Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Interaction of the Bacteriophage P2 Tin Protein and Bacteriophage T4 gp32 Protein Inhibites Growth of Bacteriophage T4

  • Jin, Hee-Kyung (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Min-Jung (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Park, Chan-Hee (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Park, Jung-Chan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Myung, Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • Published : 2001.08.01
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Abstract

The growth of baceriophage T4 is inhibited by the presence of the tin gene product o bacteriophage P2. The interaction between purified Tin and gp32 proteins was observed using coimmunoprecipitation experiments. The in vivo interaction was confirmed by yeast two-hybrid experiments. A deletion analysis showed that the Asp 163 region of gp32 to DNA substrates was not affected by the presence of Tin, Thus, it would appear that the inhibition of 4 growth by Tin was due to a protein-protein interaction rather than affecting the DNA-binding ability of gp32.

Keywords

References

  1. DNA repair In:Bacteriophage T4 Bernstein, C.;S. S. Wallac
  2. Cold Spring Harbor Symp. Quant. Biol. v.18 Lysogenic versus lytic cycle of phage multiplication Bertani, G.
  3. J. Mol. Biol. v.112 Multiple interaction of a DNA binding protein in vivo. Ⅰ. Gene 32 mutations in phage T4 inactivate different steps in DNA replication and recombination Breschklin, A.;G. Mosig
  4. J. Mol. Biol. v.112 Multiple interactions of a DNA binding protein in vivo. Ⅱ. Effects of host mutations in DNA replication of phage T4 gene 32 mutations Breschklin, A.;G. Mosig
  5. Molecular Biology of Bacteriophage T4 Restriction and modification Carlson, K.;E.A. Raleigh;S. Hattman;J. Karama(et al.)(eds.)
  6. The Biology of Nonspecific DNA-Protein Interactions T4 bacteriophage gene 32 protein Karpel, R. L.;A. Revzin(ed.)
  7. J. Microbiol. Biotechnol. v.10 Implications of exonuclease activity of bacteriphage P2 Old protein for lambda exclusion Kim, K.;C. Park;H. Yeo;Y. Kee;J. Park;H. Myung
  8. Molecular Biology of Bacteriophage T4 Inhibition of DNA replication Kreuzer, K.N.;S.W. Morrical;J. Karam(et al.)(eds.)
  9. Genetics v.108 identification and characterization of the alc gene product of bacteriophage Kutter, E.;R. Divdahl;K. Rand
  10. Virology v.3 Suppression of multiplication of heterologous bacteriophages in lysogenic bacteria lederberg, S.
  11. Encyclopedia of Virology v.3 T4 bacteriophage and related bacteriophages Mosig, G.;R.G. Webster(et al.)(eds.)
  12. Virology v.230 A novel mechanism of virus-virus interactions:Bacteriophage P2 Tin protein inhibits phage T4 DNA synthesis by poisoning the T4 single-stranded DNA binding protein gp32 Mosig, G.;S. Yu;H. Myung;E. Haggard-Ljunquist;L. Davenport;K. Carlson;R. Calendar
  13. Nature v.376 Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) cmplexed to DNA Shamoo, Y.;A.M. Freidman;M.R. Parson;W.H. Konigsberg;T. A. Steitz
  14. Nature v.376 Crystal structure of a replication fork single-stranded DNA binding protein(T4 gp32) complexed to DNA (Erratum) Shamoo, Y.;A.M. Freidman;M.R. Parson;W.H. Konigsberg;T. A. Steitz
  15. Mol. Microbiol. v.15 Phage-exclusion enzymes: A bonanza of biochemical and cell biology reagents? Snyder, L.
  16. Proc. Natl. Acad. Sci. USA v.91 Translational elongation factor Tu cleaved by a phage exclusion system Yu, Y.-T.;N. Snyder