• Title/Summary/Keyword: $\gamma$상

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Induction of Mutants by Irradiation of $\gamma$-Ray on In vitro Shoots of Persimmon (기내$\gamma$-선 처리에 의한 감(Diospyros kaki Thunb.) 돌연변이 유기)

  • 고갑천
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.2
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    • pp.143-148
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    • 2000
  • This study was carried out to know the optimum dose of gamma-ray for the induction of mutation in vitro and the characteristics of the mutants induced by gamma-ray in persimmon (Diospyros kaki Thunb.). The LD50 (50% lethal dose) for in vitro shoots of the cultivar, Nishimurawase was between 1 krad and 2 krad and about 1 krad for the cultivar, Ichikikeijiro. As the dose of gamma-ray increased, the length of shoots decreased and necrosis of buds increased. For the cultivar, Nishmurawase, 37.5∼58.3% shoots rooted and the rooting rate and the number of roots per shoot was low in high gamma-ray. The irradiated young plants which were grown in the growth cabinet for 6 weeks were shorter in shoot length and had more branches than non-irradiated plants. The survival rate of irradiated plants grown in the green house for 3 months was 33%, while 77% for control plants.

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Effect of glutamic acid and its ${\gamma}-derivatives$ on the production of ${\delta}-aminolevulinic{\;}acid$ by Rhodobacter sphaeroides (Rhodobacter sphaeroides에 의한 ${\delta}-aminolevulinic{\;}acid$생산에 있어서 glutamic acid 및 감마 유도체의 영향)

  • Choi, Kyung-Min;Lim, Wang-Jin;Hwang, Se-Young
    • Applied Biological Chemistry
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    • v.36 no.3
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    • pp.184-190
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    • 1993
  • The effect of ${\delta}-aminolevulinic\;acid$ (ALA) biosynthetic precursors and related compounds on the ALA productivity from a strain of Rhodobacter sphaeroides has been examined in vivo and in vitro systems. The relative ratios of ALA productivities by $C_{4}$- pathway to that by $C_{5}$-pathway in vivo and in vitro systems were 0.78 and 1.37, respectively. Although the expression rates of $C_{4}-$ and $C_{5}-pathways$ in cell-free systems prepared after precursors supplemented cultivations were increased 1.35 and 1.52 folds, respectively, the rate increase of $C_{4}-pathway$ was accompanied by the rate decrease of the $C_{5}-pathways$, and vice versa, as that the rates of both $C_{4}-$ and $C_{5}-pathways$ were lowered to be 0.91, 0.83, respectively. The order of cellular uptake rates of ${\gamma}-glutamyl$ derivatives relative to that found with L-glutamic acid were shown to be D-glutamic acid, 0.55: D-glutamine, 0.5: L-glutamine, 0.4: ${\gamma}-L-glutamyl$ ethylester, 0.3: GSH and Glu-pNA, 0. L and D configurations of glutamine were indicated as better substrates in vivo for ALA yields than those of glutamic acid, respectively.

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Analysis on Thermal Degradation of Poly($\gamma$-glutamic acid) Sodium Salt by means of Light Scattering and Viscometry (광산란과 점성도법에 의한 폴리감마글루탐산 나트륨 염의 열분해 분석)

  • Park, Il-Hyun;Eom, Hyo-Sang;Kwon, Hyo-Lee
    • Polymer(Korea)
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    • v.33 no.5
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    • pp.501-508
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    • 2009
  • The thermal degradation experiment of sodium salt of poly (${\gamma}$-glutamic acid) (PGGNa) has been carried out in both its solid phase and solution phase at the range of $57{\sim}120^{\circ}C$ and their molecular weight decreasing effect was analyzed as a function of time by means of viscometry and light scattering. Based on the solid phase degradation results, it was supposed that the bond scission rate in a polymer chain kept constant and that the bond scission was occurred on a randomly located position in a polymer chain. For the degradation in solution phase, it was also found that all data at various temperatures were dropped on a single master curve when the reduced time $t/t^*$ was used in the plot of the reciprocal intrinsic viscosity (or molecular weight). This degradation curve in solution phase could be expressed as the sum of a single exponential and a linear equation and especially, the single exponential character appeared only at the beginning stage. The activation energy was measured as $107{\sim}115$ kJ/mol in this study and agreed with the literature values.

Studies on the Safety of Recombinant Bovine Somatotropin in Dairy Cow : Effects of ${\gamma}$BST on Hematologic and Blood Chemical Values in Dairy Cow (${\gamma}$BST의 젖소에 대한 안전성 연구 II. 성장호르몬이 젖소의 혈액상 및 혈액화학치에 미치는 영향)

  • Lee Mun-Han;Jin Young-Wha;Lee Chang-Woo
    • Journal of Veterinary Clinics
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    • v.8 no.2
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    • pp.157-170
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    • 1991
  • Effects of recombinant bovine somatotropln(${\gamma}$BST) on hematologie and blood chemical values were investigated in twenty-five multiparous Holstein dairy cows. Recombinant BST was administered by two different routes ; intramusculary(12.5mg and 25mg/day) and subcutaneously(500mg and 750mg) in sustained-release vehicle every 2 weeks beginning 4 weeks postpartum and continuing for 7 months. Whole blood and serum samples were collected 0, 1, 2, 3, 5, and 7 months after beginning of treatments from control and ${\gamma}$BST-administered groups. Hematologic values including RBC, PCV, HB, MCH, MCHC, WBC and differential counts of treatment groups receiving ${\gamma}$BST were similiar to those of control group. Blood chemical values observed were total protein, albumin, A/G ratio, glucose, cholesterol, Ca, Pi, Ca/pi ratio, total bilirubin, creatinine, BUN, alkaline phosphatase, lactate dehydrogenase, alanine aminotransferase and aspartate aminotransferase. There were no significant differences in blood chemical values of cows administered with ${\gamma}$BST from those of control. Although some blood chemical values were fluctuated at a certain observation period, they were remained within the normal physiological ranges. It is concluded from the observations of these experiments that the dose and dosage froms of ${\gamma}$BST employed in this work might not affect hematologic and blood chemical values in dairy cows under the normal sanitary condition and adequate nutritional balance.

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1,1-Difluoroethane Synthesis from Acetylene over Fluorinated γ-Al2O3 (불화된 γ-Al2O3상에서 아세틸렌으로부터 1,1-difluoroethane의 합성)

  • Lee, Youn-Woo;Lee, Kyong-Hwan;Lim, Jong Sung;Kim, Jae-Duck;Lee, Youn Yong
    • Applied Chemistry for Engineering
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    • v.9 no.5
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    • pp.629-633
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    • 1998
  • The synthesis of 1,1-difluoroethane from acetylene as a function of HF/acetylene ratio, contact time and reaction temperature was studied on a fluorinated ${\gamma}-Al_2O_3$. The fluorination of ${\gamma}-Al_2O_3$ was treated with pure HF gas at high temperature. The crystallinity, the porosity, and the acid properties of the prepared samples were examined using XRD, the nitrogen adsorption, pyridine-IR and ammonia-TPD respectively. The activity was enhanced by further fluorination of alumina. The fraction of 1,1-difluoroethane was obtained above 90% at reaction temperature of about $200^{\circ}C$. The ratio of 1,1-difluoroethane to vinylfluoride over fluorinated ${\gamma}-Al_2O_3$ catalyst was increased with the mole ratio of HF/acetylene and contact time, and was found to be the highest ratio at reaction temperature of $200^{\circ}C$.

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The development of th gamma-ray imaging and operation algorithm for the gamma-ray detection system (감마선 탐지장치의 감마선 영상화 및 운용 알고리즘 개발)

  • Song, Kun-young;Hwang, Young-gwan;Lee, Nam-ho;Yuk, Young-ho
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.10a
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    • pp.942-943
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    • 2016
  • Stereo gamma ray detection system generates a two-dimensional image of the gamma ray by using the position values and the gamma ray signal. And the device will overlap with the visible light image shows the actual distribution of the gamma-ray space. The gamma ray detection device is a stereo configuration to a motion controller for controlling the signal measurement unit and the position detection portion for detecting the detection portion and the gamma-ray signal comprising a gamma-ray detection sensor. In this paper, we developed a system operation management algorithm for each module individually configured efficiently. We confirmed the imaged and distribution information output for the gamma rays from gamma-ray irradiation test site by using these results.

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Study of Mössbauer Spectroscopy for Iron Oxides Synthesized by Pulsed Wire Evaporation (PEW) (전기선폭발법으로 제조된 철산화물의 뫼스바우어분광연구)

  • Uhm, Young Rang
    • Journal of the Korean Magnetics Society
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    • v.24 no.5
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    • pp.135-139
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    • 2014
  • Iron-oxide nanopowders were synthesized by a pulsed wire evaporation (PWE) in various ambient gas conditions. SEM measurement indicates that the spherical iron nanoparticles are about 50 nm in diameter. The phase analysis for the produced iron-oxide powders was systematically investigated by using $M\ddot{o}ssbauer$ spectra and the results show that classified phases of $Fe_2O_3$ and $Fe_3O_4$ can be controlled by regulating the oxygen concentration in the mixed gas during the PWE process. A quadrupole line on the center of $M\ddot{o}ssbauer$ spectrum represents the superparamagnetic phase of 12 % from ${\gamma}-Fe_2O_3$ phase.

Crystal structures and magnetic properties of Mn-Al-M (M=Cu, Fe) alloys (Mn-Al-M(M=Cu, Fe) 합금계의 결정구조 및 자기적 성질)

  • Choe, Won-Gyu;Go, Gwan-Yeong;Yun, Seok-Gil
    • Korean Journal of Materials Research
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    • v.5 no.1
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    • pp.22-35
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    • 1995
  • In this study, crystal structures and magnetic properties of as-ast, annealed and rapidly solidified Mn-A1-M( M=Cu, Fe) alloys have been investigated. In $Mn_{0.56}Al_{0.44}$ alloys, the largest fraction of $\tau$ phase and values of magnetic properties was obtained in Mnl, i6Alo or alloy. And this alloy was used as the basic composition. In $Mn_{0.56-X}M_{X}Al_{0.44}$ alloys, when annealed, $\tau$- and $\beta$-Mn phase appeared at x< 0.08, $\tau$- and $\kappa$ phase at 0.10 $\leq x \leq$ 0.12 and $\kappa$- phase only at 0.15 $\leq x \leq$0.20 . When rapidly solidified, specimens showed similar phases as when annealed except that $\varepsilon$ phase appeared at x=0.04. In Mnu FexAlo 44 alloys, asyast specimens showed $\tau$-, $\beta$-Mn and $\gamma_2$- phase at x<0.08 and K and $\beta$-Mn phase at x>0.10. When rapidly solidified, Mn-Fe-Al specimens showed $\varepsilon$-, $\gamma_2$- and small amount of $\tau$- and $\kappa$ phase at x<0.08 and $\kappa$- phase only at 0.$\leq x \leq$0.20. All the alloys investigated were ferromagnetic. The Curie temperature of annealed specimens and rapidly solidified of Mno 5sAlu 44 alloy were -650K and -644K. Spontaneous magnetization( UII of annealed and rapidly solidified specimens were 40-45 (emu/g) and 50-52(emu/g), respectively. Remanent (M,) to saturation magnetization( Ms) ratio was -0.7. M, of rapidly solidified specimen was about 48(emu/g). Magnetic properties of $Mn_{0.56}Al_{0.44}$ alloys were found to be determined by the relative fraction of ferromagnetic r- and K- phase. When M= Cu and x=0.15, maximum as($\sigma_{0.0}$) was obtained by about 64.3 emu/g), and when M=Fe and x=0.15, 66.4( emu/g). The Curie temperature decreased as x increased.

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Study the Analysis of Comparison with AROI and MROI Mode in Gated Cardiac Blood Pool Scan (게이트심장혈액풀 스캔에서 자동 관심영역 설정과 수동 관심영역 설정 모드의 비교 분석에 관한 고찰)

  • Kim, Jung-Yul;Kang, Chun-Koo;Kim, Yung-Jae;Park, Hoon-Hee;Kim, Jae-Sam;Lee, Chang-Ho
    • The Korean Journal of Nuclear Medicine Technology
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    • v.12 no.3
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    • pp.222-228
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    • 2008
  • Purpose: The objectives of this study were to compare the left ventricle ejection fraction (LVEF) from gated cardiac blood pool scan (GCBP) for analysis auto-drawing region of interest mode (AROI) and manual-drawing region of interest mode (MROI), respectively. To evaluation the relationships between values produced by both ROI modes. Materials and Methods: Gated cardiac blood pool scan using in vivo method Tc-99m Red Blood Cell were performed for 33 patients (mean age: $53.2{\pm}13.2\;y$) with objective of chemotherapy using single head gamma camera (ADAC Laboratories, Milpitas, CA). Left ventricular ejection fraction was automatically and manually measured, respectively. Results: There was significant difference statistically between AROI and MROI ($LVEF^{AROI}$: $71.4{\pm}12.4%$ vs. $LVEF^{MROI}$: $65.8{\pm}5.9%$, p=0.003). Intra-observer agreements in AROI was higher than MROI ($\gamma^{AROI}=0.964$, Cronbach's $\alpha^{AROI}=0.986$ vs. $\gamma^{MROI}=0.793$, Cronbach's $\alpha^{MROI}=0.911$), either. Additionally, there was no significant difference statistically at best septal view (${\Delta}LVEF^{BSV}=0.7{\pm}2.3%$, p=0.233), however statistically significant difference was found at badly separated septal view (${\Delta}LVEF=10.9{\pm}11.4%$, p=0.001). Moreover, Intra-observer agreements in best septal view was higher than badly separated septal view ($\gamma^{BSV}=0.939$, Cronbach's $\alpha^{BSV}=0.978$; $\gamma=0.948$, Cronbach's $\alpha=0.981$ at AROI, $\gamma^{BSV}=0.836$, Cronbach's $\alpha^{BSV}=0.936$; $\gamma=0.748$, Cronbach's $\alpha=0.888$ at MROI). Conclusion: When best septal view was acquired, LVEF by AROI and MROI indicated not different. Comparing Intra-observer agreements with AROI and MROI, the AROI tended to show higher. Therefore, it is considered that the AROI than MROI is valuable in reproducibility and objective when ROI analysis by acquire left ventricular of best septal view.

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Plasmid DNA damage by neutron and ${\gamma}$-ray in the presence of BSH (BSH 존재시 중성자 및 ${\gamma}$-ray 조사에 따른 plasmid DNA의 손상)

  • Chun, Ki-Jung;Seo, Won-Sook
    • Journal of Radiation Protection and Research
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    • v.31 no.2
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    • pp.65-68
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    • 2006
  • In this study, the extent of plasmid DNA damage was observed according to concentration of BSH(Boron Sulfhydryl Hydride) and irradiation doses of neutron and ${\gamma}$-ray. The plasmid used was both pBR 322 (2870 bp) and ${\Phi}X174$ RF(5386 bp) DNA. Plasmid DNA damage by irradiation in the presence of BSH was analyzed by agarose gel electrophoresis. In the neutron experiment, DNA damage of both plasmid DNAs was increased according to increasing the concentration of BSH and neutron doses. But in the ${\gamma}$-ray experiment, there appeared no dose dependency as compared to the neutron experiment. The extent of the plasmid DNA damage in the presence of BSH was somewhat different according to irradiation by neutron or ${\gamma}$-ray.