• 제목/요약/키워드: $[U-^{14}C]\

Search Result 550, Processing Time 0.033 seconds

Effect of the changes in the Ratio of Dietary Fat to carbohydrate and Energy Restriction on Insulin Sensitivity in Rats (식이중 지방 및 탄수화물의 비율과 열량제한이 흰쥐의 인슐린 민감도에 미치는 영향)

  • 선영실
    • Journal of Nutrition and Health
    • /
    • v.22 no.4
    • /
    • pp.266-274
    • /
    • 1989
  • The current study was undertaken to determine the effects of the ratios of dietary fat to carbohydrate and energy restriction on insulin sensitivity in the growing rats. Male rats weighting 80-90g were fed experimental diets for two weeks. Rats were killed and epiddymal adipose tissue were removed and sliced. Explants of adipose tissues were incubated for 2 hours in KRB(Krebs-Ringer bicarbonate) buffer containing various concentrations of human insulin and [U-14C]glucose. Insulin sensitivity was determined as glucose conversion to total lipids (lipogenesis) during 2 hr incubation. Exp't I : Effects of Ratios of Fat to Carbohydrate on Insulin Sensitivity. Eighteen male rats were fed 3 diets for 2 weeks. Diet 1 was low fat-high carbohydrate (4% soybean oil and 66.5% cornstarch) ; diet 2, medium fat-medium sarbohydrate(12% soybean oil and 58.5% cornstarch) ; diet 3, high fat-low carbohydrate (20% soybean oil and 50.5% cornstarch). Insulin sensitivity was higher in the order of LF-HC, MF-HC and HF-LC diet groups (p<0.05), i.e, lipogenesis was higher at all insuline concentration in the explants from rats fed LF-HC diet. However, thers was no significant difference in body weight gain and epididymal adipose tissue weight among treatments. Exp't II ; Effects of Energy Restriction on Insulin Sensitivity. Twelve rats were grouped into ad libitum feeding and restricted feeding(70% of ad libitum). The experimental diet was medium fat-medium carbohydrat diet as used in the Exp't I. Restricted feeding group tended to show higher insulin sensitivity compared to ad libitum group. However, there was no statistical difference between two groups. As expected, body weight gain and epididymal adipose tissue were higher in the ad libitum group. In summary, the resutls of the current study showed that the epididymal adipose tissue taken from the rats fed low fat-high carbohydrate diet showed higher insulin sensitivity compared to those fed high fat-low carbohydrate, and that resticted feeding tended to elevate insulin sensitivity in these tissues.

  • PDF

A Comparative Study on Internal Fixation Using Long Proximal Intramedullary Nail for the Treatment of Humeral Shaft Fracture according to Fracture Types

  • Choi, Chang-Hyuk;Jun, Chung-Mu;Kim, Jun-Young
    • Clinics in Shoulder and Elbow
    • /
    • v.22 no.2
    • /
    • pp.87-92
    • /
    • 2019
  • Background: This study was conducted to compare the radiological and clinical outcomes of internal fixation using a Polarus humeral nail for treatment of a humeral shaft fracture according to fracture types. Methods: From 43 patients, 13 were excluded and 30 patients were included. The 30 patients were divided into 2 groups: 15 in group I (Orthopaedic Trauma Association/Arbeitsgemeinschaft $f{\ddot{u}}r$ Osteosynthesefragen classification type A and B) and 15 in group II (type C). The mean age was 63.1 years (range, 20-87 years), and mean follow-up period was 2.3 years (range, 1.0-6.1 years). The causes of injuries were as follows: 12, traffic accidents; 14, simple slips; 2, simple falls; 2, contusions after lower energy trauma. Radiological and clinical evaluations were performed. Results: Radiological union was confirmed by plain anteroposterior and lateral radiographs on average of 5.0 months in group I, and 8.4 months in group II, respectively. Differences between the two groups were statistically significant (p<0.01). The clinical union value was 1.6 in group I, and 2.0 months in group II, but these values did not differ significantly (p=0.441). The mean Korean shoulder scoring system scores were 89.7 and 90.6, which did not differ significantly (p=0.352). Conclusions: Intramedullary nailing using the Polarus humeral nail is considered to be a good treatment modality for all types of humeral shaft fractures. Additionally, the Polarus humeral nail can be an optimal choice for the treatment of complex type fractures such as segmental or comminuted humeral shaft fractures.

Compare of Characteristics of Efficient and Inefficient Problem-Solving Teams Using SYMLOG Diagram Analysis (SYMLOG 다이어그램 분석을 통한 효율적인 문제 해결 팀과 비효율적인 문제 해결 팀의 특징 비교 연구)

  • Jo, Han-jin;Lee, Hakseok;Shin, Gyeong-Seop;Kim, Taehoon
    • Journal of Engineering Education Research
    • /
    • v.24 no.1
    • /
    • pp.3-14
    • /
    • 2021
  • The purpose of this study is to analyze the characteristics of efficient problem-solving teams and inefficient problem-solving teams using SYMLOG. In this study, 35 college students majoring in engineering education at C university were organized into 7 teams and carried out technological problem solving projects over one semester. Based on the results of the team project, the top 2 teams were defined as efficient problem solving teams and the bottom 2 teams were defined as inefficient problem solving team, and analyzed the characteristics of the team using SYMLOG. The main results are as follows: First, an analysis of SYMLOG from efficient problem solving teams and inefficient problem solving teams showed that there was a difference between self-awareness and others' perception in terms of U(Upward)-D(Downward) dimension. Second, in the inefficient problem solving teams, there was a significant difference between self-awareness and others' in the F(Forward)-B(Backward) dimension. Third, there was no difference between self-awareness and others' in both efficient and inefficient teams at the P (Positive)-N(Negative) dimension. Fourth, an efficient problem-solving team had a clear leader, and there was a team member who supported the leader. On the other hand, the inefficient problem-solving team did not have a clear leader, or one person played the role of leader and there were no team members supporting the leader.

On the Analysis of Physical Distribution System in Mokpo Port (목포항 물류시스템의 분석에 관한 연구)

  • Lee, C. Y.;Nam, M. U.
    • Journal of Korean Port Research
    • /
    • v.10 no.1
    • /
    • pp.1-14
    • /
    • 1996
  • Rapid change in the technological environment of marine transportation and the development of the ocean shipping industry have fostered a revolution in the port system. This in turn has caused major changes in the function and use of port in Korea. Aside from this, Mokpo Port, however continues to decline, because the existing port facilities and related subsystem are already obsolete with no chance of regaining operational effectiveness and treatment for proper implementation. Although a few studies have been done on the Mokpo Port, has not been found, any reseach for the analytical approach to the transportation system of it. This paper aims to make an extensive analysis of the physical distribution system in Mokpo Port focusing on the coordination of subsystems such as navigational aids system. The base of introduced simulation tool here is the queueing theory. The overall findings are as follows: 1. Among those vessels called at Mokpo Port in 1994, 556 ships(2,736,669 G/T) are oceangoing while 8155 ships(2,587,217 G/T) are domestic. The average size of oceangoing vessels is 4,922,1 G/T, and the domestic is 317,8 G/T. The average arrival interval and service time of the domestic vessels are 6.0 hours and 24.1 hours respectively marking the berth occupation rate over 100%. Those for oceangoing vessels are 34.5 hours, 120.0 hours and 37.2%. In order to maintainin the berth occupation rate to 70% the capacity considering the 1994 of domestic piers must be extended to 145% and oceangoing vessels must be increased to 165%. 2. The capacity of approaching channel is enough to handle the total traffic volume of 3. Tugs are sufficiently being provided to handle all ships requiring their services 4. The capacity of storage and inland transportation systems are sufficient to handle the throughput and the yard stroage utilization rate of No.1 - No.5 is 4.5% and No.6 is 30% of 1993's. 5. The utilization rate of LLC(Level Looping Crane) and PNT(PNeumaTic) are 2.7% and 18.8%, respectively.

  • PDF

Molecular Cloning and Functional Expression of Extracellular Exo-β-(1,3)-Glucanase from Candida fermentati SI (Candida fermentati SI의 exo-β-(1,3)-glucanase유전자의 클로닝 및 그 특성)

  • Lim, Yu-Mi;Kim, Bong-Ki;Kim, Sang-Jun;So, Jai-Hyun;Kim, Won-Chan
    • Microbiology and Biotechnology Letters
    • /
    • v.44 no.3
    • /
    • pp.317-323
    • /
    • 2016
  • An isoflavone glucosidase that catalyzes the hydrolysis of isoflavone glucosides into glucose and corresponding aglycones was purified from Candida fermentati SI. The N-terminal sequence was determined to be GLNCDYCN. We designed degenerate primers on the basis of these amino acid sequences and successfully cloned the full structural gene sequence of the isoflavone glucosidase using inverse PCR. The exo-β-(1,3)-glucanase gene consists of 1227 base-pair nucleotides, encoding a 408-amino-acid sequence that shares 41–96% amino acid homology with other yeast exo-β-(1,3)-glucanases belonging to glycoside hydrolase family 5. The recombinant exo-β-(1,3)-glucanase was expressed in Pichia pastoris X-33, using a pPICZA vector system, and further characterized. The molecular mass of the purified exo-β-(1,3)-glucanase was estimated by SDS-PAGE to be 47 kDa. The optimal pH and temperature were pH 4.5 and 40℃, respectively. The Km values of the purified exo-β-(1,3)-glucanase for daidzin and genistin were 0.12 mM and 0.14 mM, respectively. The Vmax values of the purified isoflavone glucosidase were 945.03 U/mg for daidzin and 835.92 U/mg and for genistin.

Petrochemistry of the Hongcheon Fe-REE ore deposit in the Hongcheon area, Korea (홍천 철-희토류광상 모암의 암석화학)

  • 박중권;이한영
    • The Journal of the Petrological Society of Korea
    • /
    • v.12 no.3
    • /
    • pp.135-153
    • /
    • 2003
  • In order to understand its origin and petrogenesis, petrochemical studies of major, trace elements, REE, and stable isotopes of oxygen and carbon from the Hongcheon Fe-REE deposits have been investigated. The Hongcheon Fe-REE deposit intruding into Precambrian metasedimentary rocks consists of magnetite, various carbonates such as ankerite, siderite, magnesite and strontianite, monazite, aegirine-augite, Na-amphibole, and sulfides. Compared with major elements abundances of typical ferro-carbonatites, the Hongcheon Fe-REE deposit is enriched in FeO and depleted in CaO with increasing of $SiO_2$, where $TiO_2$and $A1_2O_3$increased and CaO, FeO, MgO and $P_2O_5$ are slightly decreased, but those are rather scattered and their trends are somewhat ambiguous. V Ni, U and Rb slightly increasing with of $SiO_2$increase and scattering or no trends of other detected elements. Nb, Zr and Zn are depleted then the abundances of typical ferro-carbonatites (Woolley and Kempe, 1989). In rare earth elements a large enrichment of total REE (maximum 14.8 wt%) and LREE relative to chondrites and HREE depleted more then the values of ferro-carbontites therefore La/Lu ratios shows large abundances (max. 16,197). The results of stable isotopes of O and C from minerals of ankerite and strontianite and whole rocks represent studied rocks are from igneous carbonatitic melts. Although petrochemical characteristics of the Hongcheon Fe-REE deposits are somewhat different from normal ferro-carbonatites from the world, this discrepancy suggests another conclusion that petrochemical characteristic of the studied Fe-REE mineralized rocks are similar to those of phoscorites from Kovdor, Russia and Sokli, Finland showing the same petrochemical compositions described above.

A Study on the Fluctuation and Influential factors of Daily Visitors of Seoul Children′s Grand Park (도시공원 이용자수의 변동특성과 그 영향변인에 관한 연구 -서울 어린이대공원을 대상으로-)

  • 엄붕춘;최준수
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.14 no.2
    • /
    • pp.81-90
    • /
    • 1986
  • The full grasp of recreation demand and factors affecting on recreation demand can be very important information for park planning and management. The object-tives of this study are to investigate factors affecting the fluctuation of urban park visitors and to analyze the relationship between these factors and the daily parti-cipations. The results were as follows; 1) The peak of monthly participations comes on May, April, August and October in order. And these months are specified as school picnic period and vacation of school children. 2) In correlation analysis, the variables such as ‘Day of a week(D)’, ‘Monthly mean temp.(T)’and ‘Monthly character(M)’have high correlations with ‘No. of visitors’in order. And it is better to categorize months by its charater(picnic period in school, vacation etc) than by seasons. 3) Candidate regression model were established, as for 1984 log U= 1.51 + 0.64D1 + 0.02T + 0.36W1 - 0.23M4 + 0.003SS + 0.24Ml($R^2$=0.5326) where, U=no. of daily visitors D1 = sunday.ho1iday(1), weekday(0) T=monthly mean temperature($^{\circ}C$) W1= weather (sunny.cloudy(1) , rainy (>5mm)(0)> M4=non vacations and non school picnic period(1) , if not (0) SS=monthly sunshining hours M1=summer vacation(1), if not(0) 4) The most important variable was ‘Day of a week’(sunday.holiday or not). And temperature, weather and monthly charcter(especially picnic period of school and vacation) were in turn, hence ‘Children's grand park’shows the use pattern of park.

  • PDF

Liver function in Clonorchis sinensis-infected rabbits (간흡충을 감염시킨 토끼의 간기능 검사)

  • 홍성종;우호춘
    • Parasites, Hosts and Diseases
    • /
    • v.32 no.3
    • /
    • pp.177-184
    • /
    • 1994
  • Nine rabbits were fed with Clonorchis sinenis metacercariae (MC) and the blood samples chronologically obtained were analyzed biochemically. Rabbits Infected by less than 100 flukes were grouped into Group I, and by 100-250 flukes into Group II. The serum level of alanine amlnotransferase (ALT) was increased from 3 weeks after the Infection of the metacercariae (AIM) and showed a peak at 8 weeks, and decreased from 12 weeks ASM. The serum level of aspartate aminotransferase (AST) was raised to $92.3{\;}{\pm}{\;}65.4{\;}U/L$ at 3 weeks AIM and stayed high until 8 weeks, then lowered thereafter. The serum level of ${\gamma}-glutamyl$ transpeptidase (${\gamma}-GT$) was increased rapidly to the highest value ($18.9{\;}{\pm}{\;}14.6{\;}U/L$) at 16 weeks ASM, and decreased to the control level after 20 weeks. The serum level of alkaline phosphatase (ALP) was headed down from the early infection to 52 weeks AIM. The serum cholesterol level was increased from 8 weeks and reached at a peak 16 weeks AIM, and decreased thereafter to the control level. It is suggested that serum ALT, AST, ALP and ${\gamma}-GT$ tests be useful to diagnose the early infEction of C. sinensis.

  • PDF

Stress Reduction Effect of Buddhism and Mind Healing Lectures Measured by QEEG (정량뇌파(QEEG)로 측정한 불교와 마음치유 강의의 스트레스 저감 효과)

  • Kim, Jun-Beom;Hwang, Joon-Sung;Weon, Hee-Wook
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.22 no.1
    • /
    • pp.585-594
    • /
    • 2021
  • This Quasi-experimental study was started under the assumption that the stress of students who participated in Buddhism and Mind Healing Lectures based on an understanding of the scriptures will be relieved through the lectures, thereby enhancing their psychological stability, thinking ability, and enhancing understanding. Stress can be confirmed through a self-report test, but in this study, quantitative EEG was measured to evaluate the stress level and secure objectivity. To this end, the difference between the 1st week as pre and 15th week as post quantitative EEG was verified for the experimental group taking the Buddhism and Mind Healing Lecture held from March to June 2019 at S University in G-gu, Seoul, and the control group who did not. The Mann Whitney U test and Wilcoxon code ranking test were used as analysis methods because the number of subjects was 14. As a result, there was a significant difference in the beta wave (F7, T3, 4, T5) and the high beta wave (F7, F8, T3, T4) in the experimental group. The coherence was also improved, while there was no significant difference in the control group. Buddhism and Mind Healing Lectures improved stress.

HYDROLYTIC DEGRADATION OF POSTERIOR RESIN RESTORATIVE MATERIALS (구치부 레진 수복 재료의 가수분해)

  • Yang, Kuy-Ho;Park, Mi-Ran;Choi, Nam-Ki;Park, Eun-Hae
    • Journal of the korean academy of Pediatric Dentistry
    • /
    • v.28 no.4
    • /
    • pp.673-682
    • /
    • 2001
  • The use of resin composites has continued to increase over the last several years. In spite of their growing popularity, composites continue to exhibit a number of undesirable characteristics. One of the major deficiencies of composite restorative resins is their inadequate resistance to wear. Of the multitude of factors that have been associated with wear, subsurface degradation within the restoration is considered to be one. The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. This solution with a high concentration of hydroxyl ions is a convenient medium for accelerated degradation of silane coupling and filler particles. The brands studies were Definite($Degussa-H\ddot{u}ls$ AG, Germany), Prodigy(Kerr, USA), Pyramid(Bisco, USA) and Synergy(Coltene, Swiss). Preweighed discs of each brand were exposed to 0.1N NaOH solution at $60^{\circ}C$. After 14 days they were removed, neutralized with HCl, washed with water and dried. Resistance to degradation was evaluated on the basis of following parameters : (a) mass loss(%)-determined from pre-and post-exposed specimen weights : (b) Si loss(ppm)-obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation $depth({\mu}m)$-measured microscopically (SEM) from polished circular sections of exposed specimens. The results were follows: 1. Mass loss of Synergy was $1.24{\pm}0.002%$, it was the highest, there was no significant difference among the materials. 2. The degree of degradation layer depth of Synergy was $107.83{\pm}2.52{\mu}m$, it was the highest, there was no significant difference among any other materials than Synergy. 3. There was no difference among the four materials in Si loss. 4. The correlation coefficient between mass loss and degradation depth was relatively high(r=0.06, p<0.05). 5. There was no coefficient correlation between Si loss and mass loss, the degree of degradation layer depth and Si loss. 6. When observed with SEM, destruction of bonding is observed between resin matrix and filler.

  • PDF