• Title/Summary/Keyword: $^{32}P$

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Studies on the Distribution of Radiophosphorus(32P) in Pregnant Rats (임신 Rat에 있어서 방사성인(32P)의 흡수 분포에 관한 연구)

  • Lee, Kang Wook;Chung, Yung Chai;Lee, Heung Shik;Nam, Tchi Chou
    • Korean Journal of Veterinary Research
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    • v.9 no.2
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    • pp.49-53
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    • 1969
  • The distribution of radiophosphorus ($^{32}P$) was observed in three groups of female Albino tat consisted of early pregnancy group (10 days of pregnancy), late pregnancy group (18 days of pregnancy) and nonpregnanted control group. The results obtained were as follows: 1. The various sites arranged in the descending of absorption rate of $^{32}P$ were femur, mandible, liver, spleen, ovary, fetus (only from early pregnancy group) and kidney in both groups of non-pregnanted and early pregnancy and femur, mandible, fetus, liver, ovary, spleen and kidney in the late pregnancy group. 2. The significant difference (P<0.05) in the absorption rate was found between femur and mandible in the early pregnancy group. 3. The absorption rate of fetus from late pregnancy group was significantly (P<0.01) higher than that of fetus from the early pregnancy group. 4. The high absorption rate was observed with femur and mandible in both groups of non-pregnanted and early pregnancy. 5. The significantly (P<0.01) high absorption rate was observed with ovary and fetus from the late pregnancy group.

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Study on The Distribution of Applied 32P into Different Forms of P Compounds in the Soils During Incubation (논·밭 토양(土壤)에 시용(柴用)된 32P의 토양중(土壤中) 상이(相異)한 인산화합물(燐酸化合物)로의 분배(分配)에 관(關)한 연구(硏究))

  • Hong, Jung-Kook;Hong, Chong-Woon;Park, Sang-Ji;Steenberg, Kjell
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.3
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    • pp.117-124
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    • 1979
  • The present work was carried out to study the fate of applied phosphorus labelled with $^{32}P$ and its availability to plants in soils subjected to different management practices. The results can be summarized as follows (Table 3): 1. The applied phosphorus was transformed into different phosphorus compounds in the soils depending upon the management practices and soil characteristics. 2. In the flooded paddy soil (pH 5.8) added P after one week of incubation was transformed into various fractions, the order of abundance being: Al-P> Ca-P$${\sim_\sim}$$Fe-P> Org.-P. After two weeks the order changed to: Fe-P> Al-P> Ca-P> Org.-P. The amounts of the Fe-P and Al-P fractions were found to increase from the second week of incubation whereas a decrease in Ca-P was noticed with the organic-P remaining constant. The amount of available P decreased from the first to the third week of incubation, but increased thereafter. 3. In the volcanic ash soil a major proportion of the applied phosphorus was found in the Fe-P fraction during the whole experimental period. The interconversions of the $^{32}P$ among the different phosphate fractions was not as evident as in the case of flooded rice soil. The recovery of applied P was low and remained constant throughout the incubation period. 4. In the upland soils relatively more of the applied phosphorus was found in the Ca-P fraction as compared with those of the other soils. As in the flooded paddy soil $^{32}P$ in the Ca-P fraction decreased with increasing incubation time, whereas in the Fe-P fraction it increased with time. The recovery of added phosphate as available P followed different patterns for the cultivated and the uncultivated soils. In the cultivated soils lit was relatively high and remained nearly constant during the whole incubation period. In the uncultivated soil on the other hand, it was high at the earlier time of incubation, but decreased with incubation time.

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Detection of DNA Adduct Formed by Mitomycin C by $^{32}P$-Postlabelling ($^{32}P$-Postlabelling 방법을 이용한 미토마이신 C에 의하여 형성된 DNA adduct의 검출)

  • Jeong, Hye-Yun;Kim, Jae-Hyeon;Park, Chang-Won;Lee, Dong-Gwon
    • YAKHAK HOEJI
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    • v.40 no.4
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    • pp.442-448
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    • 1996
  • Mitomycin C(MMC) has been used as an anticancer drug and behaves as an alkylating agent forming covalent cross-link between complementary strands of double strand DNA. The purpose of this research was to determine number of DNA adducts, formed in vivo by Mitomycin C, in mouse organs. DNAs from liver, lung, brain and pancreas were isolated and used for $^{32}P$-postlabelling. The labeled nucleotides were separated by 2D-TLC and subjected to autoradiography. Numbers of MMC-DNA adducts were 9,9,5,4 in liver, pancreas, lung and brain, respectively.

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$P^{32}$ Adsorption on Na-zeolite in Different Ionic Strengths (토양개량제(土壤改良劑)인 Zeolite에 의(依)한 인(燐)의 흡착(吸着))

  • Choi, Jyung
    • Applied Biological Chemistry
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    • v.25 no.2
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    • pp.99-104
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    • 1982
  • Natural zeolite rock was pulverized and dispersed in water. Clay fraction was collected by sedimentation method. The dominant clay mineral was Clinoptiolite with some Mordenite and Smectite. $P^{32}$ adsorption on Na-zeolite was determined in different ionic strengths using $P^{32}$ isotope by sludge method. The lower the pH of suspension, the longer the contact time, and the more the amount of zeolite, the more inorganic P was adsorbed by Na-zeolite, whereas the more P adsorption per unit gram of zeolite was observed at a 100mg addition than a 200mg in same volume of P-NaCl solution (20ml), indicating that the whole positively charged surface of Na-zeolite was not occupied by inorganic P. Furthermore, the more P adsorption on Na-zeolite was observed in higher ionic strength than in the lower. The maximum P adsorption on Na-zeolite was about 1me/g, and the zero point charge (ZPC) is assumed to be below pH 3.7.

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Effect of Dietary Sodium Nitrate Consumption on Egg Production, Egg Quality Characteristics and Some Blood Indices in Native Hens of West Azarbaijan Province

  • Safary, H.;Daneshyar, Mohsen
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.11
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    • pp.1611-1616
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    • 2012
  • The aim of the study was to evaluate the effect of sodium nitrate consumption on egg quality and quantity, and some blood parameters of native breeder hens of West Azerbaijan province. One hundred native hens were used from wk 25 to 32 of age. These birds were divided into two groups. One group was fed the control diet (CD) but the other fed the same diet supplemented with 4.2 g/kg sodium nitrate (ND). After 2 wks of adaptation, eggs were collected daily and egg mass and egg production were measured weekly for five weeks. To assess the egg quality parameters, two eggs from each replicate pen were collected for three consecutive days each week. At the end of experimental period (wk 32 of age), blood samples of 5 birds per replicate were collected from the wing vein into anticoagulant tubes. Dietary sodium nitrate didn't affect the egg production, shell stiffness, shell thickness and Haugh unit (p>0.05) but it decreased the both egg production and egg mass during the last three weeks (wks 30, 31 and 32) (p<0.05). Furthermore, a treatment effect was observed for yolk colour (p<0.05). Both the egg production and egg mass were increased over time (p<0.05). No significant treatment${\times}$time interaction was observed for egg weight, egg production and egg mass (p>0.05). No effect of time or treatment${\times}$time were observed for shell stiffness (p>0.05). Over time, shell thickness was decreased while Haugh unit increased (p<0.05). None of the blood TP and TG or the activity of ALT, AST and LDH enzymes were affected by dietary consumption of sodium nitrate at wk 32 of age (p>0.05). Sodium nitrite decreased both the TAC and TC at wk 32 of age (p<0.001). It was concluded that the lower body antioxidant capacity of nitrate fed birds resulted in the lower performance (egg weight, egg production and egg mass).

Interaction of the Bacteriophage P2 Tin Protein and Bacteriophage T4 gp32 Protein Inhibites Growth of Bacteriophage T4

  • Jin, Hee-Kyung;Kim, Min-Jung;Park, Chan-Hee;Park, Jung-Chan;Myung, Hee-Joon
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.724-726
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    • 2001
  • The growth of baceriophage T4 is inhibited by the presence of the tin gene product o bacteriophage P2. The interaction between purified Tin and gp32 proteins was observed using coimmunoprecipitation experiments. The in vivo interaction was confirmed by yeast two-hybrid experiments. A deletion analysis showed that the Asp 163 region of gp32 to DNA substrates was not affected by the presence of Tin, Thus, it would appear that the inhibition of 4 growth by Tin was due to a protein-protein interaction rather than affecting the DNA-binding ability of gp32.

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Sucrose-permeability Induced by Reconstituted Connexin32 in Liposomes.

  • Rhee, Senng-Keun;Hong, Eun-Jnng
    • BMB Reports
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    • v.28 no.2
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    • pp.184-190
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    • 1995
  • Functional study of the gap junction channel has been hindered by its inaccessibility in situ. Identification of forms of this channel in artificial membrane has been elusive because of the lack of identifying channel physiology. Connexin32 forms gap junction channels between neighboring cells in rat liver. Connexin32 was affinity-purified using a monoclonal antibody and reconstituted into artificial phospholipid vesicles. The reconstituted connexin32 formed channels through the vesicle membrane that were permeable to sucrose (Stokes radius: $5{\AA}$). The permeability to sucrose was reversibly reduced by acidic pH. In addition, the pH effect on the permeability to sucrose fit well with by the Hill's equation (where, n=2.7 and pK=6.7).

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Effect of Molecular Weight of Chitosan on Flocculation of Suspended Solids in Rhodamine 6G-Sodium Dodecyl Sulfate System (키토산 분자량에 따른 Rhodamine 6G-Sodium Dodecyl Sulfate계의 응집효과에 관한 연구)

  • Kim, Sung Hyun;Nah, Jae Woon;Oh, Jeong Sun;Song, Ki Dong
    • Journal of the Korean Chemical Society
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    • v.42 no.6
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    • pp.672-678
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    • 1998
  • Chitin was extracted from crab shell of Portuns triberculatus and deacethylated to yield chitosan with various molecular weights. The absorption and the fluorescence spectra of Rhodamine 6G(Rh 6G)-sodium dodecyl sulfate(SDS) and Rh 6G-chitosan systems were obtained. From the spectra, we observed that the absorption and the fluorescence intensity of Rh 6G-SDS system decreased when S/D(the concentration of SDS to that of Rh 6G ratio) was below or at 32, while they increased when S/D was above 32. From the suspended solid(SS) removal rate and the transmittance of Rh 6G-SDS-chitosan system, we found that when S/D ratio was 32 its flocculating behaviour was much stronger than Rh 6G-SDS system. As the concentration and the molecular weight of chitosan increased, we also found that S/D range was extended from 32 to 100. With increasing the molecular weight of chitosan, the SS removal rate increased around pH 2~9 but decreased remarkably at pH>12.

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Development and Testing of Day Care Program for Demented Elders (주간보호센터 중심의 재가 치매노인을 위한 프로그램의 개발 및 효과 검증 연구)

  • Lim, Young-Mi;Jeong, Bok-Hee
    • Journal of Korean Public Health Nursing
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    • v.19 no.2
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    • pp.371-381
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    • 2005
  • Purpose: The purpose of the study was to develop the day care program for the demented elders and to determine the effects of multiple activity therapies on the physical, emotional, cognitive, social function and physiological indicators of dementia elders in day care center. Method: A total of 13 elders with mild dementia in the data care center were participated. Manual and guideline of data care program was developed. Data care program was composed of movement/exercise, foot massage, music therapy, horticultural therapy, and art therapy. Data care program was performed by trained intervener for 32 weeks. Instruments were physical functioning such as hand grip strength., vital capacity, chiar stand test, sit and reach test, one leg standing, Berg' Balance scale, 6m Walk Velocity, stair climb, ADL, GDS-K, MMSE-K, Integrative Social Functioning Scale, cortisol, Ig A and Ig G. These were measured at the baseline, 16-week, and 32-week post-intervention by the trained nurses. Data were analyzed as Wilcoxon signed rank test. Results: For Hand grip, balance, and vital capacity, there was a significant difference between baseline and 16-week post-intervention(p<.05). For one leg standing, 6m velocity, there was a significant difference between 16-week post-intervention and 32-week post-intervention(p<.05). For Stair climb, there was a significant difference between baseline and 32-week post-intervention(p<.05). For depression and social functioning, there was no significant difference among baseline, 16-week, and 32-week post-intervention. For cognitive functioning, there was a significant difference between baseline and 32-week post intervention(p<.05). There was a significant difference of cortisol, and Ig G between baseline and 32-week post-intervention(p<.05). Conclusion: It would appear that physical fitness, functional performance, ADL, depression, social and cognitive functioning, and physiological indicator of community dwelling elders with dementia can be improved through day care program using multiple stimuli.

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