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Enhancement of Proliferation and Antigen Presentation of Human B Cells in Vitro by K562 Cells Expressing CD40L  

Park, Jung-Yong (Department of Microbiology, College of Medicine, The Catholic University of Korea)
Yoon, Sung-Hee (Department of Microbiology, College of Medicine, The Catholic University of Korea)
Kim, Eun-Kyung (Department of Microbiology, College of Medicine, The Catholic University of Korea)
Yun, Sun-Ok (Department of Microbiology, College of Medicine, The Catholic University of Korea)
Sohn, Hyun-Jung (Catholic Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of Korea)
Kim, Tai-Gyu (Department of Microbiology, College of Medicine, The Catholic University of Korea)
Publication Information
IMMUNE NETWORK / v.7, no.2, 2007 , pp. 80-86 More about this Journal
Abstract
Background: CD40-activated B (CD40-B) cells might be an attractive source of autologous antigen-presenting cells (APCs) for immunotherapy due to the convenience to obtain from peripheral blood and expand in vitro. Moreover, CD40-B cells were found to be comparable with DCs in their capacity to raise antigen-specific CD8+ T cells. Here, we have established K562 cells expressing CD40L to expand CD40-activated B cells used for APCs. Methods: After activation of B cell by K562/CD40L, CD40-B cells were examined by counting B cell numbers. Surface expression of CD54, CD80, CD86 and HLA class II was measured by flow cytometry. The CD40-B cells were tested for its function as APC by mixed lymphocyte reactions (MLR) and by induction of T cell responses specific for pp65 peptide in vitro. Results: The expansion of B cells by K562/CD40L increased about 6-folds compared with anti-CD40 or K562. Furthermore, the expression of CD54, CD80, CD86 and HLA class II was up-regulated by K562/CD40L. B cells by K562/CD40L showed comparable antigen presentation activity with mature DCs as shown in MLR, INF-${\gamma}$ ELISPOT assay. Conclusion: These results suggest that K562/CD40L could be used to generate activated B cells as potent APCs which could be useful for cellular vaccination and adoptive immunotherapy.
Keywords
Antigen-presenting cell; B cell; CD40L; K562; immunotherapy;
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