• Journal of Korean Neurosurgical Society
• /
• v.48 no.1
• /
• pp.14-19
• /
• 2010

Objective : Allelic losses or loss of heterozygosity (LOH) at many chromosomal loci have been found in the cells of meningiomas. The objective of this study was to evaluate LOH at several loci of different chromosomes (1p32, 17p13, 7q21, 7q31, and 22q13) in different grades of meningiomas. Methods : Forty surgical specimens were obtained and classified as benign, atypical, and anaplastic meningiomas. After DNA extraction, ten polymorphic microsatellite markers were used to detect LOH. Medical and surgical records, as well as pathologic findings, were reviewed retrospectively. Results : LOH at 1p32 was detected in 24%, 60%, and 60% in benign, atypical, and anaplastic meningiomas, respectively. Whereas LOH at 7q21 was found in only one atypical meningioma. LOH at 7q31 was found in one benign meningioma and one atypical meningioma. LOH at 17p13 was detected in 4%, 40%, and 80% in benign, atypical, and anaplastic meningiomas, respectively. LOH at 22q13 was seen in 48%, 60%, and 60% in benign, atypical, and anaplastic meningiomas, respectively. LOH results at 1p32 and 17p13 showed statistically significant differences between benign and non-benign meningiomas. Conclusion : LOH at 1p32 and 17p13 showed a strong correlation with tumor progression. On the other hand, LOH at 7q21 and 7q31 may not contribute to the development of the meningiomas.

• Journal of Aquaculture
• /
• v.21 no.1
• /
• pp.41-46
• /
• 2008

The purpose of the study was to suggest the optimal lipid enrichment conditions used digestive enzyme activity of rotifer changing due to water temperature and salinity. The high population growth appeared at the experiment temperature more than 28 degrees highly on the culture temperature(maximum 32 degrees, 1,453 individual/mL). The fecundity was low at high temperature, and the egg ratio was high at low temperature. Population growth of 10 and 15 ppt appeared in most highly, but the fecundity and the egg ratio were high most significantly appeared in natural seawater(32 psu). The digestive enzyme activity by the culture environment mainly showed high activity in natural seawater(amylase exclusion, 15 psu). However, the TAP activity by the water temperature showed highly at the more high temperature, but the amylase and the lipase appeared at low temperature. We carried out the lipid enrichment at 20 degrees and 26 degrees in a condition of the natural seawater. Total protein, the total essential amino acids differed not significantly. The methionine content that was essential amino acids, a total lipid content, unsaturated index of fatty acids, DHA and the DHA/EPA ratio were high significantly each in $20^{\circ}C$ enrichment trial. Therefore, we could suggest the $20^{\circ}C$ and natural seawater for the optimal lipid enrichment condition in aquaculture, because methionine contents, several indexes by the lipid, TG-lipase activity, fecundity and egg ratio are high.

• The korean journal of orthodontics
• /
• v.30 no.1 s.78
• /
• pp.101-114
• /
• 2000

The purpose of this study was to evaluate the soft-tissue profile changes from 24 to 32 yews of age in Korean adults. The subjects used in this study consisted of 17 males and 8 females. The data obtained from the lateral cephalograms taken at age 24 and 32, were analyzed statistically. The obtained results were as follows : 1. During the observation period, there were no significant changes in the hard-tissue measurements in both sexes (p>0.05). 2. In the anteroposterior measurements of soft-tissue, the males had significant increase in chin area (Pog', Gn'), and the females had significant decrease in lower lip (LI) (p<0.05). 3. In the vertical measurements of soft-tissue, both sexes showed the increased tendency in upper lip length (Sn-Sto) where the males showed significant increase (p<0.05). The lower facial height (Sn-Gn') turned out significant increase in both sexes (p<0.05). 4. In general, the females and males had the increased tendency in soft-tissue thickness, especially significant increase in chin area (Pog', Gn') (p<0.05).

• Journal of Genetic Medicine
• /
• v.1 no.1
• /
• pp.45-50
• /
• 1997

Neuroblastoma, a pediatric malignant neoplasm of neural crest origin, has a wide range of clinical virulence. The mechanisms contributing to the development of neuroblastomas are largely unclear, but non-random chromosomal changes identified over the past years suggest the involvement of genetic alterations. Amplification of the human N-myc proto-oncogene is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions (HSRs) of aggressively growing neuroblastomas. N-myc maps to chromosome 2 band 24, but HSR have never been observed at this band, suggesting transposition of N-myc during amplification. We have constructed and analyzed the region-specific painting probe for HSR in neuroblastoma IMR-32 to determine the derivative chromosomes. Microdissection was performed on HSR using an inverted microscope with the help of microglass needles and an micromanipulator. We pretreated the microdissected fragments with Topoisomerase I which catalyzes the relaxation of supercolled DNA, and performed two initial rounds of DNA synthesis with T7 DNA polymerase followed by conventional PCR to enable the reliable preparation of Fluorescent in situ hybridization probe from a single microdissected chromosome. With this method, it was possible to construct the region-specific painting probe for HSR. The probe hybridized specifically to the HSRs of IMR-32, and to 2p24, 2p13 of normal chromosome. Our results suggest there was coamplification of N-myc together with DNA of the chromosome 2p24 and 2p13. Moreover, the fluorescent signals for the amplified chromosomal regions in IMR-32 cells were also easily recognized at a Thus this painting probe can be applied to detect the similar amplification of N-myc in neuroblastoma tissue, and the probe pool for HSR may be used to identify the cancer-relevant genes.

• Korean Journal of Food Science and Technology
• /
• v.51 no.4
• /
• pp.343-347
• /
• 2019

This study was performed to develop an economical culture method of lactic acid bacteria (LAB) for kimchi fermentation. Leuconostoc mesenteroides WiKim32 was grown to $1{\times}10^9CFU/mL$ and maintained at 8.88 log CFU/mL for four days by culturing in kimchi cabbage juice (KCJ) without supplements and sterilization. Leuconostoc mesenteroides WiKim32 was cultured in 100 mL of KCJ by inoculating with 0.1% starter culture and adding 100 mL of KCJ everyday by adjusting pH to 5.5 using 1 M NaOH at $20^{\circ}C$ for four days. KCJ was prepared by extraction of kimchi cabbage leaves after washing them with citric acid and ethanol. Adjusting pH over 6.0 was favorable for the growth of LAB in the initial stage, but LAB growth was retarded in the later stage. In contrast, adjusting pH below 5.0 was not beneficial for the growth of LAB; therefore, pH was adjusted to 5.5.

• Journal of radiological science and technology
• /
• v.6 no.1
• /
• pp.116-116
• /
• 1983

• Bulletin of the Korean Chemical Society
• /
• v.32 no.8
• /
• pp.2776-2778
• /
• 2011

• Bulletin of the Korean Chemical Society
• /
• v.32 no.8
• /
• pp.2791-2794
• /
• 2011

• 대한핵의학회:학술대회논문집
• /
• 1966.11a
• /
• pp.93.2-93.2
• /
• 1966

• Journal of the Chungcheong Mathematical Society
• /
• v.32 no.1
• /
• pp.97-111
• /
• 2019

The purpose of work is to study the sums of k distanced numbers in Pell sequence {$P_i$} as well as in the generalized ${\lambda}-Pell$ sequence {$P_{{\lambda},i}$}.