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Estrous Response and Fertility in Postpartum Suckled Female Yaks (Bos grunniens) Treated with an Intravaginal Device Containing Progesterone(CIDR), Pregnant Mares' Serum Gonadotrophin and Prostaglandin Analogue

  • Zi, X.D.;Chang, S.;Lu, H.;Wang, X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.4
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    • pp.470-474
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    • 2005
  • The efficiency of a short-term treatment with an intravaginal device containing progesterone (CIDR) combined with pregnant mares' serum gonadotrophin (PMSG) and prostaglandin analogue ($PGF_{2\alpha}$) was evaluated for the induction of estrus, initiation of cyclic activity, and fertility in postpartum suckled yak cows. Seventy-five postpartum suckled yak cows were assigned to three treatments: (1) insertion of a CIDR intravaginal progesterone (1.9 g) (day 0), an administration of $PGF_{2\alpha}$ (0.2 mg i.m.) on day 6 and PMSG (1,000 IU i.m.) at the time of CIDR withdrawal on day 7 (CPP group, n=28); (2) an administration of $PGF_{2\alpha}$ (0.2 mg i.m.) on day 6 and PMSG (1,000 IU i.m.) on day 7 (PP group, n=21); (3) untreated animals served as the control (CG group, n=26). Seven yak bulls were placed in pastures with the cows for natural mating. Estrus rate in the CPP group (28/28) was higher (p<0.01) than in the PP group (6/21) and in the CG group (0/26) within 96 h after the end of treatment. The first service conception rate in the CPP group (21/28) was higher (p<0.01) compared with in the PP group (2/9) as judged by serum $P_4$ concentration $\geq$2.35 ng/ml on day 21 after breeding. It is concluded that a short-term progesterone treatment combined with PMSG and prostaglandin increased the proportion of yak cows that exhibited behavioral estrus with more synchronized estrus response and satisfactory conception rate in postpartum suckled yak cows.

Relationship among Savoring, Emotion and Happiness of Hores-Riding participants (승마참여자들의 향유하기, 정서 및 행복감)

  • Yang, Sung-Jee;Yi, Jae-Hoon
    • Journal of Convergence for Information Technology
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    • v.8 no.4
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    • pp.99-108
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    • 2018
  • This study regards the savoring, emotion and happiness of 232 equestrians who participated in leisure sports horse riding. First, exploratory factor analysis and confirmatory factor analysis were conducted to extract the main variables and to verify the validity of the study. Second, correlation analysis was performed to find out the correlation between major variables. Third, path analysis was performed to derive the final result of this study. In addition, we conducted a reliability analysis of major measurement tools to determine internal consistency(Conbach's ${\alpha}$) utilizing IBM SPSS 21.0 and IBM AMOS 21.0. The results show that past recall had a statistically significant negative impact on negative emotions and future expectations had a negative impact on positive emotions. Also, future expectations showed a statistically significant effect on euphoria and positive emotions had positive effects on happiness.

Characteristics of Sweet and Super Sweet Corn Seeds Shelled at Different Seed Moisture and Threshing Method Conditions (단옥수수와 초당옥수수 탈곡 시 종자 수분함량과 탈곡방법에 따른 종자 특성)

  • Lee, Suk-Soon;Yun, Sang-Hee;Yang, Seung-Kyu;Hong, Seung-Beom
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.7
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    • pp.632-638
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    • 2006
  • Characteristics of sweet (sugary, su) and super sweet (shrunken-2, sh2) corn seeds shelled by different threshing methods at different moisture content status were studied. Hybrid seeds of a su (Early Sunglow ${\times}$ Golden Cross Bantam 70, GCB 70) and a sh2 (Xtrasweet 82 ${\times}$Fortune) were dried to moisture content of 12, 15, 18, and 21%. Hand shelling did not give any mechanical damages to seeds, while an electrical corn thresher gave some visible mechanical damages. The emergence rate of hand shelled seeds was higher than that of machine shelled seeds by $6{\sim}14%$ for a su and by $9{\sim}18%$ for a sh2 hybrid depending on seed moisture contents in cold test. The optimum seed moisture content to reduce mechanical threshing damages and to improve seed quality was 15% for su and 12% for sh2 hybrid seeds. At the optimum seed moisture contents, germination rate at $25^{\circ}C$, emergence rate in the cold test and ${\alpha}-amylase$ activity were highest, while the percentage of damaged seeds and leakage of total sugars and electrolytes in soaking water were minimized.

Purification and Characterization of Branching Specificity of a Novel Extracellular Amylolytic Enzyme from Marine Hyperthermophilic Rhodothermus marinus

  • Yoon, Seong-Ae;Ryu, Soo-In;Lee, Soo-Bok;Moon, Tae-Wha
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.457-464
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    • 2008
  • An extracellular enzyme (RMEBE) possessing ${\alpha}-(1{\rightarrow}4)-(1{\rightarrow}6)$-transferring activity was purified to homogeneity from Rhodothermus marin us by combination of ammonium sulfate precipitation, Q-Sepharose ion-exchange, and Superdex-200 gel filtration chromatographies, and preparative native polyacrylamide gel electrophoresis. The purified enzyme had an optimum pH of 6.0 and was highly thermostable with a maximal activity at $80^{\circ}C$. Its half-life was determined to be 73.7 and 16.7 min at 80 and $85^{\circ}C$, respectively. The enzyme was also halophilic and highly halotolerant up to about 2M NaCl, with a maximal activity at 0.5M. The substrate specificity of RMEBE suggested that it possesses partial characteristics of both glucan branching enzyme and neopullulanase. RMEBE clearly produced branched glucans from amylose, with partial ${\alpha}-(1{\rightarrow}4)$-hydrolysis of amylose and starch. At the same time, it hydrolyzed pullulan partly to panose, and exhibited ${\alpha}-(1{\rightarrow}4)-(1{\rightarrow}6)$-transferase activity for small maltooligosaccharides, producing disproportionated ${\alpha}-(1{\rightarrow}6)$-branched maltooligosaccharides. The enzyme preferred maltopentaose and maltohexaose to smaller maltooligosaccharides for production of longer branched products. Thus, the results suggest that RMEBE might be applied for production of branched oligosaccharides from small maltodextrins at high temperature or even at high salinity.

A Study on the Curvature Ratio and Coefficient in Channel Bend (유로만곡부(流路彎曲部)의 곡율비(曲率比)와 곡율계수(曲率係數)에 관한 연구(硏究))

  • Chung, Yong Tai;Lee, Jin Eun;Song, Jai Woo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.11 no.1
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    • pp.117-124
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    • 1991
  • Based on the momentum equation for the flow in a stream bend, the force per unit area which the flow exerts on the outer of a bend is directly proportional to a certain curvature coefficient, $C{\alpha}$. This coefficient is dependent on the ratio of bend radius(R) to flow width(W), as well as on the coefficient of dynamic bedload friction, $tan{\alpha}$. According to the results of the data analysis for the downstrream at the Han river, the range of R/w values is between 2.0 and 4.0. Exploring the variations of $C{\alpha}$ with R/w values a functional relationship which, for the known values of $tan{\alpha}$, shows maximum values of $C{\alpha}$ for R/w values between 2.21 and 4.42 in 1963, while in 1981 its values lied between 1.93 and 3.54.

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Effect of Using Progesterone Releasing Intravaginal Device with Ovsynch Program on Reproduction in Dairy Cattle during Summer Season

  • Alnimer, M.;Lubbadeh, W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.9
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    • pp.1268-1273
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    • 2003
  • Sixty postpartum lactating Friesian cows in 3 treatments at a commercial dairy farm were used to study the effect of using progesterone supplementation with GnRH and PGF2$\alpha$ synchronization with and without timed AI on fertility during summer. Cows in treatment1($Tr_1$) and treatment2 ($Tr_1$) were fitted with progesterone releasing intravaginal device (PRID) device and injected with 10 g GnRH agonist on $51{\pm}3$ d postpartum (pp). Seven days later, PRID was removed and cows received 25 mg PGF2$\alpha$. Two days later, $Tr_1$ cows received another injection of 10 g GnRH and timed AI 16-20 h later. Control cows received only 25 mg PGF2$\alpha$ $58{\pm}3d\;pp$. $Tr_2$ and control cows were AI at detected estrus. Serum progesterone for all cows was determined on days of injection, AI and 21, 23 and 28 d postinsemination. Pregnancy rates from first AI based on serum P4 concentrations on d 21, 23 and 28 postinsemination (50, 40 and 35%) and that based on rectal palpation 40-45 d postinsemination (30, 15 and 15% for $Tr_1$, $Tr_2$ and control cows, respectively) did not differ among the three groups. Whereas, pregnancy rate at 120 d pp for $Tr_1$ (65%) was higher (p<0.05) than that in $Tr_2$ (30%) or control (30%). The overall pregnancy rate was not significantly different (90, 90 and 75% for $Tr_1$, $Tr_2$ and control, respectively). Days open for cows in $Tr_1$ ($100.3{\pm}9$) was less (p<0.03) than that in $Tr_2$ ($130.9{\pm}9$) or control ($135.1{\pm}10$). Results indicate that using PRID device with Ovsynch program had significantly increased pregnancy rate and decreased days open compared to AI at detected estrus after synchronization with GnRH, PRID and PGF2$\alpha$ or synchronization with one injection of PGF2$\alpha$.

T0901317 as an Inhibitor of Transcriptional Activation of Constitutive Androstane Receptor (CAR) (Constitutive androstane receptor (CAR)의 전사활성 저해제로서의 T0901317)

  • Kim, Hyun-Ha;Seol, Won-Gi
    • Journal of Life Science
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    • v.21 no.4
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    • pp.481-485
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    • 2011
  • T0901317 is a potent synthetic ligand for liver X receptor (LXR, NR1H2/3), a member of the nuclear receptor superfamily that functions as a transcription factor. However, T0901317 has been also reported to modulate the activity at least four other nuclear receptors (NRs), acting as agonists for farnesoid X receptor (FXR, NR1H4) and pregnane X receptor (PXR, NR1I2) and as antagonists for androgen receptor (AR, NR3C4) and retinoid-related orphan receptor-${\alpha}$ (ROR-${\alpha}$, NR1F1). We report here that T0901317 can also function as an inhibitor for constitutive androstane receptor (CAR, NR1I3). Since CAR is a major player of xenobiotic and cholesterol metabolism in the liver, along with PXR, FXR and LXR, which are reported to be regulated by T0901317, this further complicates the interpretation of potential results with T0901317 in liver cells.

A Study of Improvement of Skin Condition and Sensory Efficacy by Periodic Application of L-α-Amino Acid (L-α-아미노산의 주기적 도포에 의한 피부개선 효능과 소비자 체감 효능 연구)

  • Kwon, Koo Chul;Lee, Sung Woo;Ahn, Byungjun;Kang, Nae-Gyu;Park, Sun Gyoo;Park, Sang Wook
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.1
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    • pp.9-17
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    • 2019
  • In this study, $L-{\alpha}-amino$ acid, which is a constituent of natural moisturizing factor, was applied to skin in periodic cycle to improve skin tone and texture roughness. Based on the polarity of the alkyl group (R) of the $L-{\alpha}-amino$ acid, the acid was categorized into two groups and their efficacy was studied. As a result, it was found that the improvement rate of $L-{\alpha}-amino$ acid complex with polar alkyl group ($L-{\alpha}-AAC-1$) is 21% higher than that of $L-{\alpha}-amino$ acid complex with non-polar alkyl group ($L-{\alpha}-AAC-2$). For clinical trials, emulsions containing $L-{\alpha}-amino$ acid complex ($L-{\alpha}-AAC-1$) were applied to the randomly selected 20 to 40 year old female participants, as an experimental group, on the right facial cheek once per day for 8 weeks, and emulsions without $L-{\alpha}-amino$ acid complex, as a control group, were applied to the left facial cheek in the same way. Improvements in skin tone were measured using $JANUS^{(R)}$ equipment and analyzed using image analysis software. Skin texture improvement was measured and analyzed mechanically using the phaseshift rapid in-vivo measurement of the skin (PRIMOS) equipment. As a result, improvements of skin tone and skin texture were 11.7% and 6.7%, respectively. In addition, a questionnaire survey was conducted to the participants on the aesthetic improvement and the degree of the feeling of skin improvement. The results suggest that $L-{\alpha}-amino$ acid can be used as a cosmetic substance that can provide aesthetic satisfaction through physiological skin tone and texture roughness.

New Methods for Isolation of Sesquiterpene from Panax ginseng (인삼 Sesquiterpene의 새로운 분리방법)

  • 위재준;신지영
    • Journal of Ginseng Research
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    • v.21 no.3
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    • pp.214-218
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    • 1997
  • New simple methods for the Isolation of sesquiterpenes from Panax ginseng were developed. First, volatile compounds were isolated by simultaneous distillation and extraction (SDE) with 30% methanol and $\alpha$-hexane instead of water and ethyl ether/pentane (1:1). Secondly, head space volatiles in U-shaped tube at 7$0^{\circ}C$ were passed through C18 Sep-Pak by nitrogen gas streaming and the adsorbed volatiles were fluted by $\alpha$-hexane. TLC analysis showed that the volatile concentrates consisted mainly of terpenes when colored by vanillin-sulfuric and. GC/MS data revealed that approximately 30 sesquiterpenes of molecular weight 204 occupied 81.1% or more of the volatile concentrates isolated by those two newly developed methods. Among these, alloaromadendrene, germacrene B, isocaryophyllene, $\alpha$-neoclovene, ${\gamma}$-muurolene, $\beta$-panaslnsene, and $\alpha$-humulene were identified as being major sesqulterpenes by authentic samples or literatme search Key words : Panax ginseng, volatile compound, sesquiterpene, isolation, new method, GC/MS.

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PERI-NATAL AND POST-NATAL STUDY OF THE RECOMBINANT HUMAN INTERFERON ${\alpha}A\;(rHuIFN-{\alpha}A)$ IN RATS

  • Lee, Yong-Soon;Kim, Yun-Bae;Kim, Hyun-Su;Cho, Nam-Jin;Yoo, Moo-Young
    • Toxicological Research
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    • v.3 no.1
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    • pp.55-63
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    • 1987
  • A Peri-and Postnatal Study was Carried out to examine the effects of rHuIFN-${\alpha}A$, produced by gene-manipulated E. coli, on offsprings of Wistar rats. The substance was administered intraperitoneally to dams at dose levels of $1{\times}10^5$, $4{\times}10^5$ and $1.2{\times}10^6$ I.U/kg/day during the period from day 17 of gestation to day 21 after delivery. All the pregnant dams were allowed to deliver naturally, and the postnatal development of the offsprings was observed. No noticeable toxic effects and pathological changes on dams were observed, and no detectable variations in postnatal development of offsprings occured.

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