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Risk Assessment Aluminum Levels of Circulating Agricultural Products in Korea (국내 다소비 농산물의 알루미늄 농도 모니터링 및 농산물 섭취로 인한 위해도 평가)

  • An, Jae-Min;Hong, Kyong-Suk;Kim, Sung-Youn;Kim, Ji-Hye;Yu, Kyong-Eun;Kim, Hyo-Young;Park, Hyoung-Dal;Lee, Jae-Hwon;Kim, Dong-Ho
    • Korean Journal of Environmental Agriculture
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    • v.34 no.4
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    • pp.336-344
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    • 2015
  • BACKGROUND: This study was carried out to collect occurrence data on aluminum in 12 type agricultural products and assess dietary exposure risk to the Korean population health for aluminum concentration in agricultural products.METHODS AND RESULTS: Aluminum analysis samples were performed using microwave device and Inductively Coupled Plasma Optical Emission Spectrometer. The LOD(Limit of Detection) for aluminum was 0.851 μg/kg, while the LOQ(Limit of Quantitation) was 2.838 μg/kg and recovery was 97.6% for aluminum. The average levels of aluminum in mg/kg were 0.526 for rice, 0.546 for Korean cabbage, 1.316 for corn, 6.207 for soybean, 0.549 for sweet potato, 0.257 for potato, 6.963 for spinach, 1.213 for carrot, 0.524 for garlic, 0.950 for radish, 1.015 for leek, and 3.511 for Welsh onion. The dietary exposures of aluminum through usual intake were polished rice 89.31 μg/day, Korean cabbage 33.14 μg/day, corn 0.66 μg/day, soybean 3.72 μg/day, sweet potato 6.86 μg/day, potato 4.96 μg/day, spinach 45.96 μg/day, carrot 6.79 μg/day, garlic 2.36 μ g/day, radish 7.32 μg/day, leek 2.23 μg/day and Welsh onion 43.89 μg/day, taking 0.57%, 0.21%, 0.00%, 0.02%, 0.04%, 0.03%, 0.04%, 0.04%, 0.02%, 0.05%, 0.01% and 0.28% of PTWI(2 mg/kg b.w./week), respectively.CONCLUSION: The levels of overall dietary exposure to aluminum for Korean population through intake of agricultural product was far below the recommended JECFA level, indicating of least possibility of risk.

Seasonal Variations of Water Environments and Benthic Diatom Communities in Streams across Byeonsan-Bando and Seonunsan Parklands in Jeollabukdo, Korea (전라북도의 변산반도 국립공원과 선운산 도립공원 하천의 수환경과 부착규조류 군집의 계절적인 변화)

  • Park, Kyung-Woo;Kim, Yun-Sam;Park, Jung-Won;Jeune, Kyung-Hee;Kim, Mi-Kyung
    • Korean Journal of Ecology and Environment
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    • v.44 no.2
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    • pp.239-251
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    • 2011
  • To compare the different water environments in the Jigsaw stream (st. 1, 2) and Jujin stream (st. 3, 4) crossing over the Byeonsan-Bando and Seonunsan parklands, the physico-chemical factors and the species compositions of epilithic diatoms were analyzed from March 2009 to February 2010. The mean values of conductivity (681.1 ${\mu}S\;cm^{-1}$), suspended solids (27.83 mg $L^{-1}$), biological oxygen demand (3.15 mg $L^{-1}$), total nitrogen (3.24 mg $L^{-1}$), total phosphorus (0.24 mg $L^{-1}$) and chlorophyll-${\alpha}$ (12.99 mg $m^{-3}$ ) in Jujin stream were significantly higher than each element in Jigsaw stream. Eighty taxa were classified into 73 species and seven varieties belonging to 24 genera, eight families, three suborders and two orders in Jigsaw stream. Eighty eight taxa were classified into 81 species and seven varieties belonging to 22 genera, seven families, three suborders and two orders in Jujin stream. Biological water quality assessments revealed that Jigsaw stream was ${\beta}$~${\alpha}$-oligosaprobic and Jujin stream was ${\beta}$-mesosaprobic. Dominant and diversity indices were indicated from 0.25~0.81 and from 1.73~4.14 in Jigsaw stream, respectively, and from 0.29~0.64 and from 2.72~4.02 in Jujin stream, respectively. Jujin stream was more eutrophic than those of Jigsaw stream. The different water environments between the two streams could be due to the different ecosystems of the neighboring basins of each stream crossing over in the mountain. Further studies should investigate the water environments of two streams by continuous and regular monitoring to analyze the different ecosystem mechanisms.

Studies on the Dietary Fiber of Brown Rice and Milled Rice (현미와 백미의 식이섬유에 관한 연구)

  • Lee, Hee-Ja;Byun, Si-Myung;Kim, Hyong-Soo
    • Korean Journal of Food Science and Technology
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    • v.20 no.4
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    • pp.576-584
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    • 1988
  • Neutral detergent fiber(NDF) were extracted from the Nampung, Milyang #23, Whasung and Jinhung varieties of rice by neutral detergent fiber method. To determine the properties of NDF three factors were measured : water-binding capacity(WBC), Fe-binding capacity and sodium taurocholate binding with NDF. The average WBC of NDF was $5.60{\pm}0.87gH_2O/g$ NDF, and the average Fe-binding capacity ranged from 24.63% at pH 5.0 to 19.6% at pH 6.0 and 48.98% at pH 7.0. Binding of sodium taurocholate with NDF was determined in vitro using C-14 labeled sodium taurocholate at 100M sodium taurocholate concentrations. NDF binding of Jinhung was 27.87 while Nampung, Milyang #23 and Whasung measured 32% each. When sodium taurocholate concentrations were raised from $40{\mu}M\;to\;240{\mu}M$ the tendency of binding increased as the concentrations increased, but not linearly.

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Antioxidative, Antimicrobial and Anticytotoxic Activities of Seungmagalgeuntang and Fermented Seungmagalgeuntang (승마갈근탕과 발효 승마갈근탕에 의한 항산화, 항미생물 및 항세포독성 효과)

  • In, Jae Pyung;Shin, Jung Mi;Hur, Sun Jin;Lee, Si Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.7
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    • pp.980-988
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    • 2014
  • Seungmagalgeuntang (SG) is broadly used in traditional Oriental medicine especially in Korea, China, and Japan, for its many pharmacological effects. This study was carried out to investigate the antioxidative, antimicrobial, and anticytotoxic activities of SG and fermented seungmagalgeuntang (FSG). DPPH radical scavenging activities of SG and FSG were 70% and 74%, respectively, which increased slightly by fermentation. Nitrite scavenging activities were strongly altered at pH 1.2, (36.4% in SG and 38.3% in FSG) by addition of $200{\mu}g/g$. Superoxide dismutase-like activities were from 21.5% to 23.3% at a concentration of 0.4 mg/mL, and the highest value were observed in FSG. Total flavonoid contents of SG and FSG were 47.1 and $52.1{\mu}g/L$, respectively which shows an increase upon fermentation. In the antimicrobial activity test, $MIC_{50}$ values of SG and FSG were $800{\mu}g/mL$ for Candida albicans and 3,200 and $1,600{\mu}g/mL$ for Staphylococcus epidermidis and Staphylococcus aureus, respectively. Antibacterial effects were higher in FSG compared to SG. Anticytotoxic cadmium toxicities ranged from 63.5% to 76.1% at $10{\mu}g/mL$ of SG and FSG, and the highest value was observed in FSG. In the sensory evaluation, color, flavor, and overall preference values were higher in FSG.

An experimental study of the strength and internal structure of solder joint of fixed partial denture (가공의치(架工義齒) 납착부(蠟着部)의 강도(强度)와 내부구조(內部構造)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Park, Sang-Nam;Kay, Kee-Sung
    • The Journal of Korean Academy of Prosthodontics
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    • v.23 no.1
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    • pp.39-59
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    • 1985
  • The purpose of this study was to investigate how gap distances of 0.13mm, 0.15mm, 0.20mm, and 0.30mm affects solder joint strength from gold alloys and nickel-chromium base alloys and to examine the composition of solder gold, the solder joint of gold alloys and nickel-chromium base alloys. The tensile test specimens were prepared in the split stainless steel mold with a half dumbbell shape 2.5mm in diameter and l2mm in length. 6 pairs of specimens of each gap distance group of gold alloys and nickel-chromium base alloys were made and 48 pairs of all specimens were soldered with solder gold of 666 fineness. All soldered specimens were machined to a uniform diameter and then a tensile load was applied at a cross-head speed of 0.10mm/min using Instron Universal Testing Machine, Model 1115. The fractured specimens at solder gold of solder joint fracture with each gap distance of 0.13mm, 0.15mm, 0.20mm, and 0.30mm were examined under the Scanning Electron Microscope, JSM-35c and the composition of solder gold, the solder joint of gold alloys and nickel-chromium base alloys was analyzed by Electron Probe Micro Analyzer. The results of this study were obtained as follows: 1. In case of soldering of gold alloys, the tensile strength between gold alloys showed $37.33{\pm}2.52kg/mm^2$ at 0.13, $39.14{\pm}3.35kg/mm^2$ at 0.15mm, $43.76{\pm}2.97kg/mm^2$ at 0.20mm, and $49.18{\pm}4.60kg/mm^2$ at 0.30mm. There was statistically significant difference at each gap distance, and so the greater increase of gap distance showed the greater tensile strength. 2. In case of soldering of nickel-chromium base alloys, the tensile strength between nickel-chromium base alloys showed $34.84{\pm}4.26kg/mm^2$ at 0.13mm, $37.25{\pm}2.49kg/mm^2$ at 0.15mm, $42.91{\pm}4.32kg/mm^2$ at 0.20mm, and $46.93{\pm}4.21kg/mm^2$ at 0.30mm. There was not statistically significant difference only between 0.13mm and 0.15mm and bet ween 0.20 mm and 0.30mm, but generally the greater increase of gap distance showed the greater tensile strength. 3. The greater increase of gap distance shoed less porosities in solder gold at solder joint fracture. 4. In solder gold Au, Cu, Ag, Zn, and Sn were composed and Au and Cu were mostly distributed uniformly. 5. In solder joints of solder gold and gold alloys Au, Cu, Ag, Zn, and Sn were composed in solder gold and Au, Cu, Ag, Pt, and Pd were composed in gold alloys. Au and Cu of solder gold and gold alloys were mostly distributed uniformly and the diffusion of other elements except Pt and Pd around the solder joint was not almost found. In solder joints of solder gold and nickel-chromium base alloys Au, Cu, Ag, Zn, and Sn were composed in solder gold and Ni, Cr, and Al were composed in nickel-chromium base alloys. Au and Cu of solder gold and Ni and Cr of nickel-chromium base alloys were mostly distributed uniformly and the diffusion of other elements except Cr around the solder joint was not almost found.

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Comparison in Serum Constituents of Cultured Marine Fishes in Early Summer Season (주요 양식어류의 하절기 혈액성분 비교)

  • 전중균;김형배
    • Journal of Aquaculture
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    • v.11 no.4
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    • pp.547-556
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    • 1998
  • This study was performed to obtain the basic data on the serum constituents of several marine fish spesies commonly cultured in Korea. Blood samples taken from six species of fish were analyzed for various components of serum, total protein (TP), albumin (ALB), triglyceride (TRIG), cholesterol (CHOL), glucose (GLC), lipase (LIPA), amylase (AMYL), aspartate transaminoferase (AST), sodium (Na), potassium (K), chloride (CI) and phosphorus (PHOS). The fish used were coho salmon (Oncorhynchus kisutch), rock fish (Sebastes schlegeli), sea bass (Lateolabrax japonicus), olive flounder (Paralichthys olivaceus), rock fish (Sebastes schlegeli), sea bass (Lateolabrax japonicus), olive flounder (Paralichthys olivaceus), parrot fish (Oplegnathus fasciatus) and jack mackerel (Trachurus jaonicus) reared at the Chungmu Experimental Fish Culture Station of KORDI when the water tempetature was ca. 16.5$^{\circ}C$. There were significant differences in TRIG, CHOL, LIPA and AMYZ among the species analyzed. TRIG concentratin were ranged 178~180mg/dl in jack mackeerel and rock fish, 126~159 mg/dl in olive flounder and sea bass, and 102~114 mg/dl in coho salmon and parrot fish, respectively. Jack mackerel showed the highest levels in CHOL (255mg/dl) and GLC(138mg/dl) among species. LIPA levels were recorded 256 U/dl in coho salmon, 41~42 U/dl in parrot fish and rock fisk, and 5~11 U/dl jack mackerel and sea bass, respectively. AMYL activity of coho salmon was measured as 2, 665 U/dl, and that of jack mackerel was 1,210 U/dl while sea bass showed 60 U/dl and parrot fish, olive flounder and rock fish had at most 5 U/dl. On the other hand, there was no significant difference in the concentration of Na and CI. Na and K were proved that they were negatively correlated in all the species. Generally, among blood components, PHOS and CHOL levels were different depending on environmental temperature of each fish species, especially in olive flounder. Rock fish and parrot fish showed high blood concentration of those components during low temperature period while olive flounder and jack mackerel reached high level during their optimal environmental temperature period. The electrolyte concentration and LIPA activity were high during low water temperature period, in general, but TP and ALB concentrations were high during optimal temperature period. The concentrations of TRIG, CHOL and GLC, those which were used as energy sourses, were different among species by season.

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Preparation of Fermented Milk Added with Powder of Opuntia ficus-indica var. saboten and Its Sensory Characteristics (백년초 분말을 첨가한 발효유 제조 및 관능적 특성)

  • Lee, Jo-Yoon;Bae, Hyoung-Churl
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.6
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    • pp.967-974
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    • 2009
  • The effects of Opuntia ficus-indica var. saboten powder on the growth of lactic acid bacteria (LAB) were investigated in order to explore the possibility of manufacturing fermented milk containing the powder. Differences in pH, acidity, LAB counts, viscosity, and sensory evaluation were measured. Also the effects of dietary supplementation on the growth of piglets were evaluated by feeding fermented milks containing 0.1%, 0.2%, 0.5%, and 1.0% Opuntia ficus-indica var. saboten powder. Levels of pH, titratable acidity, viable LAB counts and viscosity were significantly different by the addition of the powder. When fermented milks containg 0.1%, 0.2%, 0.5% and 1.0% Opuntia ficus-indica var. saboten powder were kept at $4^{\circ}C$ for 30 days, viable LAB counts remained high after 30 days of storage. The effects of dietary supplementation of 0.2% Opuntia ficus-indica var. saboten fermented milk were investigated by examining piglet growth rate and fecal ammonia gas release. The piglets were fed 100g/herd of the fermented milk for 14 days. Average daily body weight gain was significantly (p<0.05) improved (110%) with dietary supplementation of the fermented milk, compared to a control group. More over, fecal ammonia gas emissions were reduced by dietary supplementation of the Opuntia ficus-indica var. saboten fermented milk. Sensory evaluation results showed that the samples containing 0.1% and 0.2% Opuntia ficus-indica var. saboten powder had the most parameters similar to those of the control.

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Chondrogenesis of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood (사람 제대혈 유래 간엽줄기세포로부터 연골세포 분화)

  • Koh, Phil-Ok;Cho, Jae-Hyun;Nho, Kyoung-Hwan;Cha, Yun-Im;Kim, Young-Ki;Cho, Eun-Hae;Lee, Hee-Chun;Jung, Tae-Sung;Yeon, Seong-Chan;Kang, Kyung-Sun;Lee, Hyo-Jong
    • Journal of Veterinary Clinics
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    • v.26 no.6
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    • pp.528-533
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    • 2009
  • In the current study, the mesenchymal stem cells (MSCs) isolated and propagated from the human umbilical cord blood (UCB) were tested for their capabilities of differentiation into chondrocytes in vitro. The mesenchymal progenitor cells (MPCs) collected from UCB were cultured in a low glucose DMEM medium with 10% FBS, L-glutamine and antibiotics. The human MSC colonies were positively stained by PAS reaction. When the immunophenotypes of surface antigens on the MSCs were analyzed by fluorescence-activated cell sorter (FACS) analysis, these cells expressed positively MSC-related antigens of CD 29, CD44, CD 90 and CD105, whereas they did not express antigens of CD14, CD31, CD34, CD45, CD133 and HLA-DR. Following induction these MSCs into chondrocytes in the chondrogenic differentiation medium for 3 weeks or more, the cells were stained positively with safranin O. We clearly confirmed that human MSCs were successfully differentiated into chondrocytes by RT-PCR and immunofluorescent stain of type-II collagen protein. These data also indicate that the isolation, proliferation and differentiation of the hUCB-derived MSCs in vitro can be used for elucidating the mechanisms involved in chondrogenesis. Moreover this differentiation technique can be applied to developing cell-based tissue regeneration or repair damaged tissues.

Comparison of quality changes in brined cabbage with deep sea water salt and a commercial brined cabbage product (해양심층수염 절임배추와 시판 절임배추의 품질변화 비교)

  • Lim, Ji Hoon;Jung, Jee Hee;Kim, Dong Soo;Kim, Young Myoung;Kim, Byoung Mok
    • Food Science and Preservation
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    • v.21 no.5
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    • pp.676-687
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    • 2014
  • This study investigated the quality changes in cabbage brined with deep sea water salt and in a commercial brined cabbage product. The subject cabbages were separated into two groups: those manufactured in the Lab (ML) and the commercial brined cabbage product (CP). Each group had three brining treatments: with sun-dried salt (S, CS), refined salt (R, CR), and deep sea water salt (D, CD). The salinity level of the ML group was 2.1~2.3%, higher than that of the CP group (1.1~1.5%). The total plate count (TPC) was detected as 5.0 log CFU/g with the S, R, and D treatments at Day 7, but the growth rate of the TPC with the CS, CR, and CD treatments was faster than that with the S, R, and D treatments (6.9~7.7 log CFU/g). A lactic acid bacteria (LAB) level of 5.0~6.6 log CFU/g was also detected in the S, R, and D samples, but only 7.0~7.6 log CFU/g was detected in the CP groups at Day 14. The instrumental hardness levels of the cabbage brined with the deep sea water salts (D and CD) were 3,971 g and 3,932.4 g, respectively, which were significantly higher than those of the samples that were salted with sun-dried salt and refined salt (p<0.05). As for the sensory attributes, S, D, and CD maintained their marketability scores until the end of the storage period for all the properties. CD presented the highest total free amino acid (478.9 mg%), glutamic acid (107.0 mg%), citric acid (428 mg%), and sodium (189 ppm) contents.

A Study of Mode of Action of Fluazifop-butyl- II. Fluazifop-butyl Effect on Cell Division, Cell Enlargement, and Protein Synthesis in Oat(Avena sativa L.) Roots (Fluazifop-butyl의 제초기구(除草機構)에 관한 연구(硏究)- 제2보(第II報) Fluazifop-butyl이 귀리뿌리의 세포분열(細胞分裂), 세포신장(細胞伸張) 및 단백질합성(蛋白質合成)에 미치는 영향(影響))

  • Kim, Jae-Cheol
    • Korean Journal of Weed Science
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    • v.6 no.2
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    • pp.168-173
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    • 1986
  • The effects of varying concentrations and duration of fluazifop-butyl [(${\pm}$)-butyl [2- [4- [(5-(trifluoro methyl)-2-pyridinyl] oxy] phenoxy] propanate] treatment on cell division, cell enlargement, and protein synthesis were studied. Oat (Avena staiva L.) were treated from 0 to 48 hr with concentration ranging from $1{\times}10^{-6}M$ to $1{\times}10^{-3}M$ of fluazifop-butyl in the cell division study. There was a significant reduction in the mitotic indices of oat roots treated with $1{\times}10^{-4}M$ after 6 hr. After 18 hr treatment, All herbicide treatment inhibited cell division significantly. After 24 hr treatment almost 100% inhibition of cell division occurred at $1{\times}10^{-4}M$ to $1{\times}10^{-3}M$ while 20% inhibition of cell division occurred at $1{\times}10^{-6}M$ concentration at same exposure period. The greatest inhibition of cell division occurred between 0 to 18 hr. The avena coleoptile straight- growth test were used to determine the influence of fluazifop-butyl on eoleoptile growth. Significant inhibition of elongation of oat coleoptiles were observed at $1{\times}10^{-7}M$ to $1{\times}10^{-3}M$ after 24 hr incubation. Protein incorporation study showed that the $1{\times}10^{-4}M$ of fluazifop-butyl caused 60% inhibition of protein synthesis. It was concluded that the growth of inhibition of plants caused by fluazifop-butyl results from inhibition of cell division, cell enlargement, and protein synthesis.

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