH, i.e., pH 1.0 and 11.0. The effects of NaCl concentration on solubility were as follows; at pH 2.0, the solubility significantly decreased when NaCl was added; at pH 4.5, it increased above 0.6 M; at pH 7.0 it increased; and at pH 9.0 it decreased. The solubility increased above $80^{\circ}C$, at all pH ranges. The emulsion capacity was the lowest at pH 4.5. It significantly increased as the pH approached higher acidic or alkalic regions. At pH 2.0, when NaCl was added, the emulsion capacity decreased, but it increased at pH 4.5 and showed the maximum value at pH 7.0 and 9.0 with 0.6 M and 0.8 M NaCl concentrations. Upon heating, the emulsion capacity decreased at acidic pH's but was maximised at pH 7.0 and 9.0 on $60^{\circ}C$ heat treatment. The emulsion stability was the lowest at pH 4.5 but increased with heat treatment. At acidic pH, the emulsion stability increased with the increase in NaCl concentration but decreased at pH 7.0 and 9.0. Generally, at other pH ranges, the emulsion stability was decreased with increased heating temperature. The surface hydrophobicity showed the highest value at pH 2.0 and the lowest value at pH 11.0. As NaCl concentrationed, the surface hydrophobicity decreased at acidic pH. The NaCl concentration had no significant effects on surface hydrophobicity at pH 7.0, 9.0 except for the highest value observed at 0.8 M and 0.4 M. At all pH ranges, the surface hydrophobicity was increased, when the temperature increased. The foam capacity decreased, with increased in pH value. At acidic pH, the foam capacity was decreased with the increased in NaCl concentration. The highest value was observed upon adding 0.2 M or 0.4 M NaCl at pH 7.0 and 9.0. Heat treatments of $60^{\circ}C$ and $40^{\circ}C$ showed the highest foam capacity values at pH 2.0 and 4.5, respectively. At pH 7.0 and 9.0, the foam capacity decreased with the increased in temperature. The foam stability was not significantly related to different pH values. The addition of 0.4 M NaCl at pH 2.0, 7.0 and 9.0 showed the highest stability and the addition of 1.0 M at pH 4.5 showed the lowest. The higher the heating temperature, the lower the foam stability at pH 2.0 and 9.0. However, the foam stability increased at pH 4.5 and 7.0 before reaching $80^{\circ}C$.

• 환경생물
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• 제23권1호
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• pp.52-56
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• 2005

8일간 생장한 보리 유식물에 72시간 동안 dimethipin을 처리하면서 엽록소 함량의 변화와 광합성적 전자전달 활성을 측정하였다. 10/sup -3/M dimethipin을 72시간 처리한 경우 엽록소 함량이 33% 감소하였다. 이에 비해 7일간 암소에서 생장시킨 후 dimethipin을 처리한 보리 유식물은 녹화 48시간에 10/sup -4/M에서 대조구에 비해 43% 엽록소 함량이 감소하였으며, 엽록소 a/b 비율이 증가하였다. 24시간의 dimethipin처리는 PSⅡ+PSⅠ활성에 거의 영향을 미치지 않았다. 10/sup -4/M dimethipin처리는 48시간 배양시 10% 그리고 72시간에는 25%의 PSⅡ+PSⅠ 활성을 억제하였다. Water splitting system을 제외한 PSⅡ+PSⅠ 활성은 10/sup -4/M dimethipin을 48시간 처리할 경우 16% 그리고 72시간에는 27% 억제되었다. 10/sup -4/M dimethipin을 24시간 처리할 경우 PSⅡ활성은 8%, 48시간에서는 13% 그리고 72시간에서는 18% 억제되었다. 10/sup -4/M dimethipin을 24시간 처리할 경우 PSⅠ활성은 4%, 48시간에서는 8% 그리고 72시간에서는 10% 억제되었다. 7일간 암소에서 자란 황백화된 보리 유식물을 녹화시간 6, 12, 24와 48시간에 dimethipin을 처리할 경우 PSⅡ+PSⅠ 활성은 5, 10, 10 그리고 11% 억제되었다. 반면에 water splitting system을 제외한 PSⅡ+PSⅠ 활성은 모든 처리시간에서 그 효과가 없었다. 또한 PSⅡ와 PSⅠ의 활성도 대조구와 큰 차이가 없었다. 이와 같이 dimethipin에 의해 야기된 광합성적 전자전달활성의 억제는 엽록체 합성과정에서의 영향보다는 엽록체의 기능에 영향을 주는 것으로 사료되며, 이러한 엽록체 기능의 감소는 dimethipin에 의해 야기되는 30∼40%의 엽록소 함량감소에 의한 것으로 사료된다.

• Journal of Acupuncture Research
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• 제22권3호
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• pp.169-183
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• 2005

Cobrotoxin의 항암효과를 알아보고자 암세포인 PC-3 cell에 cobrotoxin을 처리한 후 cell viability, cell death, apoptosis, cell cycle 및 관련단백질, Adk, 및 MAP kinase 관련 단백질의 변화를 관찰하여 다음과 같은 결론을 얻었다. 1. PC-3 세포에 각각 cobrotoxin을 각각 0-16 nM까지 투여시 대조군에 비하여 모두 농도 의존적으로 세포들의 모양이 길죽한 나선형 모양에서 둥글게 응축되는 모습으로 변하였으며 고농도로 갈수록 세포들의 성장이 억제를 보였다. 2. MTT assay를 이용하여 세포 생존력을 측정 한 결과, 0.1, 1 및 4nM의 cobrotoxin 처리군은 정상군에 비하여 세포활성의 감소를 나타내었고, 8, 16nM의 cobrotoxin 처리군은 정상군에 비하여 세포활성의 유의한 감소를 보였다. 3. PC3-cell에 cobrotoxin을 처리한후 FACS analysis를 통하여 세포주기를 측정한 결과 세포주기 중 S phase에서 0.0lnM cobrotoxin 처리군은 변함이 없지만, 1, 2, 4, 8 및 16 nM 의 cobrotoxin 처리군에서는 정상군에 비해 감소를 보였다. G2-M phase에서 0.1, 1, 2, 4, 8 및 16M의 cobrotoxin 처리군에서 정상군 에 비하여 증가를 보였다. 4. Cobrotoxin 처리후 Cox-2의 발현을 48시간 관찰한 결과 12시간에서 최대치를 이루었고, 6, 12 및 24시간후에서 대조군에 비하여 유의한 감소를 나타내었다. 5. G1 phase에서 활성을 이루는 Cdk4, cyclin Dl의 발현을 살펴본 결과 Cdk4는 cobrotoxin 1, 2, 4 및 8nM 처리군에서 정상군에 비하여 농도 의존적으로 감소하였고, cobrotoxin 4, 8nM 처리군은 정상군에 비하여 유의한 감소를 나타내었다. Cyclin Dl은 cobrotoxin 1, 2, 4 및 8nM 처리 군에서 정상군에 비하여 농도 의존적으로 감소하였다. Cycline E는 cobrotoxin 1, 2, 4 및 8nM 처리 군에서 정상군에 비해 큰 변화가 없었다. G2/M phase에 관여하는 단백질인 Cyclin Bl 은 cobrotoxin 1, 2, 4 및 8nM 처리군에서 정상군에 비하여 농도 의존적으로 감소하였고, cobrotoxin 2, 4, 8M 처리군은 정상군에 비하여 유의한 감소를 나타내었다. 6. 세포성장 단백질인 Akt의 발현을 살펴본 결과 1, 2, 4 및 8nM의 cobrotoxin 처리군에서 정상군에 비하여 농도 의존적으로 감소하였고, 4, 8nM친 cobrotoxln 처리군은 정상에 비하여 유의한 감소를 나타내었다. 7. MAP kinase에 관여하는 단백질인 ERK, p-ERK, JNK, p-JNK p38, p-p38에 미치는 영향을 살펴본 결과, ERK은 1, 2nM의 cobrotoxin 처리군에서 정상군에 비하여 증가를, 4, 8nM의 cobrotokin 처리군에서 정상군에 비하여 감소를 나타내었다. p-ERK은 1, 2, 4nM의 cobrotoxin 처리군에서 정상군에 비하여 증가를, 8nM의 cobrotoxin 처리군에서 정상군에 비하여 감소를 나타내었다. JNK와 p-JNK는 1, 2, 4 및 8nM의 cobrotoxin 처리군에서 정상군에 비하여 증가를 나타내었다. p38는 1, 4, 8nM의 cobrotoxin 처리군에서 정상군에 비하여 감소를, 2nM의 cobrotoxin 처리군에서는 정상군에 비하여 감소를 나타내었다. p-p38는 1nM의 cobrotoxin 처리군에서 정상군에 비하여 증가를, 2, 4, 8nM의 cobrotoxin 처리군에서는 정상군에 비하여 유의한 감소를 나타내었다. 8. PC 3-cells 성장의 억제 역시 세포사멸을 유도한 세포성장억제 인지를 알아보기 위해 DAPI staining을 통한 세포의 핵을 염색하여 그 모양을 관찰한 결과, 정상세포가 동글동글 하고 균일한데 비하여 세포사멸이 일어난 세 포는 핵이 응축되고, 여러 조각으로 나뉜 모습을 관찰할 수 있었다. 또한, 세포사멸을 살펴본 결과 1, 2, 4, 8 및 16nM cobrotoxin 처 8nM의 cobrotoxin 처리군에서 정상군에 비하여 유의한 변동을 나타내지 않았다. Bcl-2 는 1, 2, 4 및 8nM의 cobrotoxin 처리군에서 정상군에 비해 농도 의존적으로 유의한 감소를 나타내었다. 9. 세포사멸 관련 유전자인 caspase family (caspase 3, 9)와 Bcl-2 family (Bcl-2, Bax), p53의 발현을 살펴본 결과, Bax는 1, 2, 4 및 Caspase 3과 9는 1, 2, 4nM의 cobrotoxin 처리군에서 정상군에 비하여 유의한 변동을 나타내지 않았으나, 8nM cobrotoxin 처리군에서는 유의한 감소를 나타내었다. 이상의 결과를 종합해보면, 일정수준(pico 또는 namo molar 수준)의 cobrotoxin이 prostate cancer cell의 성장을 억제하고, 세포사멸을 유도 하여 항암 효과가 있음을 확인할 수 있었으며 향후 cobrotoxin의 항암 효과를 실제 임상에 활용할 수 있게 되기를 기대한다.

• Korean Journal of Acupuncture
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• 제25권4호
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• pp.133-145
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• 2008

Objectives : The purpose of this study was to exam the effectiveness of acupuncture using the tonification & sedation manipulation for LI4(Hegu) and KI7(Fuliu) which was known to promote sweating for lack sweating and inhibit for excessive sweating. Methods : The subject of this study was 60 people who agreed with participating. The participants were divided into 6 groups as control group without acupuncture, acupuncture group with needle inserted perpendicularly at LI4, KI7 without manipulation(AT-A), acupuncture group with needle inserted and twisted at LI4 using sedation technique and KI7 using tonificaton technique(AT-B), acupuncture group with needle inserted and twisted at LI4 using tonificaton technique and KI7 using sedation technique(AT-C), acupuncture group with needle inserted and twisted at LI4 and KI7 using tonificaton technique(AT-D) and acupuncture group with needle inserted and twisted at LI4 and KI7 using sedation technique(AT-E). We obtained baseline data at a stable condition with the temperature of $25^{\circ}C$. After having the participant stay at $40^{\circ}C$ for 10 minute and treated with acupuncture for 10 minute, we measured the parameters such as sweating rate, water content in subcutaneous, water content in skin surface, systolic blood pressure, diastolic blood pressure, pulse rate, oxygen saturation and body temperature. Results : After the tonification & sedation manipulation at LI4 and KI7, the sweating rate was significantly decreased in AT-B, AT-D and AT-E groups. The water contents in skin surface was significantly decreased in AT-B and AT-E groups. The water contents in subcutaneous was significantly decreased in AT-B, AT-C, AT-D and AT-E groups. However, there were no significant changes in systolic blood pressure, diastolic blood pressure, pulse rate, oxygen saturation and body temperature. Conclusions : Acupuncture at LI4 and KI7 is effective in controling sweating, and the effect varies according to the tonification & sedation manipulation technique.

• 한국자동차공학회논문집
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• 제6권2호
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• pp.1-11
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• 1998

The 4WS system is usually developed to improve the maneuverability at low speed and the straight line stability at high speed, but it is found to have the severe understeer characteristics at high speed. Therefore a 4WS vehicle requires to turn the steering wheel much more than a 2WS vehicle at high speeds even a driver goes through the same curved road. In this study, to enhance the cornering performance of the 4WS vehicle at high speed, a DYC 4WS system is proposed based on the nonlinear 4WS system and direct yaw moment control system. Especially the proposed DYC 4WS system is able to realize a zero side slip angle for vehicles and a cornering performance similar to the 2WS vehicle at high speed.