• Title/Summary/Keyword: $^{123}I$

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Inositol(1,4,5)triphosphate 3-Kinase의 유전자 재조합과 CCL39 Hamster Lung Fibroblasts에서 발현

  • 김재웅;최관용
    • The Korean Journal of Food And Nutrition
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    • v.9 no.2
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    • pp.123-136
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    • 1996
  • IPSKCDNA gene(1.8 kbp) encoding rat brain IP3K enzyme contained Not I restric site in open reading frame. The Not I sequence, GCGGCCGC, was converted to GCAGCCGC by site-directed mutagenesis. The mutated IP3KcDNA was digested with EcoR I and ligated with EcoR I-restricted psp72·Not2 vector. The resulting psp72 · Not2-IP3KCDNA was digested with the Not I restriction enzyme and then subcloned into the Not I -digested PZIP · NeoSV(X) mammalian expression vector. The PZIP · NeoSV(X) -IPSKCDNA was transfected into CCL39 hamster lung fibroblast cells. The efficiency of the expressed IPSKCDNA gene was significantly higher than expected generally, not only a mean 5-fold increase in the amount of enzyme, but also 16-fold increase in enzyme activity from tractsfected CCL39 cells by the method of Western blot using anti-lP3K antibodies. Both distribution of IPSK in various rat tissues and biochemical properties were discussed.

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Wet Spinning of Silk Polymer(I) -Morphological characteristics of regenerated silk fibroin filament- (실크 고분자의 습식방사(I) -재생 실크 필라멘트의 형태학적 구조 특성-)

  • 엄인철;권해용;박영환;임대우
    • Proceedings of the Korean Fiber Society Conference
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    • 2001.10a
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    • pp.123-126
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    • 2001
  • 실크는 우수한 광택, 촉감, 물성을 바탕으로 하여 인류에 있어 최고의 의류용 섬유로서 널리 이용되어 왔으며 최근에는 생체적합성 등을 활용한 의료용 및 생물공학용 소재로서 응용하고자 하는 연구가 활발히 진행 중에 있다[1-2]. 그러나 실크의 경우 누에로부터 얻어지는 천연섬유로서 합성섬유와는 달리 누에로부터 토사되면서 그 구조 및 형태가 결정되어 고정되는 단점을 안고 있어 의류용 및 산업용 분야로의 활용에 있어 한계가 있다. (중략)

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Prosodic Conditions for Epenthetic Nasals

  • Kim, Soo-Jung
    • Speech Sciences
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    • v.7 no.4
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    • pp.123-148
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    • 2000
  • This paper investigates prosodic conditions for the epenthetic /n/ in Korean. It has been claimed that an epenthetic /n/ appears across prosodic words (Han 1994, Lee 1996). However, using acoustic data as well as aerodynamic data, I argue that the epenthetic /n/ does not always surface across all prosodic words, but that its appearance is prosodically restricted. I further demonstrate that it appears only across prosodic words within an accentual phrase. This finding provides empirical support for the intonation-based model of Korean prosodic structure studies.

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Detection and Measurement of Nuclear Medicine Workers' Internal Radioactive Contamination (핵의학과 종사자의 방사성동위원소 체내오염 측정)

  • Jeong, Gyu-Hwan;Kim, Yong-Jae;Jang, Jeong-Chan;Lee, Jai-Ki
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.3
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    • pp.123-131
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    • 2009
  • Purpose: We tested a sample of nuclear medicine workers at Korean healthcare institutions for internal contamination with radioactive isotopes, measuring concentrations and evaluating doses of individual exposure. Materials and Methods: The detection and measurement was performed on urine samples collected from 25 nuclear medicine workers at three large hospitals located in Seoul. Urine samples were collected once a week, 100~200 mL samples were gathered up to 6~10 times weekly. A high-purity germanium detector was used to measure gamma radiations in urine samples for the presence of radioactive isotopes. Based on the detection results, we estimated the amounts of intake and committed effective doses using IMBA software. In cases where committed effective doses could not be adequately evaluated with IMBA software, we estimated individual committed effective doses for radionuclides with a very short half life such as $^{99m}Tc$ and $^{123}I$, using the methods recommended by International Atomic Energy Agency. Results: Radionuclides detected through the analysis of urine samples included $^{99m}Tc$, $^{123}I$, $^{131}I$ and $^{201}Tl$, as well as $^{18}F$, a nuclide used in Positron Emission Tomography examinations. The committed effective doses, calculated based on the radionuclide concentrations in urine samples, ranged from 0 to 5 mSv, but were, in the majority of cases, less than 1 mSv. The committed effective dose exceeded 1 mSv in three of the samples, and all three were workers directly handling radioactive sources. No nurses were found to have a committed effective dose in excess of 1 mSv. Conclusions: To improve the accuracy of results, it may be necessary to conduct a long-term study, performed over a time span wide enough to allow the clear determination of the influence of seasonal factors. A larger sample should also help increase the reliability of results. However, as most Korean nuclear medicine workers are currently not necessary to monitored routinely for internal contamination with radionuclides. Notwithstanding, a continuous effort is recommended to reduce any unnecessary exposure to radioactive substances, even if in inconsequential amounts, by regularly surveying workplace environments and frequently monitoring atmospheric concentrations of radionuclides.

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