• Proceedings of the PSK Conference
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• 2002.10a
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• pp.232.1-232.1
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• 2002

Methods of 14C-radio isotope labeling of quinolone intermediates at four different sites are described. 14C-radio isotope labeled quinolone intermediates can be synthesized from 14C-1-malonic acid, 14C-2-malonic acid, 14C-benzene ring. and 14C-trimethyl orthoformate. The major site of 14C-radio isotope labeled quinolone intermediates is from 14C-2-malonic acid. We want to help customers to choose the best way for synthesis of 14C-radio isotope labeled quinolone derivatives. and give a general comprehension for 14C-radio isotope labeled pharmaceutical compounds. (omitted)

• Journal of Radiation Industry
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• v.17 no.2
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• pp.127-134
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• 2023

Pharmacokinetic (PK) data provide pivotal information in drug development, and they are usually first studied in the preclinical stage using various animals. However, quite often, animal PK data may not match with human PK, especially in metabolites. Thus, most regulatory agencies in the world make it mandatory to obtain metabolite information using ¹⁴C radiolabeled drug in human for small molecule drug candidates. However, such studies are expensive and time consuming and they are usually done at the end of Phase II trials using ~3.7 MBq of ¹⁴C labeled drug in a limited number of human subjects. Introduction of accelerator mass spectrometry (AMS) in this kind of study has revolutionized it. Since AMS can measure ¹⁴C level as close as natural abundance, it can quantify the amounts of ¹⁴C labeled drugs and their metabolites produced in human body that consumes less than the amount of 0.0037 MBq of ¹⁴C labeled drug, a very safe level of radioactive dose in human. Therefore, it is now possible to conduct human ¹⁴C studies safely in early clinical trials without spending hefty amount of money and time. Korea Radioisotope Center for Pharmaceuticals(KRICP) at Korea Institute of Biological and Medical Sciences(KIRAMS) has established an AMS facility in 2018, housing a 0.5MV AMS manufactured at the US National Electrostatics Corps (NEC). The AMS instrument has been validated using various standard samples that have been prepared at Lawrence Livermore National Laboratory in the US, a worldly reputable provider of AMS standards. In this paper, we present a mass balance study for acetaminophen in rats using AMS and prove that the study results are equivalent with those of literature, which shows the AMS facilities at KRICP has successfully installed and be ready to be used in the various PK studies using ¹⁴C labelled compounds for new drug development.

• Animal cells and systems
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• v.14 no.4
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• pp.297-303
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• 2010

To document the basic ecological aspects of Chaoborus species, which has never been reported in Korea, we attempted to identify the species, to monitor seasonal and vertical dynamics, and to elucidate trophic relations of the species in Sang-Chun reservoir. Using morphological characteristics, we identified the collected samples as C. flavicans. Also, we compared the distribution of C. flavicans, Daphnia rosea and chlorophyll a to observed seasonal dynamics. The increase of C. flavicans was observed 1-2 weeks after the increase of D. rosea. Survey of diel vertica migration patterns in the summer season showed that C. flavicans were in hypolimnion at daytime, but moved to the epilimnion at night. Finally, to determine trophic relationships in Sang-Chun reservoir, additional studies on the food web were undertaken by stable isotope analysis. Chaoborus flavicans I-II instars appear to be filter feeders based on carbon isotope values. Trophic levels of C. flavicans III-IV instars were shown to be higher than other zooplankto based on nitrogen isotope values.

• Bulletin of the Korean Chemical Society
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• v.32 no.10
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• pp.3618-3623
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• 2011

This paper presents a stable isotope chemistry of bone collagen and carbonate. Bone carbonate has the potential to provide additional isotopic information. However, it remains controversial as to whether archaeological bone carbonate retains its original biogenic signature. I used a novel application of bone density fractionation and checked the integrity of ${\delta}^{13}C_{apa}$ values using radiocarbon dating. Diagenesis in archaeological bone carbonate still remains to be resolved in extracting biogenic information. The combined use of bone density fractionation and differential dissolution method shows a large shift in the ${\delta}^{13}C_{apa}$ values. Although ${\delta}^{13}C_{apa}$ values are improved in lighter density fractions, a large percentage of contamination in bone carbonate was reported via $^{14}C$ dating compared to that noted with bone collagen.

• Journal of the Korean Chemical Society
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• v.14 no.1
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• pp.49-50
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• 1970

A wet oxidation method of oxalic acid standard used in radiocarbon dating is described, and error due to possible carbon isotope fractionation during the oxidation is estimated.

• Analytical Science and Technology
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• v.25 no.5
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• pp.300-306
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• 2012

This study reports the stable carbon and nitrogen isotope of mummy, the preserved human time capsule from Joseon Dynasty. The stable isotopic composition of biological tissue is expected to be a direct and constant function of diet because the stable isotope ratios do not decay or change in abundance through time. Over the past three decades, stable carbon and nitrogen isotope analysis has been extensively applied for archaeological topics. However, this is the first case in Korean mummy to extract palaeodietary information using stable isotope analysis. We extracted isotopic information from bone collagen and hair keratin, which reflects the lifetime average diet and short-term diet, respectively. The average values of ${\delta}^{13}C$ and ${\delta}^{15}N$ values are -19.0‰ and 11.4‰ (bone collagen), and -21.3‰ and 14.3‰ (hair keratin). Stable carbon isotope shows a mainly $C_3$-based diet from cradle to grave. On the other hand, there is significant difference in stable nitrogen isotope which implies a relatively enriched diet before death. We expect this study opens up a potential to understand the palaeodietary lifestyle in Joseon Dynasty.

• Journal of the Korean Physical Society
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• v.73 no.9
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• pp.1247-1254
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• 2018

We study the uncertainty stemming from different theoretical models in the spectroscopic factors extracted from experiments. We use three theoretical approaches, the distorted wave Born approximation (DWBA), the adiabatic distorted wave approximation (ADWA) and the continuum discretized coupled-channels method (CDCC), and analyze the $^{12}C(d,p)^{13}C$, $^{14}C(d,p)^{15}C$ reactions. We find that the uncertainty associated with the adopted theoretical models is less than 20%. We also investigate the contribution from the remnant term and observe that it gives less than 10% uncertainty. We finally make an attempt to explain the discrepancy in the spectroscopic factors of $^{17}C(\frac{3}{2}^+)$ between the ones extracted from experiments and from shell model calculations by analyzing the $^{16}C(d,p)^{17}C$ reaction.

• Molecular & Cellular Toxicology
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• v.2 no.1
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• Journal of the Korean Magnetic Resonance Society
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• v.14 no.2
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• pp.117-126
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• 2010

Hsp33 is a molecular chaperone achieving a holdase activity upon response to a dual stress by heat and oxidation. Despite several crystal structures available, the activation process is not clearly understood, because the structure inactive Hsp33 as its reduced, zinc-bound, monomeric form has not been solved yet. Thus, we initiated structural investigation of the reduced Hsp33 monomer by NMR. In this study, to overcome the high molecular weight (33 kDa), the protein was triply isotope-[$^{13}C$, $^{15}N$, $^2H$]-labeled and its inactive, monomeric state was ensured. 2D-[$^1H$, $^{15}N$]-TROSY and a series of triple resonance spectra could be successfully obtained on a high-field (900 MHz) NMR machine with a cryoprobe. However, under all of the different conditions tested, the number of resonances observed was significantly less than that expected from the amino acid sequence. Thus, a possible contribution of dynamic conformational exchange leading to a line broadening is suggested that might be important for activation process of Hsp33.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.4
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• pp.259-273
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• 2021

We conducted a study to investigate the characteristics of the carbon cycle of two streams (located in Shig a Prefecture, Japan), having similar size, namely, the Adokawa stream (length: 52 km, area: 305 km², watershed population: 8,000) and the Yasukawa stream (length: 62 km, area: 380 km², watershed population: 120,000), but with different degree of human activity. Samples were collected from these two streams at 14 (Adokawa stream) and 23 (Yasukawa stream) stations in the flowing direction. The dissolved inorganic carbon (DIC) concentration and the stable carbon isotope ratio of DIC (δ¹³C-DIC) were measured in addition to the watershed features and the chemical variables of the stream water. The δ¹³C-DIC (-9.50 ± 2.54‰), DIC concentration (249 ± 76 µM), and electric conductivity (52 ± 13 µS/cm) in Adokawa stream showed small variations from upstream to downstream. However, the δ¹³C-DIC (-8.68 ± 2.3‰) upstream of Yasukawa stream was similar to that of Adokawa stream and decreased downstream (-12.13 ± 0.43‰). DIC concentration (upstream: 272 ± 89 µM, downstream: 690 ± 37 µM) and electric conductivity (upstream: 69 ± 17 µS/cm, downstream: 193 ± 37 µS/cm) were higher downstream than upstream of Yasukawa stream. The DIC concentration of Yasukawa stream was significantly correlated with watershed environmental variables, such as, watershed population density (r = 0.8581, p<0.0001, n = 23), and forest area percentage of the watershed (r = -0.9188, p<0.0001, n = 23). δ¹³C-DIC showed significant negative correlation with the DIC concentration (r = -0.7734, p<0.0001, n = 23), electric conductivity (r = -0.5396, p = 0.0079, n = 23), and watershed population density (r = -0.6836, p = 0.0003, n = 23). Our approach using a stable carbon isotope ratio suggests that DIC concentration and δ¹³C-DIC could be used as indicators for monitoring the health of stream ecosystems with different watershed characteristics.