Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

Incidence of Micronuclei in Lymphocytes of Cattle in the High Background Radiation Area

자연방사선 고준위 지역 사육 소의 림프구 미소핵 발생 평가

  • Lee, Hae-June (Korea Institute of Radiological & Medical Science) ;
  • Kang, Chang-Mo (Korea Institute of Radiological & Medical Science) ;
  • Kim, Se-Ra (College of Veterinary Medicine, Chonnam National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Ill-Hwa (College of Veterinary Medicine, Chungbuk National University) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute, KAERI) ;
  • Jang, Jong-Sik (Department of Animal Science, Sangju National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cytogenetic and hematological analysis was performed in peripheral blood obtained from cattle bred in the high background radiation areas (HBRA, Goesan-gun, Cheongwon-gun, Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in 3 cattle. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of $y=(0.0583{\pm}0.0137)D+(0.0366{\pm}0.0081)D^2+(0.0093{\pm}0.0015)$ (y=number of MN/CB cells and D=irradiation dose in Gy). MN rates per 1,000 CB lymphocytes of cattle from the Goesan-gun, Cheongwon-gun, Boeun-gun and the control area were $6.50{\pm}2.72,\;9.00{\pm}4.50,\;10.89{\pm}4.23\;and\;9.60{\pm}4.70$, respectively. The MN frequencies of CB lymphocytes from cattle bred in 4 areas mean that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the cattle were bred in the HBRA or the control area.

Keywords

References

  1. Almassy, Z., Krepinsky, A.B., Bianco, A. and Koteles, G.J. (1987): The present state and perspectives of micronucleus assay in radiation protection. A review. Int. J. Rad. Appl. Instrum. [A], 38, 241-249 https://doi.org/10.1016/0883-2889(87)90033-5
  2. Backer, L.C., Grindem, C.B., Corbett, W.T., Cullins, L. and Hunter, J.L. (2001): Pet dogs as sentinels for environmental contamination. Sci. Total Environ., 274, 161-169 https://doi.org/10.1016/S0048-9697(01)00740-9
  3. Catena, C., Asprea, L., Carta, S., Tortora, G., Conti, D., Parasacchi, P. and Righi, E. (1997): Dose-response of Xirradiated human and equine lymphocytes. Mutat. Res., 373, 9-16 https://doi.org/10.1016/S0027-5107(96)00178-9
  4. Chung, H.W., Kim, S.Y., Sohn, E.H. and Ha, S.W. (2000): Analysis of chromosome aberrations in nuclear-powerplant workers considering the lifetime of lymphocytes. Int. J. Radiat. Biol., 76, 923-927 https://doi.org/10.1080/09553000050050936
  5. Ghiassi-Nejad, M., Zakeri, F., Assaei, R.G. and Kariminia, A. (2004): Long-term immune and cytogenetic effects of high level natural radiation on Ramsar inhabitants in Iran. J. Environ. Radioact., 74, 107-116 https://doi.org/10.1016/j.jenvrad.2003.12.001
  6. Hayata, I., Wang, C., Zhang, W., Chen, D., Minamihisamatsu, M., Morishima, H., Yuan, Y., Wei, L. and Sugahara, T. (2000): Chromosome translocation in residents of the high background radiation areas in southern China. J. Radiat. Res. (Tokyo), 41 Suppl, 69-74 https://doi.org/10.1269/jrr.41.S69
  7. He, J.L., Jin, H.Y., Jin, L.F. and Gao, S.Y. (2000): Monitoring of human exposure to radiation with the binucleated lymphocyte micronucleus assay. Biomed. Environ. Sci., 13, 32-36
  8. Ishihara, T. and Sasaki, M. (1983): Radiation-induced chromosome damage in man. Alan R. Liss, Inc. New York, pp. 561-583
  9. Jiang, T., Hayata, I., Wang, C., Nakai, S., Yao, S., Yuan, Y., Dai, L., Liu, Q., Chen, D., Wei, L. and Sugahara, T. (2000): Dose-effect relationship of dicentric and ring chromosomes in lymphocytes of individuals living in the high background radiation areas in China. J. Radiat. Res.(Tokyo), 41 Suppl, 63-68
  10. Kim, S.R., Kim, T.H., Ryu, S.Y., Lee, H.J., Oh, H., Jo, S.K., Oh, K.S., Park, I.C., Kim, J.C., Kang, C.M. and Kim, S.H. (2003): Measurement of micronuclei by cytokinesis-block method in human, cattle, goat, pig, rabbit, chicken and fish peripheral blood lymphocytes irradiated in vitro with gamma radiation. In Vivo, 17, 433-438
  11. Muller, W.U. and Streffer, C. (1991): Biological indicators for radiation damage. Int. J. Radiat. Biol., 59, 863-873 https://doi.org/10.1080/09553009114550771
  12. Ramalho, A., Sunjevaric, I. and Natarajan, A.T. (1988): Use of the frequencies of micronuclei as quantitative indicators of X-ray-induced chromosomal aberrations in human peripheral blood lymphocytes: comparison of two methods. Mutat. Res., 207, 141-146 https://doi.org/10.1016/0165-7992(88)90078-4
  13. Scarfi, M.R., Lioi, M.B., Di Berardino, D., Zeni, O., Coviello, A.M. and Matassino, D. (1993): Measurement of micronuclei by cytokinesis-block method in bovine lymphocytes. Mutat. Res., 289, 291-295 https://doi.org/10.1016/0027-5107(93)90080-Y
  14. Shliakhtenok, A.S. (2003): Dynamics of $^{134+137}Cs$ accumulation in insects inhabiting the 30-kilometer zone of Chernobyl Nuclear Power Station. Radiats. Biol. Radioecol., 43, 93-96
  15. Spirin, E.V. (2002): Reconstruction of I-131 in milk and exposure doses to the thyroid gland of cattle after the Chernobyl AES. Radiats. Biol. Radioecol., 42, 564-568
  16. Streffer, C., Müller, W.U., Kryscio, A. and Böcker, W. (1998): Micronuclei-biological indicator for retrospective dosimetry after exposure to ionizing radiation. Mutat. Res., 404, 101-105 https://doi.org/10.1016/S0027-5107(98)00100-6
  17. Sutiakova, I., Sulik, E., Rimkova, S., Sakalikova, A. and Sutiak, V. (2001): Micronucleus frequency in cytokinesisblocked bovine lymphocytes from regions with different pollution levels in Slovakia. Bull. Environ. Contam. Toxicol., 66, 449-455 https://doi.org/10.1007/s00128-001-0027-9
  18. Tempel, K. (1997): Chernobyl and its consequences-some veterinary medical points of view. Tierarztl. Prax. Ausg. G. Grosstiere. Nutztiere., 25, 401-405
  19. Thierens, H., Vral, A., Barbe, M., Aousalah, B. and De Ridder, L. (1999): A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay. Mutat. Res., 445, 105-111 https://doi.org/10.1016/S1383-5718(99)00134-5
  20. Thierens, H., Vral, A., Barbe, M., Meijlaers, M., Baeyens, A. and Ridder, L.D. (2002): Chromosomal radiosensitivity study of temporary nuclear workers and the support of the adaptive response induced by occupational exposure. Int. J. Radiat. Biol., 78, 1117-1126 https://doi.org/10.1080/0955300021000034710
  21. Thomson, E.J. and Perry, P.E. (1988): The identification of micronucleated chromosomes: a possible assay for aneuploidy. Mutagenesis, 3, 415-418 https://doi.org/10.1093/mutage/3.5.415
  22. Ulsh, B., Hinton, T.G., Congdon, J.D., Dugan, L.C., Whicker, F.W. and Bedford, J.S. (2003): Environmental biodosimetry: a biologically relevant tool for ecological risk assessment and biomonitoring. J. Environ. Radioact., 66, 121-139 https://doi.org/10.1016/S0265-931X(02)00119-4
  23. Vandecasteele, C.M. (2004): Environmental monitoring and radioecology: a necessary synergy. J. Environ. Radioact., 72, 17-23 https://doi.org/10.1016/S0265-931X(03)00181-4
  24. Zhang, W., Wang, C., Chen, D., Minamihisamatsu, M., Morishima, H., Yuan, Y., Wei, L., Sugahara, T. and Hayata, I. (2003): Imperceptible effect of radiation based on stable type chromosome aberrations accumulated in the lymphocytes of residents in the high background radiation area in China. J. Radiat. Res. (Tokyo), 44, 69-74 https://doi.org/10.1269/jrr.44.69
  25. 김세라, 강창모, 김성호 (2004): 세포질 분열 차단 림프구를 이용한 울진원자력발전소 주변 소의 미소핵 발생 평가. 대한수의학회지, 44, 343-348
  26. 김세라, 김성호 (2003): 림프구 미소핵 측정법을 이용한 원자력발전소 주변 소의 이상산에 대한 방사선생물학적 평가. 한국임상수의학회지, 20, 364-368
  27. 안병균 (2002): 충북지역의 환경방사선의 분석. 산업과학기술연구소 논문집, 16, 81-85
  28. 안병균 (1999): 중부지역 환경방사선의 분석. 과학교육연구논총, 15, 37-42
  29. 안윤옥 (2003): 원전 주변지역 역학조사 연구. 과학기술부 연구보고서, 288-301