• Title/Summary/Keyword: $[^{13}N]NH_3$

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Effect of Drought Stress on the Concentration of Nitrogen Metabolites in White Clover

  • Kim, Tae-Hwan;Lee, Bok-Rye;Jung, Woo-Jin;Kim, Dae-Hyun;Kim, Kil-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.2
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    • pp.95-101
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    • 2002
  • To investigate the changes in the composition and pool size of nitrogen metabolites under drought stress, white clover (Trifolium repens L.) were exposed to -0.04MPa (well-watered, control) or to -0.12MPa (drought-stressed) of soil water potential during 28 days. Dry weight of leaves in drought-stressed plants was remarkably decreased by 45% and 74% within 14 days and 28 days, respectively, compared with control. For nitrate concentration after 28 days of treatment, a significant difference (1.6 times higher in drought-stressed plants) was observed only in stolon. NH$_3$-NH$_4$$^{+}$ concentration in all three organs of drought-stressed plants linearly increased to more than 1.6 times higher level at 28 day when compared to the initial level (day 0), while the increasing rate in control was much less than that of drought-stressed plants. Proline concentrations in drought-stressed plants remarkably increased and reached to 7, 13 and 17 times higher level at 28 day compared to control. Protein concentration in leaves of drought-stressed plants tended to decrease, while it slightly increased during the first 14 days and reached a plateau afterward in control. There was not significant difference in the proteins concentration of stolon and roots throughout experimental period. On SDS-PAGE analysis, two major proteins specifically induced by drought stress (16-kD and 18-kD) were detected in stolon.n.

Study on the Discharge Characteristics of Non-point Pollutant Source in the Farming Area (농촌지역의 비점오염원 유출 특성에 관한 연구)

  • Gil, Kyung-Ik;Lee, Byung-Soo;Lee, Sang-Soo;Park, Moo-Jong
    • 한국방재학회:학술대회논문집
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    • 2008.02a
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    • pp.783-786
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    • 2008
  • The main goal of this study is to understand the effects of direct-runoff of chemistry and organic fertilizers which are adsorbing to sediment from farmland and non-point source discharge characteristics which are discharged to stream with soil erosion when rainfalls. pollutographs of TSS, BOD, COD, TN, and TP were measured for 10 rainfall events at watershed. EMC (Event Mean Concentration) were calculated for each rainfall event using quality and quantity measured. The result shows that the EMC ranges of 95% confidence intervals are 50.5-203 mg/L for TSS, 0.8-14.2 mg/L for $BOD_5$, 4.2-20.7 mg/L for $COD_{Mn}$, 0.2-0.5 mg/L for TP, 2.4-4.5 mg/L for TN, 1.36-3.04 mg/L for NO3--N, 0.13-0.42 mg/L for NH4+-N and 0.82-1.77 mg/L for TKN.

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Analysis of Contributing Factor for Cation Ratio to Calcium in Nutrient Solution on the Incidence of Blossom-end Rot in Sweet Pepper 'RZ208' Grown in Hydroponics (파프리카 배꼽썩음과 발생에 미치는 배양액 내 칼슘에 대한 양이온 기여인자 분석)

  • Lee, Hye-Jin;Oh, Jeong-Sim;Choi, Ki-Young;Lee, Yong-Beom;Bae, Jong-Hyang;Rhee, Han-Cheol;Kim, Dong-Eok
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.27-33
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    • 2012
  • This study aimed to investigate the responses of plant growth and blossom-end rot (BER) incidence to calcium (Ca) and its three kinds of antagonistic cations (K, Mg, and $NH_4$-N) with various ratios in nutrient solution for sweet paper (Capsicum annuum L. 'RZ208'). Both Ca to each cation and Ca to a series of cation combinations, such as potassium (K), ammonium nitrate ($NH_4$), or magnesium (Mg) were more influential to the fruit growth and quality than plant growth. Especially, the BER incidence was significantly influenced by the ratio treatments. For examples, when Ca:(K + Mg) or Ca:(K + Mg + $NH_4$) ratio was 1:2 the highest incident rate of BER about 70.3 or 86.3% was observed, lowering the marketable yield to 19 or 13.7% of the total yield, respectively. The correlation coefficiencies (= r) to relationships between the BER and K as well as BER and $NH_4$ were 0.82 (P < 0.05) and 0.65 (P < 0.05), respectively. Combination only with the Mg element was not correlated with the BER incidence. However, when both of the K and Mg concentrations were 0.65 (P < 0.05). The highest correlation coefficiency, 0.92 (P < 0.05), was found to a relationship between the BER and the tree elemental combination.

A Study on Shelf-life of Seasoned and Fermented Squid (오징어 조미젓갈의 품질유지기한에 관한 연구)

  • KIM Dong-Soo;KIM Young-Myoung;KOO Jae-Guen;LEE Young-Chul;DO Jeong-Ryong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.1
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    • pp.13-20
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    • 1993
  • Traditional fermented fish products 'Jeodtgal' have been prepared with addition of about $25\%$ salt to prevent spoilage, but domestic seasoned and fermented products have only about $8\%$ salt, so they have been required careful treatment during distribution and could have a different fermentation pattern from traditional 'Jeodtgal' This study was conducted to establish shelf-life of seasoned and fermented squid at different distribution conditions. To establish shelf-life of seasoned and fermented squid stored at $10^{\circ}C,\;20^{\circ}C$ and $30^{\circ}C$, chemical components and sensory evaluation were investigated. Chemical components such as volatile basic nitrogen(VBN), ammonia nitrogen ($NH_3-N$) and total nitrogen(T. N) rapidly increased during storage at $20^{\circ}C$ and $30^{\circ}C$, but pH value and Hunter color value(${\Delta}E$) slowly decreased during storage without respect of storage temperature. Judging from chemical components analysis and senseory evaluation, seasoned and fermented squid could be storage for 3days at $30^{\circ}C$, 7days at $20^{\circ}C$ and 25days at $10^{\circ}C$, respectively. The ratio of quality deterioration per hour were 0.00166 at $10^{\circ}C$, 0.00595 at $20^{\circ}C$ and 0.01385 at $30^{\circ}C$.

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Effect on Nitrous Oxide Emission in Applying Livestock Manure Compost for Strawberry (Fragaria × ananassa Duch.) Cultivation in Plastic Film House (딸기(Fragaria × ananassa Duch.) 시설재배에서 가축분 퇴비 시용이 아산화질소 배출에 미치는 영향)

  • Lee, Chang-Kyu;Moon, Hyung-Cheol;Song, Eun-Ju;Choi, Seon-U;Ko, Do-Young;Chon, Hyong-Gwon;Yun, Seok-In
    • Korean Journal of Organic Agriculture
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    • v.29 no.1
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    • pp.111-123
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    • 2021
  • This study was conducted to investigate the growth characteristics of strawberries and N2O emission by treating the compost for each type of livestock manure, which was an organic farming material, as a basal fertilization in plastic film house. Livestock manure compost, which made from cattle manure, swine manure, and poultry manure as raw materials, were applied to this experiment, treated by mixing or single on the basis of nitrogen content with the standard amount of fertilizer for strawberries. Total emission of N2O were 10.7% higher than those in poultry manure compost treatment compared to the inorganic fertilizer treatment, but 16.5~41.9% lower than those in other livestock manure compost treatment. The period of N2O emission mainly was up to the 17th day after fertilizer application, accounting for 70~87% of the total amount of discharge, and 13~30% of the total amount was emitted for 158 days later. N2O emission was decreased significantly NH4+-N content in the soil, and increased NO3--N. As compared with control, the number of leaves, leaf width and crown diameter of livestock manure compost treatments were not significantly different, leaf length of cattle+poultry, cattle+ swine, swine+poultry treatment higher, and SPAD (soil plant analysis development) values of cattle+poultry treatment highest. There was no significant difference in weight and sugar content of strawberry fruits among treatments.

The Effect of Yeast(Saccharomyces exiguus SJPAF1) on Odor Emission and Contaminants Reduction in Piggery Slurry (효모(Saccharomyces exiguus SJPAF1) 첨가에 따른 돈분뇨의 악취제거 및 오염물질 감소 효과)

  • Yoon, Deok-Hoon;Kang, Dong-Woo;Nam, Ki-Woong
    • Korean Journal of Environmental Agriculture
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    • v.28 no.1
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    • pp.47-52
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    • 2009
  • The aim of this study was to evaluate the effect of yeast(Saccharomyces exiguus SJPAF1, referred to as SA) addition on odor emission and contaminants reduction in piggery sluny. Four different rates of yeast addition were compared: no addition(SA0), 0.7L(SA0.7), 1.0L(SA1.0), and 1.5L(SA1.5) to one tone of piggery slurry. Odor emission tended to decrease with increasing the yeast application with concurrent effects of changes in temperature on outside of reactors. Particularly, reduction in ammonia emission was proportional to the yeast application rate; it reduced from 161.1 ppm in SA0 to 47.1 ppm in SA1.5 after 6 days of treatment Decomposition of piggery shiny by yeast increased to 13.8% more in SA1.5, and total amounts of piggery slurry decreased to 12.5% in SA1.5. Total coliforms were detected below 30MPN $ml^{-1}$ in SA1.5, while $8.3{\times}10^3$ MPN $ml^{-1}$ of Total coliforms were found in SA0. However, the effect of yeast addition in piggery slurry seemed to have no influence on the removal efficiency of contaminants such as BOD, COD, $NO_3^{-}-N$, $NH_4^{+}-N$, $PO_4^{-}P$. Consequently, the yeast(Saccharomyces exiguus SJPAF1) addition of 1.5% in the piggery sluny seems to have potential applicability for improving agent of pig-farm environment.

Spatial assessment of heat wave and river water quality (폭염과 하천 수질의 공간적 평가)

  • Lee, Jiwan;Kim, Sehoon;Han, Daeyoung;Shin, Hyungjin;Lim, Hyeokjin;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.54 no.9
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    • pp.693-704
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    • 2021
  • The purpose of this study is to evaluate the relationship between heat wave and river water quality. The daily maximum air temperature (Tmax) of 91 meteorological stations of the Korea Meteorological Administration and 13 river water quality factors (DO, BOD, COD, TOC, TN, DTN, NH4-N, NO2-N, NO3-N, TP, DTP, PO4-P, Chl-a) of Ministry of Environment were analyzed. The correlation analysis was performed on Tmax and water quality factors, and the determination coefficients (R2) of DO, Chl-a, and TN with Tmax showed high values of 0.782, 0.609, and 0.691 respectively. To analyze the spatial impact between heat waves and water quality factors, the heat wave intensity (HWI) and heat wave duration (HWD) were calculated using the Tmax. The hotspot and spatial statistical analyses were applied for spatial impact evaluation. As a result of hotspot analysis, the heat wave index (HWD, HWI) showed high spatial pattern in the downstream of Nakdong River basin, and Chl-a and TN showed the same pattern. In case of spatial statistical analysis for water quality due to heat wave, the most obvious spatial variability was DO.

The Structure Analysis and Biosynthesis of $\beta$-glucan by Alcaligenes faecalis (Alcaligenes faecalis에 의한 $\beta$-glucan의 생합성과 구조 분석)

  • Ryu, Kang;Lee, Ki-Young;Lee, Eun-Kyu
    • KSBB Journal
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    • v.16 no.4
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    • pp.409-414
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    • 2001
  • Biosynthesis of curdlan( ${\beta}$-1,3 glucan) was shown by fluorscence on cellufluor medium. The highest production of curdlan was produced when glucose was used as a carbon source and ($NH_4$)$_2$$SO_4$ was used as a nitrogen source. ${\beta}$ -form of curdlan was detected in the fingerprint region (890 $cm^{-1}$) by FT-IR spectrum and shown homogeneous ${\beta}$ -1,3 glucan by $^{13}C$ NMR spectrum ($C_1$-103 ppm, $C_2$-73.2 ppm, $C_3$-86.4 ppm, $C_4$-68.7 ppm, $C^{5}$-76.63 ppm, $C_{6}$-61.2 ppm). Transition of structure from triple helix coil form to random coil form was appeared at 0.1 ∼0.25 M NaOH concentration. It was shown that natural curdlan is a triple helix form in neutral but becomes weak in alkaline condition.

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Optimization of gibberellin production by Fusarium prolifertum KGL0401 and its involvement in waito-c rice growth (Fusarium prolifertum KGL0401의 지베렐린 생산 최적조건과 waito-c 생장에 미치는 영향)

  • Rim, Soon-Ok;Lee, Jin-Hyung;Lee, In-Jung;Rhee, In-Koo;Kim, Jong-Guk
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.120-124
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    • 2007
  • Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited higher GA productivity than wild type Gibberella fujikuroi. The :tim of this work was to find out an optimal culture condition for GA production. Various carbon(fructose, glucose, lactose, maltose, sucrose) and nitrogen($KNO_3$, urea, glycine, $NaNO_3,\;NH_4Cl$) sources were used for this study. GAs activities were analysed by gas chromatography and mass spectrometry(GC-MS). The highest yield of $GA_3$ was found in the growth medium supplemented with sucrose as carbon source and $NH_4Cl$ as nitrogen source. The optimum carbon-nitrogen concentration for $GA_3$ production was found to be 0.5 M:0.17 M. Supernatant was prepared from the culture fluid of F. proliferatum KGL0401 cultured for 7 days at 3 0'E and the 10 ul supernatant was treated with 2 leaf-rice seedling.

Solution Nitriding and Its Effect on the Austenitic Stainless Steels (오스테나이트계 스테인리스강에 대한 질소 고용화 처리 및 그 효과)

  • Huh, J.;Nam, T.W.
    • Journal of the Korean Society for Heat Treatment
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    • v.13 no.5
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    • pp.337-345
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    • 2000
  • As a case hardening process for stainless steels, nitriding is more preferred and widely used than carburizing which deterioates corrosion resistance severely. In order to add the nitrogen into the stainless steels, passive film on the surface must be removed effectively before nitriding. Conventional gas nitriding process is performed in the temperature range of 500 to $600^{\circ}C$ with $NH_3$ gas, which often leads to sensitization of stainless steels. In this study, we tried to activate passive film of austenitic stainless steels by heating at low pressure. ($900^{\circ}C$, $5{\times}10^{-2}$ Torr.) Nitriding was performed at the solution treatment temperature of $1100^{\circ}C$ with nitrogen molecules instead of $NH_3$ gas. An attainable nitrogen content in a case depends on the nitrogen gas pressure at constant nitriding temperature. A case depth is proportional to the square root of solution time, which suggests that inward diffusion of nitrogen follows the Fick's 2nd law. Surface nitrogen atoms are dissolved as interstitial solutes, or precipitated in the form of MN, $M_2N$ nitrides, which increase the case hardeness. Dissolved nitrogen in the case enhances the cavitation resistance of austenitic stainless steels dramatically.

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