• 약학회지
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• 제37권2호
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• pp.187-192
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• 1993

Approximately 1% of intestinal bacteria of human and rats was alkalotolerant. Among these bacteria of human, bacteria producing $\beta$-glucosidase, $\beta$-glucuronidase and sulfotransferase were 40%, 4% and 0%, respectively. Among alkalotolerant intestinal bacteria of rats, bacteria producing, these enzymes were 70%, 8% and 0%, respectively. $\beta$-Glucosidase and $\beta$-glucuronidase of alkalotolerant intestinal bacteria of human and rat were induced by the medium of high pH: these enzymes activities were increased by elevating pH of the medium, but the growths were not changed. The enzyme activities at the medium of pH 7 were about ten-fold higher than those at the medium of pH 6.

• Food Science and Biotechnology
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• 제17권5호
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• pp.1021-1024
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• 2008

Genistein, one of the isoflavones in doenjang, is generally known to prevent various cancers, osteoporosis, climacterium, and menopause symptoms, and has better bioavailability and healthful physiological effects than its glucoside, genistin. In both traditional and commercial doenjangs, genistein content ranged from 370 to 1,510 mg/kg, however, significant amounts of genistin also existed at the level of 190 to 350 mg/kg. After treating with corn $\beta$-glucosidase, over 84% of genistin in doenjang was converted to genistein. However, physiochemical characteristics such as pH, viscosity, 2-thiobarbituric acid (TBA) value, and color were not changed significantly after corn $\beta$-glucosidase treatments. Therefore, this study shows that the improved doenjang with the increased genistein content can be produced using corn $\beta$-glucosidase.

• 한국식품과학회지
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• 제44권3호
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• pp.331-336
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• 2012

본 연구에서는, 치자 열매를 항염증 소재로 선택하여, 치자 추출물(GFE)에 ${\beta}$-glucosidase를 처리함으로써 항염증 활성을 증진하고자 하였다. 항염증 활성의 비교를 위해 추출물과 LPS를 함께 처리하여 RAW264.7 세포가 생산하는 NO의 양을 측정하였다. Lactic acid bacteria, 식물 그리고 곰팡이 유래 ${\beta}$-glucosidase를 처리한 결과, 곰팡이(A. niger, A. fumigatus)에서 유래된 효소를 처리하는 것이 GFE의 NO생산 억제활성 증진에 가장 효과적이었다. 그리고 GFE에 대한 A. niger 유래 ${\beta}$-glucosidase의 최적 처리조건은 pH 4.6, $45^{\circ}C$ 그리고 20 U/mL의 효소 농도로 24 h 반응시키는 것이었다. 이와 같은 조건으로 GFE에 효소를 처리한 GFAN은 GFE와 유사한 세포 생존율을 나타냈고, 세포독성은 나타나지 않았다. 또한 GFAN의 처리에 의하여 RAW264.7 세포의염증성 cytokine생산, COX-2생산 그리고 NF-${\kappa}B$활성이 현저히 줄어들었다. 본 연구에서 GFAN의 항염증 활성 증진은 치자의 성분 중 주로 geniposide가 ${\beta}$-glucosidase처리에 의하여 genipin으로 전환되었기 때문이라 생각된다. 이상의 결과는 ${\beta}$-glucosidase를 처리한 치자 추출물(GFE)을 이용하여 안전하고 효과적인 항염증 기능성 식품 소재 개발이 가능함을 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.441-448
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• 1998

A cellobiose-utilizing recombinant yeast having $\beta$-glucosidase activity was developed for ethanol production from a mixture of glucose and cellobiose. Using $\delta$-sequences of Tyl transposon of yeast as target sites for homologous recombination, a heterologous gene of $\beta$-glucosidase was integrated into the chromosome of Saccharomyces cerevisiae. The $\delta$-integrated recombinant yeast, Saccharomyces cerevisiae L2612 (Pb-BGL), showed perfect mitotic stability even in nonselective media and showed ca. 1.5 fold higher $\beta$-glucosidase activity than the recombinant yeast harboring the $2\mu$-based plasmid vector system. A mathematical model was developed to describe the $\beta$-glucosidase formation and ethanol production from the Saccharomyces cerevisiae L2612 ($p\delta-BGL$). The model newly described that the heterologous $\beta$-glucosidase production mediated by ADH1 promoter is regulated by glucose and repressed by ethanol.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.788-794
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• 2014

An extracellular ${\beta}$-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 ${\beta}$-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at $65^{\circ}C$ and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The ${\beta}$-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 ${\beta}$-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.

• Journal of the Korean Wood Science and Technology
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• 제35권5호
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• pp.100-108
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• 2007

$\beta$-Glucosidase from Laetiporus sulphureus among the enzymes related to lignocellulosic biomass degradation to sugars for using alternative bioethanol production was characterized. The highest activity of $\beta$-glucosidase was obtained on cellobiose at shaking culture. For the characterization and purification of $\beta$-glucosidase culture solution was concentrated and then purified by FPLC using ion exchange and size exclusion column. According to the results of SDS-PAGE, native PAGE and microfluidic system of purified enzyme, protein band was observed at about 132 kDa. Optimal pH and temperature of purified $\beta$-glucosi-dase were 5.0 and $60^{\circ}C$, respectively. In the kinetic properties of $\beta$-glucosidase on various substrates such as sophorose, gentiobiose and cellobiose, $K_m$ was 0.81, 1.07 and 1.70 mM, respectively.

• 한국미생물·생명공학회지
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• 제20권2호
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• pp.164-168
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• 1992

Cellulomonas sp.YE-5가 생산하는 cellulase를 분리, 정제하여 효소의 특성을 알아보았다. Avicelase, CMCase, $\beta$-glucosidase의 반응 최적온도은 각각 40, 45, $40^{\circ}C$이었고, 반은 최적 pH는 5.5, 6.0 그리고 6.0이었다. 효소의 열안정성은 30~$70^{\circ}C$에서 6시간 처리하였을 때 avicelase와 $\beta$-glucosidase는 $50^{\circ}C$ 이상에서 거의 실활하였고, CMCase는 $50^{\circ}C$에서 약 40%의 활성을 유지하였다. 효소의 안정성에 미치는 pH의 영향은 $25^{\circ}C$에서 24시간 처리하였을 때 avicelase와 CMCase는 PH 5.0~9.0 사이에서 안정하였으며, $\beta$-glucosidase는 pH 5.0~8.0 사이에서 안정하였다.

• Journal of Microbiology and Biotechnology
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• 제23권11호
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• pp.1577-1585
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• 2013

Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of ${\beta}$-glucosidase. In this study, three ${\beta}$-glucosidases from different organisms were respectively expressed in Saccharomyces cerevisiae and the ${\beta}$-glucosidase from Saccharomycopsis fibuligera showed the best activity (5.2 U/ml). The recombinant strain with S. fibuligera ${\beta}$-glucosidase could metabolize cellobiose with a specific growth rate similar to the control strain in glucose. This recombinant strain showed higher hydrolysis efficiency in the cellulose simultaneous saccharification and fermentation, when using the Trichoderma reesei cellulase, which is short of the ${\beta}$-glucosidase activity. The final ethanol concentration was 110% (using Avicel) and 89% (using acid-pretreated corncob) higher than the control strain. These results demonstrated the effect of ${\beta}$-glucosidase secretion in the recombinant S. cerevisiae for enhancing cellulosic ethanol conversion.

• 미생물학회지
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• 제35권1호
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• pp.35-40
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• 1999

소양호에서 식물 플랑크톤이 분비하는 유기물의 분자량 크이게 따른 세균수와 활성의 변화를 측정하였다. 소양호 상걸리 유역에서 여름철에 채수한 물을 tangential flow ultrafiltration 으로 용존 유기물질을 100,000 nMW~0.1$\mu\textrm{m}$, 10,000 nMW~100,000 nMW와 1,000 nMW~10000 nMW의 3개 fraction으로 구분하였고, 여기에 호숫물을 접종하여, 세균수와 $\beta$-glucosidase 의 변화를 측정하였다. 배양기간 동안에 나타난 총세균수는 24시간까지 급격히 증가한 후 점차 안정적으로 변하는 전형적인 성장곡선을 나타내었으며, DOC 농도와 종류가 달랐음에도 , $1.2{\times}10^{7}$ cells $ml^(-1)$범위였고, 저분자 fraction에 비해 최고 1,000배 이상 높았다. 즉 10,000 nMW 이상의 고분자 용존 유기물질은 $\beta$-glucosidase의 유도체로 작용하며, 저분자 용존 유기물질은 $\beta$-glucosidase 활성도를 높이지 못하는 것으로 확인되었다.

• 한국식품과학회지
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• 제31권6호
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• pp.1405-1409
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• 1999

본 연구는 콩, 메주, 된장에서의 isoflavones의 aglycones % 차이와 ${\beta}-glucosidase$의 활성을 살펴보기 위하여 수행되었다. Isoflavones 중 체내 이용률이 높은 aglycones %는 콩보다는 메주, 된장에서 높게 나타났다. 콩의 경우 유리 daidzein이 106 mg/kg, 총 dai-dzein은 406 mg/kg으로 aglycones 비율이 26.03%이었고 유리 genistein이 95 mg/kg, 총 genistein은 484 mg/kg으로 aglycones 비율이 19.49%이었으며 daidzein/genistein ratio는 0.85이었다. 메주는 유리 daidzein이 269 mg/kg, 총 daidzein은 433 mg/kg으로 aglycones 비율이 61.96%이었고 유리 genistein이 137 mg/kg, 총 genistein은 200 mg/kg으로 aglycones 비율이 68.52%이었으며 daidzein/genistein ratio는 2.16였다. 된장은 유리 daidzein이 578 mg/kg, 총 daidzein은 538 mg/kg으로 aglycones 비율이 107.68%이었고 유리 genistein이 455 mg/kg, 총 genistein은 538 mg/kg으로 aglycones 비율이 85.26%이었으며 daidzein/genistein ratio는 1.00이었다. ${\beta}-glucosidase$의 활성은 콩(5.65 units/mg protein), 메주(2.04 units/mg protein), 된장(0.69 units/mg protein)의 순이었다. pH변화에 따른 ${\beta}-glucosidase$ 활성 변화를 조사한 결과 콩은 pH 6.5, 메주와 된장은 pH 7.0에서 최대 활성을 나타냈다. 온도에 있어서는 $50^{\circ}C$에서 최대 활성을 보였다.