• 제목/요약/키워드: $^{40}K$

검색결과 43,030건 처리시간 0.096초

CD40L 발현 K562 세포주를 이용한 시험관내 B 세포 증식과 항원제공능 증가 (Enhancement of Proliferation and Antigen Presentation of Human B Cells in Vitro by K562 Cells Expressing CD40L)

  • 박정용;윤성희;김은경;윤선옥;손현정;김태규
    • IMMUNE NETWORK
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    • 제7권2호
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    • pp.80-86
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    • 2007
  • Background: CD40-activated B (CD40-B) cells might be an attractive source of autologous antigen-presenting cells (APCs) for immunotherapy due to the convenience to obtain from peripheral blood and expand in vitro. Moreover, CD40-B cells were found to be comparable with DCs in their capacity to raise antigen-specific CD8+ T cells. Here, we have established K562 cells expressing CD40L to expand CD40-activated B cells used for APCs. Methods: After activation of B cell by K562/CD40L, CD40-B cells were examined by counting B cell numbers. Surface expression of CD54, CD80, CD86 and HLA class II was measured by flow cytometry. The CD40-B cells were tested for its function as APC by mixed lymphocyte reactions (MLR) and by induction of T cell responses specific for pp65 peptide in vitro. Results: The expansion of B cells by K562/CD40L increased about 6-folds compared with anti-CD40 or K562. Furthermore, the expression of CD54, CD80, CD86 and HLA class II was up-regulated by K562/CD40L. B cells by K562/CD40L showed comparable antigen presentation activity with mature DCs as shown in MLR, INF-${\gamma}$ ELISPOT assay. Conclusion: These results suggest that K562/CD40L could be used to generate activated B cells as potent APCs which could be useful for cellular vaccination and adoptive immunotherapy.

40 kHz 초음파에 의해 유도된 효모세포 모델에서 세포사멸 패턴 (A pattern of cell death induced by 40 kHz ultrasound in yeast cell model)

  • 김지욱;공희정;김영환;강광일
    • 한국음향학회지
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    • 제36권3호
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    • pp.172-178
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    • 2017
  • 초음파는 세포사멸을 포함하여 의학 및 생물학분야에 널리 응용되고 있으나 그 정확한 기작에 대해선 논쟁의 여지가 있다. 본 연구에서는 40 kH 초음파 조사시스템을 단세포 효모에 적합하게 개발하고 세포사멸 유도시 40 kH 초음파의 생물학적 현상을 살펴보았다. 아이오딘화 칼륨 선량 측정법을 이용하여 1.5 ml 실험튜브에 40 kH 초음파 조사 시스템의 최적 조건을 맞추어 세포사멸을 시간 의존적 방식으로 연구하였고 초음파 조사과정동안 온열효과와는 별개로 세포 사멸이 관찰되었다. 40 kH 초음파와 과산화수소의 동시 처리는 세포사멸에 상조적인 효과가 관찰되어 활성산소가 40 kH 초음파사멸에 관련이 있었다. 그러나 활성산소 저해제, NAC(N-acetyl-Lcysteine)는 초음파에 의한 세포사멸에 약한 영향만을 미쳤고 다른 세포사멸, 괴사억제제[글리실리진(glycyrrhizin) 또는 zVAD-fmk] 역시도 세포사멸을 완전히 억제하진 못하였다. 본 연구를 통하여 40 kH 초음파에 의한 세포사멸에는 온열효과나 활성산소만으로 사멸이 유도되지는 않는 것으로 보인다.

Differential Gene Expression in GPR40-Overexpressing Pancreatic ${\beta}$-cells Treated with Linoleic Acid

  • Kim, In-Su;Yang, So-Young;Han, Joo-Hui;Jung, Sang-Hyuk;Park, Hyun-Soo;Myung, Chang-Seon
    • The Korean Journal of Physiology and Pharmacology
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    • 제19권2호
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    • pp.141-149
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    • 2015
  • "G protein-coupled receptor 40" (GPR40), a receptor for long-chain fatty acids, mediates the stimulation of glucose-induced insulin secretion. We examined the profiles of differential gene expression in GPR40-activated cells treated with linoleic acid, and finally predicted the integral pathways of the cellular mechanism of GPR40-mediated insulinotropic effects. After constructing a GPR40-overexpressing stable cell line (RIN-40) from the rat pancreatic ${\beta}$-cell line RIN-5f, we determined the gene expression profiles of RIN-5f and RIN-40. In total, 1004 genes, the expression of which was altered at least twofold, were selected in RIN-5f versus RIN-40. Moreover, the differential genetic profiles were investigated in RIN-40 cells treated with $30{\mu}M$ linoleic acid, which resulted in selection of 93 genes in RIN-40 versus RIN-40 treated with linoleic acid. Based on the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG, http://www.genome.jp/kegg/), sets of genes induced differentially by treatment with linoleic acid in RIN-40 cells were found to be related to mitogen-activated protein (MAP) kinase- and neuroactive ligand-receptor interaction pathways. A gene ontology (GO) study revealed that more than 30% of the genes were associated with signal transduction and cell proliferation. Thus, this study elucidated a gene expression pattern relevant to the signal pathways that are regulated by GPR40 activation during the acute period. Together, these findings increase our mechanistic understanding of endogenous molecules associated with GPR40 function, and provide information useful for identification of a target for the management of type 2 diabetes mellitus.

Genetic Trends for Laying Traits in the Brown Tsaiya (Anas platyrhynchos) Selected with Restricted Genetic Selection Index

  • Chen, D.T.;Lee, S.R.;Hu, Y.H.;Huang, C.C.;Cheng, Y.S.;Tai, C.;Poivey, J.P.;Rouvier, R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권12호
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    • pp.1705-1710
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    • 2003
  • A small body size of Brown Tsaiya laying duck is desirable to reduce maintenance requirements, so the body weight at 40 weeks of age (BW40) has to be maintained at its current level. Egg weight has to be maintained at around 65 g to meet market requirements. Eggshell strength at 40 weeks of age (ES40) must to be increased in order to maintain a low incidence of broken eggs. Thus, number of eggs laid up to 52 weeks of age (EN52) has to be increased without negative correlated response on ES40. A new linear genetic selection index was used: $I_g=a_0{\times}GEW40\;(g)+a_1{\times}GBW40\;(g)+a_2{\times}GES40\;(kg/cm^2)+a_3{\times}GEN52\;(eggs)$ where GEW40, GBW40, GES40 and GEN52 were the multitrait best linear unbiased prediction (MT-BLUP) animal model predictors of the breeding values respectively of egg weight and body weight at 40 weeks of age (EW40, BW40), ES40 and EN52. The coefficients $a_0$, $a_1$, $a_2$ and $a_3$ were calculated with constraints of 0.0 g, 0.0 g and $0.013kg/cm^2$ for expected genetic gains in EW40, BW40 and ES40 respectively and maximum gain in EN52. Since 1997, the drakes and the ducks were selected according to their own indexes, with this new genetic selection index. From G0 to G4, the average per generation predicted genetic responses in female duck were +0.05 g for EW40, +0.92 g for BW40, $+0.035kg/cm^2$ for ES40 and +2.13 eggs for EN52. Which represented respectively 0.07%, 0.06%, 0.67% and 1.0% of the means of the EW40, BW40, ES40 and EN52. For ES40 and EN52, it represented also respectively 16.1% and 21.6% of the additive genetic standard deviation of these traits. Thevse results indicated that selection of laying Brown Tsaiya by a restricted genetic selection index and with MT-BLUP animal model could be an efficient tool for improving the efficiency of egg production, increasing egg shell strength and egg number while holding egg weight and body weight constants.

Validity and reliability of the Korean version of the Quality of Recovery-40 questionnaire

  • Lee, Jun Ho;Kim, Deokkyu;Seo, Donghak;Son, Ji-seon;Kim, Dong-Chan
    • Korean Journal of Anesthesiology
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    • 제71권6호
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    • pp.467-475
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    • 2018
  • Background: The Quality of Recovery-40 (QoR-40) is a widely-used, self-rated, and self-completed questionnaire for postoperative patients. The questionnaire is intended to elicit information from each patient regarding the quality of recovery during the postoperative period. It is noteworthy, however, that an official Korean version of the QoR-40 (QoR-40K) has not been established. The purpose of this study was to develop the QoR-40K by translation and cultural adaptation process and to evaluate the validity and reliability of the QoR-40K. Methods: After pre-authorization from the original author of the QoR-40, the translation procedure was established and carried out based upon Beaton's recommendation to create a QoR-40K model comparable to the original English QoR-40. Two hundred surgical patients were enrolled, and each completed the questionnaire during the preoperative period, on the third day, and 1 month after surgery. The QoR-40K was compared with the visual analogue scale (VAS) and another health-related questionnaire, the Short-form Health Survery-36 (SF-36). The method of validation for QoR-40K included test-retest reliability, internal consistency, and level of responsiveness. Results: Spearman's correlation coefficient for test-retest reliability was 0.895 (P < 0.001), and Cronbach's alpha of the global QoR-40K on the third day after surgery was 0.956. A positive correlation was obtained between the QoR-40K and the mental component summary of SF-36 (${\rho}=0.474$, P < 0.001), and a negative correlation was observed between QoR-40K and VAS (${\rho}=-0.341$, P < 0.001). The standardized responsive mean of the total QoR-40K was 0.71. Conclusions: The QoR-40K was found to be as acceptable and reliable as the original English QoR-40 for Korean patients after surgery, despite the apparent differences in the respective patients' cultural backgrounds.

한국인간공학 40년 성과와 과제 그리고 미래 40년의 전망 (40 Years of Ergonomics in Korea : Accomplishments, Challenges and 40 More Years Ahead)

  • 정민근;윤명환;박재희;이인석;임지현
    • 대한산업공학회지
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    • 제40권6호
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    • pp.568-579
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    • 2014
  • For the commemoration of 40 years of Industrial Engineering in Korea, Ergonomics in Korea for the last 40 years are presented in this paper. Historical backgrounds, past achievements, and key historical landmarks are explained and analyzed. In addition, a keyword content analyses on the 1,005 titles from the research paper published from 1983 to 2014 are conducted to display the structural patterns of the changes of research focus of the ergonomics in Korea. Finally, some important perspectives with regard to the prospects for the future of ergonomics in Korea are also summarized.

재배년수에 따른 삼백초 재식거리별 생산성 변화 (Change of Productivity According to Cultivation Years and Planting densities in Saururus chinensis Baill)

  • 남상영;김인재;김민자;윤태;이철희;박성규;이우영;정승근
    • 한국약용작물학회지
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    • 제14권3호
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    • pp.134-138
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    • 2006
  • 재배년수 및 재식거리에 따른 삼백초의 생산성을 구명하여 재배법 확립의 기초자료를 제공하고자 $40{\times}5\;cm$ 등 6처리를 두어 2002년부터 3년간 시험한 결과를 요약하면 다음과 같다. 가. 경엽 생육중 초장은 1년생은 소식할수록 좋았으나, 2년생은 재식거리 $40{\times}10\;cm$, 3년생은 $40{\times}20\;cm$에서 가장 좋았으며, 분얼수는 1, 2, 3년생 모두 밀식할수록 많았고, 근경생육은 1, 2년생은 밀식할수록 양호한 경향이었으나, 3년생은 $40{\times}20\;cm$에서 가장 좋았다. 나. 상품 경엽수량은 1년생은 재식거리 $40{\times}5\;cm$, 2년생 $40{\times}10\;cm$, 3년생 $40{\times}20\;cm$, $1{\sim}2$년 누적수량 $40{\times}10\;cm$, $1{\sim}3$년 누적수량 $40{\times}10$, 20 cm에서 가장 많았다.

불소/실리콘 고무 블렌드의 특성과 물성 (Characteristics and Properties of Fluoro/Silicone Rubber Blend System)

  • 이진국;송환제;김미라
    • 접착 및 계면
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    • 제9권3호
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    • pp.14-19
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    • 2008
  • 본 연구는 Silicone rubber (SR40)과 Fluororubber (FKM)을 여러 가지 무게비로 하여 블렌드를 제조하였고 그 특성을 고찰하였다. 블렌드의 가교 거동은 SR40의 함량이 증가함에 따라 SR40 내의 가교제에 의해 가교 속도는 빨라졌다. 블렌드에서 FKM의 함량이 증가하면, 열분해 온도가 증가는 경향이 있고 SR40/FKM 블렌드 비율이 50/50일 때 순수한 SR40보다 높은 열안정성을 나타내었다. 표면 발수성의 척도인 접촉각을 측정한 결과, SR40의 함량이 증가할수록 블렌드의 접촉각은 증가하는 것을 확인하였다. 또한, 제조한 블렌드들은 모든 조성에서 전형적인 상분리의 모폴로지를 나타내는 것을 확인할 수 있었고, SR40의 비율이 증가할수록 상분리 정도가 증가하는 것을 확인할 수 있었다.

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한국토양(韓國土壤)의 방사능(放射能)에 관(關)한 연구(硏究) (I) - 답토양(畓土壤)의 β 방사능(放射能) 및 40K 핵종동정법(核種同定法) - (Radioactivity in soils (I) -A method for the identification of 40K and measurement of beta activity in paddy soils)

  • 김태순
    • 한국토양비료학회지
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    • 제4권1호
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    • pp.55-66
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    • 1971
  • 가. 토양(土壤)의 전(全) ${\beta}$ 방사능(放射能)을 측정(測定)하여 전(全) ${\beta}$ 방사능(放射能) 중(中)에서 $^{40}K$의 기여(奇與)를 $^{40}K$${\beta}$ 선(線) 포화(飽和) 방사능비율(放射能比率)을 사용(使用)하여 전(全) ${\beta}$ 선(線) 분석(分析)을 시도(試圖)한 결과(結果) 답토양(畓土壤)의 전(全) ${\beta}$ 방사능(放射能)은 $^{40}K$${\beta}$ 방사능(放射能)에 기곤(起困)함을 알았다. 따라서 답(畓) 토양(土壤)의 전(全) ${\beta}$ 방사능(放射能)을 측정(測定)한다는 것은 실(實)은 $^{40}$${\beta}$ 방사능(放射能)을 측정(測定)하는 것이다. 나. 전(全) ${\beta}$ 방사능(放射能)으로 부터 $^{40}K$의 핵종(核種) 동정(同定)을 행(行)하는 순서(順序)는 임의시료량(任意試料量)의 방사능측정(放射能測定)와 $^{40}K$의 포화(飽和) 방사능(放射能) 비율(比率)로 계산(計算)한 계산치(計算値)((4)식(式)에서 $G(t){\cdot}A_{\infty}$ 또는 A/G(t))와 비교(比較)하여 일치(一致)되는지를 Chauvenet 기준(基準) 따라서 검정(檢定)한다. 만약에 일치(一致)하지 않을 경우에는 $^{40}K$ 외(外)의 다른 핵종(核種)의 기여(寄與)를 의미(意味)하며 (7)식(式)에 의(依)하여 $^{40}K$과 다른 핵종(核種)을 분별(分別)하여 각각의 방사능(放射能) 붕양율(崩壤率)을 구(求)할 수 있다. 다. 한국(韓國) 답(畓) 토양(土壤)의 $^{40}K$ ${\beta}$ 방사능(放射能)은 $17.5{\sim}44.6{\mu}{\mu}ci/gr$. soil 사이에 있으며 이를 다시 모암(母岩) 별(別)로 보면 석회암(石灰岩)>화강암(花崗岩)>편암(片岩)>편마암(片麻岩)>반암(斑岩)>현무암(玄武岩)>섬녹암(閃綠岩)의 순(順)이다.

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40mm 저속유탄(K200) 신관 품질개선을 통한 불발율 감소에 관한 연구 (Research on the Decrease of Dud Ammunition Rate of 40mm Grenade(K200) Fuze through Quality Improvement)

  • 주진천;김용화;안남수;김상민;하수라
    • 한국산학기술학회논문지
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    • 제17권6호
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    • pp.700-707
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    • 2016
  • 최근 군에서 사격 훈련 중 1990년에 제조된 40mm 저속유탄(K200)은 탄착불발에 의한 탄약 악작용이 발생하였다. 악작용 내용은 총 72발의 사격 중 11발의 불발탄이 발생되어 불발율은 15% 정도였다. 탄약은 장기 저장 후 사용하는 품질특성을 가지고 있으므로 장기 저장시에도 성능 및 안전성 등의 확보가 필수적이다. 따라서 본 연구에서는 40mm 저속유탄용 신관의 품질개선을 통해 불발율을 최소화하고자 하였다. 첫 번째로 기폭관 성능시험, 발사시험 등을 수행한 결과 불발의 원인을 신관 화공품인 기폭관의 경시변화에 의한 성능저하로 판단하였다. 두 번째로 불발율을 최소화하기 위해 기폭관의 화약량을 증가시키고 화약량 증가에 따른 부품의 치수를 변경하는 등 40mm 저속유탄 신관의 품질개선을 추진하였다. 마지막으로 품질 개선 전 후 기존품과 개선품의 가속노화시험을 통해 저장수명을 예측해 본 결과 기존품은 6.5년, 개선품은 45.5년으로 개선되어 신관의 수명이 약 7배 향상된 결과를 도출하였다. 이에 품질 개선된 40mm 저속유탄 신관은 장기 저장시 불발율이 감소되어 40mm 저속유탄의 안전성, 신뢰성, 성능 향상에 기여할 것으로 판단된다.