• 제목/요약/키워드: $\gamma$-ray irradiation

검색결과 543건 처리시간 0.027초

Genetics, Agronomic, and Molecular Study of Leaflet Mutants in Mungbean(Vigna radiata(L.) Wilczek)

  • Soehendi, Rudy;Chanprame, Sontichai;Toojinda, Theerayut;Ngampongsai, Sumana;Srinives, Peerasak
    • Journal of Crop Science and Biotechnology
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    • 제10권3호
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    • pp.193-200
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    • 2007
  • Mungbean plants generally have a relatively close canopy, thus a large amount of self-shading can reduce yield due to poor light penetration. Modification of leaflet type can affect leaf canopy and could alter seed yield. Two multiple leaflet mutants were obtained from gamma-ray irradiation and used to study the mode of inheritance related to leaflet types and to evaluate their agronomic features. The cross between large-heptafoliate leaflet with small-pentafoliate leaflet mutants produce all $F_1$ plants with normal trifoliate leaflets. The $F_2$ plants segregated in leaflet size and leaflet number into a 9:3:3:1 ratio of large-trifoliate: large-heptafoliate: small-pentafoliate: small-heptafoliate plants, suggesting that independent loci control leaflet size and leaflet number. Regarding leaflet number, the $F_2$ population can be classified into normal-trifoliate, small-pentafoliate, large-heptafoliate, and small-heptafoliate at the dihybrid ratio of 9:3:3:1. The gene symbols $N_1,n_1$ and $N_2,n_2$ are proposed to represent leaflet number. Since no plant was found with large-pentafoliate leaflets, we hypothesize that the $N_2$ allele expresses pleiotropic effect on both leaflet number and leaflet size. Another possibility is that an additional locus with S and s alleles controls leaflet size and S is tightly linked with $N_2$. The effect of multifoliate leaflet on yield and yield components was evaluated in four mungbean families each with four leaflet isolines under three environments. Averaging across the families and environments, the normal-trifoliate and large-heptafoliate lines gave higher yield than small pentafoliate and heptafoliate ones. These two large leaflet lines also had higher leaf area per plant than the other multifoliate lines. Therefore, the mungbean lines with a greater leaf area, which were likely to intercept more sunlight, gave greater yield. Three AFLP markers that were found to be linked to number of leaflets per leaf, corresponded to the N1 allele of the smallpentafoliate parent.

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중성자 방사화분석법을 이용한 소각로 바닥재의 원소분석 (Elemental Analysis of Bottom Ash from Incinerator by Neutron Activation Analysis)

  • 문종화;강상훈;김선하;정용삼
    • 분석과학
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    • 제16권5호
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    • pp.418-425
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    • 2003
  • 도시 생활폐기물의 소각로에서 발생되는 바닥재중의 무기원소 함량을 중성자 방사화분석법으로 결정하였다. D도시 소각장에서 월별로 채취한 바닥재 시료를 5 mm 크기의 체로 거르고 오븐에 건조한 후, 막자사발로 분쇄하였다. 시료는 한국원자력연구소의 하나로 연구용 원자로에서 NAA #1 조사공을 사용하여 중성자 조사하였으며, 조사된 시료는 HPGe-감마선 분광분석장치를 사용하여 방사능을 측정하였다. 측정된 핵종의 방사능으로부터 방사능 생성식과 핵 데이타를 적용하여 As, Cr, Cu, Fe, Mn, Sb 및 Zn을 포함한 33종의 원소를 정량하였다. 또한 미국표준기술원의 인증 표준물질을 동시에 분석하여 품질관리를 하였다.

방사선(放射線) 조사(照射)가 감자 괴경(塊莖)의 맹아억제(萌芽抑制) 및 영양가(營養價)에 미치는 영향(影響) (Effects of Ionizing Radiation on Sprout Inhibition and Nutritive Value of Potato Tubers)

  • 이미순;김홍열
    • 한국식품과학회지
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    • 제4권1호
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    • pp.29-35
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    • 1972
  • 방사선 조사가 감자 괴경(塊莖)의 맹아억제(萌芽抑制) 및 영양가에 미치는 효과를 규명하기 위해서 0 krad로부터 16 krad 사이의 선량(線量)을 Irish Cobbler 품종(品種)에 처리하였다. 싹 생장량은 선량이 높아짐에 따라 감소되었고 16 krad 처리구에서 완전히 억제되었다. 이 최적선량에서 괴경(塊莖)의 중량 손실은 현저히 감소했고 전(全)저장 기간을 통해 경도(硬度)는 양호했다. 방사선 조사는 감자 괴경(塊莖)의 cooking quality에 아무런 악(惡)영향도 미치지 않았다. 저장 후에 괴경(塊莖)내의 수분함량은 싹 생장량과 역비례(逆比例)하였다. 본 실험에서 16 krad 처리구는 탄수화물 함량이 저선량(底線量)처리구보다 다소 높은 경향을 보였고, ascorbic acid는 8 및 16 krad가 무처리구 및 2 krad 보다 다량 함유되고 있음이 나타났다.

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Evaluation of the Efficacy of Zoledronic Acid and Amifostine on Radiation-induced Bone Loss in Mice

  • Kim, Jinwook;Lee, Sueun;Kang, Sohi;Moon, Changjong;Kim, Jong-Choon;Jung, Uhee;Jo, Sung-Kee;Jang, Jong-Sik;Kim, Sung-Ho
    • Journal of Radiation Protection and Research
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    • 제41권3호
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    • pp.253-259
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    • 2016
  • Background: This study investigated the effects of zoledronic acid (ZA) on radiation-induced bone loss in C3H/HeN mice. Materials and Methods: C3H/HeN mice were divided into sham control and three irradiated groups (3 Gy, gamma ray). The irradiated mice were treated for 12 weeks with vehicle, amifostine (intraperitoneal injection), or ZA (subcutaneous injection). Grip strength, uterus weight, and serum alkaline phosphatase (ALP), and tartrate-resistant acid phosphatase (TRAP) levels were measured. Tibiae were analyzed using micro-computed tomography. Results and Discussion: Treatment of ZA ($100{\mu}g{\cdot}kg^{-1}{\cdot}week^{-1}$) significantly preserved trabecular bone volume, trabecular thickness, trabecular number, trabecular separation, bone mineral density of proximal tibia metaphysic, and cortical bone volume, but did not alter the uterus weight of the mice. The administration of ZA for 12 weeks lowered serum ALP and TRAP levels in irradiated mice, suggesting that ZA can reduce the bone turnover rate in mice. No differences were apparent between the amifostine-treated group and the irradiation control group. Conclusion: The results indicate that ZA can prevent radiation-induced bone loss in mice.

고 선량율 감마선 조사 환경에서의 고성능 칼라 CCD 카메라의 관측성능 (A Monitoring Ability of the High-Performance Color CCD Camera under High Dose-Rate Gamma Ray Irradiation Environments)

  • 조재완;최영수;서용칠;정경민
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2014년도 춘계학술발표대회
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    • pp.811-814
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    • 2014
  • 일본 후쿠시마 제일 원자력발전소의 대지진/쓰나미에 이은 원자로 건물 수소폭발 사고의 수습 과정에서 사용후 핵연료 저장조에 보관되어 있는 핵연료의 안전문제가 대두되었다. 사용후 핵연료의 잔열 성분을 냉각시키고, 그리고 사용후 핵연료가 방출하는 고선량 방사선을 차폐시키기 위해서 일정 깊이 이상의 수조에 사용후 핵연료를 저장한다. 사용후 핵연료 저장조에 냉각수 공급이 중단되면, 사용후 핵연료의 고유 잔열에 의해 수조의 물이 증발하여 수위가 감소하게 된다. 계속해서 냉각수 공급이 되지 않으면, 사용후 핵연료의 잔열은 증가하게 되고, 수조의 물은 비등하여 증발은 가속화 된다. 사용후 핵연료 저장조의 수위가 고갈되면 고선량의 감마선이 방출된다. 수조의 수위가 정상적일 경우 사용후 핵연료 저장조의 공기중 감마선 선량율은 0.15mSv/h 이다. 수조의 수위가 사용후 핵연료 상부 꼭대기를 기준으로 2m, 1m, 및 0m (핵연료 노출) 로 감소하게 되면, 사용후 핵연료 저장조의 공기중 감마선 선량율은 500mSv/h, 50Sv/h, 및 5kSv/h 로, 급격히 증가한다. 본 논문에서는 사용후 핵연료 저장조 감시카메라의 관측 성능을 평가하기 위해, 고성능 칼라 CCD 카메라에 대해서 1 kGy/h 의 고선량율로 감마선 조사실험을 수행하였다. 이에 대한 실험결과를 기술한다.

Effect of Korean Red Ginseng on radiation-induced bone loss in C3H/HeN mice

  • Lee, Jin-Hee;Lee, Hae-June;Yang, Miyoung;Moon, Changjong;Kim, Jong-Choon;Bae, Chun-Sik;Jo, Sung-Kee;Jang, Jong-Sik;Kim, Sung-Ho
    • Journal of Ginseng Research
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    • 제37권4호
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    • pp.435-441
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    • 2013
  • This study investigated the effects of Korean Red Ginseng (KRG) on radiation-induced bone loss in C3H/HeN mice. C3H/HeN mice were divided into sham and irradiation (3 Gy, gamma-ray) groups. The irradiated mice were treated for 12 wk with vehicle, KRG (per os, p.o.) or KRG (intraperitoneal). Serum alkaline phosphatase (ALP), tartrate-resistant acid phosphatase, estradiol level, and biomechanical properties were measured. Tibiae were analyzed using micro-computed tomography. Treatment of KRG (p.o., 250 mg/kg of body weight/d) significantly preserved trabecular bone volume, trabecular number, structure model index, and bone mineral density of proximal tibia metaphysic, but did not alter the uterus weight of the mice. Serum ALP level was slightly reduced by KRG treatment. However, grip strength, mechanical property, and cortical bone architecture did not differ among the experimental groups. The results indicate that KRG can prevent radiation-induced bone loss in mice.

Rice blast susceptible mutants of Taebaegbyeo and genes differentially expressed in he wild type rice.

  • Lee, C. H.;C. U. Han;K. S. Jang;Park, Y. H.;H. K. Lim;Kim, J.C.;Park, G. J.;J.S. Cha;Park, J. E.
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.67.2-68
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    • 2003
  • A rice cultivar, Taebaegbyeo, is highly resistant to rice blast and moderately resistant to bacterial leaf blight (BLB) caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. To study the rice disease resistance mechanism, we generated rice deletion M3 mutants by gamma-ray irradiation. Blast and BLB responses of 16,000 M3 mutants were screened by inoculating mixtures of 4 races (KJ-201, H-1113a, KI-313, KI-409) of M. grisea and 3 Korean races of X. oryzae pv. oryzae. We selected so far 21 M3 mutants of Taebaegbyeo showing high susceptibility to the diseases. One of the mutants, KCT-6417, was susceptible to KI-1113a race of M. grisea, suggesting the deletion of a race-specific blast resistance gene in the mutant. To isolate rice genes involved in blast resistance and defense response, we take a PCR-based suppression subtractive hybridization approach using cDNAs of blast-inoculated wild type and the KCT-6417 as a tester and a driver, respectively. Genes specifically expressed in the wild type will be presented. The selected genes would give us a clue to understand mechanism for the race specific resistance and defense responses against M. grisea H-1113a in Taebaegbyeo.

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TL과 ESR 분석을 통한 일부 향신료, 장과류 및 화분가공추출물 검지 특성 연구 (Studies on the Applications of TL and ESR Methods for the Detection of Spices, Berry Fruits and Pollen Extract Product)

  • 김규헌;손진혁;강윤정;박혜영;곽지영;이재황;박용춘;조태용;이화정;이상재;한상배
    • 방사선산업학회지
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    • 제7권1호
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    • pp.69-74
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    • 2013
  • This study examined radiation detection characteristics of spices (cumin, caper and turmeric), other small fruits (hut-gae berry and boxthorn), and pollen extract product. Each samples were irradiated at dose of 1, 5, and 10 kGy and analyzed by the thermoluminescence (TL) and electron spin resonance (ESR) methods. To compare between non-irradiated and irradiated food, all samples were irradiated with $^{60}Co$ gamma-ray source. In TL analysis, most of samples could be applied to detect irradiated foods because of showing TL ratio above 0.1. The glow curves examined by TL method were estimated in the range of $150{\sim}250^{\circ}C$ in irradiated samples. In ESR measurements, the intensity of ESR signal (single-line) increased as the increase of irradiation dose. In particular, the specific ESR signals of irradiation-induced radical were detected in hut-gae berry and pollen extract product. As a results, it is considered that TL and ESR methods can be used to detect both hut-gae berry and pollen extract product. But cumin, caper, turmeric and boxthorn irradiated with gamma ray could be detected only by TL method. It is concluded that TL and ESR methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.

MC-50 싸이클로트론을 이용한 $^{123}I$ 제법 연구 (The Development of Iodine-123 with MC-50 Cyclotron)

  • 서용섭;양승대;전권수;이종두;한현수
    • 대한핵의학회지
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    • 제25권2호
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    • pp.286-293
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    • 1991
  • $^{123}I$, which is applied for the thyroid and other in vivo kinetic study, has a special role in life sciences. The 159 KeV $\gamma-ray$ from $^{123}I$ is almost ideally appropriate for the current imaging instrumentation. Its decay mode (electron capture) and short half-life (13.3 hr) reduced the burden of radiation dose to the patients, and its chemical property makes it easy to synthesize the labelling compounds. In this experiment, the production of $^{123}I$ via the nuclear reaction $^{124}Te(p,2n)^{123}I$ with 28 MeV protons was sutdied. $TeO_2$ is used as a target material, because it has good physical properties. The target was prepared with $TeO_2$ powder and was molten into a ellipsoidal cavity (a=14 mm, b=10 mm, $270.8mg/cm^2$ thick) of pure platinum. The irradiation was carried out in the external proton beam with incident energies range from 28 MeV to 22 MeV, and current was $30{\mu}A$. The loss of $TeO_2$ target was significantly reduced by using $4\pi-cooling$ system in irradiation. The dry distillation method was adopted for the separation of $^{123}I$ from irradiated target, and when it was kept 5 minutes at $780^{\circ}C$, its result was quantitative. The loss of the target material $(TeO_2)$ was below 0.2% for each production run and $^{123}I$ from the dry distillation apparatus was captured with 0.01 N NaOH in $Na^{123}I$ form, then the pH of the solution was adjusted to $7.5\sim9.0$ with HC1/NaOH. The $Na^{123}I$ solution was passed through $0.2{\mu}m$ membrane filter, and sterilized under high pressure and temperature for 30 minutes. The production of $^{123}I$ is acceptable for clinical application based on the quality of USP XXI.

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자연방사선 고준위 지역 사육 소의 림프구 미소핵 발생 평가 (Incidence of Micronuclei in Lymphocytes of Cattle in the High Background Radiation Area)

  • 이해준;강창모;김세라;문창종;김종춘;김일화;조성기;장종식;김성호
    • Toxicological Research
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    • 제22권4호
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    • pp.417-422
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    • 2006
  • Cytogenetic and hematological analysis was performed in peripheral blood obtained from cattle bred in the high background radiation areas (HBRA, Goesan-gun, Cheongwon-gun, Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in 3 cattle. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of $y=(0.0583{\pm}0.0137)D+(0.0366{\pm}0.0081)D^2+(0.0093{\pm}0.0015)$ (y=number of MN/CB cells and D=irradiation dose in Gy). MN rates per 1,000 CB lymphocytes of cattle from the Goesan-gun, Cheongwon-gun, Boeun-gun and the control area were $6.50{\pm}2.72,\;9.00{\pm}4.50,\;10.89{\pm}4.23\;and\;9.60{\pm}4.70$, respectively. The MN frequencies of CB lymphocytes from cattle bred in 4 areas mean that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the cattle were bred in the HBRA or the control area.