[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1305.05011

High ${\beta}$ -Glucosidase Secretion in Saccharomyces cerevisiae Improves the Efficiency of Cellulase Hydrolysis and Ethanol Production in Simultaneous Saccharification and Fermentation

Tang, Hongting (State Key Laboratory of Microbial Technology, Shandong University)
Hou, Jin (State Key Laboratory of Microbial Technology, Shandong University)
Shen, Yu (State Key Laboratory of Microbial Technology, Shandong University)
Xu, Lili (State Key Laboratory of Microbial Technology, Shandong University)
Yang, Hui (State Key Laboratory of Microbial Technology, Shandong University)
Fang, Xu (State Key Laboratory of Microbial Technology, Shandong University)
Bao, Xiaoming (State Key Laboratory of Microbial Technology, Shandong University)

Publication Information

Journal of Microbiology and Biotechnology / v.23, no.11, 2013 , pp. 1577-1585 More about this Journal

Abstract

Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of ${\beta}$ -glucosidase. In this study, three ${\beta}$ -glucosidases from different organisms were respectively expressed in Saccharomyces cerevisiae and the ${\beta}$ -glucosidase from Saccharomycopsis fibuligera showed the best activity (5.2 U/ml). The recombinant strain with S. fibuligera ${\beta}$ -glucosidase could metabolize cellobiose with a specific growth rate similar to the control strain in glucose. This recombinant strain showed higher hydrolysis efficiency in the cellulose simultaneous saccharification and fermentation, when using the Trichoderma reesei cellulase, which is short of the ${\beta}$ -glucosidase activity. The final ethanol concentration was 110% (using Avicel) and 89% (using acid-pretreated corncob) higher than the control strain. These results demonstrated the effect of ${\beta}$ -glucosidase secretion in the recombinant S. cerevisiae for enhancing cellulosic ethanol conversion.

Keywords

${\beta}$ -Glucosidase; Saccharomyces cerevisiae; cellulase; simultaneous saccharification; fermentation; ethanol;

Citations & Related Records

Reference

1	Stenberg K, Bollok M, Reczey K, Galbe M, Zacchi G. 2000. Effect of substrate and cellulase concentration on simultaneous saccharification and fermentation of steam-pretreated softwood for ethanol production. Biotechnol. Bioeng. 68: 204-210. DOI ScienceOn
2	Sun Y, Cheng J. 2002. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour. Technol. 83: 1-11. DOI ScienceOn
3	van Rooyen R, Hahn-Hagerdal B, La Grange DC, van Zyl WH. 2005. Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains. J. Biotechnol. 120: 284-295. DOI ScienceOn
4	Wilde C, Gold N, Bawa N, Tambor J, Mougharbel L, Storms R, Martin VJ. 2012. Express of a library of fungal $\beta$ -glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain. Appl. Microbiol. Biotechnol. 95: 647-659. DOI ScienceOn
5	Yazdi MT, Woodward JR, Radford A. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136: 1313-1319. DOI ScienceOn
6	Zhang L, Guo Z, Hong J, Ding Z, Gao Z, He Z, et al. 2012. Expressing $\beta$ -glucosidase from Saccharomycopsis fibuligera in industrial ethanol producing yeast and evaluation of the expressing sufficiency. Ann. Microbiol. 62: 539-544. DOI
7	Den Haan R, Rose SH, Lynd LR, van Zyl WH. 2007. Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab. Eng. 9: 87-94. DOI ScienceOn
8	Andric P, Meyer A, Jensen P, Dam-Johansen K. 2010. Effect and modeling of glucose inhibition and in situ g luc ose removal during enzymatic hydrolysis of pretreated wheat straw. Appl. Biochem. Biotechnol. 160: 280-297. DOI ScienceOn
9	Berghem LE, Pettersson LG. 1974. The mechanism of enzymatic cellulose degradation. Isolation and some properties of a beta-glucosidase from Trichoderma viride. Eur. J. Biochem. 46: 295-305. DOI ScienceOn
10	Bezerra RM, Dias AA. 2005. Enzymatic kinetic of cellulose hydrolysis: inhibition by ethanol and cellobiose. Appl. Biochem. Biotechnol. 126: 49-59. DOI ScienceOn
11	Drissen RET, Maas RHW, Tramper J, Beeftink HH. 2009. Modelling ethanol production from cellulose: separate hydrolysis and fermentation versus simultaneous saccharification and fermentation. Biocatal. Biotransform. 27: 27-35. DOI ScienceOn
12	Du F, Wolger E, Wallace L, Liu A, Kaper T, Kelemen B. 2010. Determination of product inhibition of CBH1, CBH2, and EG1 using a novel cellulase activity assay. Appl. Biochem. Biotechnol. 161: 313-317. DOI ScienceOn
13	Entian K-D, Kötter P. 1998. 23 Yeast mutant and plasmid collections, p. 431-449. In Alistair JPB, Mick T (eds.). Methods in Microbiology, Academic Press.
14	Fan Z, McBride JE, van Zyl WH, Lynd LR. 2005. Theoretical analysis of selection-based strain improvement for microorganisms with growth dependent upon extracytoplasmic enzymes. Biotechnol. Bioeng. 92: 35-44. DOI ScienceOn
15	Gibson DG. 2011. Enzymatic assembly of overlapping DNA fragments. Meth. Enzymol. 498: 349-361. DOI
16	Ha S-J, Galazka JM, Kim SR, Choi J-H, Yang X, Seo J-H, et al. 2011. Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation. Proc. Natl. Acad. Sci. USA 108: 504-509. DOI ScienceOn
17	Hahn-Hagerdal B, Wahlbom CF, Gardonyi M, Zyl W, Otero RC, Jonsson L. 2001. Metabolic engineering of Saccharomyces cerevisiae for Xylose Utilization, p. 53-84. In Nielsen J, Eggeling L, Dynesen J, Gardonyi M, Gill RT, Graaf AA, et al. (eds.). Metabolic Engineering, Springer Berlin Heidelberg.
18	Gietz RD, Schiestl RH, Willems AR, Woods RA. 1995. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11: 355-360. DOI ScienceOn
19	Gurgu L, Lafraya A, Polaina J, Marin-Navarro J. 2011. Fermentation of cellobiose to ethanol by industrial Saccharomyces strains carrying the beta-glucosidase gene (BGL1) from Saccharomycopsis fibuligera. Bioresour. Technol. 102: 5229-5236. DOI ScienceOn
20	Gusakov AV, Sinitsyn AP, Manenkova JA, Protas OV. 1992. Enzymatic saccharification of industrial and agricultural lignocellulosic wastes. Appl. Biochem. Biotechnol. 34-35: 625-637. DOI
21	Hari Krishna S, Chowdary GV. 2000. Optimization of simultaneous saccharification and fermentation for the production of ethanol from lignocellulosic biomass. J. Agric. Food Chem. 48: 1971-1976. DOI ScienceOn
22	Hou J, Tyo K, Liu Z, Petranovic D, Nielsen J. 2012. Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae. Metab. Eng. 14: 120-127. DOI ScienceOn
23	Ilmen M, den Haan R, Brevnova E, McBride J, Wiswall E, Froehlich A, et al. 2011. High level secretion of cellobiohydrolases by Saccharomyces cerevisiae. Biotechnol. Biofuels. 4: 30. DOI ScienceOn
24	Nagar S, Gupta VK, Kumar D, Kumar L, Kuhad RC. 2010. Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation. J. Ind. Microbiol. Biotechnol. 37: 71-83. DOI ScienceOn
25	Liu Z, Tyo KE, Martinez JL, Petranovic D, Nielsen J. 2012. Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae. Biotechnol. Bioeng. 109: 1259-1268. DOI ScienceOn
26	Lynd LR, van Zyl WH, McBride JE, Laser M. 2005. Consolidated bioprocessing of cellulosic biomass: an update. Curr. Opin. Biotechnol. 16: 577-583. DOI ScienceOn
27	Philippidis G, Spindler D, Wyman C. 1992. Mathematical modeling of cellulose conversion to ethanol by the simultaneous saccharification and fermentation process. Appl. Biochem. Biotechnol. 34-35: 543-556. DOI
28	Merino S, Cherry J. 2007. Progress and challenges in enzyme development for biomass utilization, pp. 95-120. In Olsson L (ed.). Biofuels. Vol. 108. Springer Berlin-Heidelberg.
29	Nieves RA, Ehrman CI, Adney WS, Elander RT, Himmel ME. 1997. Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol. World J. Microbiol. Biotechnol. 14: 301-304. DOI
30	Peng B, Shen Y, Li X, Chen X, Hou J, Bao X. 2012. Improvement of xylose fermentation in respiratory-deficient xylose-fermenting Saccharomyces cerevisiae. Metab. Eng. 14: 9-18. DOI ScienceOn
31	Philippidis GP, Smith TK, Wyman CE. 1993. Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process. Biotechnol. Bioeng. 41: 846-853. DOI ScienceOn
32	Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA, et al. 2006. The path forward for biofuels and biomaterials. Science 311: 484-489. DOI ScienceOn
33	Spindler D, Wyman C, Grohmann K, Mohagheghi A. 1989. Simultaneous saccharification and fermentation of pretreated wheat straw to ethanol with selected yeast strains and β- glucosidase supplementation. Appl. Biochem. Biotechnol. 20-21: 529-540. DOI
34	Sadie C, Rose S, Haan R, Zyl W. 2011. Co-expression of a cellobiose phosphorylase and lactose permease enables intracellular cellobiose utilisation by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 90: 1373-1380. DOI ScienceOn
35	Shen Y, Zhang Y, Ma T, Bao X, Du F, Zhuang G, et al. 2008. Simultaneous saccharification and fermentation of acid-pretreated corncobs with a recombinant Saccharomyces cerevisiae expressing beta-glucosidase. Bioresour. Technol. 99: 5099-5103. DOI ScienceOn
36	Shi SL, FW He. 2008. Analysis and Measurement of Pulp and Paper (in Chinese). Chinese Light Industry Press, Beijing.

Reference

None	(2013) Biotechnology for biofuels Development of cellobiose-degrading ability in <I>Yarrowia lipolytica</I> strain by overexpression of endogenous genes / 8 (None) , 109
None	(2016) Scientific reports Oligomerization as a strategy for cold adaptation: Structure and dynamics of the GH1 β-glucosidase from <I>Exiguobacterium antarcticum</I> B7 / 6 (None) , 23776
1	(2016) 3 Biotech Catalytic properties, functional attributes and industrial applications of β-glucosidases / 6 (1) , 3
7	(2013) World journal of microbiology & biotechnology Synergies in coupled hydrolysis and fermentation of cellulose using a Trichoderma reesei enzyme preparation and a recombinant Saccharomyces cerevisiae strain / 33 (7) , 140
46	(2013) Genome announcements Draft Genome Sequence of <I>Saccharomycopsis fodiens</I> CBS 8332, a Necrotrophic Mycoparasite with Biocontrol Potential / 5 (46) , e01278-17
None	(2013) Scientific reports Co-fermentation using Recombinant <I>Saccharomyces cerevisiae</I> Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol / 7 (None) , 4428
None	(2013) Microbial cell factories Profiling of <I>Saccharomyces cerevisiae</I> transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion / 16 (None) , 199
None	(2013) Biotechnology for biofuels Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases in <I>Saccharomyces cerevisiae</I> / 10 (None) , 53
None	(2018) Frontiers in microbiology CRISPR-Cas9 Approach Constructing Cellulase <I>sestc-</I> Engineered <I>Saccharomyces cerevisiae</I> for the Production of Orange Peel Ethanol / 9 (None) , 2436
2	(2013) 微生物前沿 Progress of β-Glucosidase from Microorganisms / 7 (2) , 79
None	(2013) Microbial cell factories Efficient yeast surface-display of novel complex synthetic cellulosomes / 17 (None) , 122
5	(2019) Applied microbiology and biotechnology Development of conditional cell lysis mutants of Saccharomyces cerevisiae as production hosts by modulating OCH1 and CHS3 expression / 103 (5) , 2277
2	(2013) Biotechnologia acta FERMENTATION OF SUGARCANE BAGASSE HYDROLYSATES BY Mucor indicus / 12 (2) , 71
14	(2013) Applied microbiology and biotechnology Identification of a thermostable fungal lytic polysaccharide monooxygenase and evaluation of its effect on lignocellulosic degradation / 103 (14) , 5739
13	(2013) Cellulose Coexpression of β-xylosidase and xylose isomerase in Saccharomyces cerevisiae improves the efficiency of saccharification and fermentation from xylo-oligosaccharides / 26 (13) , 7923
None	(2019) Biotechnology for biofuels A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol / 12 (None) , 240
1	(2013) Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] Heterologous secretory expression of β-glucosidase from Thermoascus aurantiacus in industrial Saccharomyces cerevisiae strains / 51 (1) , 107
10	(2020) Sn applied sciences Lignocellulosic biomass and industrial bioprocesses for the production of second generation bio-ethanol, does it have a future in Algeria? / 2 (10) , 1680
10	(2013) Journal of microbiology and biotechnology Transcription Analysis of Recombinant Trichoderma reesei HJ-48 to Compare the Molecular Basis for Fermentation of Glucose and Xylose / 30 (10) , 1467

KSCI

High -Glucosidase Secretion in Saccharomyces cerevisiae Improves the Efficiency of Cellulase Hydrolysis and Ethanol Production in Simultaneous Saccharification and Fermentation

High ${\beta}$ -Glucosidase Secretion in Saccharomyces cerevisiae Improves the Efficiency of Cellulase Hydrolysis and Ethanol Production in Simultaneous Saccharification and Fermentation