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Gestational Exposure to Pesticides Induces Oxidative Stress and Lipid Peroxidation in Offspring that Persist at Adult Age in an Animal Model

  • Ndonwi, Elvis Ngwa;Atogho-Tiedeu, Barbara;Lontchi-Yimagou, Eric;Shinkafi, Tijjani S.;Nanfa, Dieudonne;Balti, Eric V.;Indusmita, Routray;Mahmood, Amena;Katte, Jean-Claude;Mbanya, Armand;Matsha, Tandi;Mbanya, Jean Claude;Shakir, Ali;Sobngwi, Eugene
    • Toxicological Research
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    • v.35 no.3
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    • pp.241-248
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    • 2019
  • Pesticide exposure may induce biochemical alterations including oxidative stress and lipid peroxidation. However, in the context of developmental origin of health and disease, putative trans-generational effect of exposure to pesticides are insufficiently studied. We therefore aimed to evaluate the biochemical effect of gestational exposure to four pesticides on female Wistar rats and their offspring at adult age. We studied 30 female nulliparous Wistar rats divided into 5 equal groups. Group 1 served as the control group and received distilled water while group 2, 3, 4 and 5 received orally pesticide 1 (imidacloprid), pesticide 2 (chlorpyrifos), pesticide 3 (imidacloprid + lambda cyhalothrin) and pesticide 4 (oxamyl) respectively once daily throughout gestation at a dose equivalent to 1/10 lethal dose 50. The mothers were followed up until one month post gestation. The offspring were followed up from birth until adult age (12 weeks). In all animals at each time point we evaluated malondialdehyde (MDA), oxidative stress and liver function enzymes. There was similar variation of total body weight in all the groups during and after gestation. However, Female Wistar rats of the exposed groups had significant alterations in liver SOD (-30.8% to +64.1%), catalase (-38.8% to -85.7%) and GSH (-29.2% to -86.5%) and; kidney catalase (> 100%), GSH (> 100%). Moreover, MDA, alanine transaminase (ALT) and aspartate transaminase (AST) levels were significantly higher in pesticide exposed rats compared to the control group. Similar alterations in antioxidant enzymes, MDA and liver function enzymes were observed in offspring of treated rats evidenced at weaning and persisting until adult age. Exposure to pesticides causes oxidative stress and lipid peroxidation in exposed female Wistar rats and their offspring. The persistence in offspring at adult age suggests transgenerational adverse effects.

Impact of Tofu Paste and Non-starch Polysaccharides on Oil Uptake Reduction in Cake Doughnuts (케이크 도넛의 흡유저감에 대한 두부 페이스트와 비전분성 탄수화물 고분자의 영향)

  • Jung, Gil-Young;Lee, Hyeon-Jeong;Ko, Eun-Sol;Kim, Hyun-Seok
    • Food Engineering Progress
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    • v.21 no.1
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    • pp.72-78
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    • 2017
  • The objective of this study was to investigate the effects of combinations of tofu paste and non-starch polysaccharides (NSP) on the oil uptake reduction (OTR) of deep-fat fried cake doughnuts. OTR agents were tofu paste (from grinding tofu with deionized water, followed by passage through a 60 mesh sieve), and five neutral and nine anionic NSPs. A control doughnut (without tofu paste or NSP), tofu doughnut (with tofu paste) and NSP-tofu doughnut (with tofu paste and NSP) were prepared. The moisture and total lipid (TL) content, cross-section image, color characteristic, and specific volume were measured. The tofu and NSP-tofu doughnuts exhibited higher moisture and lower TL content than the control. OTR was 10.8% for the tofu doughnut, and between 13.2% and 41.2% for the NSP-tofu doughnut. The highest OTR (41.2%) was found in the NSP-tofu doughnut with a combination of tofu paste and sodium alginate (NaA). The specific volume of the NSP-tofu doughnuts with combinations of tofu paste with NaA (2.5 mL/g), locust bean gum (2.5 mL/g), and ${\kappa}$-carrageenan (2.4 mL/g) was very close to that of the control (2.6 mL/g). Considering the OTR and specific volume of doughnuts, the combination of tofu paste and NaA would be most effective in reducing the oil uptake of doughnuts during deep-fat frying.

A Study on the Effect of Construction Safety and Health Management on the Post-management of Safety Inspection Evaluation (건설공사 안전 보건관리가 안전점검평가 사후관리에 미치는 영향관계)

  • Kim, Jin Tae;Shin, Yong Seung;Moon, Yu Mi
    • Journal of the Society of Disaster Information
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    • v.18 no.1
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    • pp.228-240
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    • 2022
  • A comprehensive safety management system will be required in accordance with the implementation of the Major Disaster Punishment Act for close-up safety management of construction sites. Safety management level evaluation management requires a comprehensive relationship between safety management under the Construction Technology Promotion Act and health and health management system under the Industrial Safety and Health Act. Purpose: Safety under the Serious Accidents Punishment Act. The ultimate goal is to study the comprehensive analysis and relationship of health management and to improve the safety evaluation level of health and health management. Methods: The feasibility of the questionnaire was confirmed through the second Delphi analysis of construction site experts and safety managers, and the regression coefficient and path analysis of potential variants in safety management, safety management, health management and safety inspection were confirmed. Result and Conclusion: In the structural model, the regression coefficient (89%) from safety management, health system, and safety management to safety inspection execution and lambda values of appropriate observation variables were confirmed. In the path analysis, the total effect (.809) was confirmed by mediating health hygiene in the relationship between health plan establishment adequacy and post-inspection management, and the path coefficient (.82) of temporary structure safety was confirmed.

Determination of mandipropamid residues in agricultural commodities using high-performance liquid chromatography with mass spectrometry (고성능액체크로마토그래피를 이용한 농산물 중 Mandipropamid의 잔류분석법 확립)

  • Kwon, Chan Hyeok;Chang, Moon Ik;Im, Moo Hyeog;Choi, Hoon;Jung, Da I;Lee, Su Chan;Yu, Jin Young;Lee, Young Deuk;Lee, Jong Ok;Hong, Moo Ki
    • Analytical Science and Technology
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    • v.21 no.6
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    • pp.518-525
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    • 2008
  • Mandipropamid is a new mandelamide-type fungicide to control foliar Oomycete pathogens in some vegetables. An analytical method was developed to determine mandipropamid residues in agricultural commodities using high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Mandipropamid was extracted with methanol from grape, tomato, green pepper, Chinese cabbage and potato samples. The extract was diluted with saturated sodium chloride solution and distilled water, and dichloromethane partition was followed to recover the mandipropamid from the aqueous phase. Florisil column chromatography was employed to further remove interfering co-extractives prior to HPLC analysis. Reverse-phased HPLC was successfully applied to determine mandipropamid in sample extracts with the detection at its ${\lambda}_{max}$ (223 nm). Overall recoveries of mandipropamid from fortified samples averaged $99.8{\pm}1.7$ (n=6), $89.3{\pm}5.3$ (n=6), $98.7{\pm}2.2$ (n=6), $99.7{\pm}6.8$ (n=6) and $91.1{\pm}3.1$ (n=6) for grape, tomato, green pepper, Chinese cabbage and potato, respectively. Limit of quantification of the method was 0.02~0.04 mg/kg for all samples. A LC/mass spectrometry with selected-ion monitoring was also provided to confirm the suspected residue. The proposed method was reproducible and sensitive enough to determine the terminal residue of mandipropamid in agricultural commodities.

The coping and defense features of the North Korean Defectors in the Rorschach test (로샤 검사에 나타난 북한이탈주민의 대처와 방어)

  • Yun Kyeung Choi;Jong Nam Kim;Jung-Min Chae
    • Korean Journal of Culture and Social Issue
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    • v.15 no.4
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    • pp.507-528
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    • 2009
  • Coping and defense are similar psychological mechanisms to reduce stress response, but they are regarded as distinct from each other. Defense involved distortions of reality, but coping was used for strategies associated with accepting the true nature of the situation. The purpose of the present study was to investigate the coping and defense features of the North Korean Defectors in the Rorschach test. The subjects were then categorized into four groups based on their birth place and gender: Male North Korean defectors( N=16), female North Korean defectors(N=28), male South Koreans(N=17), and female South Koreans(N=25). All subjects were individually administered the Rorschach test, and protocols were scored according to the Exner(2003)'s Rorschach Comprehensive systems. Coping or defense-related Rorschach variables were selected for analysis: EA, CDI, Adj D, EB style, Lamda, Zd, X-%, Xu%, W:Dd:D, P, reaction time, and other behavior characteristics during the testing. Compared with other groups, female North Korean defectors were more likely to show poor coping resources(low EA), adjustment difficulties(Adj D), and distortions of reality(high X-%). They also demonstrated more 'don't know' responses and more delayed responses than other groups. These findings suggest that poor coping resources make female North Korean defectors more vulnerable to stress, and psychological intervention such as social skills training may help them settle successfully in south Korea. Limitations of the current study and implications for future research were discussed.

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Performance Analysis of Receiver for Underwater Acoustic Communications Using Acquisition Data in Shallow Water (천해역 취득 데이터를 이용한 수중음향통신 수신기 성능분석)

  • Kim, Seung-Geun;Kim, Sea-Moon;Yun, Chang-Ho;Lim, Young-Kon
    • The Journal of the Acoustical Society of Korea
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    • v.29 no.5
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    • pp.303-313
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    • 2010
  • This paper describes an acoustic communication receiver structure, which is designed for QPSK (Quadrature Phase Shift Keying) signal with 25 kHz carrier frequency and 5 kHz symbol rate, and takes samples from received signal at 100 kHz sampling rate. Based on the described receiver structure, optimum design parameters, such as number of taps of FF (Feed-Forward) and FB (Feed-Back) filters and forgetting factor of RLS (Recursive Least-Square) algorithm, of joint equalizer are determined to minimize the BER (Bit Error Rate) performance of the joint equalizer output symbols when the acquisition data in shallow water using implemented acoustic transducers is decimated at a rate of 2:1 and then enforced to the input of receiver. The transmission distances are 1.4 km, 2.9 km, and 4.7 km. Analysis results show that the optimum number of taps of FF and FB filters are different according to the distance between source and destination, but the optimum or near optimum value of forgetting factor is 0.997. Therefore, we can reach a conclusion that the proper receiver structure could change the number of taps of FF and FB filters with the fixed forgetting factor 0.997 according to the transmission distance. Another analysis result is that there are an acceptable performance degradation when the 16-tap-length simple filter is used as a low-pass filter of receiver instead of 161-tap-length matched filter.

Luminescence properties of $(Y,\;Zn)_2O_3$:$Eu^{3+}$ red phosphor as the effect of Zn ion (Zn ion의 영향에 따른 $(Y,\;Zn)_2O_3$:$Eu^{3+}$ 적색 형광체의 발광특성)

  • Song, Y.H.;Moon, J.W.;Park, W.J.;Yoon, D.H.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.18 no.6
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    • pp.253-257
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    • 2008
  • To enhance the luminescence properties, the red phosphor composed of $(Y,\;Zn)_2O_3$:$Eu^{3+}$ as doping concentration of Zn ion is synthesized at $1200^{\circ}C$ for 6 hrs in air atmosphere by conventional solid reaction method. As a result of the red phosphor $(Y,\;Zn)_2O_3$:$Eu^{3+}$ is measured X-ray diffraction (XRD), The main peak is nearly corresponded to the same as JCPDS card (No. 41-1105). When the doping concentration of Zn ion is more than 5 mol%, However, the ZnO peak is showed by XRD analysis. Therefore, when the doping concentration of Zn ion is less than 5 mol%, the Zn ion is well mixed in $Y_2O_3$ structure without the impurity phases. The photoluminescence (PL) properties is shown as this phosphor is excited in 254 nm region and the highest emission spectra of $(Y,\;Zn)_2O_3$:$Eu^{3+}$ has shown in 612 nm region because of a typical energy transition ($^5D_0{\rightarrow}^7F_2$) of $Eu^{3+}$ ion. As the doping concentration of Zn ion is more than 10 mol%, the emission peak is suddenly decreased. when the highest emission peak as doping concentration of Zn ion is shown, the composition of this phosphor is $(Y_{0.95},\;Zn_{0.05})_2O_3$:$Eu^{3+}_{0.075}$ and the particle size analyzed by FE-SEM is confirmed from 0.4 to $3{\mu}m$.

Performance Evaluation of WWTP Based on Reliability Concept (신뢰성에 기초한 하수처리장 운전효율 평가)

  • Lee, Doo-Jin;Sun, Sang-Woon
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.3
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    • pp.348-356
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    • 2007
  • Statistical and probabilistic method was used in the analysis of data, which is the most effective one in describing the various natures, and the methodology relating the results with the design was developed. Influents and effluents of three treatment plants were analyzed and the focus was made on BOD, COD, SS, IN, TP The fluctuations of influent such as BOD, COD, SS were extremely large and their standard deviations(st.dev) were more than 10 mg/L. but those of TN, TP were small; the st.dev was 6.6 mg/L for TN, 0.6 mg/L for TP, respectively. But, effluent concentration showed consistent pattern regardless of the influent fluctuations, the st.dev was ranged between 0.28 and 4.48 mg/L. Effluent distributional characteristics were as follows; BOD, COD were distributed normally, but SS, TN, and TP, log-normally; unsymmetric and skewed to the right. The coefficient of reliability(COR) based on the results of statistics of data was introduced to evaluate the process performance an4 to reflect the process performance to the process design. The coefficient of reliability relates the design value(the goal) with the standards and it can be used in operating treatment facilities under a certain reliability level and/or in evaluating the reliability of the treatment facilities on operation. Each treated water quality of effluent showed the half of water quality standards in the level of 50% percentile and all treatment plant was achieved 100% probability of water quality standards. It was concluded that the variability of the process performance should be reflected to the design procedure and the standards through the analysis based on the statistics and the probability.

Ecological Studies on Vegetation Recovery of Burned Field after Forest Fire (산화적지의 식생회복에 관한 생태학적 연구)

  • Kang, Sang Joon;Jong Tai Lee
    • Journal of environmental and Sanitary engineering
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    • v.5 no.1
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    • pp.54-62
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    • 1990
  • The recovery of vegetatiion and soil properties in the burned fields after forest fire were studied in Chung Cheong Buk Do area, Korea, from July 23 to 28, 1981. Miscanthus sinensis var. purpurascens, Carex humilis and Lespedeza bicolor were dominant species in the burned field of the second year after forest fire as well as unburned field. Especially, Lespedeza bicolor was gradually grown to the dominant species. Lespedeza bicolor, Carex humilis and Miscanthus sinensis var. purpurascens were the dominant species in the burned field of the fifth year after forest fire. Biological spectra of the burned fields were similar to the umburned fields with $H-e-D_1-R_5$ or $Ph-e-D_1-R_5$ from the second year after forest fire. Accordingly, biological spectra were recovered to the unburned fields from the second year. Degree of successiion was DS=423 in the burned field and DS=524 in the unburned field in 1981. The DS of the burned fields was gradually increased and recovered to be similar to the unburned from the second year. In the species diversities and evenness index, H,e and $\beta$ of the burned field in 1981 were higher and $\lambda$ was lower than the unburned field, but all of the indices were recovered to the unburned field from the second or third years. Accordingly, the vegetation of the first year was the complex community in view floristic composition, but it was recovered to the simple community as unburned field fromthe second or third years. In the soil preperties, pH, total nitrogen, available phosphorus, exchangeable potassium, exchangeable calcium and exchangeable magnesium were increased and organic matter was decreased due to forest fire, and then was recovered to the unburned field from the second or third years. The vegetation and soil properties of the burned field after forest fire were similary recovered to the unburned field from the second or third years. Accordingly, there was a close relationship between the trend of vegetation recovery and the changes of soil characteristics after forest fire.

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Degradation of Microcystin-LR, Taste and Odor, and Natural Organic Matter by UV-LED Based Advanced Oxidation Processes in Synthetic and Natural Water Source (UV-LED기반 고도산화공정을 이용한 수중 마이크로시스틴-LR, 이취미 물질, 자연유기물 분해)

  • Yang, Boram;Park, Jeong-Ann;Nam, Hye-Lim;Jung, Sung-Mok;Choi, Jae-Woo;Park, Hee-Deung;Lee, Sang-Hyup
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.5
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    • pp.246-254
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    • 2017
  • Microcystin-LR (MC-LR) is one of most abundant microcystins, and is derived from blue-green algae bloom. Advanced oxidation processes (AOPs) are effective process when high concentrations of MC-LR are released into a drinking water treatment system from surface water. In particular, UV-based AOPs such as UV, $UV/H_2O_2$, $UV/O_3$ and $UV/TiO_2$ have been studied for the removal of MC-LR. In this study, UV-LED was applied for the degradation of MC-LR because UV lamps have demonstrated some weaknesses, such as frequent replacements; that generate mercury waste and high heat loss. Degradation efficiencies of the MC-LR (initial conc. = $100{\mu}g/L$) were 30% and 95.9% using LED-L (280 nm, $0.024mW/cm^2$) and LED-H (280 nm, $2.18mW/cm^2$), respectively. Aromatic compounds of natural organic matter changed to aliphatic compounds under the LED-H irradiation by LC-OCD analysis. For application to raw water, the Nak-dong River was sampled during summer when blue-green algae were heavy bloom in 2016. The concentration of extracellular and total MC-LR, geosmin and 2-MIB slightly decreased by increasing the LED-L irradiation; however, the removal of MC-LR by UV-LED (${\lambda}=280nm$) was insufficient. Thus, advanced UV-LED technology or the addition of oxidants with UV-LED is required to obtain better degradation efficiency of MC-LR.