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Determination of Total Content of Phenolic Compounds in Chinese Matrimony Vine's Accessions (국내외 수집종 구기자 잎과 줄기의 페놀화합물 함량)

  • Cho, Jin-Woong;An, Tae-Hwan;Lee, Suk-Young;Park, Kee Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.4
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    • pp.409-417
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    • 2012
  • This study was conducted to determine the variation of phenolic compounds in the leaf and stem of 131 accessions of Lycium chinesis Miller. The levels of total phenolic compounds in the leaf of L. chinesis ranged between 8.8 to $14.9mg\;g^{-1}$ and among them 60% of the accessions belong between 11.6 and $13.5mg\;g^{-1}$ for the content of phenolic compounds in the leaf. The accession CB03286-89 contained the highest total phenolic compounds among the accessions tested, which was 1.7-fold higher than that of the lowest content accession CBP03310-250. In the stem, the total phenolic compound of 131 accessions of L. chinesis ranged from 6.8 to $12.4mg\;g^{-1}$, showing slightly lower level than that in the leaf. The content of (+)catechin was highest in the leaf and stem of accession CB03286-89 and Japan No.1, respectively. Myricetin was detected in the leaf of seven accessions (i.e. Geumsan jaerae, Japan No.1, China collection No.1, CL32-13, CB04329-13, China collection No.12 and CB03286-89) and in the stem of five accessions (i.e., Japan No.1, China collection No.1, China collection No.12, CB03286-89 and 99797). Accessions had a great influence on the content of phenolic compounds. So, accessions-specific phenolic compound profiles might be helpful for commercial use or production of phenolic compounds in L. chinesis.

Development of the Holocene Sediments in Gamak Bay of the South Sea, Korea (남해 가막만의 현생퇴적층 발달특성)

  • Kim, So Ra;Lee, Gwang Soo;Choi, Dong Lim;Kim, Dae Choul;Lee, Tae Hee;Seo, Young Kyo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.2
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    • pp.131-146
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    • 2014
  • High-resolution seismic profiles coupled with sediment sampling were analyzed to investigate the acoustic characters and distribution patterns of the late Holocene sediments in Gamak Bay of the South Sea, Korea. The mean grain size of surficial sediment lies around $6.3{\sim}9.7{\Phi}$. Sediments in the bay consist of silt and clay with progressive decrease toward the inner bay. The seismic sedimentary sequence overlying the acoustic basement can be divided into two sedimentary units (GB I and II) by a prominent mid-reflector (Maximum Flooding Surface; MFS). The acoustic basement occurs at the depth between 20 m and 40 m below the sea-level and deepens gradually southward. The GB I, mostly occupying the channel-fill, is characterized by reflection-free seismic facies. It can be formed as late Transgressive System Tract (TST), interpreted tidal environment deposits. MFS appears at the depth of about 15~28 m below the sea-level and is well defined by even and continuous reflectors on the seismic profile. The GB II overlying MFS is composed of acoustically transparent to semitransparent and parallel internal reflectors. GB II is interpreted as the Highstand System Tract (HST) probably deposited during the last 6,000 yrs when the sea level was close to the present level. Especially, it is though that the GB II was subdivided into two layers (GB II-a and II-b) by a HST-reflector and this was classified by wind, sea water flux, and tidal current.

Three Crystal Structures of Dehydrated $Ag_{12-x}Na_x-A$ (x = 4, 6, and 8) Treated with Rubidium Vapor (탈수한 $Ag_{12-x}Na_x-A$ (x = 4, 6, 및 8)를 루비듐 증기로 처리한 세가지 결정구조)

  • Lee, Hyeon Do;Kim, Un Sik;Park, Jong Yeol;Kim, Yang
    • Journal of the Korean Chemical Society
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    • v.38 no.3
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    • pp.186-196
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    • 1994
  • Three fully dehydrated partially $Ag^+$-exchanged zeolite A(Ag_4Na_8-A, Ag_6Na_6-A, and Ag_8Na_4-A) were treated at $250^{\circ}C$ with 0.1 torr Rb vapor at 4 h. Their structures were determined by singlecrystal X-ray diffraction methods in the cubic space group $Pm{\bar3}m$ (a = 12.264(4) $\AA$, a = 12.269(1) $\AA$, and a= 12.332(3) $\AA$, respectively) at $22(1)^{\circ}C$, and were refined to the final error indices, R(weighed), of 0.056 with 131 reflections, 0.068 with 108 reflections, and 0.070 with 94 reflections, respectively, for which I > $3\sigma(I).$ In these structures, Rb species are found at three different crystallographic sites; three $Rb^+$ ions per unit cell are located at 8-ring centers, ca. 6.0∼6.8 $Rb^+$ ions are found opposite 6-rings on threefold axes in the large cavity, and ca. 2.5 $Rb^+$ ions are found on three fold axes in the sodalite unit. Also, Ag species are found at two different crystallographic sites; ca. 0.6∼1.0 $Ag^+$ ion lies opposite 4-rings and about 1.8∼4.2 Ag atoms are located near the center of the large cavity. In these structures, the numbers of Ag atoms per unit cell are 1.8, 3.0, and 4.2, respectively, and these are likely to form hexasilver clusters at the centers of the large cavities. The $Rb^+$ ions, by blocking 8-rings, may have prevented silver atoms from migrating out of the structure. Each hexasilver cluster is stabilized by coordination to 6-ring, 8-ring $Rb^+$ ions, and also by coordination to a 4-ring $Ag^+$ ion.

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Primary Productivity and Assimilation Number in the Kyonggi Bay and the mid0eastern coast of Yellow Sea (서해 중동부 연안수역과 경기만에서 일차 생사력과 동화계수에 관한 연구)

  • 강연식;최중기
    • 한국해양학회지
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    • v.27 no.3
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    • pp.237-246
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    • 1992
  • In order to examine controlling factors on primary productivity and assimilation Number of phytoplankton, chlorophyll-a concentrations, light intensity, temperature, salinity and transparency were measured in the Kyonggi Bay and in the mid0eastern coast of Yellow Sea from March 1989 to October 1990. Chlorophyll-a concentration of phytoplankton ranged from 0.91 to 4.30 ug/; in the Kyonggi Bay, and from 0.78 to 4.97 ug/l in the mideastern coast of Yellow Sea. Daily averaged primary productivities and annual primary productivities of phytoplankton ranged from 37.23 to 1104.44 (averaged 361.54) mgC/m$^2$/day, 131.96hC/m$^2$/yr in the mid0eastern coast of Yellow Sea, respectively. Assimilation Number of phytoplankton ranged from 1.47 to 28.28 mgC/mg chl-a/hr in the Kyonggi Bay, and of phytoplankton in the Kyonggi Bay was higher than that of the mid0eastern coast of Yellow Sea. Light utilization efficiencies (a) in the P-I curve ranged from 0.03 to 0.93 [mgC/mg chl-a/hr]/[ue/m$^2$/sec]in the Kyonggi Bay, and from 0.01 to 0.62 [mgC/mg chl-a/hr]/[ue/m$^2$/sec] in the mid-eastern coast of Yellow Sea. Their results indicated that phytoplankton in the Kyonggi Bay utilized light more efficiently than those of the mid0eastern coast of Yellow Sea. The average values of I/SUB k/ were 48.15 ue/m$^2$/sec in the Kyonggi Bay, and 120.37 uE/m$^2$/sec in the mid-eastern coast of yellow Sea. It means the phytoplankton populations in the Kyonggi Bay seem to be adapted to lower light intensity than those of the mid-eastern coast of Yellow sea.

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Comparison of Urine Iodine/Creatinine ratio between Patients following Stringent and Less Stringent Low Iodine Diet for Radioiodine Remnant Ablation of Thyroid Cancer (갑상선암의 방사성요오드치료를 위한 저요오드식이 방법 차이에 따른 소변 중 요오드/크레아티닌 비의 비교)

  • Roh, Jee-Ho;Kim, Byung-Il;Ha, Ji-Su;Chang, Sei-Joong;Shin, Hye-Young;Choi, Joon-Hyuk;Kim, Do-Min;Kim, Chong-Soon
    • Nuclear Medicine and Molecular Imaging
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    • v.40 no.6
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    • pp.322-326
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    • 2006
  • A low iodine diet (LID) for $1{\sim}2$ weeks is recommended for patients who undergoing radioiodine remnant ablation. However, the LID educations for patients are different among centers because there is no concrete recommendation for protocol of LID. In this investigation, we compared two representative types of LID protocols performed in several centers in Korea using urine iodine to creatinine ratio (urine I/Cr). Methods: From 2006, April to June, patients referred to our center for radioiodine remnant ablation of thyroid cancer from several local hospitals which had different LID protocols were included. We divided into two groups, stringent LID for 1week and less stringent LID for 2 weeks, then measured their urine I/Cr ratio with spot urine when patients were admitted to the hospital. Results: Total 27 patients were included in this investigation (M:F=1:26; 13 in one-week stringent LID; 14 in two-week less stringent LID. Average of urine I/Cr ratio was $127.87{\pm}78.52{\mu}g/g$ in stringent LID for 1 week, and $289.75{\pm}188.24{\mu}g/g$ in less stringent LID for 2 weeks. It was significantly lower in stringent LID for 1 week group (p=0.008). The number of patients whose urine I/Cr ratios were below $100{\mu}g/g$ was 6 of 13 in stringent LID for 1 week group, and 3 of 14 in less stringent LID for 2 weeks group. Conclusion: Stringent LID for 1 week resulted in better urinary I/Cr ratio in our investigation compared with the other protocol. However it still resulted in plenty of inadequate range of I/Cr ratio, so more stringent protocol such as stringent LID for 2 weeks is expected more desirable.

Prospective Morphometric Study for Orbital Floor and Medial Wall Thickness in Vivo (안와바닥과 내벽의 두께에 대한 전향적 생체 계측학적 연구)

  • Park, Chang Sik;Kim, Yong Kyu
    • Archives of Craniofacial Surgery
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    • v.10 no.2
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    • pp.67-70
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    • 2009
  • Purpose: Anatomical basis around orbit can be helpful in periorbital surgery, and there are many articles about measurement between periorbital reference points. In 1967, Jones and Evans measured the orbital wall thickness of Asian cadavers and this article has been cited more than 50 times. But there is no research in orbital thickness in Vivo. Author's idea was based on difference between live human and human cadaver. Material & Method: We conducted this study from 63 consecutive blow out fracture patients between January, 2000 to june, 2005 by collecting the bone fragments and measured the thickness of that fragment using vernia calipers. Anatomically, orbital floor is separated two area by inferior orbital fissure and we measured each area. Three areas were zone I (medial wall), zone II (medial to inferior orbital fissure) and zone III (lateral to inferior orbital fissure). Result: When the overall results were considered, the thickness of Zone I (medial wall of orbit) was average $0.131{\pm}0.006mm$ in male and $0.129{\pm}0.007mm$ in female and Zone II (medial side of orbital floor) was $0.251{\pm}0.005mm$ in male and $0.245{\pm}0.006mm$ in female, Zone III (lateral side of orbital floor) was $0.237{\pm}0.006mm$ in male and $0.226{\pm}0.006mm$ in female. There were no statistical difference between orbital wall thickness of male and female. Also, orbital wall thickness of adults measured $0.130{\pm}0.005mm$, $0.250{\pm}0.005mm$, $0.232{\pm}0.006mm$ in Zone I, Zone II, Zone III and $0.128{\pm}0.006mm$, $0.233{\pm}0.005mm$, $0.215{\pm}0.007mm$ in Zone I, Zone II, Zone III from childs, and there were no statistical difference between adult and child. Conclusion: This article is the first study about Korean orbital wall thickness, and can be helpful to periocular surgery.

The Study of Anti-inflammtory Mechanism with Bee Venom on Human Synoviocytes (인체(人體) 활막세포(滑膜細胞)를 대상으로 한 봉양침액(蜂藥鍼液)의 염증(炎症) 치료(治療) 기전(機轉) 연구(硏究))

  • Bae, Chul-woo;Song, Ho-sueb
    • Journal of Acupuncture Research
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    • v.21 no.3
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    • pp.121-131
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    • 2004
  • Objective : The purpose of this study was investigation how the bee venom(BV) prevents inflammation in human cell. Methods : we induced inflammation on human synoviocyte cell by lipopolysaccharide(LPS) and sodium nitroprusside(SNP), treated the bee venom and melittin on this cell, surveyed the expression of Nisotric oxide(NO), inducible nitric oxide synthase(iNOS), Cyclooxygenease-2(COX-2), cytolic phospholipase $A_2(cPLA_2)$, Prostaglandin $E_2(PGE_2)$ and nuclear factor-${\kappa}B$(NF-${\kappa}B$), and got below conclusions. Results : Compared with control 1. Expressions of LPS-induced $PGE_2$(BV 1, $5{\mu}g/m{\ell}$) and SNP-induced PGE2(BV 0.5, 1, $5{\mu}g/m{\ell}$)were decreased significantly. 2. Expressions of LPS-induced NO(BV 0.5, 1, $5{\mu}g/m{\ell}$) and SNP-induced NO(BV 1, $5{\mu}g/m{\ell}$)were decreased significantly. 3. Expressions of LPS-induced COX-2(BV 1, $5{\mu}g/m{\ell}$) and SNP-induced COX-2(BV $5{\mu}g/m{\ell}$)were decreased significantly. 4. Expressions of LPS-induced iNOS(BV 0.5, 1, $5{\mu}g/m{\ell}$) and SNP-induced iNOS(BV $5{\mu}g/m{\ell}$) were meanless by all dose. 5. Expressions of LPS-induced $cPLA_2$(BV 1, $5{\mu}g/m{\ell}$) and SNP-induced cPLA2(BV 1, $5{\mu}g/m{\ell}$)were decreased significantly. 6. Expressions of LPS-induced NF-${\kappa}B$(BV $5{\mu}g/m{\ell}$, melittin $5{\mu}g/m{\ell}$) and SNP-induced NF-${\kappa}B$(BV 0.5, 1, $5{\mu}g/m{\ell}$, melittin 5, $10{\mu}g/m{\ell}$)were decreased significantly. 7. Expressions of LPS-induced NF-${\kappa}B$ binding activity (BV $1{\mu}g/m{\ell}$, melittin $5{\mu}g/m{\ell}$, melittin $5{\mu}g/m{\ell}$+ DTT 20mM) were decreased significantly. Conclusion : The bee venom treatments on synoviocyte showed significant changes in LPS and SNP induced NO, iNOS, COX-2, cPLA2, PGE2 and NF-${\kappa}B$, these results suggest that bee venom is effective to inflammations and establish the process of bee venom therapy, so we expect active use of bee venom to control the inflammation.

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Effects of Sound Stress on Physiological Processes of the American Leafminer, Liriomyza trifolii, and Proteomic Analysis (스트레스 음파 처리에 따른 아메리카잎굴파리(Liriomyza trifolii)의 생리 변화와 프로테오믹 분석)

  • Park, Jung-A;Surakasi, Venkara Prasad;Kim, Yong-Gun
    • Korean journal of applied entomology
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    • v.50 no.2
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    • pp.131-139
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    • 2011
  • This study investigated the adverse effects of sound treatment on physiological processes of the American leafminer, Liriomyza trifolii, during several developmental stages. Larval feeding activity was analyzed by measuring feeding tunnel length. It was significantly suppressed by sound treatment (5,000 Hz, 95 dB). Sound treatment delayed the pupal period at 315 - 5,000 Hz and prevented adult emergence at 1,000 - 5,000 Hz. Female oviposition was also inhibited by the stress sound treatments. However, phototactic adult movement was not affected by sound treatment. Pupae treated with 5,000 Hz showed marked changes in protein patterns analyzed by two dimensional electrophoresis. MALDI-TOF analysis of specific protein spots indicated that trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MA1, and tropomyosin I (isoform M) were predicted in the control insects and disappeared in the insects treated with sound. By contrast, DOCK9, cytoskeletal keratin II, and F0F1-ATP synthase beta subunit were predicted only in the sound-treated insects. Furthermore, stress sound significantly increased the susceptibility of L. trifolii to insecticides. These results suggest that physiological processes of L. trifolii are altered by sound stress, which may be exploited to develop a novel physical control tactic against L. trifolii.

A Study on the Change of Silhouette (Mainly on Wonen's Costume) (Silhouette의 변천(變遷)에 관(關)한 고찰(考察) - 여자(女子)의복(衣服)을 중심(中心)으로 -)

  • Lee, Sun-Hong
    • Journal of the Korean Society of Costume
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    • v.1
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    • pp.131-150
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    • 1977
  • The new sense of costume is controlled by silhouette. The sense of the times is sensitively reflected in silhouette. For we can perceive the transition of the times through silhouette it is significant to know what course of changes the western costume, which almost became the international costume today, had been going through. I began with the definition and condition of silhouette in this study of silhouette. I took a general survey of silhouette study-ing various kinds of silhouette and the relation between the material and silhouette. I sought the factors which causec the changes in costume and also studies the process of the changes The process of the costume changes is studied by the order of ancient times, mediaeval times, mordern ages and present days. I selected one representative silhouette of women's costume of each period. The darpery form of the ancient time's costume became the tunic form and the tunic form became the tight tunic form today. From this we can perceive that the Gothic period was the limitation of westrn costume. It means that the ancient times was the period of drapery, the midiaeval times was the period of transition from tunic into tight tunic and the modern ages is the period of development of tight tunic. In Egyptian period thin materials were used for costume which was worn in exposed style. In Greek period the costume had the drapery style. The Roman's magnificent costume resembled the Greek's. The mediaeval costume was formed in Byzantium where the northern Europe style of costume was mixed with the gay oriental costume. The Romanesque and Gothic period followed the Byzantine period completing the midiaeval costume. Tight tunic is developed in modern ages. Italian fashion of tight tunic was the first fashion of the modern ages. Germanic and spanish fashion came after it. As Baroque period opened the French royal costume became magnificent and added Brition fashish to it. With the commencement of the modern ages the royal fashion came to an end. Modernages became peaple's period and the costume was simplified. After the First world wav designers and fashion books appeared with the development of technology. Thus the period of fashion industry came. For the designers in 20th country competed to create new designs, the fashion was changed year by year. The simplicity and practicality are not ignored in design, arid the designers added more atristic sense to dresses.

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Antimetastatic effect of several crude drugs with antitumor activity on B16-Fo and A549 cells ( I ) (항암활성 수종생약의 B16-Fo와 A549 암세포에 대한 항전이 효과( I ))

  • Kim, Sung-Hoon;Ryu, Shi-Yong
    • The Journal of Korean Medicine
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    • v.17 no.1 s.31
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    • pp.111-131
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    • 1996
  • For the development of antimetastatic agent 41 kinds of crude drugs were used for the evaluation of inhibitory effect of several crude drugs on cell adhesion of pulmonary cancer cells and platelet aggregation. Results were obtained as follows: 1. Water extracts of crude drugs inhibited cell adhesion of A549 to complex extracelluar matrix over 40 % of contol were Houttuyniae Herba, Mylabris, Rhei Radix et Rhizoma, Meliae Cortex, Ferula Resina, Oldenlandiae diffusae Herba at the higher concentration of $10^{-3}g/ml$ while those inhibiting cell adhesion of Bl6-Fo over 40 % of control were $10^{-5}g/ml$ of Houttuyniae Herba, Aurantii Fructus, Lithospermi Radix, Zedoariae Rhizoma. Prunellae Spica, Foeniculi Fructus, Rbei Radix, Scutellariae Radix, Meliae Cortex, Ferula Resina and Oldenlandiae diffusae Herba. 2. MeOH extracts of crude drugs at the concentration of $4{\times}10^{-4}g/ml$ inhibiting cell adhesion of A549 specifically to single extracelluar matrix over 40 % of control were Lithospermi Radix, Agrimoniae Herba, Rhei Radix and Ferula Resina to collagen I, Houttuyniae Herba, Lithospermi Radix, Bupleuri Radix, Salviae miltiorrhizae Radix, Orostachys Herba, Sappan Lignum, Meliae cortex ferula Resina and Coicis Semen to collagen Ⅳ, Mylabris, Agrimoniae Herba to laminin, Houttuyniae Herba and Meliae Cortex to fibronectin. 3. NeOH extracts of crude drugs at the concentration of $4{\times}10^{-4}g/ml$ inhibiting cell adhesion of B16-Fo specifically to single extracelluar matrix over 60 % of control were Lithospermi Radix, Salviae miltiorrhizae Radix, Meliae Cortex and Ferula Resina to collagen I, Lithospermi Radix, Bupleun Radix, Saiviae miltiorrhizae Radix, Ferula Resina and Acanthopanacis Cortex to collagen Ⅳ, Bupleuri Radix, Orostachys Herba to laminin, Houttuyniae Herba to fibronectin. 4. MeOH extracts of crude drugs inhibiting platelet aggregation over 40% of ADP control were at the concentration of $50{\mu}g/m{\ell}$ of Houttuyniae Herba, Angilicae gigantis Radix, Zedoariae Rhizoma. Coicis Semen and $100{\mu}g/m{\ell}$ of Ferula Resina, Orostachys Herba, Salviae miltiorrhizae Radix, Curcumac Radix, Carthami Flos, Lithospermi Radix, Gleditsiae Spina, Sappan Lignum, Acanthopanacis Cortex. These results suggest that several crude drugs including Ferula Resina, Houttuyniae Herba, Lithospermi Radix and Salviae miltiorrhizae Radix chiefly have more possibility to exert antimetastatic activity and require in vivo antimetastatic study.

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